(Circular) (Six-base) MAPSORT of: pF1KE0262.seq Check: 2371 from: 1 to: 3566 pF1KE0262 3566 bp With 182 enzymes: SgfI * April 24, 2009 13:12 .. AccI GT'mk_AC Cuts at: 604 604 Size: 3566 Acc65I G'GTAC_C Cuts at: 582 582 Size: 3566 AclI AA'CG_TT Cuts at: 3406 3406 Size: 3566 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 304 1391 1823 2570 2838 304 Size: 1087 432 747 268 1032 Fragments arranged by size: 1087 1032 747 432 268 AflIII A'CryG_T Cuts at: 918 2022 2999 918 Size: 1104 977 1485 Fragments arranged by size: 1485 1104 977 AgeI A'CCGG_T Cuts at: 1954 1954 Size: 3566 AlwNI CAG_nnn'CTG Cuts at: 871 2438 871 Size: 1567 1999 ApaI G_GGCC'C Cuts at: 218 218 Size: 3566 ApaLI G'TGCA_C Cuts at: 2336 2336 Size: 3566 ApoI r'AATT_y Cuts at: 570 2919 3175 570 Size: 2349 256 961 Fragments arranged by size: 2349 961 256 AseI AT'TA_AT Cuts at: 20 20 Size: 3566 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3566 AvaI C'yCGr_G Cuts at: 586 586 Size: 3566 BamHI G'GATC_C Cuts at: 591 591 Size: 3566 BanI G'GyrC_C Cuts at: 582 996 1244 1279 582 Size: 414 248 35 2869 Fragments arranged by size: 2869 414 248 35 BanII G_rGCy'C Cuts at: 218 457 580 1610 218 Size: 239 123 1030 2174 Fragments arranged by size: 2174 1030 239 123 BbeI G_GCGC'C Cuts at: 1000 1248 1000 Size: 248 3318 BciVI GTATCCnnnnn_n' Cuts at: 1464 2225 3566 1464 Size: 761 1341 1464 Fragments arranged by size: 1464 1341 761 BclI T'GATC_A Cuts at: 1086 1086 Size: 3566 BglI GCCn_nnn'nGGC Cuts at: 274 274 Size: 3566 BglII A'GATC_T Cuts at: 1081 3118 1081 Size: 2037 1529 BlpI GC'TnA_GC Cuts at: 678 678 Size: 3566 Bme1580I G_kGCm'C Cuts at: 218 1191 1284 2340 218 Size: 973 93 1056 1444 Fragments arranged by size: 1444 1056 973 93 BmgBI CAC'GTC Cuts at: 153 153 Size: 3566 BmrI ACTGGGnnnn_n' Cuts at: 778 859 1194 3338 778 Size: 81 335 2144 1006 Fragments arranged by size: 2144 1006 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 252 1933 252 Size: 1681 1885 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 533 533 Size: 3566 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 742 1621 2113 2411 742 Size: 879 492 298 1897 Fragments arranged by size: 1897 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 3270 3270 Size: 3566 BsaAI yAC'GTr Cuts at: 919 919 Size: 3566 BsaBI GATnn'nnATC Cuts at: 6 1080 3117 6 Size: 1074 2037 455 Fragments arranged by size: 2037 1074 455 BsaHI Gr'CG_yC Cuts at: 997 1245 1947 3443 997 Size: 248 702 1496 1120 Fragments arranged by size: 1496 1120 702 248 BsaWI w'CCGG_w Cuts at: 140 755 1276 1954 2228 2375 2786 140 Size: 615 521 678 274 147 411 920 Fragments arranged by size: 920 678 615 521 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 530 530 Size: 3566 BseYI C'CCAG_C Cuts at: 991 1749 2326 2822 991 Size: 758 577 496 1735 Fragments arranged by size: 1735 758 577 496 BsiEI CG_ry'CG Cuts at: 104 1108 1154 2362 104 Size: 1004 46 1208 1308 Fragments arranged by size: 1308 1208 1004 46 BsiHKAI G_wGCw'C Cuts at: 356 580 1358 1548 2340 356 Size: 224 778 190 792 1582 Fragments arranged by size: 1582 792 778 224 190 Bsp1286I G_dGCh'C Cuts at: 218 356 457 580 1191 1284 1358 1548 1610 Size: 138 101 123 611 93 74 190 62 Cuts at: 1610 2340 218 Size: 730 1444 Fragments arranged by size: 1444 730 611 190 138 123 101 93 74 62 BspEI T'CCGG_A Cuts at: 140 2786 140 Size: 2646 920 BspHI T'CATG_A Cuts at: 2742 2742 Size: 3566 BspMI ACCTGCnnnn'nnnn_ Cuts at: 616 830 1132 1513 616 Size: 214 302 381 2669 Fragments arranged by size: 2669 381 302 214 BsrBI CCG'CTC Cuts at: 50 1858 1912 3396 3561 50 Size: 1808 54 1484 165 55 Fragments arranged by size: 1808 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 493 1478 493 Size: 985 2581 BsrFI r'CCGG_y Cuts at: 1564 1745 1954 1564 Size: 181 209 3176 Fragments arranged by size: 3176 209 181 BssSI C'ACGA_G Cuts at: 1837 2195 1837 Size: 358 3208 BstAPI GCAn_nnn'nTGC Cuts at: 508 943 508 Size: 435 3131 BstBI TT'CG_AA Cuts at: 1927 1927 Size: 3566 BstYI r'GATC_y Cuts at: 1 417 591 1058 1081 1416 1662 2674 2760 Size: 416 174 467 23 335 246 1012 86 Cuts at: 2760 2772 3118 1 Size: 12 346 449 Fragments arranged by size: 1012 467 449 416 346 335 246 174 86 23 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1516 1697 1860 1516 Size: 181 163 3222 Fragments arranged by size: 3222 181 163 BtsI GCAGTG_nn' Cuts at: 323 477 809 323 Size: 154 332 3080 Fragments arranged by size: 3080 332 154 DraI TTT'AAA Cuts at: 366 565 366 Size: 199 3367 DrdI GACnn_nn'nnGTC Cuts at: 1272 2130 1272 Size: 858 2708 EaeI y'GGCC_r Cuts at: 204 1151 1325 1716 1743 3088 204 Size: 947 174 391 27 1345 682 Fragments arranged by size: 1345 947 682 391 174 27 EagI C'GGCC_G Cuts at: 1151 1151 Size: 3566 EarI CTCTTCn'nnn_ Cuts at: 1589 1799 1589 Size: 210 3356 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1774 2084 2230 3376 1774 Size: 310 146 1146 1964 Fragments arranged by size: 1964 1146 310 146 EcoICRI GAG'CTC Cuts at: 578 578 Size: 3566 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 304 533 1391 1823 2570 2838 304 Size: 229 858 432 747 268 1032 Fragments arranged by size: 1032 858 747 432 268 229 EcoO109I rG'GnC_Cy Cuts at: 705 705 Size: 3566 EcoRI G'AATT_C Cuts at: 570 570 Size: 3566 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1585 1585 Size: 3566 FspI TGC'GCA Cuts at: 1347 1347 Size: 3566 HaeII r_GCGC'y Cuts at: 1000 1248 2270 3249 1000 Size: 248 1022 979 1317 Fragments arranged by size: 1317 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 442 474 1408 1438 1440 1470 442 Size: 32 934 30 2 30 2538 Fragments arranged by size: 2538 934 32 30 30 2 HincII GTy'rAC Cuts at: 605 605 Size: 3566 KasI G'GCGC_C Cuts at: 996 1244 996 Size: 248 3318 KpnI G_GTAC'C Cuts at: 586 586 Size: 3566 MluI A'CGCG_T Cuts at: 2999 2999 Size: 3566 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2237 2421 2237 Size: 184 3382 MscI TGG'CCA Cuts at: 1327 1327 Size: 3566 MslI CAynn'nnrTG Cuts at: 1555 1682 1555 Size: 127 3439 MspA1I CmG'CkG Cuts at: 265 677 991 1351 2364 2609 265 Size: 412 314 360 1013 245 1222 Fragments arranged by size: 1222 1013 412 360 314 245 NaeI GCC'GGC Cuts at: 1747 1747 Size: 3566 NarI GG'CG_CC Cuts at: 997 1245 997 Size: 248 3318 NgoMIV G'CCGG_C Cuts at: 1745 1745 Size: 3566 NspI r_CATG'y Cuts at: 619 1119 2026 619 Size: 500 907 2159 Fragments arranged by size: 2159 907 500 PciI A'CATG_T Cuts at: 2022 2022 Size: 3566 PmeI GTTT'AAAC Cuts at: 565 565 Size: 3566 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 160 192 2705 2737 160 Size: 32 2513 32 989 Fragments arranged by size: 2513 989 32 32 PsiI TTA'TAA Cuts at: 440 440 Size: 3566 PspOMI G'GGCC_C Cuts at: 214 214 Size: 3566 PstI C_TGCA'G Cuts at: 613 1298 613 Size: 685 2881 PvuI CG_AT'CG Cuts at: 104 104 Size: 3566 PvuII CAG'CTG Cuts at: 265 991 1351 265 Size: 726 360 2480 Fragments arranged by size: 2480 726 360 RsrII CG'GwC_CG Cuts at: 1761 1761 Size: 3566 SacI G_AGCT'C Cuts at: 580 580 Size: 3566 SalI G'TCGA_C Cuts at: 603 603 Size: 3566 SapI GCTCTTCn'nnn_ Cuts at: 1589 1799 1589 Size: 210 3356 SbfI CC_TGCA'GG Cuts at: 613 613 Size: 3566 ScaI AGT'ACT Cuts at: 3083 3083 Size: 3566 SfcI C'TryA_G Cuts at: 33 271 609 1294 2287 2478 33 Size: 238 338 685 993 191 1121 Fragments arranged by size: 1121 993 685 338 238 191 SfoI GGC'GCC Cuts at: 998 1246 998 Size: 248 3318 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3566 SmaI CCC'GGG Cuts at: 588 588 Size: 3566 SmlI C'TyrA_G Cuts at: 721 1636 2128 2390 721 Size: 915 492 262 1897 Fragments arranged by size: 1897 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 3566 SphI G_CATG'C Cuts at: 619 1119 619 Size: 500 3066 StyI C'CwwG_G Cuts at: 700 1054 700 Size: 354 3212 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1149 1282 1741 1950 1149 Size: 133 459 209 2765 Fragments arranged by size: 2765 459 209 133 TatI w'GTAC_w Cuts at: 3081 3081 Size: 3566 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3083 3115 3083 Size: 32 3534 Tth111I GACn'n_nGTC Cuts at: 1363 1363 Size: 3566 XbaI T'CTAG_A Cuts at: 597 597 Size: 3566 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1061 1061 Size: 3566 XmaI C'CCGG_G Cuts at: 586 586 Size: 3566 XmnI GAAnn'nnTTC Cuts at: 175 175 Size: 3566 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI PmeI PpiI PsiI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AlfI AloI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmtI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI