Restriction map for
pF1KE0244
(Circular) (Six-base) MAPSORT of: pF1KE0244.seq Check: 3872 from: 1 to: 3515
pF1KE0244 3515 bp
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AccI GT'mk_AC
Cuts at: 553 553
Size: 3515
Acc65I G'GTAC_C
Cuts at: 531 531
Size: 3515
AclI AA'CG_TT
Cuts at: 3355 3355
Size: 3515
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1340 1772 2519 2787 1340
Size: 432 747 268 2068
Fragments arranged by size:
2068 747 432 268
AflIII A'CryG_T
Cuts at: 867 1971 2948 867
Size: 1104 977 1434
Fragments arranged by size:
1434 1104 977
AgeI A'CCGG_T
Cuts at: 1903 1903
Size: 3515
AlwNI CAG_nnn'CTG
Cuts at: 820 2387 820
Size: 1567 1948
ApaLI G'TGCA_C
Cuts at: 2285 2285
Size: 3515
ApoI r'AATT_y
Cuts at: 304 519 2868 3124 304
Size: 215 2349 256 695
Fragments arranged by size:
2349 695 256 215
AseI AT'TA_AT
Cuts at: 20 20
Size: 3515
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3515
AvaI C'yCGr_G
Cuts at: 535 535
Size: 3515
BamHI G'GATC_C
Cuts at: 540 540
Size: 3515
BanI G'GyrC_C
Cuts at: 531 945 1193 1228 531
Size: 414 248 35 2818
Fragments arranged by size:
2818 414 248 35
BanII G_rGCy'C
Cuts at: 382 529 1559 382
Size: 147 1030 2338
Fragments arranged by size:
2338 1030 147
BbeI G_GCGC'C
Cuts at: 949 1197 949
Size: 248 3267
BciVI GTATCCnnnnn_n'
Cuts at: 1413 2174 3515 1413
Size: 761 1341 1413
Fragments arranged by size:
1413 1341 761
BclI T'GATC_A
Cuts at: 201 464 1035 201
Size: 263 571 2681
Fragments arranged by size:
2681 571 263
BglII A'GATC_T
Cuts at: 1030 3067 1030
Size: 2037 1478
BlpI GC'TnA_GC
Cuts at: 627 627
Size: 3515
Bme1580I G_kGCm'C
Cuts at: 1140 1233 2289 1140
Size: 93 1056 2366
Fragments arranged by size:
2366 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 727 808 1143 3287 727
Size: 81 335 2144 955
Fragments arranged by size:
2144 955 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1882 1882
Size: 3515
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 394 691 1570 2062 2360 394
Size: 297 879 492 298 1549
Fragments arranged by size:
1549 879 492 298 297
BsaI GGTCTCn'nnnn_
Cuts at: 3219 3219
Size: 3515
BsaAI yAC'GTr
Cuts at: 168 868 168
Size: 700 2815
BsaBI GATnn'nnATC
Cuts at: 6 1029 3066 6
Size: 1023 2037 455
Fragments arranged by size:
2037 1023 455
BsaHI Gr'CG_yC
Cuts at: 946 1194 1896 3392 946
Size: 248 702 1496 1069
Fragments arranged by size:
1496 1069 702 248
BsaWI w'CCGG_w
Cuts at: 704 1225 1903 2177 2324 2735 704
Size: 521 678 274 147 411 1484
Fragments arranged by size:
1484 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 430 460 430
Size: 30 3485
BseYI C'CCAG_C
Cuts at: 384 940 1698 2275 2771 384
Size: 556 758 577 496 1128
Fragments arranged by size:
1128 758 577 556 496
BsiEI CG_ry'CG
Cuts at: 104 1057 1103 2311 104
Size: 953 46 1208 1308
Fragments arranged by size:
1308 1208 953 46
BsiHKAI G_wGCw'C
Cuts at: 529 1307 1497 2289 529
Size: 778 190 792 1755
Fragments arranged by size:
1755 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 382 529 1140 1233 1307 1497 1559 2289 382
Size: 147 611 93 74 190 62 730 1608
Fragments arranged by size:
1608 730 611 190 147 93 74 62
BspEI T'CCGG_A
Cuts at: 2735 2735
Size: 3515
BspHI T'CATG_A
Cuts at: 2691 2691
Size: 3515
BspMI ACCTGCnnnn'nnnn_
Cuts at: 169 565 779 1081 1462 169
Size: 396 214 302 381 2222
Fragments arranged by size:
2222 396 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1807 1861 3345 3510 50
Size: 1757 54 1484 165 55
Fragments arranged by size:
1757 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1427 1427
Size: 3515
BsrFI r'CCGG_y
Cuts at: 1513 1694 1903 1513
Size: 181 209 3125
Fragments arranged by size:
3125 209 181
BsrGI T'GTAC_A
Cuts at: 399 399
Size: 3515
BssSI C'ACGA_G
Cuts at: 1786 2144 1786
Size: 358 3157
BstAPI GCAn_nnn'nTGC
Cuts at: 892 892
Size: 3515
BstBI TT'CG_AA
Cuts at: 132 1876 132
Size: 1744 1771
BstYI r'GATC_y
Cuts at: 1 540 1007 1030 1365 1611 2623 2709 2721
Size: 539 467 23 335 246 1012 86 12
Cuts at: 2721 3067 1
Size: 346 449
Fragments arranged by size:
1012 539 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1465 1646 1809 1465
Size: 181 163 3171
Fragments arranged by size:
3171 181 163
BtsI GCAGTG_nn'
Cuts at: 758 758
Size: 3515
DraI TTT'AAA
Cuts at: 514 514
Size: 3515
DrdI GACnn_nn'nnGTC
Cuts at: 1221 2079 1221
Size: 858 2657
EaeI y'GGCC_r
Cuts at: 1100 1274 1665 1692 3037 1100
Size: 174 391 27 1345 1578
Fragments arranged by size:
1578 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1100 1100
Size: 3515
EarI CTCTTCn'nnn_
Cuts at: 1538 1748 1538
Size: 210 3305
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1723 2033 2179 3325 1723
Size: 310 146 1146 1913
Fragments arranged by size:
1913 1146 310 146
EcoICRI GAG'CTC
Cuts at: 527 527
Size: 3515
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1340 1772 2519 2787 1340
Size: 432 747 268 2068
Fragments arranged by size:
2068 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 654 654
Size: 3515
EcoRI G'AATT_C
Cuts at: 519 519
Size: 3515
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1534 1534
Size: 3515
FspI TGC'GCA
Cuts at: 1296 1296
Size: 3515
HaeII r_GCGC'y
Cuts at: 949 1197 2219 3198 949
Size: 248 1022 979 1266
Fragments arranged by size:
1266 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1357 1387 1389 1419 1357
Size: 30 2 30 3453
Fragments arranged by size:
3453 30 30 2
HincII GTy'rAC
Cuts at: 554 554
Size: 3515
KasI G'GCGC_C
Cuts at: 945 1193 945
Size: 248 3267
KpnI G_GTAC'C
Cuts at: 535 535
Size: 3515
MluI A'CGCG_T
Cuts at: 2948 2948
Size: 3515
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2186 2370 2186
Size: 184 3331
MscI TGG'CCA
Cuts at: 1276 1276
Size: 3515
MslI CAynn'nnrTG
Cuts at: 1504 1631 1504
Size: 127 3388
MspA1I CmG'CkG
Cuts at: 626 940 1300 2313 2558 626
Size: 314 360 1013 245 1583
Fragments arranged by size:
1583 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1696 1696
Size: 3515
NarI GG'CG_CC
Cuts at: 946 1194 946
Size: 248 3267
NgoMIV G'CCGG_C
Cuts at: 1694 1694
Size: 3515
NspI r_CATG'y
Cuts at: 568 1068 1975 568
Size: 500 907 2108
Fragments arranged by size:
2108 907 500
PciI A'CATG_T
Cuts at: 1971 1971
Size: 3515
PmeI GTTT'AAAC
Cuts at: 514 514
Size: 3515
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2654 2686 2654
Size: 32 3483
PshAI GACnn'nnGTC
Cuts at: 256 256
Size: 3515
PstI C_TGCA'G
Cuts at: 562 1247 562
Size: 685 2830
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3515
PvuII CAG'CTG
Cuts at: 940 1300 940
Size: 360 3155
RsrII CG'GwC_CG
Cuts at: 1710 1710
Size: 3515
SacI G_AGCT'C
Cuts at: 529 529
Size: 3515
SalI G'TCGA_C
Cuts at: 552 552
Size: 3515
SapI GCTCTTCn'nnn_
Cuts at: 1538 1748 1538
Size: 210 3305
SbfI CC_TGCA'GG
Cuts at: 562 562
Size: 3515
ScaI AGT'ACT
Cuts at: 3032 3032
Size: 3515
SfcI C'TryA_G
Cuts at: 33 558 1243 2236 2427 33
Size: 525 685 993 191 1121
Fragments arranged by size:
1121 993 685 525 191
SfoI GGC'GCC
Cuts at: 947 1195 947
Size: 248 3267
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3515
SmaI CCC'GGG
Cuts at: 537 537
Size: 3515
SmlI C'TyrA_G
Cuts at: 373 670 1585 2077 2339 373
Size: 297 915 492 262 1549
Fragments arranged by size:
1549 915 492 297 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3515
SphI G_CATG'C
Cuts at: 568 1068 568
Size: 500 3015
SspI AAT'ATT
Cuts at: 318 318
Size: 3515
StyI C'CwwG_G
Cuts at: 449 649 1003 449
Size: 200 354 2961
Fragments arranged by size:
2961 354 200
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1098 1231 1690 1899 1098
Size: 133 459 209 2714
Fragments arranged by size:
2714 459 209 133
TatI w'GTAC_w
Cuts at: 399 3030 399
Size: 2631 884
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 430 462 3032 3064 430
Size: 32 2570 32 881
Fragments arranged by size:
2570 881 32 32
Tth111I GACn'n_nGTC
Cuts at: 1312 1312
Size: 3515
XbaI T'CTAG_A
Cuts at: 505 546 505
Size: 41 3474
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1010 1010
Size: 3515
XmaI C'CCGG_G
Cuts at: 535 535
Size: 3515
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstYI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PmeI PpiI PshAI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI BpmI Bpu10I BseRI BsgI BsiWI
BsmI BsmBI BssHII BstEII BstXI BstZ17I Bsu36I BtgI
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PpuMI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI XmnI ZraI