Restriction map for
pF1KE0250
(Circular) (Six-base) MAPSORT of: pF1KE0250.seq Check: 951 from: 1 to: 3701
pF1KE0250 3701 bp
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AccI GT'mk_AC
Cuts at: 739 739
Size: 3701
Acc65I G'GTAC_C
Cuts at: 717 717
Size: 3701
AclI AA'CG_TT
Cuts at: 3541 3541
Size: 3701
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 712 1526 1958 2705 2973 712
Size: 814 432 747 268 1440
Fragments arranged by size:
1440 814 747 432 268
AflIII A'CryG_T
Cuts at: 1053 2157 3134 1053
Size: 1104 977 1620
Fragments arranged by size:
1620 1104 977
AgeI A'CCGG_T
Cuts at: 206 2089 206
Size: 1883 1818
AlwNI CAG_nnn'CTG
Cuts at: 1006 2573 1006
Size: 1567 2134
ApaLI G'TGCA_C
Cuts at: 2471 2471
Size: 3701
ApoI r'AATT_y
Cuts at: 705 3054 3310 705
Size: 2349 256 1096
Fragments arranged by size:
2349 1096 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3701
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3701
AvaI C'yCGr_G
Cuts at: 721 721
Size: 3701
BamHI G'GATC_C
Cuts at: 686 726 686
Size: 40 3661
BanI G'GyrC_C
Cuts at: 717 1131 1379 1414 717
Size: 414 248 35 3004
Fragments arranged by size:
3004 414 248 35
BanII G_rGCy'C
Cuts at: 715 1745 715
Size: 1030 2671
BbeI G_GCGC'C
Cuts at: 1135 1383 1135
Size: 248 3453
BciVI GTATCCnnnnn_n'
Cuts at: 1599 2360 3701 1599
Size: 761 1341 1599
Fragments arranged by size:
1599 1341 761
BclI T'GATC_A
Cuts at: 1221 1221
Size: 3701
BfrBI ATG'CAT
Cuts at: 463 463
Size: 3701
BglII A'GATC_T
Cuts at: 497 1216 3253 497
Size: 719 2037 945
Fragments arranged by size:
2037 945 719
BlpI GC'TnA_GC
Cuts at: 813 813
Size: 3701
Bme1580I G_kGCm'C
Cuts at: 219 1326 1419 2475 219
Size: 1107 93 1056 1445
Fragments arranged by size:
1445 1107 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 913 994 1329 3473 913
Size: 81 335 2144 1141
Fragments arranged by size:
2144 1141 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2068 2068
Size: 3701
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 244 486 586 244
Size: 242 100 3359
Fragments arranged by size:
3359 242 100
Bpu10I CC'TnA_GC
Cuts at: 244 244
Size: 3701
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 877 1756 2248 2546 877
Size: 879 492 298 2032
Fragments arranged by size:
2032 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 160 290 3405 160
Size: 130 3115 456
Fragments arranged by size:
3115 456 130
BsaAI yAC'GTr
Cuts at: 1054 1054
Size: 3701
BsaBI GATnn'nnATC
Cuts at: 6 1215 3252 6
Size: 1209 2037 455
Fragments arranged by size:
2037 1209 455
BsaHI Gr'CG_yC
Cuts at: 1132 1380 2082 3578 1132
Size: 248 702 1496 1255
Fragments arranged by size:
1496 1255 702 248
BsaWI w'CCGG_w
Cuts at: 206 683 890 1411 2089 2363 2510 2921 206
Size: 477 207 521 678 274 147 411 986
Fragments arranged by size:
986 678 521 477 411 274 207 147
BseYI C'CCAG_C
Cuts at: 1126 1884 2461 2957 1126
Size: 758 577 496 1870
Fragments arranged by size:
1870 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 610 184
Size: 426 3275
BsiEI CG_ry'CG
Cuts at: 104 1243 1289 2497 104
Size: 1139 46 1208 1308
Fragments arranged by size:
1308 1208 1139 46
BsiHKAI G_wGCw'C
Cuts at: 715 1493 1683 2475 715
Size: 778 190 792 1941
Fragments arranged by size:
1941 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 219 715 1326 1419 1493 1683 1745 2475 219
Size: 496 611 93 74 190 62 730 1445
Fragments arranged by size:
1445 730 611 496 190 93 74 62
BspEI T'CCGG_A
Cuts at: 683 2921 683
Size: 2238 1463
BspHI T'CATG_A
Cuts at: 2877 2877
Size: 3701
BspMI ACCTGCnnnn'nnnn_
Cuts at: 510 751 965 1267 1648 510
Size: 241 214 302 381 2563
Fragments arranged by size:
2563 381 302 241 214
BsrBI CCG'CTC
Cuts at: 50 1993 2047 3531 3696 50
Size: 1943 54 1484 165 55
Fragments arranged by size:
1943 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1613 1613
Size: 3701
BsrFI r'CCGG_y
Cuts at: 169 206 1699 1880 2089 169
Size: 37 1493 181 209 1781
Fragments arranged by size:
1781 1493 209 181 37
BssSI C'ACGA_G
Cuts at: 1972 2330 1972
Size: 358 3343
BstAPI GCAn_nnn'nTGC
Cuts at: 513 1078 513
Size: 565 3136
BstBI TT'CG_AA
Cuts at: 2062 2062
Size: 3701
BstYI r'GATC_y
Cuts at: 1 255 497 686 726 1193 1216 1551 1797
Size: 254 242 189 40 467 23 335 246
Cuts at: 1797 2809 2895 2907 3253 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 346 335 254 246 242
189 86 40 23 12
BtgI C'CryG_G
Cuts at: 107 131 107
Size: 24 3677
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 503 558 1651 1832 1995 503
Size: 55 1093 181 163 2209
Fragments arranged by size:
2209 1093 181 163 55
BtsI GCAGTG_nn'
Cuts at: 944 944
Size: 3701
DraI TTT'AAA
Cuts at: 700 700
Size: 3701
DrdI GACnn_nn'nnGTC
Cuts at: 1407 2265 1407
Size: 858 2843
EaeI y'GGCC_r
Cuts at: 1286 1460 1851 1878 3223 1286
Size: 174 391 27 1345 1764
Fragments arranged by size:
1764 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1286 1286
Size: 3701
EarI CTCTTCn'nnn_
Cuts at: 551 1724 1934 551
Size: 1173 210 2318
Fragments arranged by size:
2318 1173 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 184 1909 2219 2365 3511 184
Size: 1725 310 146 1146 374
Fragments arranged by size:
1725 1146 374 310 146
EcoICRI GAG'CTC
Cuts at: 713 713
Size: 3701
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 244 486 586 712 1526 1958 2705 2973 244
Size: 242 100 126 814 432 747 268 972
Fragments arranged by size:
972 814 747 432 268 242 126 100
EcoO109I rG'GnC_Cy
Cuts at: 840 840
Size: 3701
EcoRI G'AATT_C
Cuts at: 705 705
Size: 3701
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1720 1720
Size: 3701
FspI TGC'GCA
Cuts at: 1482 1482
Size: 3701
HaeII r_GCGC'y
Cuts at: 277 1135 1383 2405 3384 277
Size: 858 248 1022 979 594
Fragments arranged by size:
1022 979 858 594 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1543 1573 1575 1605 1543
Size: 30 2 30 3639
Fragments arranged by size:
3639 30 30 2
HincII GTy'rAC
Cuts at: 740 740
Size: 3701
HindIII A'AGCT_T
Cuts at: 312 312
Size: 3701
KasI G'GCGC_C
Cuts at: 1131 1379 1131
Size: 248 3453
KpnI G_GTAC'C
Cuts at: 721 721
Size: 3701
MluI A'CGCG_T
Cuts at: 3134 3134
Size: 3701
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2372 2556 2372
Size: 184 3517
MscI TGG'CCA
Cuts at: 1462 1462
Size: 3701
MslI CAynn'nnrTG
Cuts at: 1690 1817 1690
Size: 127 3574
MspA1I CmG'CkG
Cuts at: 133 564 812 1126 1486 2499 2744 133
Size: 431 248 314 360 1013 245 1090
Fragments arranged by size:
1090 1013 431 360 314 248 245
NaeI GCC'GGC
Cuts at: 1882 1882
Size: 3701
NarI GG'CG_CC
Cuts at: 1132 1380 1132
Size: 248 3453
NcoI C'CATG_G
Cuts at: 107 107
Size: 3701
NgoMIV G'CCGG_C
Cuts at: 1880 1880
Size: 3701
NruI TCG'CGA
Cuts at: 488 488
Size: 3701
NsiI A_TGCA'T
Cuts at: 465 465
Size: 3701
NspI r_CATG'y
Cuts at: 754 1254 2161 754
Size: 500 907 2294
Fragments arranged by size:
2294 907 500
PasI CC'CwG_GG
Cuts at: 291 291
Size: 3701
PciI A'CATG_T
Cuts at: 2157 2157
Size: 3701
PflMI CCAn_nnn'nTGG
Cuts at: 339 339
Size: 3701
PmeI GTTT'AAAC
Cuts at: 700 700
Size: 3701
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2840 2872 2840
Size: 32 3669
PstI C_TGCA'G
Cuts at: 521 748 1433 521
Size: 227 685 2789
Fragments arranged by size:
2789 685 227
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3701
PvuII CAG'CTG
Cuts at: 564 1126 1486 564
Size: 562 360 2779
Fragments arranged by size:
2779 562 360
RsrII CG'GwC_CG
Cuts at: 1896 1896
Size: 3701
SacI G_AGCT'C
Cuts at: 715 715
Size: 3701
SacII CC_GC'GG
Cuts at: 134 134
Size: 3701
SalI G'TCGA_C
Cuts at: 738 738
Size: 3701
SapI GCTCTTCn'nnn_
Cuts at: 551 1724 1934 551
Size: 1173 210 2318
Fragments arranged by size:
2318 1173 210
SbfI CC_TGCA'GG
Cuts at: 748 748
Size: 3701
ScaI AGT'ACT
Cuts at: 3218 3218
Size: 3701
SfcI C'TryA_G
Cuts at: 33 383 517 744 1429 2422 2613 33
Size: 350 134 227 685 993 191 1121
Fragments arranged by size:
1121 993 685 350 227 191 134
SfoI GGC'GCC
Cuts at: 1133 1381 1133
Size: 248 3453
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3701
SmaI CCC'GGG
Cuts at: 723 723
Size: 3701
SmlI C'TyrA_G
Cuts at: 856 1771 2263 2525 856
Size: 915 492 262 2032
Fragments arranged by size:
2032 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3701
SphI G_CATG'C
Cuts at: 754 1254 754
Size: 500 3201
StyI C'CwwG_G
Cuts at: 107 835 1189 107
Size: 728 354 2619
Fragments arranged by size:
2619 728 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 314 1284 1417 1876 2085 314
Size: 970 133 459 209 1930
Fragments arranged by size:
1930 970 459 209 133
TatI w'GTAC_w
Cuts at: 3216 3216
Size: 3701
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3218 3250 3218
Size: 32 3669
Tth111I GACn'n_nGTC
Cuts at: 1498 1498
Size: 3701
XbaI T'CTAG_A
Cuts at: 732 732
Size: 3701
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1196 1196
Size: 3701
XmaI C'CCGG_G
Cuts at: 721 721
Size: 3701
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NruI NsiI NspI
PasI PciI PflMI PmeI PpiI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BglI
BmgBI BmtI BsaXI BseRI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HpaI MfeI NdeI NheI
NotI PacI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI