Restriction map for
pF1KE0243
(Circular) (Six-base) MAPSORT of: pF1KE0243.seq Check: 616 from: 1 to: 4277
pF1KE0243 4277 bp
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AccI GT'mk_AC
Cuts at: 422 752 1315 422
Size: 330 563 3384
Fragments arranged by size:
3384 563 330
Acc65I G'GTAC_C
Cuts at: 1293 1293
Size: 4277
AclI AA'CG_TT
Cuts at: 4117 4117
Size: 4277
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 241 253 2102 2534 3281 3549 241
Size: 12 1849 432 747 268 969
Fragments arranged by size:
1849 969 747 432 268 12
AflIII A'CryG_T
Cuts at: 1629 2733 3710 1629
Size: 1104 977 2196
Fragments arranged by size:
2196 1104 977
AgeI A'CCGG_T
Cuts at: 2665 2665
Size: 4277
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 967 967
Size: 4277
AlwNI CAG_nnn'CTG
Cuts at: 309 726 1582 3149 309
Size: 417 856 1567 1437
Fragments arranged by size:
1567 1437 856 417
ApaLI G'TGCA_C
Cuts at: 3047 3047
Size: 4277
ApoI r'AATT_y
Cuts at: 1281 3630 3886 1281
Size: 2349 256 1672
Fragments arranged by size:
2349 1672 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4277
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4277
AvaI C'yCGr_G
Cuts at: 1297 1297
Size: 4277
BamHI G'GATC_C
Cuts at: 1190 1302 1190
Size: 112 4165
BanI G'GyrC_C
Cuts at: 411 520 837 943 1293 1707 1955 1990 411
Size: 109 317 106 350 414 248 35 2698
Fragments arranged by size:
2698 414 350 317 248 109 106 35
BanII G_rGCy'C
Cuts at: 1291 2321 1291
Size: 1030 3247
BbeI G_GCGC'C
Cuts at: 1711 1959 1711
Size: 248 4029
BbsI GAAGACnn'nnnn_
Cuts at: 1062 1197 1221 1062
Size: 135 24 4118
Fragments arranged by size:
4118 135 24
BbvCI CC'TCA_GC
Cuts at: 757 914 757
Size: 157 4120
BciVI GTATCCnnnnn_n'
Cuts at: 541 2175 2936 4277 541
Size: 1634 761 1341 541
Fragments arranged by size:
1634 1341 761 541
BclI T'GATC_A
Cuts at: 1797 1797
Size: 4277
BglI GCCn_nnn'nGGC
Cuts at: 1267 1267
Size: 4277
BglII A'GATC_T
Cuts at: 590 1792 3829 590
Size: 1202 2037 1038
Fragments arranged by size:
2037 1202 1038
BlpI GC'TnA_GC
Cuts at: 1041 1389 1041
Size: 348 3929
Bme1580I G_kGCm'C
Cuts at: 416 1902 1995 3051 416
Size: 1486 93 1056 1642
Fragments arranged by size:
1642 1486 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 267 843 1100 1489 1570 1905 4049 267
Size: 576 257 389 81 335 2144 495
Fragments arranged by size:
2144 576 495 389 335 257 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 522 2644 522
Size: 2122 2155
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 331 414 1199 331
Size: 83 785 3409
Fragments arranged by size:
3409 785 83
Bpu10I CC'TnA_GC
Cuts at: 601 757 914 601
Size: 156 157 3964
Fragments arranged by size:
3964 157 156
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 210 1453 2332 2824 3122 210
Size: 1243 879 492 298 1365
Fragments arranged by size:
1365 1243 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3981 3981
Size: 4277
BsaAI yAC'GTr
Cuts at: 1630 1630
Size: 4277
BsaBI GATnn'nnATC
Cuts at: 6 1791 3828 6
Size: 1785 2037 455
Fragments arranged by size:
2037 1785 455
BsaHI Gr'CG_yC
Cuts at: 1708 1956 2658 4154 1708
Size: 248 702 1496 1831
Fragments arranged by size:
1831 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1466 1987 2665 2939 3086 3497 1466
Size: 521 678 274 147 411 2246
Fragments arranged by size:
2246 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 379 409 379
Size: 30 4247
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 387 672 387
Size: 285 3992
BseYI C'CCAG_C
Cuts at: 126 252 670 1702 2460 3037 3533 126
Size: 126 418 1032 758 577 496 870
Fragments arranged by size:
1032 870 758 577 496 418 126
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 426 426
Size: 4277
BsiEI CG_ry'CG
Cuts at: 104 1819 1865 3073 104
Size: 1715 46 1208 1308
Fragments arranged by size:
1715 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1291 2069 2259 3051 1291
Size: 778 190 792 2517
Fragments arranged by size:
2517 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 601 601
Size: 4277
Bsp1286I G_dGCh'C
Cuts at: 416 1291 1902 1995 2069 2259 2321 3051 416
Size: 875 611 93 74 190 62 730 1642
Fragments arranged by size:
1642 875 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3497 3497
Size: 4277
BspHI T'CATG_A
Cuts at: 3453 3453
Size: 4277
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1327 1541 1843 2224 1327
Size: 214 302 381 3380
Fragments arranged by size:
3380 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2569 2623 4107 4272 50
Size: 2519 54 1484 165 55
Fragments arranged by size:
2519 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 974 2189 974
Size: 1215 3062
BsrFI r'CCGG_y
Cuts at: 391 523 940 958 2275 2456 2665 391
Size: 132 417 18 1317 181 209 2003
Fragments arranged by size:
2003 1317 417 209 181 132 18
BsrGI T'GTAC_A
Cuts at: 158 158
Size: 4277
BssSI C'ACGA_G
Cuts at: 2548 2906 2548
Size: 358 3919
BstAPI GCAn_nnn'nTGC
Cuts at: 1654 1654
Size: 4277
BstBI TT'CG_AA
Cuts at: 2638 2638
Size: 4277
BstYI r'GATC_y
Cuts at: 1 590 1190 1302 1769 1792 2127 2373 3385
Size: 589 600 112 467 23 335 246 1012
Cuts at: 3385 3471 3483 3829 1
Size: 86 12 346 449
Fragments arranged by size:
1012 600 589 467 449 346 335 246
112 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 467 467
Size: 4277
BtgI C'CryG_G
Cuts at: 872 1265 872
Size: 393 3884
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 888 2227 2408 2571 888
Size: 1339 181 163 2594
Fragments arranged by size:
2594 1339 181 163
BtsI GCAGTG_nn'
Cuts at: 1520 1520
Size: 4277
DraI TTT'AAA
Cuts at: 1276 1276
Size: 4277
DraIII CAC_nnn'GTG
Cuts at: 1108 1108
Size: 4277
DrdI GACnn_nn'nnGTC
Cuts at: 1030 1983 2841 1030
Size: 953 858 2466
Fragments arranged by size:
2466 953 858
EaeI y'GGCC_r
Cuts at: 1862 2036 2427 2454 3799 1862
Size: 174 391 27 1345 2340
Fragments arranged by size:
2340 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1862 1862
Size: 4277
EarI CTCTTCn'nnn_
Cuts at: 748 813 2300 2510 748
Size: 65 1487 210 2515
Fragments arranged by size:
2515 1487 210 65
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1201 2485 2795 2941 4087 1201
Size: 1284 310 146 1146 1391
Fragments arranged by size:
1391 1284 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1289 1289
Size: 4277
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 241 253 331 414 1199 2102 2534 3281 3549
Size: 12 78 83 785 903 432 747 268
Cuts at: 3549 241
Size: 969
Fragments arranged by size:
969 903 785 747 432 268 83 78
12
EcoNI CCTnn'n_nnAGG
Cuts at: 220 220
Size: 4277
EcoO109I rG'GnC_Cy
Cuts at: 165 723 738 1416 165
Size: 558 15 678 3026
Fragments arranged by size:
3026 678 558 15
EcoRI G'AATT_C
Cuts at: 1281 1281
Size: 4277
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 204 2296 204
Size: 2092 2185
FspI TGC'GCA
Cuts at: 2058 2058
Size: 4277
HaeII r_GCGC'y
Cuts at: 142 764 1711 1959 2981 3960 142
Size: 622 947 248 1022 979 459
Fragments arranged by size:
1022 979 947 622 459 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 379 411 730 762 887 919 2119 2149 2151
Size: 32 319 32 125 32 1200 30 2
Cuts at: 2151 2181 379
Size: 30 2475
Fragments arranged by size:
2475 1200 319 125 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 936 1316 936
Size: 380 3897
KasI G'GCGC_C
Cuts at: 1707 1955 1707
Size: 248 4029
KpnI G_GTAC'C
Cuts at: 1297 1297
Size: 4277
MluI A'CGCG_T
Cuts at: 3710 3710
Size: 4277
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 425 546 2948 3132 425
Size: 121 2402 184 1570
Fragments arranged by size:
2402 1570 184 121
MscI TGG'CCA
Cuts at: 2038 2038
Size: 4277
MslI CAynn'nnrTG
Cuts at: 886 2266 2393 886
Size: 1380 127 2770
Fragments arranged by size:
2770 1380 127
MspA1I CmG'CkG
Cuts at: 1037 1388 1702 2062 3075 3320 1037
Size: 351 314 360 1013 245 1994
Fragments arranged by size:
1994 1013 360 351 314 245
NaeI GCC'GGC
Cuts at: 2458 2458
Size: 4277
NarI GG'CG_CC
Cuts at: 1708 1956 1708
Size: 248 4029
NgoMIV G'CCGG_C
Cuts at: 2456 2456
Size: 4277
NspI r_CATG'y
Cuts at: 1330 1830 2737 1330
Size: 500 907 2870
Fragments arranged by size:
2870 907 500
PciI A'CATG_T
Cuts at: 2733 2733
Size: 4277
PflMI CCAn_nnn'nTGG
Cuts at: 113 407 726 113
Size: 294 319 3664
Fragments arranged by size:
3664 319 294
PmeI GTTT'AAAC
Cuts at: 1276 1276
Size: 4277
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3416 3448 3416
Size: 32 4245
PpuMI rG'GwC_Cy
Cuts at: 165 738 165
Size: 573 3704
PstI C_TGCA'G
Cuts at: 1124 1324 2009 1124
Size: 200 685 3392
Fragments arranged by size:
3392 685 200
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4277
PvuII CAG'CTG
Cuts at: 1037 1702 2062 1037
Size: 665 360 3252
Fragments arranged by size:
3252 665 360
RsrII CG'GwC_CG
Cuts at: 2472 2472
Size: 4277
SacI G_AGCT'C
Cuts at: 1291 1291
Size: 4277
SalI G'TCGA_C
Cuts at: 1314 1314
Size: 4277
SapI GCTCTTCn'nnn_
Cuts at: 2300 2510 2300
Size: 210 4067
SbfI CC_TGCA'GG
Cuts at: 1324 1324
Size: 4277
ScaI AGT'ACT
Cuts at: 265 334 1219 3794 265
Size: 69 885 2575 748
Fragments arranged by size:
2575 885 748 69
SexAI A'CCwGG_T
Cuts at: 1247 1247
Size: 4277
SfcI C'TryA_G
Cuts at: 33 1120 1320 2005 2998 3189 33
Size: 1087 200 685 993 191 1121
Fragments arranged by size:
1121 1087 993 685 200 191
SfoI GGC'GCC
Cuts at: 1709 1957 1709
Size: 248 4029
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4277
SmaI CCC'GGG
Cuts at: 1299 1299
Size: 4277
SmlI C'TyrA_G
Cuts at: 189 1432 2347 2839 3101 189
Size: 1243 915 492 262 1365
Fragments arranged by size:
1365 1243 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4277
SphI G_CATG'C
Cuts at: 1330 1830 1330
Size: 500 3777
StyI C'CwwG_G
Cuts at: 1411 1765 1411
Size: 354 3923
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 399 825 1119 1186 1860 1993 2452 2661 399
Size: 426 294 67 674 133 459 209 2015
Fragments arranged by size:
2015 674 459 426 294 209 133 67
TatI w'GTAC_w
Cuts at: 158 263 332 1217 3792 158
Size: 105 69 885 2575 643
Fragments arranged by size:
2575 885 643 105 69
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1182 1214 3794 3826 1182
Size: 32 2580 32 1633
Fragments arranged by size:
2580 1633 32 32
Tth111I GACn'n_nGTC
Cuts at: 556 1066 2074 556
Size: 510 1008 2759
Fragments arranged by size:
2759 1008 510
XbaI T'CTAG_A
Cuts at: 1308 1308
Size: 4277
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1772 1772
Size: 4277
XmaI C'CCGG_G
Cuts at: 1297 1297
Size: 4277
XmnI GAAnn'nnTTC
Cuts at: 226 226
Size: 4277
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PflMI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BcgI BfrBI BmgBI BmtI BsiWI
BsmI BssHII BstEII BstXI BstZ17I ClaI CspCI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PfoI PmlI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI ZraI