Restriction map for
pF1KE0261
(Circular) (Six-base) MAPSORT of: pF1KE0261.seq Check: 6334 from: 1 to: 4238
pF1KE0261 4238 bp
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AccI GT'mk_AC
Cuts at: 1276 1276
Size: 4238
Acc65I G'GTAC_C
Cuts at: 1254 1254
Size: 4238
AclI AA'CG_TT
Cuts at: 4078 4078
Size: 4238
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 455 2063 2495 3242 3510 277
Size: 178 1608 432 747 268 1005
Fragments arranged by size:
1608 1005 747 432 268 178
AflIII A'CryG_T
Cuts at: 1590 2694 3671 1590
Size: 1104 977 2157
Fragments arranged by size:
2157 1104 977
AgeI A'CCGG_T
Cuts at: 2626 2626
Size: 4238
AlwNI CAG_nnn'CTG
Cuts at: 781 1543 3110 781
Size: 762 1567 1909
Fragments arranged by size:
1909 1567 762
ApaI G_GGCC'C
Cuts at: 699 699
Size: 4238
ApaLI G'TGCA_C
Cuts at: 3008 3008
Size: 4238
ApoI r'AATT_y
Cuts at: 1242 3591 3847 1242
Size: 2349 256 1633
Fragments arranged by size:
2349 1633 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4238
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4238
AvaI C'yCGr_G
Cuts at: 1258 1258
Size: 4238
BamHI G'GATC_C
Cuts at: 1263 1263
Size: 4238
BanI G'GyrC_C
Cuts at: 1010 1254 1668 1916 1951 1010
Size: 244 414 248 35 3297
Fragments arranged by size:
3297 414 248 244 35
BanII G_rGCy'C
Cuts at: 326 699 1252 2282 326
Size: 373 553 1030 2282
Fragments arranged by size:
2282 1030 553 373
BbeI G_GCGC'C
Cuts at: 1672 1920 1672
Size: 248 3990
BbvCI CC'TCA_GC
Cuts at: 167 167
Size: 4238
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 192 226 192
Size: 34 4204
BciVI GTATCCnnnnn_n'
Cuts at: 2136 2897 4238 2136
Size: 761 1341 2136
Fragments arranged by size:
2136 1341 761
BclI T'GATC_A
Cuts at: 1758 1758
Size: 4238
BglI GCCn_nnn'nGGC
Cuts at: 667 667
Size: 4238
BglII A'GATC_T
Cuts at: 1753 3790 1753
Size: 2037 2201
BlpI GC'TnA_GC
Cuts at: 821 1350 821
Size: 529 3709
Bme1580I G_kGCm'C
Cuts at: 699 1863 1956 3012 699
Size: 1164 93 1056 1925
Fragments arranged by size:
1925 1164 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 371 1450 1531 1866 4010 371
Size: 1079 81 335 2144 599
Fragments arranged by size:
2144 1079 599 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2605 2605
Size: 4238
Bpu10I CC'TnA_GC
Cuts at: 167 167
Size: 4238
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 182 1414 2293 2785 3083 182
Size: 1232 879 492 298 1337
Fragments arranged by size:
1337 1232 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 394 882 3942 394
Size: 488 3060 690
Fragments arranged by size:
3060 690 488
BsaAI yAC'GTr
Cuts at: 1591 1591
Size: 4238
BsaBI GATnn'nnATC
Cuts at: 6 1752 3789 6
Size: 1746 2037 455
Fragments arranged by size:
2037 1746 455
BsaHI Gr'CG_yC
Cuts at: 1669 1917 2619 4115 1669
Size: 248 702 1496 1792
Fragments arranged by size:
1792 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1427 1948 2626 2900 3047 3458 1427
Size: 521 678 274 147 411 2207
Fragments arranged by size:
2207 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 184 775 1036 1102 1165 1168 184
Size: 591 261 66 63 3 3254
Fragments arranged by size:
3254 591 261 66 63 3
BseYI C'CCAG_C
Cuts at: 127 1663 2421 2998 3494 127
Size: 1536 758 577 496 871
Fragments arranged by size:
1536 871 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 216 1780 1826 3034 104
Size: 112 1564 46 1208 1308
Fragments arranged by size:
1564 1308 1208 112 46
BsiHKAI G_wGCw'C
Cuts at: 326 1252 2030 2220 3012 326
Size: 926 778 190 792 1552
Fragments arranged by size:
1552 926 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 679 679
Size: 4238
Bsp1286I G_dGCh'C
Cuts at: 326 699 1252 1863 1956 2030 2220 2282 3012
Size: 373 553 611 93 74 190 62 730
Cuts at: 3012 326
Size: 1552
Fragments arranged by size:
1552 730 611 553 373 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3458 3458
Size: 4238
BspHI T'CATG_A
Cuts at: 3414 3414
Size: 4238
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1221 1288 1502 1804 2185 1221
Size: 67 214 302 381 3274
Fragments arranged by size:
3274 381 302 214 67
BsrBI CCG'CTC
Cuts at: 50 122 2530 2584 4068 4233 50
Size: 72 2408 54 1484 165 55
Fragments arranged by size:
2408 1484 165 72 55 54
BsrDI GCAATG_nn'
Cuts at: 138 2150 138
Size: 2012 2226
BsrFI r'CCGG_y
Cuts at: 2236 2417 2626 2236
Size: 181 209 3848
Fragments arranged by size:
3848 209 181
BssSI C'ACGA_G
Cuts at: 2509 2867 2509
Size: 358 3880
BstAPI GCAn_nnn'nTGC
Cuts at: 1227 1615 1227
Size: 388 3850
BstBI TT'CG_AA
Cuts at: 2599 2599
Size: 4238
BstXI CCAn_nnnn'nTGG
Cuts at: 691 691
Size: 4238
BstYI r'GATC_y
Cuts at: 1 1263 1730 1753 2088 2334 3346 3432 3444
Size: 1262 467 23 335 246 1012 86 12
Cuts at: 3444 3790 1
Size: 346 449
Fragments arranged by size:
1262 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 982 982
Size: 4238
BtgI C'CryG_G
Cuts at: 115 115
Size: 4238
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2188 2369 2532 2188
Size: 181 163 3894
Fragments arranged by size:
3894 181 163
BtsI GCAGTG_nn'
Cuts at: 516 1080 1481 516
Size: 564 401 3273
Fragments arranged by size:
3273 564 401
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 132 167 132
Size: 35 4203
DraI TTT'AAA
Cuts at: 1237 1237
Size: 4238
DrdI GACnn_nn'nnGTC
Cuts at: 1944 2802 1944
Size: 858 3380
EaeI y'GGCC_r
Cuts at: 213 341 1823 1997 2388 2415 3760 213
Size: 128 1482 174 391 27 1345 691
Fragments arranged by size:
1482 1345 691 391 174 128 27
EagI C'GGCC_G
Cuts at: 213 1823 213
Size: 1610 2628
EarI CTCTTCn'nnn_
Cuts at: 331 997 1013 1129 2261 2471 331
Size: 666 16 116 1132 210 2098
Fragments arranged by size:
2098 1132 666 210 116 16
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2446 2756 2902 4048 2446
Size: 310 146 1146 2636
Fragments arranged by size:
2636 1146 310 146
EcoICRI GAG'CTC
Cuts at: 324 1250 324
Size: 926 3312
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 455 2063 2495 3242 3510 277
Size: 178 1608 432 747 268 1005
Fragments arranged by size:
1608 1005 747 432 268 178
EcoNI CCTnn'n_nnAGG
Cuts at: 394 394
Size: 4238
EcoO109I rG'GnC_Cy
Cuts at: 687 695 696 1377 687
Size: 8 1 681 3548
Fragments arranged by size:
3548 681 8 1
EcoRI G'AATT_C
Cuts at: 1242 1242
Size: 4238
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 447 2257 447
Size: 1810 2428
FspI TGC'GCA
Cuts at: 2019 2019
Size: 4238
HaeII r_GCGC'y
Cuts at: 1672 1920 2942 3921 1672
Size: 248 1022 979 1989
Fragments arranged by size:
1989 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2080 2110 2112 2142 2080
Size: 30 2 30 4176
Fragments arranged by size:
4176 30 30 2
HincII GTy'rAC
Cuts at: 1277 1277
Size: 4238
HindIII A'AGCT_T
Cuts at: 631 631
Size: 4238
KasI G'GCGC_C
Cuts at: 1668 1916 1668
Size: 248 3990
KpnI G_GTAC'C
Cuts at: 1258 1258
Size: 4238
MluI A'CGCG_T
Cuts at: 3671 3671
Size: 4238
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2909 3093 2909
Size: 184 4054
MscI TGG'CCA
Cuts at: 1999 1999
Size: 4238
MslI CAynn'nnrTG
Cuts at: 2227 2354 2227
Size: 127 4111
MspA1I CmG'CkG
Cuts at: 117 177 1349 1663 2023 3036 3281 117
Size: 60 1172 314 360 1013 245 1074
Fragments arranged by size:
1172 1074 1013 360 314 245 60
NaeI GCC'GGC
Cuts at: 2419 2419
Size: 4238
NarI GG'CG_CC
Cuts at: 1669 1917 1669
Size: 248 3990
NdeI CA'TA_TG
Cuts at: 1107 1107
Size: 4238
NgoMIV G'CCGG_C
Cuts at: 2417 2417
Size: 4238
NspI r_CATG'y
Cuts at: 820 1230 1291 1791 2698 820
Size: 410 61 500 907 2360
Fragments arranged by size:
2360 907 500 410 61
PasI CC'CwG_GG
Cuts at: 691 691
Size: 4238
PciI A'CATG_T
Cuts at: 2694 2694
Size: 4238
PmeI GTTT'AAAC
Cuts at: 1237 1237
Size: 4238
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3377 3409 3377
Size: 32 4206
PpuMI rG'GwC_Cy
Cuts at: 687 687
Size: 4238
PsiI TTA'TAA
Cuts at: 413 413
Size: 4238
PspOMI G'GGCC_C
Cuts at: 695 695
Size: 4238
PstI C_TGCA'G
Cuts at: 511 710 930 1071 1285 1970 511
Size: 199 220 141 214 685 2779
Fragments arranged by size:
2779 685 220 214 199 141
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4238
PvuII CAG'CTG
Cuts at: 177 1663 2023 177
Size: 1486 360 2392
Fragments arranged by size:
2392 1486 360
RsrII CG'GwC_CG
Cuts at: 2433 2433
Size: 4238
SacI G_AGCT'C
Cuts at: 326 1252 326
Size: 926 3312
SacII CC_GC'GG
Cuts at: 118 118
Size: 4238
SalI G'TCGA_C
Cuts at: 1275 1275
Size: 4238
SapI GCTCTTCn'nnn_
Cuts at: 331 2261 2471 331
Size: 1930 210 2098
Fragments arranged by size:
2098 1930 210
SbfI CC_TGCA'GG
Cuts at: 1285 1285
Size: 4238
ScaI AGT'ACT
Cuts at: 3755 3755
Size: 4238
SfcI C'TryA_G
Cuts at: 33 507 706 773 926 1067 1281 1966 2959
Size: 474 199 67 153 141 214 685 993
Cuts at: 2959 3150 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 474 214 199 191 153
141 67
SfoI GGC'GCC
Cuts at: 1670 1918 1670
Size: 248 3990
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4238
SmaI CCC'GGG
Cuts at: 1260 1260
Size: 4238
SmlI C'TyrA_G
Cuts at: 197 1393 2308 2800 3062 197
Size: 1196 915 492 262 1373
Fragments arranged by size:
1373 1196 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4238
SphI G_CATG'C
Cuts at: 1230 1291 1791 1230
Size: 61 500 3677
Fragments arranged by size:
3677 500 61
StuI AGG'CCT
Cuts at: 209 661 209
Size: 452 3786
StyI C'CwwG_G
Cuts at: 293 1372 1726 293
Size: 1079 354 2805
Fragments arranged by size:
2805 1079 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 824 1821 1954 2413 2622 824
Size: 997 133 459 209 2440
Fragments arranged by size:
2440 997 459 209 133
TatI w'GTAC_w
Cuts at: 375 3753 375
Size: 3378 860
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3755 3787 3755
Size: 32 4206
Tth111I GACn'n_nGTC
Cuts at: 139 2035 139
Size: 1896 2342
XbaI T'CTAG_A
Cuts at: 1269 1269
Size: 4238
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 351 1733 351
Size: 1382 2856
XmaI C'CCGG_G
Cuts at: 1258 1258
Size: 4238
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NdeI NgoMIV NspI PasI PciI PmeI
PpiI PpuMI PsiI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BfrBI BmgBI BmtI BpmI
BsaXI BsgI BsiWI BsmI BsrGI BssHII BstEII BstZ17I
ClaI DraIII EcoRV FseI FspAI HpaI MfeI NcoI
NheI NotI NruI NsiI PacI PflMI PfoI PmlI
PshAI PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI