Restriction map for
pF1KE0239
(Circular) (Six-base) MAPSORT of: pF1KE0239.seq Check: 2903 from: 1 to: 3938
pF1KE0239 3938 bp
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AccI GT'mk_AC
Cuts at: 976 976
Size: 3938
Acc65I G'GTAC_C
Cuts at: 954 954
Size: 3938
AclI AA'CG_TT
Cuts at: 3778 3778
Size: 3938
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 457 704 1763 2195 2942 3210 457
Size: 247 1059 432 747 268 1185
Fragments arranged by size:
1185 1059 747 432 268 247
AflIII A'CryG_T
Cuts at: 1290 2394 3371 1290
Size: 1104 977 1857
Fragments arranged by size:
1857 1104 977
AgeI A'CCGG_T
Cuts at: 2326 2326
Size: 3938
AleI CACnn'nnGTG
Cuts at: 570 570
Size: 3938
AlwNI CAG_nnn'CTG
Cuts at: 1243 2810 1243
Size: 1567 2371
ApaLI G'TGCA_C
Cuts at: 229 439 2708 229
Size: 210 2269 1459
Fragments arranged by size:
2269 1459 210
ApoI r'AATT_y
Cuts at: 622 942 3291 3547 622
Size: 320 2349 256 1013
Fragments arranged by size:
2349 1013 320 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3938
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3938
AvaI C'yCGr_G
Cuts at: 958 958
Size: 3938
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 122 155 122
Size: 33 3905
BamHI G'GATC_C
Cuts at: 963 963
Size: 3938
BanI G'GyrC_C
Cuts at: 766 954 1368 1616 1651 766
Size: 188 414 248 35 3053
Fragments arranged by size:
3053 414 248 188 35
BanII G_rGCy'C
Cuts at: 131 952 1982 131
Size: 821 1030 2087
Fragments arranged by size:
2087 1030 821
BbeI G_GCGC'C
Cuts at: 1372 1620 1372
Size: 248 3690
BbsI GAAGACnn'nnnn_
Cuts at: 463 463
Size: 3938
BbvCI CC'TCA_GC
Cuts at: 490 490
Size: 3938
BciVI GTATCCnnnnn_n'
Cuts at: 1836 2597 3938 1836
Size: 761 1341 1836
Fragments arranged by size:
1836 1341 761
BclI T'GATC_A
Cuts at: 1458 1458
Size: 3938
BglII A'GATC_T
Cuts at: 1453 3490 1453
Size: 2037 1901
BlpI GC'TnA_GC
Cuts at: 352 1050 352
Size: 698 3240
Bme1580I G_kGCm'C
Cuts at: 233 443 771 1563 1656 2712 233
Size: 210 328 792 93 1056 1459
Fragments arranged by size:
1459 1056 792 328 210 93
BmrI ACTGGGnnnn_n'
Cuts at: 1150 1231 1566 3710 1150
Size: 81 335 2144 1378
Fragments arranged by size:
2144 1378 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2305 2305
Size: 3938
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 275 275
Size: 3938
Bpu10I CC'TnA_GC
Cuts at: 490 490
Size: 3938
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1114 1993 2485 2783 1114
Size: 879 492 298 2269
Fragments arranged by size:
2269 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 209 3642 209
Size: 3433 505
BsaAI yAC'GTr
Cuts at: 1291 1291
Size: 3938
BsaBI GATnn'nnATC
Cuts at: 6 1452 3489 6
Size: 1446 2037 455
Fragments arranged by size:
2037 1446 455
BsaHI Gr'CG_yC
Cuts at: 1369 1617 2319 3815 1369
Size: 248 702 1496 1492
Fragments arranged by size:
1496 1492 702 248
BsaWI w'CCGG_w
Cuts at: 1127 1648 2326 2600 2747 3158 1127
Size: 521 678 274 147 411 1907
Fragments arranged by size:
1907 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 918 921 918
Size: 3 3935
BseYI C'CCAG_C
Cuts at: 276 1363 2121 2698 3194 276
Size: 1087 758 577 496 1020
Fragments arranged by size:
1087 1020 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 363 363
Size: 3938
BsiEI CG_ry'CG
Cuts at: 104 1480 1526 2734 104
Size: 1376 46 1208 1308
Fragments arranged by size:
1376 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 131 233 443 609 952 1730 1920 2712 131
Size: 102 210 166 343 778 190 792 1357
Fragments arranged by size:
1357 792 778 343 210 190 166 102
BsmI GAATG_Cn'
Cuts at: 356 356
Size: 3938
Bsp1286I G_dGCh'C
Cuts at: 131 233 443 609 771 952 1563 1656 1730
Size: 102 210 166 162 181 611 93 74
Cuts at: 1730 1920 1982 2712 131
Size: 190 62 730 1357
Fragments arranged by size:
1357 730 611 210 190 181 166 162
102 93 74 62
BspEI T'CCGG_A
Cuts at: 3158 3158
Size: 3938
BspHI T'CATG_A
Cuts at: 3114 3114
Size: 3938
BspMI ACCTGCnnnn'nnnn_
Cuts at: 385 988 1202 1504 1885 385
Size: 603 214 302 381 2438
Fragments arranged by size:
2438 603 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2230 2284 3768 3933 50
Size: 2180 54 1484 165 55
Fragments arranged by size:
2180 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1850 1850
Size: 3938
BsrFI r'CCGG_y
Cuts at: 1936 2117 2326 1936
Size: 181 209 3548
Fragments arranged by size:
3548 209 181
BssSI C'ACGA_G
Cuts at: 2209 2567 2209
Size: 358 3580
BstAPI GCAn_nnn'nTGC
Cuts at: 1315 1315
Size: 3938
BstBI TT'CG_AA
Cuts at: 2299 2299
Size: 3938
BstYI r'GATC_y
Cuts at: 1 963 1430 1453 1788 2034 3046 3132 3144
Size: 962 467 23 335 246 1012 86 12
Cuts at: 3144 3490 1
Size: 346 449
Fragments arranged by size:
1012 962 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 180 180
Size: 3938
BtgI C'CryG_G
Cuts at: 447 447
Size: 3938
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1888 2069 2232 1888
Size: 181 163 3594
Fragments arranged by size:
3594 181 163
BtsI GCAGTG_nn'
Cuts at: 131 380 441 1181 131
Size: 249 61 740 2888
Fragments arranged by size:
2888 740 249 61
DraI TTT'AAA
Cuts at: 937 937
Size: 3938
DrdI GACnn_nn'nnGTC
Cuts at: 1644 2502 1644
Size: 858 3080
EaeI y'GGCC_r
Cuts at: 1523 1697 2088 2115 3460 1523
Size: 174 391 27 1345 2001
Fragments arranged by size:
2001 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1523 1523
Size: 3938
EarI CTCTTCn'nnn_
Cuts at: 184 524 912 1961 2171 184
Size: 340 388 1049 210 1951
Fragments arranged by size:
1951 1049 388 340 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2146 2456 2602 3748 2146
Size: 310 146 1146 2336
Fragments arranged by size:
2336 1146 310 146
EcoICRI GAG'CTC
Cuts at: 129 950 129
Size: 821 3117
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 275 457 704 1763 2195 2942 3210 275
Size: 182 247 1059 432 747 268 1003
Fragments arranged by size:
1059 1003 747 432 268 247 182
EcoNI CCTnn'n_nnAGG
Cuts at: 179 179
Size: 3938
EcoO109I rG'GnC_Cy
Cuts at: 1077 1077
Size: 3938
EcoRI G'AATT_C
Cuts at: 622 942 622
Size: 320 3618
EcoRV GAT'ATC
Cuts at: 399 399
Size: 3938
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1957 1957
Size: 3938
FspI TGC'GCA
Cuts at: 1719 1719
Size: 3938
HaeII r_GCGC'y
Cuts at: 1372 1620 2642 3621 1372
Size: 248 1022 979 1689
Fragments arranged by size:
1689 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1780 1810 1812 1842 1780
Size: 30 2 30 3876
Fragments arranged by size:
3876 30 30 2
HincII GTy'rAC
Cuts at: 977 977
Size: 3938
KasI G'GCGC_C
Cuts at: 1368 1616 1368
Size: 248 3690
KpnI G_GTAC'C
Cuts at: 958 958
Size: 3938
MluI A'CGCG_T
Cuts at: 3371 3371
Size: 3938
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2609 2793 2609
Size: 184 3754
MscI TGG'CCA
Cuts at: 1699 1699
Size: 3938
MslI CAynn'nnrTG
Cuts at: 524 570 638 1927 2054 524
Size: 46 68 1289 127 2408
Fragments arranged by size:
2408 1289 127 68 46
MspA1I CmG'CkG
Cuts at: 1049 1363 1723 2736 2981 1049
Size: 314 360 1013 245 2006
Fragments arranged by size:
2006 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2119 2119
Size: 3938
NarI GG'CG_CC
Cuts at: 1369 1617 1369
Size: 248 3690
NgoMIV G'CCGG_C
Cuts at: 2117 2117
Size: 3938
NspI r_CATG'y
Cuts at: 991 1491 2398 991
Size: 500 907 2531
Fragments arranged by size:
2531 907 500
PciI A'CATG_T
Cuts at: 2394 2394
Size: 3938
PmeI GTTT'AAAC
Cuts at: 937 937
Size: 3938
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3077 3109 3077
Size: 32 3906
PsiI TTA'TAA
Cuts at: 524 700 524
Size: 176 3762
PstI C_TGCA'G
Cuts at: 138 985 1670 138
Size: 847 685 2406
Fragments arranged by size:
2406 847 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3938
PvuII CAG'CTG
Cuts at: 1363 1723 1363
Size: 360 3578
RsrII CG'GwC_CG
Cuts at: 2133 2133
Size: 3938
SacI G_AGCT'C
Cuts at: 131 952 131
Size: 821 3117
SalI G'TCGA_C
Cuts at: 975 975
Size: 3938
SapI GCTCTTCn'nnn_
Cuts at: 912 1961 2171 912
Size: 1049 210 2679
Fragments arranged by size:
2679 1049 210
SbfI CC_TGCA'GG
Cuts at: 985 985
Size: 3938
ScaI AGT'ACT
Cuts at: 3455 3455
Size: 3938
SfcI C'TryA_G
Cuts at: 33 134 204 663 833 981 1666 2659 2850
Size: 101 70 459 170 148 685 993 191
Cuts at: 2850 33
Size: 1121
Fragments arranged by size:
1121 993 685 459 191 170 148 101
70
SfoI GGC'GCC
Cuts at: 1370 1618 1370
Size: 248 3690
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3938
SmaI CCC'GGG
Cuts at: 960 960
Size: 3938
SmlI C'TyrA_G
Cuts at: 1093 2008 2500 2762 1093
Size: 915 492 262 2269
Fragments arranged by size:
2269 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3938
SphI G_CATG'C
Cuts at: 991 1491 991
Size: 500 3438
StyI C'CwwG_G
Cuts at: 1072 1426 1072
Size: 354 3584
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 278 1521 1654 2113 2322 278
Size: 1243 133 459 209 1894
Fragments arranged by size:
1894 1243 459 209 133
TatI w'GTAC_w
Cuts at: 3453 3453
Size: 3938
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3455 3487 3455
Size: 32 3906
Tth111I GACn'n_nGTC
Cuts at: 1735 1735
Size: 3938
XbaI T'CTAG_A
Cuts at: 969 969
Size: 3938
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1433 1433
Size: 3938
XmaI C'CCGG_G
Cuts at: 958 958
Size: 3938
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PmeI PpiI PsiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BcgI BfrBI BglI BmgBI BmtI BsaXI
BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I ClaI
CspCI DraIII FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI