Restriction map for
pF1KE0257
(Circular) (Six-base) MAPSORT of: pF1KE0257.seq Check: 6424 from: 1 to: 4310
pF1KE0257 4310 bp
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AccI GT'mk_AC
Cuts at: 671 812 1013 1348 671
Size: 141 201 335 3633
Fragments arranged by size:
3633 335 201 141
Acc65I G'GTAC_C
Cuts at: 1326 1326
Size: 4310
AclI AA'CG_TT
Cuts at: 4150 4150
Size: 4310
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 613 2135 2567 3314 3582 613
Size: 1522 432 747 268 1341
Fragments arranged by size:
1522 1341 747 432 268
AfeI AGC'GCT
Cuts at: 534 534
Size: 4310
AflIII A'CryG_T
Cuts at: 1662 2766 3743 1662
Size: 1104 977 2229
Fragments arranged by size:
2229 1104 977
AgeI A'CCGG_T
Cuts at: 2698 2698
Size: 4310
AlwNI CAG_nnn'CTG
Cuts at: 1615 3182 1615
Size: 1567 2743
ApaLI G'TGCA_C
Cuts at: 3080 3080
Size: 4310
ApoI r'AATT_y
Cuts at: 1314 3663 3919 1314
Size: 2349 256 1705
Fragments arranged by size:
2349 1705 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4310
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4310
AvaI C'yCGr_G
Cuts at: 1052 1221 1330 1052
Size: 169 109 4032
Fragments arranged by size:
4032 169 109
BamHI G'GATC_C
Cuts at: 1335 1335
Size: 4310
BanI G'GyrC_C
Cuts at: 1326 1740 1988 2023 1326
Size: 414 248 35 3613
Fragments arranged by size:
3613 414 248 35
BanII G_rGCy'C
Cuts at: 1324 2354 1324
Size: 1030 3280
BbeI G_GCGC'C
Cuts at: 1744 1992 1744
Size: 248 4062
BbsI GAAGACnn'nnnn_
Cuts at: 601 601
Size: 4310
BciVI GTATCCnnnnn_n'
Cuts at: 2208 2969 4310 2208
Size: 761 1341 2208
Fragments arranged by size:
2208 1341 761
BclI T'GATC_A
Cuts at: 275 1830 275
Size: 1555 2755
BfrBI ATG'CAT
Cuts at: 1029 1029
Size: 4310
BglII A'GATC_T
Cuts at: 384 1825 3862 384
Size: 1441 2037 832
Fragments arranged by size:
2037 1441 832
BlpI GC'TnA_GC
Cuts at: 1105 1422 1105
Size: 317 3993
Bme1580I G_kGCm'C
Cuts at: 1228 1935 2028 3084 1228
Size: 707 93 1056 2454
Fragments arranged by size:
2454 1056 707 93
BmrI ACTGGGnnnn_n'
Cuts at: 1238 1280 1522 1603 1938 4082 1238
Size: 42 242 81 335 2144 1466
Fragments arranged by size:
2144 1466 335 242 81 42
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2677 2677
Size: 4310
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 215 215
Size: 4310
Bpu10I CC'TnA_GC
Cuts at: 1166 1166
Size: 4310
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 586 1486 2365 2857 3155 586
Size: 900 879 492 298 1741
Fragments arranged by size:
1741 900 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4014 4014
Size: 4310
BsaAI yAC'GTr
Cuts at: 1663 1663
Size: 4310
BsaBI GATnn'nnATC
Cuts at: 6 1824 3861 6
Size: 1818 2037 455
Fragments arranged by size:
2037 1818 455
BsaHI Gr'CG_yC
Cuts at: 1741 1989 2691 4187 1741
Size: 248 702 1496 1864
Fragments arranged by size:
1864 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1499 2020 2698 2972 3119 3530 1499
Size: 521 678 274 147 411 2279
Fragments arranged by size:
2279 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1735 2493 3070 3566 1735
Size: 758 577 496 2479
Fragments arranged by size:
2479 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1852 1898 3106 104
Size: 1748 46 1208 1308
Fragments arranged by size:
1748 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 476 1324 2102 2292 3084 476
Size: 848 778 190 792 1702
Fragments arranged by size:
1702 848 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 476 1228 1324 1935 2028 2102 2292 2354 3084
Size: 752 96 611 93 74 190 62 730
Cuts at: 3084 476
Size: 1702
Fragments arranged by size:
1702 752 730 611 190 96 93 74
62
BspEI T'CCGG_A
Cuts at: 3530 3530
Size: 4310
BspHI T'CATG_A
Cuts at: 3486 3486
Size: 4310
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1360 1574 1876 2257 1360
Size: 214 302 381 3413
Fragments arranged by size:
3413 381 302 214
BsrBI CCG'CTC
Cuts at: 50 218 1161 2602 2656 4140 4305 50
Size: 168 943 1441 54 1484 165 55
Fragments arranged by size:
1484 1441 943 168 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2222 2222
Size: 4310
BsrFI r'CCGG_y
Cuts at: 2308 2489 2698 2308
Size: 181 209 3920
Fragments arranged by size:
3920 209 181
BssSI C'ACGA_G
Cuts at: 2581 2939 2581
Size: 358 3952
BstAPI GCAn_nnn'nTGC
Cuts at: 250 1076 1687 250
Size: 826 611 2873
Fragments arranged by size:
2873 826 611
BstBI TT'CG_AA
Cuts at: 2671 2671
Size: 4310
BstYI r'GATC_y
Cuts at: 1 384 684 1335 1802 1825 2160 2406 3418
Size: 383 300 651 467 23 335 246 1012
Cuts at: 3418 3504 3516 3862 1
Size: 86 12 346 449
Fragments arranged by size:
1012 651 467 449 383 346 335 300
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 672 672
Size: 4310
Bsu36I CC'TnA_GG
Cuts at: 650 650
Size: 4310
BtgI C'CryG_G
Cuts at: 107 107
Size: 4310
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2260 2441 2604 2260
Size: 181 163 3966
Fragments arranged by size:
3966 181 163
BtsI GCAGTG_nn'
Cuts at: 1553 1553
Size: 4310
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 164 199 164
Size: 35 4275
DraI TTT'AAA
Cuts at: 1131 1309 1131
Size: 178 4132
DraIII CAC_nnn'GTG
Cuts at: 1274 1274
Size: 4310
DrdI GACnn_nn'nnGTC
Cuts at: 2016 2874 2016
Size: 858 3452
EaeI y'GGCC_r
Cuts at: 1895 2069 2460 2487 3832 1895
Size: 174 391 27 1345 2373
Fragments arranged by size:
2373 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1895 1895
Size: 4310
EarI CTCTTCn'nnn_
Cuts at: 2333 2543 2333
Size: 210 4100
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1171 2518 2828 2974 4120 1171
Size: 1347 310 146 1146 1361
Fragments arranged by size:
1361 1347 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1322 1322
Size: 4310
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 215 613 2135 2567 3314 3582 215
Size: 398 1522 432 747 268 943
Fragments arranged by size:
1522 943 747 432 398 268
EcoO109I rG'GnC_Cy
Cuts at: 833 1449 833
Size: 616 3694
EcoRI G'AATT_C
Cuts at: 1314 1314
Size: 4310
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 759 2329 759
Size: 1570 2740
FspI TGC'GCA
Cuts at: 2091 2091
Size: 4310
HaeII r_GCGC'y
Cuts at: 536 1744 1992 3014 3993 536
Size: 1208 248 1022 979 853
Fragments arranged by size:
1208 1022 979 853 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 154 186 627 659 2152 2182 2184 2214 154
Size: 32 441 32 1493 30 2 30 2250
Fragments arranged by size:
2250 1493 441 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1349 1349
Size: 4310
HindIII A'AGCT_T
Cuts at: 1113 1113
Size: 4310
KasI G'GCGC_C
Cuts at: 1740 1988 1740
Size: 248 4062
KpnI G_GTAC'C
Cuts at: 1330 1330
Size: 4310
MluI A'CGCG_T
Cuts at: 3743 3743
Size: 4310
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2981 3165 2981
Size: 184 4126
MscI TGG'CCA
Cuts at: 2071 2071
Size: 4310
MslI CAynn'nnrTG
Cuts at: 283 1240 2299 2426 283
Size: 957 1059 127 2167
Fragments arranged by size:
2167 1059 957 127
MspA1I CmG'CkG
Cuts at: 1421 1735 2095 3108 3353 1421
Size: 314 360 1013 245 2378
Fragments arranged by size:
2378 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2491 2491
Size: 4310
NarI GG'CG_CC
Cuts at: 1741 1989 1741
Size: 248 4062
NcoI C'CATG_G
Cuts at: 107 107
Size: 4310
NgoMIV G'CCGG_C
Cuts at: 2489 2489
Size: 4310
NsiI A_TGCA'T
Cuts at: 1031 1031
Size: 4310
NspI r_CATG'y
Cuts at: 1363 1863 2770 1363
Size: 500 907 2903
Fragments arranged by size:
2903 907 500
PciI A'CATG_T
Cuts at: 2766 2766
Size: 4310
PfoI T'CCnGG_A
Cuts at: 231 231
Size: 4310
PmeI GTTT'AAAC
Cuts at: 1309 1309
Size: 4310
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3449 3481 3449
Size: 32 4278
PpuMI rG'GwC_Cy
Cuts at: 833 833
Size: 4310
PstI C_TGCA'G
Cuts at: 1357 2042 1357
Size: 685 3625
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4310
PvuII CAG'CTG
Cuts at: 1735 2095 1735
Size: 360 3950
RsrII CG'GwC_CG
Cuts at: 2505 2505
Size: 4310
SacI G_AGCT'C
Cuts at: 1324 1324
Size: 4310
SalI G'TCGA_C
Cuts at: 1347 1347
Size: 4310
SapI GCTCTTCn'nnn_
Cuts at: 2333 2543 2333
Size: 210 4100
SbfI CC_TGCA'GG
Cuts at: 1357 1357
Size: 4310
ScaI AGT'ACT
Cuts at: 3827 3827
Size: 4310
SfcI C'TryA_G
Cuts at: 33 1353 2038 3031 3222 33
Size: 1320 685 993 191 1121
Fragments arranged by size:
1320 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1742 1990 1742
Size: 248 4062
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4310
SmaI CCC'GGG
Cuts at: 1223 1332 1223
Size: 109 4201
SmlI C'TyrA_G
Cuts at: 565 1465 2380 2872 3134 565
Size: 900 915 492 262 1741
Fragments arranged by size:
1741 915 900 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4310
SphI G_CATG'C
Cuts at: 1363 1863 1363
Size: 500 3810
SspI AAT'ATT
Cuts at: 357 357
Size: 4310
StyI C'CwwG_G
Cuts at: 107 294 1444 1798 107
Size: 187 1150 354 2619
Fragments arranged by size:
2619 1150 354 187
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1262 1893 2026 2485 2694 1262
Size: 631 133 459 209 2878
Fragments arranged by size:
2878 631 459 209 133
TatI w'GTAC_w
Cuts at: 3825 3825
Size: 4310
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3827 3859 3827
Size: 32 4278
Tth111I GACn'n_nGTC
Cuts at: 2107 2107
Size: 4310
XbaI T'CTAG_A
Cuts at: 1341 1341
Size: 4310
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 924 1805 924
Size: 881 3429
XmaI C'CCGG_G
Cuts at: 1221 1330 1221
Size: 109 4201
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PciI PfoI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BglI BmgBI BmtI
BsaXI BseRI BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI ClaI EcoNI EcoRV FseI FspAI HpaI
MfeI NdeI NheI NotI NruI PacI PasI PflMI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI
XmnI ZraI