Restriction map for
pF1KE0254
(Circular) (Six-base) MAPSORT of: pF1KE0254.seq Check: 3209 from: 1 to: 4091
pF1KE0254 4091 bp
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AccI GT'mk_AC
Cuts at: 149 1129 149
Size: 980 3111
Acc65I G'GTAC_C
Cuts at: 1107 1107
Size: 4091
AclI AA'CG_TT
Cuts at: 3931 3931
Size: 4091
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 859 1916 2348 3095 3363 859
Size: 1057 432 747 268 1587
Fragments arranged by size:
1587 1057 747 432 268
AflIII A'CryG_T
Cuts at: 1443 2547 3524 1443
Size: 1104 977 2010
Fragments arranged by size:
2010 1104 977
AgeI A'CCGG_T
Cuts at: 2479 2479
Size: 4091
AlwNI CAG_nnn'CTG
Cuts at: 634 987 1396 2963 634
Size: 353 409 1567 1762
Fragments arranged by size:
1762 1567 409 353
ApaLI G'TGCA_C
Cuts at: 2861 2861
Size: 4091
ApoI r'AATT_y
Cuts at: 118 213 1095 3444 3700 118
Size: 95 882 2349 256 509
Fragments arranged by size:
2349 882 509 256 95
AseI AT'TA_AT
Cuts at: 20 20
Size: 4091
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4091
AvaI C'yCGr_G
Cuts at: 1111 1111
Size: 4091
BamHI G'GATC_C
Cuts at: 1116 1116
Size: 4091
BanI G'GyrC_C
Cuts at: 1107 1521 1769 1804 1107
Size: 414 248 35 3394
Fragments arranged by size:
3394 414 248 35
BanII G_rGCy'C
Cuts at: 914 1030 1105 2135 914
Size: 116 75 1030 2870
Fragments arranged by size:
2870 1030 116 75
BbeI G_GCGC'C
Cuts at: 1525 1773 1525
Size: 248 3843
BciVI GTATCCnnnnn_n'
Cuts at: 1989 2750 4091 1989
Size: 761 1341 1989
Fragments arranged by size:
1989 1341 761
BclI T'GATC_A
Cuts at: 252 1050 1611 252
Size: 798 561 2732
Fragments arranged by size:
2732 798 561
BglII A'GATC_T
Cuts at: 822 1606 3643 822
Size: 784 2037 1270
Fragments arranged by size:
2037 1270 784
BlpI GC'TnA_GC
Cuts at: 1203 1203
Size: 4091
Bme1580I G_kGCm'C
Cuts at: 1716 1809 2865 1716
Size: 93 1056 2942
Fragments arranged by size:
2942 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1303 1384 1719 3863 1303
Size: 81 335 2144 1531
Fragments arranged by size:
2144 1531 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1044 2458 1044
Size: 1414 2677
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 502 1009 502
Size: 507 3584
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 952 1267 2146 2638 2936 952
Size: 315 879 492 298 2107
Fragments arranged by size:
2107 879 492 315 298
BsaI GGTCTCn'nnnn_
Cuts at: 3795 3795
Size: 4091
BsaAI yAC'GTr
Cuts at: 1444 1444
Size: 4091
BsaBI GATnn'nnATC
Cuts at: 6 1605 3642 6
Size: 1599 2037 455
Fragments arranged by size:
2037 1599 455
BsaHI Gr'CG_yC
Cuts at: 1522 1770 2472 3968 1522
Size: 248 702 1496 1645
Fragments arranged by size:
1645 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1280 1801 2479 2753 2900 3311 1280
Size: 521 678 274 147 411 2060
Fragments arranged by size:
2060 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 590 620 716 746 590
Size: 30 96 30 3935
Fragments arranged by size:
3935 96 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 505 505
Size: 4091
BseYI C'CCAG_C
Cuts at: 1516 2274 2851 3347 1516
Size: 758 577 496 2260
Fragments arranged by size:
2260 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1633 1679 2887 104
Size: 1529 46 1208 1308
Fragments arranged by size:
1529 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 968 1030 1105 1883 2073 2865 968
Size: 62 75 778 190 792 2194
Fragments arranged by size:
2194 792 778 190 75 62
BsmI GAATG_Cn'
Cuts at: 366 366
Size: 4091
Bsp1286I G_dGCh'C
Cuts at: 914 968 1030 1105 1716 1809 1883 2073 2135
Size: 54 62 75 611 93 74 190 62
Cuts at: 2135 2865 914
Size: 730 2140
Fragments arranged by size:
2140 730 611 190 93 75 74 62
62 54
BspEI T'CCGG_A
Cuts at: 3311 3311
Size: 4091
BspHI T'CATG_A
Cuts at: 707 3267 707
Size: 2560 1531
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1141 1355 1657 2038 1141
Size: 214 302 381 3194
Fragments arranged by size:
3194 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2383 2437 3921 4086 50
Size: 2333 54 1484 165 55
Fragments arranged by size:
2333 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 404 1068 2003 404
Size: 664 935 2492
Fragments arranged by size:
2492 935 664
BsrFI r'CCGG_y
Cuts at: 2089 2270 2479 2089
Size: 181 209 3701
Fragments arranged by size:
3701 209 181
BssSI C'ACGA_G
Cuts at: 2362 2720 2362
Size: 358 3733
BstAPI GCAn_nnn'nTGC
Cuts at: 634 1468 634
Size: 834 3257
BstBI TT'CG_AA
Cuts at: 2452 2452
Size: 4091
BstXI CCAn_nnnn'nTGG
Cuts at: 135 399 135
Size: 264 3827
BstYI r'GATC_y
Cuts at: 1 350 759 822 1116 1583 1606 1941 2187
Size: 349 409 63 294 467 23 335 246
Cuts at: 2187 3199 3285 3297 3643 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 409 349 346 335 294
246 86 63 23 12
BtgI C'CryG_G
Cuts at: 727 727
Size: 4091
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2041 2222 2385 2041
Size: 181 163 3747
Fragments arranged by size:
3747 181 163
BtsI GCAGTG_nn'
Cuts at: 1334 1334
Size: 4091
DraI TTT'AAA
Cuts at: 690 1090 690
Size: 400 3691
DrdI GACnn_nn'nnGTC
Cuts at: 1797 2655 1797
Size: 858 3233
EaeI y'GGCC_r
Cuts at: 389 1676 1850 2241 2268 3613 389
Size: 1287 174 391 27 1345 867
Fragments arranged by size:
1345 1287 867 391 174 27
EagI C'GGCC_G
Cuts at: 1676 1676
Size: 4091
EarI CTCTTCn'nnn_
Cuts at: 214 259 2114 2324 214
Size: 45 1855 210 1981
Fragments arranged by size:
1981 1855 210 45
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2299 2609 2755 3901 2299
Size: 310 146 1146 2489
Fragments arranged by size:
2489 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1028 1103 1028
Size: 75 4016
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 502 859 1009 1916 2348 3095 3363 502
Size: 357 150 907 432 747 268 1230
Fragments arranged by size:
1230 907 747 432 357 268 150
EcoO109I rG'GnC_Cy
Cuts at: 1230 1230
Size: 4091
EcoRI G'AATT_C
Cuts at: 1095 1095
Size: 4091
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2110 2110
Size: 4091
FspI TGC'GCA
Cuts at: 1872 1872
Size: 4091
HaeII r_GCGC'y
Cuts at: 1525 1773 2795 3774 1525
Size: 248 1022 979 1842
Fragments arranged by size:
1842 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 814 846 1933 1963 1965 1995 814
Size: 32 1087 30 2 30 2910
Fragments arranged by size:
2910 1087 32 30 30 2
HincII GTy'rAC
Cuts at: 1130 1130
Size: 4091
KasI G'GCGC_C
Cuts at: 1521 1769 1521
Size: 248 3843
KpnI G_GTAC'C
Cuts at: 1111 1111
Size: 4091
MluI A'CGCG_T
Cuts at: 3524 3524
Size: 4091
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 555 2762 2946 555
Size: 2207 184 1700
Fragments arranged by size:
2207 1700 184
MscI TGG'CCA
Cuts at: 391 1852 391
Size: 1461 2630
MslI CAynn'nnrTG
Cuts at: 2080 2207 2080
Size: 127 3964
MspA1I CmG'CkG
Cuts at: 1048 1202 1516 1876 2889 3134 1048
Size: 154 314 360 1013 245 2005
Fragments arranged by size:
2005 1013 360 314 245 154
NaeI GCC'GGC
Cuts at: 2272 2272
Size: 4091
NarI GG'CG_CC
Cuts at: 1522 1770 1522
Size: 248 3843
NgoMIV G'CCGG_C
Cuts at: 2270 2270
Size: 4091
NspI r_CATG'y
Cuts at: 156 1144 1644 2551 156
Size: 988 500 907 1696
Fragments arranged by size:
1696 988 907 500
PciI A'CATG_T
Cuts at: 2547 2547
Size: 4091
PmeI GTTT'AAAC
Cuts at: 1090 1090
Size: 4091
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3230 3262 3230
Size: 32 4059
PsiI TTA'TAA
Cuts at: 337 337
Size: 4091
PstI C_TGCA'G
Cuts at: 359 1138 1823 359
Size: 779 685 2627
Fragments arranged by size:
2627 779 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4091
PvuII CAG'CTG
Cuts at: 1048 1516 1876 1048
Size: 468 360 3263
Fragments arranged by size:
3263 468 360
RsrII CG'GwC_CG
Cuts at: 2286 2286
Size: 4091
SacI G_AGCT'C
Cuts at: 1030 1105 1030
Size: 75 4016
SalI G'TCGA_C
Cuts at: 1128 1128
Size: 4091
SapI GCTCTTCn'nnn_
Cuts at: 2114 2324 2114
Size: 210 3881
SbfI CC_TGCA'GG
Cuts at: 1138 1138
Size: 4091
ScaI AGT'ACT
Cuts at: 3608 3608
Size: 4091
SfcI C'TryA_G
Cuts at: 33 167 355 1134 1819 2812 3003 33
Size: 134 188 779 685 993 191 1121
Fragments arranged by size:
1121 993 779 685 191 188 134
SfoI GGC'GCC
Cuts at: 1523 1771 1523
Size: 248 3843
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4091
SmaI CCC'GGG
Cuts at: 1113 1113
Size: 4091
SmlI C'TyrA_G
Cuts at: 967 1246 2161 2653 2915 967
Size: 279 915 492 262 2143
Fragments arranged by size:
2143 915 492 279 262
SpeI A'CTAG_T
Cuts at: 67 927 67
Size: 860 3231
SphI G_CATG'C
Cuts at: 1144 1644 1144
Size: 500 3591
SspI AAT'ATT
Cuts at: 809 809
Size: 4091
StyI C'CwwG_G
Cuts at: 1225 1579 1225
Size: 354 3737
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1674 1807 2266 2475 1674
Size: 133 459 209 3290
Fragments arranged by size:
3290 459 209 133
TatI w'GTAC_w
Cuts at: 3606 3606
Size: 4091
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 716 748 3608 3640 716
Size: 32 2860 32 1167
Fragments arranged by size:
2860 1167 32 32
Tth111I GACn'n_nGTC
Cuts at: 1888 1888
Size: 4091
XbaI T'CTAG_A
Cuts at: 1122 1122
Size: 4091
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1586 1586
Size: 4091
XmaI C'CCGG_G
Cuts at: 1111 1111
Size: 4091
XmnI GAAnn'nnTTC
Cuts at: 499 499
Size: 4091
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PmeI PpiI
PsiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI Bpu10I BsgI BsiWI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI
ZraI