Restriction map for
pF1KE0240
(Circular) (Six-base) MAPSORT of: pF1KE0240.seq Check: 7577 from: 1 to: 4583
pF1KE0240 4583 bp
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AccI GT'mk_AC
Cuts at: 1621 1621
Size: 4583
Acc65I G'GTAC_C
Cuts at: 148 1599 148
Size: 1451 3132
AclI AA'CG_TT
Cuts at: 4423 4423
Size: 4583
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1419 2408 2840 3587 3855 1419
Size: 989 432 747 268 2147
Fragments arranged by size:
2147 989 747 432 268
AfeI AGC'GCT
Cuts at: 1138 1138
Size: 4583
AflIII A'CryG_T
Cuts at: 1935 3039 4016 1935
Size: 1104 977 2502
Fragments arranged by size:
2502 1104 977
AgeI A'CCGG_T
Cuts at: 2971 2971
Size: 4583
AlwNI CAG_nnn'CTG
Cuts at: 547 1237 1888 3455 547
Size: 690 651 1567 1675
Fragments arranged by size:
1675 1567 690 651
ApaI G_GGCC'C
Cuts at: 830 899 1106 830
Size: 69 207 4307
Fragments arranged by size:
4307 207 69
ApaLI G'TGCA_C
Cuts at: 3353 3353
Size: 4583
ApoI r'AATT_y
Cuts at: 214 1587 3936 4192 214
Size: 1373 2349 256 605
Fragments arranged by size:
2349 1373 605 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4583
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4583
AvaI C'yCGr_G
Cuts at: 436 715 1603 436
Size: 279 888 3416
Fragments arranged by size:
3416 888 279
BamHI G'GATC_C
Cuts at: 1608 1608
Size: 4583
BanI G'GyrC_C
Cuts at: 148 769 1599 2013 2261 2296 148
Size: 621 830 414 248 35 2435
Fragments arranged by size:
2435 830 621 414 248 35
BanII G_rGCy'C
Cuts at: 463 606 830 899 1106 1352 1597 2627 463
Size: 143 224 69 207 246 245 1030 2419
Fragments arranged by size:
2419 1030 246 245 224 207 143 69
BbeI G_GCGC'C
Cuts at: 2017 2265 2017
Size: 248 4335
BbsI GAAGACnn'nnnn_
Cuts at: 992 992
Size: 4583
BciVI GTATCCnnnnn_n'
Cuts at: 1329 2481 3242 4583 1329
Size: 1152 761 1341 1329
Fragments arranged by size:
1341 1329 1152 761
BclI T'GATC_A
Cuts at: 2103 2103
Size: 4583
BglI GCCn_nnn'nGGC
Cuts at: 562 562
Size: 4583
BglII A'GATC_T
Cuts at: 2098 4135 2098
Size: 2037 2546
BlpI GC'TnA_GC
Cuts at: 462 1140 1695 462
Size: 678 555 3350
Fragments arranged by size:
3350 678 555
Bme1580I G_kGCm'C
Cuts at: 586 774 809 830 899 1106 1292 2208 2301
Size: 188 35 21 69 207 186 916 93
Cuts at: 2301 3357 586
Size: 1056 1812
Fragments arranged by size:
1812 1056 916 207 188 186 93 69
35 21
BmrI ACTGGGnnnn_n'
Cuts at: 664 796 1391 1795 1876 2211 4355 664
Size: 132 595 404 81 335 2144 892
Fragments arranged by size:
2144 892 595 404 335 132 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2950 2950
Size: 4583
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 322 431 569 582 618 692 979 163
Size: 159 109 138 13 36 74 287 3767
Fragments arranged by size:
3767 287 159 138 109 74 36 13
Bpu10I CC'TnA_GC
Cuts at: 476 476
Size: 4583
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1759 2638 3130 3428 1759
Size: 879 492 298 2914
Fragments arranged by size:
2914 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 298 875 4287 298
Size: 577 3412 594
Fragments arranged by size:
3412 594 577
BsaAI yAC'GTr
Cuts at: 1936 1936
Size: 4583
BsaBI GATnn'nnATC
Cuts at: 6 2097 4134 6
Size: 2091 2037 455
Fragments arranged by size:
2091 2037 455
BsaHI Gr'CG_yC
Cuts at: 2014 2262 2964 4460 2014
Size: 248 702 1496 2137
Fragments arranged by size:
2137 1496 702 248
BsaWI w'CCGG_w
Cuts at: 236 281 1772 2293 2971 3245 3392 3803 236
Size: 45 1491 521 678 274 147 411 1016
Fragments arranged by size:
1491 1016 678 521 411 274 147 45
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 747 777 747
Size: 30 4553
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 457 838 976 457
Size: 381 138 4064
Fragments arranged by size:
4064 381 138
BseYI C'CCAG_C
Cuts at: 571 1046 2008 2766 3343 3839 571
Size: 475 962 758 577 496 1315
Fragments arranged by size:
1315 962 758 577 496 475
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 306 495 306
Size: 189 4394
BsiEI CG_ry'CG
Cuts at: 104 2125 2171 3379 104
Size: 2021 46 1208 1308
Fragments arranged by size:
2021 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 300 1410 1597 2375 2565 3357 300
Size: 1110 187 778 190 792 1526
Fragments arranged by size:
1526 1110 792 778 190 187
Bsp1286I G_dGCh'C
Cuts at: 300 463 586 606 774 809 830 899 1106
Size: 163 123 20 168 35 21 69 207
Cuts at: 1106 1292 1352 1410 1597 2208 2301 2375 2565
Size: 186 60 58 187 611 93 74 190
Cuts at: 2565 2627 3357 300
Size: 62 730 1526
Fragments arranged by size:
1526 730 611 207 190 187 186 168
163 123 93 74 69 62 60 58
35 21 20
BspEI T'CCGG_A
Cuts at: 3803 3803
Size: 4583
BspHI T'CATG_A
Cuts at: 3759 3759
Size: 4583
BspMI ACCTGCnnnn'nnnn_
Cuts at: 919 1290 1633 1847 2149 2530 919
Size: 371 343 214 302 381 2972
Fragments arranged by size:
2972 381 371 343 302 214
BsrBI CCG'CTC
Cuts at: 50 596 632 947 2875 2929 4413 4578 50
Size: 546 36 315 1928 54 1484 165 55
Fragments arranged by size:
1928 1484 546 315 165 55 54 36
BsrDI GCAATG_nn'
Cuts at: 2495 2495
Size: 4583
BsrFI r'CCGG_y
Cuts at: 1069 2581 2762 2971 1069
Size: 1512 181 209 2681
Fragments arranged by size:
2681 1512 209 181
BssSI C'ACGA_G
Cuts at: 2854 3212 2854
Size: 358 4225
BstAPI GCAn_nnn'nTGC
Cuts at: 1256 1960 1256
Size: 704 3879
BstBI TT'CG_AA
Cuts at: 2944 2944
Size: 4583
BstEII G'GTnAC_C
Cuts at: 1038 1419 1038
Size: 381 4202
BstXI CCAn_nnnn'nTGG
Cuts at: 409 747 409
Size: 338 4245
BstYI r'GATC_y
Cuts at: 1 239 1274 1608 2075 2098 2433 2679 3691
Size: 238 1035 334 467 23 335 246 1012
Cuts at: 3691 3777 3789 4135 1
Size: 86 12 346 449
Fragments arranged by size:
1035 1012 467 449 346 335 334 246
238 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1319 1319
Size: 4583
BtgI C'CryG_G
Cuts at: 107 740 1292 107
Size: 633 552 3398
Fragments arranged by size:
3398 633 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2533 2714 2877 2533
Size: 181 163 4239
Fragments arranged by size:
4239 181 163
BtsI GCAGTG_nn'
Cuts at: 1507 1536 1826 1507
Size: 29 290 4264
Fragments arranged by size:
4264 290 29
DraI TTT'AAA
Cuts at: 1582 1582
Size: 4583
DrdI GACnn_nn'nnGTC
Cuts at: 2289 3147 2289
Size: 858 3725
EaeI y'GGCC_r
Cuts at: 839 2168 2342 2733 2760 4105 839
Size: 1329 174 391 27 1345 1317
Fragments arranged by size:
1345 1329 1317 391 174 27
EagI C'GGCC_G
Cuts at: 2168 2168
Size: 4583
EarI CTCTTCn'nnn_
Cuts at: 1438 2606 2816 1438
Size: 1168 210 3205
Fragments arranged by size:
3205 1168 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1112 2791 3101 3247 4393 1112
Size: 1679 310 146 1146 1302
Fragments arranged by size:
1679 1302 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1595 1595
Size: 4583
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 322 431 569 582 618 692 979 1419
Size: 159 109 138 13 36 74 287 440
Cuts at: 1419 2408 2840 3587 3855 163
Size: 989 432 747 268 891
Fragments arranged by size:
989 891 747 440 432 287 268 159
138 109 74 36 13
EcoO109I rG'GnC_Cy
Cuts at: 433 638 698 826 827 895 896 1102 1562
Size: 205 60 128 1 68 1 206 460
Cuts at: 1562 1722 433
Size: 160 3294
Fragments arranged by size:
3294 460 206 205 160 128 68 60
1 1
EcoRI G'AATT_C
Cuts at: 1587 1587
Size: 4583
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 192 2602 192
Size: 2410 2173
FspI TGC'GCA
Cuts at: 2364 2364
Size: 4583
HaeII r_GCGC'y
Cuts at: 1140 2017 2265 3287 4266 1140
Size: 877 248 1022 979 1457
Fragments arranged by size:
1457 1022 979 877 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2425 2455 2457 2487 2425
Size: 30 2 30 4521
Fragments arranged by size:
4521 30 30 2
HincII GTy'rAC
Cuts at: 1622 1622
Size: 4583
KasI G'GCGC_C
Cuts at: 2013 2261 2013
Size: 248 4335
KpnI G_GTAC'C
Cuts at: 152 1603 152
Size: 1451 3132
MluI A'CGCG_T
Cuts at: 4016 4016
Size: 4583
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3254 3438 3254
Size: 184 4399
MscI TGG'CCA
Cuts at: 841 2344 841
Size: 1503 3080
MslI CAynn'nnrTG
Cuts at: 2572 2699 2572
Size: 127 4456
MspA1I CmG'CkG
Cuts at: 957 1144 1270 1694 2008 2368 3381 3626 957
Size: 187 126 424 314 360 1013 245 1914
Fragments arranged by size:
1914 1013 424 360 314 245 187 126
NaeI GCC'GGC
Cuts at: 1071 2764 1071
Size: 1693 2890
NarI GG'CG_CC
Cuts at: 2014 2262 2014
Size: 248 4335
NcoI C'CATG_G
Cuts at: 107 740 1292 107
Size: 633 552 3398
Fragments arranged by size:
3398 633 552
NgoMIV G'CCGG_C
Cuts at: 1069 2762 1069
Size: 1693 2890
NspI r_CATG'y
Cuts at: 1636 2136 3043 1636
Size: 500 907 3176
Fragments arranged by size:
3176 907 500
PciI A'CATG_T
Cuts at: 3039 3039
Size: 4583
PflMI CCAn_nnn'nTGG
Cuts at: 1032 1380 1032
Size: 348 4235
PmeI GTTT'AAAC
Cuts at: 1582 1582
Size: 4583
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 338 370 3722 3754 338
Size: 32 3352 32 1167
Fragments arranged by size:
3352 1167 32 32
PpuMI rG'GwC_Cy
Cuts at: 433 698 1562 433
Size: 265 864 3454
Fragments arranged by size:
3454 864 265
PspOMI G'GGCC_C
Cuts at: 826 895 1102 826
Size: 69 207 4307
Fragments arranged by size:
4307 207 69
PstI C_TGCA'G
Cuts at: 432 1233 1269 1630 2315 432
Size: 801 36 361 685 2700
Fragments arranged by size:
2700 801 685 361 36
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4583
PvuII CAG'CTG
Cuts at: 957 1270 2008 2368 957
Size: 313 738 360 3172
Fragments arranged by size:
3172 738 360 313
RsrII CG'GwC_CG
Cuts at: 2778 2778
Size: 4583
SacI G_AGCT'C
Cuts at: 1597 1597
Size: 4583
SalI G'TCGA_C
Cuts at: 1620 1620
Size: 4583
SapI GCTCTTCn'nnn_
Cuts at: 2606 2816 2606
Size: 210 4373
SbfI CC_TGCA'GG
Cuts at: 1630 1630
Size: 4583
ScaI AGT'ACT
Cuts at: 4100 4100
Size: 4583
SfcI C'TryA_G
Cuts at: 33 428 1229 1265 1626 2311 3304 3495 33
Size: 395 801 36 361 685 993 191 1121
Fragments arranged by size:
1121 993 801 685 395 361 191 36
SfoI GGC'GCC
Cuts at: 2015 2263 2015
Size: 248 4335
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4583
SmaI CCC'GGG
Cuts at: 438 717 1605 438
Size: 279 888 3416
Fragments arranged by size:
3416 888 279
SmlI C'TyrA_G
Cuts at: 1738 2653 3145 3407 1738
Size: 915 492 262 2914
Fragments arranged by size:
2914 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4583
SphI G_CATG'C
Cuts at: 1636 2136 1636
Size: 500 4083
StyI C'CwwG_G
Cuts at: 107 740 1292 1476 1717 2071 107
Size: 633 552 184 241 354 2619
Fragments arranged by size:
2619 633 552 354 241 184
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1143 1405 2166 2299 2758 2967 1143
Size: 262 761 133 459 209 2759
Fragments arranged by size:
2759 761 459 262 209 133
TatI w'GTAC_w
Cuts at: 853 4098 853
Size: 3245 1338
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4100 4132 4100
Size: 32 4551
Tth111I GACn'n_nGTC
Cuts at: 2380 2380
Size: 4583
XbaI T'CTAG_A
Cuts at: 1614 1614
Size: 4583
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2078 2078
Size: 4583
XmaI C'CCGG_G
Cuts at: 436 715 1603 436
Size: 279 888 3416
Fragments arranged by size:
3416 888 279
XmnI GAAnn'nnTTC
Cuts at: 976 976
Size: 4583
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI BsiWI
BsmI BsmBI BsrGI BssHII BstZ17I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PfoI PmlI
PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI