Restriction map for
pF1KE0262
(Circular) (Six-base) MAPSORT of: pF1KE0262.seq Check: 2371 from: 1 to: 3566
pF1KE0262 3566 bp
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AccI GT'mk_AC
Cuts at: 604 604
Size: 3566
Acc65I G'GTAC_C
Cuts at: 582 582
Size: 3566
AclI AA'CG_TT
Cuts at: 3406 3406
Size: 3566
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 304 1391 1823 2570 2838 304
Size: 1087 432 747 268 1032
Fragments arranged by size:
1087 1032 747 432 268
AflIII A'CryG_T
Cuts at: 918 2022 2999 918
Size: 1104 977 1485
Fragments arranged by size:
1485 1104 977
AgeI A'CCGG_T
Cuts at: 1954 1954
Size: 3566
AlwNI CAG_nnn'CTG
Cuts at: 871 2438 871
Size: 1567 1999
ApaI G_GGCC'C
Cuts at: 218 218
Size: 3566
ApaLI G'TGCA_C
Cuts at: 2336 2336
Size: 3566
ApoI r'AATT_y
Cuts at: 570 2919 3175 570
Size: 2349 256 961
Fragments arranged by size:
2349 961 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3566
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3566
AvaI C'yCGr_G
Cuts at: 586 586
Size: 3566
BamHI G'GATC_C
Cuts at: 591 591
Size: 3566
BanI G'GyrC_C
Cuts at: 582 996 1244 1279 582
Size: 414 248 35 2869
Fragments arranged by size:
2869 414 248 35
BanII G_rGCy'C
Cuts at: 218 457 580 1610 218
Size: 239 123 1030 2174
Fragments arranged by size:
2174 1030 239 123
BbeI G_GCGC'C
Cuts at: 1000 1248 1000
Size: 248 3318
BciVI GTATCCnnnnn_n'
Cuts at: 1464 2225 3566 1464
Size: 761 1341 1464
Fragments arranged by size:
1464 1341 761
BclI T'GATC_A
Cuts at: 1086 1086
Size: 3566
BglI GCCn_nnn'nGGC
Cuts at: 274 274
Size: 3566
BglII A'GATC_T
Cuts at: 1081 3118 1081
Size: 2037 1529
BlpI GC'TnA_GC
Cuts at: 678 678
Size: 3566
Bme1580I G_kGCm'C
Cuts at: 218 1191 1284 2340 218
Size: 973 93 1056 1444
Fragments arranged by size:
1444 1056 973 93
BmgBI CAC'GTC
Cuts at: 153 153
Size: 3566
BmrI ACTGGGnnnn_n'
Cuts at: 778 859 1194 3338 778
Size: 81 335 2144 1006
Fragments arranged by size:
2144 1006 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 252 1933 252
Size: 1681 1885
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 533 533
Size: 3566
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 742 1621 2113 2411 742
Size: 879 492 298 1897
Fragments arranged by size:
1897 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3270 3270
Size: 3566
BsaAI yAC'GTr
Cuts at: 919 919
Size: 3566
BsaBI GATnn'nnATC
Cuts at: 6 1080 3117 6
Size: 1074 2037 455
Fragments arranged by size:
2037 1074 455
BsaHI Gr'CG_yC
Cuts at: 997 1245 1947 3443 997
Size: 248 702 1496 1120
Fragments arranged by size:
1496 1120 702 248
BsaWI w'CCGG_w
Cuts at: 140 755 1276 1954 2228 2375 2786 140
Size: 615 521 678 274 147 411 920
Fragments arranged by size:
920 678 615 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 530 530
Size: 3566
BseYI C'CCAG_C
Cuts at: 991 1749 2326 2822 991
Size: 758 577 496 1735
Fragments arranged by size:
1735 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1108 1154 2362 104
Size: 1004 46 1208 1308
Fragments arranged by size:
1308 1208 1004 46
BsiHKAI G_wGCw'C
Cuts at: 356 580 1358 1548 2340 356
Size: 224 778 190 792 1582
Fragments arranged by size:
1582 792 778 224 190
Bsp1286I G_dGCh'C
Cuts at: 218 356 457 580 1191 1284 1358 1548 1610
Size: 138 101 123 611 93 74 190 62
Cuts at: 1610 2340 218
Size: 730 1444
Fragments arranged by size:
1444 730 611 190 138 123 101 93
74 62
BspEI T'CCGG_A
Cuts at: 140 2786 140
Size: 2646 920
BspHI T'CATG_A
Cuts at: 2742 2742
Size: 3566
BspMI ACCTGCnnnn'nnnn_
Cuts at: 616 830 1132 1513 616
Size: 214 302 381 2669
Fragments arranged by size:
2669 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1858 1912 3396 3561 50
Size: 1808 54 1484 165 55
Fragments arranged by size:
1808 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 493 1478 493
Size: 985 2581
BsrFI r'CCGG_y
Cuts at: 1564 1745 1954 1564
Size: 181 209 3176
Fragments arranged by size:
3176 209 181
BssSI C'ACGA_G
Cuts at: 1837 2195 1837
Size: 358 3208
BstAPI GCAn_nnn'nTGC
Cuts at: 508 943 508
Size: 435 3131
BstBI TT'CG_AA
Cuts at: 1927 1927
Size: 3566
BstYI r'GATC_y
Cuts at: 1 417 591 1058 1081 1416 1662 2674 2760
Size: 416 174 467 23 335 246 1012 86
Cuts at: 2760 2772 3118 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 416 346 335 246 174
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1516 1697 1860 1516
Size: 181 163 3222
Fragments arranged by size:
3222 181 163
BtsI GCAGTG_nn'
Cuts at: 323 477 809 323
Size: 154 332 3080
Fragments arranged by size:
3080 332 154
DraI TTT'AAA
Cuts at: 366 565 366
Size: 199 3367
DrdI GACnn_nn'nnGTC
Cuts at: 1272 2130 1272
Size: 858 2708
EaeI y'GGCC_r
Cuts at: 204 1151 1325 1716 1743 3088 204
Size: 947 174 391 27 1345 682
Fragments arranged by size:
1345 947 682 391 174 27
EagI C'GGCC_G
Cuts at: 1151 1151
Size: 3566
EarI CTCTTCn'nnn_
Cuts at: 1589 1799 1589
Size: 210 3356
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1774 2084 2230 3376 1774
Size: 310 146 1146 1964
Fragments arranged by size:
1964 1146 310 146
EcoICRI GAG'CTC
Cuts at: 578 578
Size: 3566
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 304 533 1391 1823 2570 2838 304
Size: 229 858 432 747 268 1032
Fragments arranged by size:
1032 858 747 432 268 229
EcoO109I rG'GnC_Cy
Cuts at: 705 705
Size: 3566
EcoRI G'AATT_C
Cuts at: 570 570
Size: 3566
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1585 1585
Size: 3566
FspI TGC'GCA
Cuts at: 1347 1347
Size: 3566
HaeII r_GCGC'y
Cuts at: 1000 1248 2270 3249 1000
Size: 248 1022 979 1317
Fragments arranged by size:
1317 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 442 474 1408 1438 1440 1470 442
Size: 32 934 30 2 30 2538
Fragments arranged by size:
2538 934 32 30 30 2
HincII GTy'rAC
Cuts at: 605 605
Size: 3566
KasI G'GCGC_C
Cuts at: 996 1244 996
Size: 248 3318
KpnI G_GTAC'C
Cuts at: 586 586
Size: 3566
MluI A'CGCG_T
Cuts at: 2999 2999
Size: 3566
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2237 2421 2237
Size: 184 3382
MscI TGG'CCA
Cuts at: 1327 1327
Size: 3566
MslI CAynn'nnrTG
Cuts at: 1555 1682 1555
Size: 127 3439
MspA1I CmG'CkG
Cuts at: 265 677 991 1351 2364 2609 265
Size: 412 314 360 1013 245 1222
Fragments arranged by size:
1222 1013 412 360 314 245
NaeI GCC'GGC
Cuts at: 1747 1747
Size: 3566
NarI GG'CG_CC
Cuts at: 997 1245 997
Size: 248 3318
NgoMIV G'CCGG_C
Cuts at: 1745 1745
Size: 3566
NspI r_CATG'y
Cuts at: 619 1119 2026 619
Size: 500 907 2159
Fragments arranged by size:
2159 907 500
PciI A'CATG_T
Cuts at: 2022 2022
Size: 3566
PmeI GTTT'AAAC
Cuts at: 565 565
Size: 3566
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 160 192 2705 2737 160
Size: 32 2513 32 989
Fragments arranged by size:
2513 989 32 32
PsiI TTA'TAA
Cuts at: 440 440
Size: 3566
PspOMI G'GGCC_C
Cuts at: 214 214
Size: 3566
PstI C_TGCA'G
Cuts at: 613 1298 613
Size: 685 2881
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3566
PvuII CAG'CTG
Cuts at: 265 991 1351 265
Size: 726 360 2480
Fragments arranged by size:
2480 726 360
RsrII CG'GwC_CG
Cuts at: 1761 1761
Size: 3566
SacI G_AGCT'C
Cuts at: 580 580
Size: 3566
SalI G'TCGA_C
Cuts at: 603 603
Size: 3566
SapI GCTCTTCn'nnn_
Cuts at: 1589 1799 1589
Size: 210 3356
SbfI CC_TGCA'GG
Cuts at: 613 613
Size: 3566
ScaI AGT'ACT
Cuts at: 3083 3083
Size: 3566
SfcI C'TryA_G
Cuts at: 33 271 609 1294 2287 2478 33
Size: 238 338 685 993 191 1121
Fragments arranged by size:
1121 993 685 338 238 191
SfoI GGC'GCC
Cuts at: 998 1246 998
Size: 248 3318
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3566
SmaI CCC'GGG
Cuts at: 588 588
Size: 3566
SmlI C'TyrA_G
Cuts at: 721 1636 2128 2390 721
Size: 915 492 262 1897
Fragments arranged by size:
1897 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3566
SphI G_CATG'C
Cuts at: 619 1119 619
Size: 500 3066
StyI C'CwwG_G
Cuts at: 700 1054 700
Size: 354 3212
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1149 1282 1741 1950 1149
Size: 133 459 209 2765
Fragments arranged by size:
2765 459 209 133
TatI w'GTAC_w
Cuts at: 3081 3081
Size: 3566
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3083 3115 3083
Size: 32 3534
Tth111I GACn'n_nGTC
Cuts at: 1363 1363
Size: 3566
XbaI T'CTAG_A
Cuts at: 597 597
Size: 3566
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1061 1061
Size: 3566
XmaI C'CCGG_G
Cuts at: 586 586
Size: 3566
XmnI GAAnn'nnTTC
Cuts at: 175 175
Size: 3566
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PmeI PpiI PsiI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmtI
Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI