Restriction map for
pF1KE0263
(Circular) (Six-base) MAPSORT of: pF1KE0263.seq Check: 784 from: 1 to: 3509
pF1KE0263 3509 bp
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AccI GT'mk_AC
Cuts at: 547 547
Size: 3509
Acc65I G'GTAC_C
Cuts at: 525 525
Size: 3509
AclI AA'CG_TT
Cuts at: 3349 3349
Size: 3509
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1334 1766 2513 2781 1334
Size: 432 747 268 2062
Fragments arranged by size:
2062 747 432 268
AflIII A'CryG_T
Cuts at: 861 1965 2942 861
Size: 1104 977 1428
Fragments arranged by size:
1428 1104 977
AgeI A'CCGG_T
Cuts at: 1897 1897
Size: 3509
AlwNI CAG_nnn'CTG
Cuts at: 814 2381 814
Size: 1567 1942
ApaLI G'TGCA_C
Cuts at: 2279 2279
Size: 3509
ApoI r'AATT_y
Cuts at: 186 316 513 2862 3118 186
Size: 130 197 2349 256 577
Fragments arranged by size:
2349 577 256 197 130
AseI AT'TA_AT
Cuts at: 20 20
Size: 3509
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3509
AvaI C'yCGr_G
Cuts at: 529 529
Size: 3509
BamHI G'GATC_C
Cuts at: 534 534
Size: 3509
BanI G'GyrC_C
Cuts at: 525 939 1187 1222 525
Size: 414 248 35 2812
Fragments arranged by size:
2812 414 248 35
BanII G_rGCy'C
Cuts at: 119 523 1553 119
Size: 404 1030 2075
Fragments arranged by size:
2075 1030 404
BbeI G_GCGC'C
Cuts at: 943 1191 943
Size: 248 3261
BciVI GTATCCnnnnn_n'
Cuts at: 1407 2168 3509 1407
Size: 761 1341 1407
Fragments arranged by size:
1407 1341 761
BclI T'GATC_A
Cuts at: 1029 1029
Size: 3509
BglII A'GATC_T
Cuts at: 293 1024 3061 293
Size: 731 2037 741
Fragments arranged by size:
2037 741 731
BlpI GC'TnA_GC
Cuts at: 621 621
Size: 3509
Bme1580I G_kGCm'C
Cuts at: 1134 1227 2283 1134
Size: 93 1056 2360
Fragments arranged by size:
2360 1056 93
BmgBI CAC'GTC
Cuts at: 390 390
Size: 3509
BmrI ACTGGGnnnn_n'
Cuts at: 187 721 802 1137 3281 187
Size: 534 81 335 2144 415
Fragments arranged by size:
2144 534 415 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 393 1876 393
Size: 1483 2026
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 129 129
Size: 3509
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 685 1564 2056 2354 685
Size: 879 492 298 1840
Fragments arranged by size:
1840 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 147 3213 147
Size: 3066 443
BsaAI yAC'GTr
Cuts at: 862 862
Size: 3509
BsaBI GATnn'nnATC
Cuts at: 6 1023 3060 6
Size: 1017 2037 455
Fragments arranged by size:
2037 1017 455
BsaHI Gr'CG_yC
Cuts at: 940 1188 1890 3386 940
Size: 248 702 1496 1063
Fragments arranged by size:
1496 1063 702 248
BsaWI w'CCGG_w
Cuts at: 341 698 1219 1897 2171 2318 2729 341
Size: 357 521 678 274 147 411 1121
Fragments arranged by size:
1121 678 521 411 357 274 147
BseYI C'CCAG_C
Cuts at: 306 934 1692 2269 2765 306
Size: 628 758 577 496 1050
Fragments arranged by size:
1050 758 628 577 496
BsiEI CG_ry'CG
Cuts at: 104 1051 1097 2305 104
Size: 947 46 1208 1308
Fragments arranged by size:
1308 1208 947 46
BsiHKAI G_wGCw'C
Cuts at: 358 523 1301 1491 2283 358
Size: 165 778 190 792 1584
Fragments arranged by size:
1584 792 778 190 165
Bsp1286I G_dGCh'C
Cuts at: 119 358 523 1134 1227 1301 1491 1553 2283
Size: 239 165 611 93 74 190 62 730
Cuts at: 2283 119
Size: 1345
Fragments arranged by size:
1345 730 611 239 190 165 93 74
62
BspEI T'CCGG_A
Cuts at: 2729 2729
Size: 3509
BspHI T'CATG_A
Cuts at: 2685 2685
Size: 3509
BspMI ACCTGCnnnn'nnnn_
Cuts at: 559 773 1075 1456 559
Size: 214 302 381 2612
Fragments arranged by size:
2612 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1801 1855 3339 3504 50
Size: 1751 54 1484 165 55
Fragments arranged by size:
1751 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1421 1421
Size: 3509
BsrFI r'CCGG_y
Cuts at: 1507 1688 1897 1507
Size: 181 209 3119
Fragments arranged by size:
3119 209 181
BssSI C'ACGA_G
Cuts at: 1780 2138 1780
Size: 358 3151
BstAPI GCAn_nnn'nTGC
Cuts at: 886 886
Size: 3509
BstBI TT'CG_AA
Cuts at: 1870 1870
Size: 3509
BstYI r'GATC_y
Cuts at: 1 293 534 1001 1024 1359 1605 2617 2703
Size: 292 241 467 23 335 246 1012 86
Cuts at: 2703 2715 3061 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 346 335 292 246 241
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3509
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1459 1640 1803 1459
Size: 181 163 3165
Fragments arranged by size:
3165 181 163
BtsI GCAGTG_nn'
Cuts at: 752 752
Size: 3509
DraI TTT'AAA
Cuts at: 508 508
Size: 3509
DrdI GACnn_nn'nnGTC
Cuts at: 1215 2073 1215
Size: 858 2651
EaeI y'GGCC_r
Cuts at: 1094 1268 1659 1686 3031 1094
Size: 174 391 27 1345 1572
Fragments arranged by size:
1572 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1094 1094
Size: 3509
EarI CTCTTCn'nnn_
Cuts at: 1532 1742 1532
Size: 210 3299
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1717 2027 2173 3319 1717
Size: 310 146 1146 1907
Fragments arranged by size:
1907 1146 310 146
EcoICRI GAG'CTC
Cuts at: 521 521
Size: 3509
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 129 1334 1766 2513 2781 129
Size: 1205 432 747 268 857
Fragments arranged by size:
1205 857 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 648 648
Size: 3509
EcoRI G'AATT_C
Cuts at: 513 513
Size: 3509
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 315 1528 315
Size: 1213 2296
FspI TGC'GCA
Cuts at: 1290 1290
Size: 3509
HaeII r_GCGC'y
Cuts at: 943 1191 2213 3192 943
Size: 248 1022 979 1260
Fragments arranged by size:
1260 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1351 1381 1383 1413 1351
Size: 30 2 30 3447
Fragments arranged by size:
3447 30 30 2
HincII GTy'rAC
Cuts at: 548 548
Size: 3509
KasI G'GCGC_C
Cuts at: 939 1187 939
Size: 248 3261
KpnI G_GTAC'C
Cuts at: 529 529
Size: 3509
MluI A'CGCG_T
Cuts at: 2942 2942
Size: 3509
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2180 2364 2180
Size: 184 3325
MscI TGG'CCA
Cuts at: 1270 1270
Size: 3509
MslI CAynn'nnrTG
Cuts at: 1498 1625 1498
Size: 127 3382
MspA1I CmG'CkG
Cuts at: 620 934 1294 2307 2552 620
Size: 314 360 1013 245 1577
Fragments arranged by size:
1577 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1690 1690
Size: 3509
NarI GG'CG_CC
Cuts at: 940 1188 940
Size: 248 3261
NcoI C'CATG_G
Cuts at: 107 107
Size: 3509
NgoMIV G'CCGG_C
Cuts at: 1688 1688
Size: 3509
NspI r_CATG'y
Cuts at: 562 1062 1969 562
Size: 500 907 2102
Fragments arranged by size:
2102 907 500
PciI A'CATG_T
Cuts at: 1965 1965
Size: 3509
PfoI T'CCnGG_A
Cuts at: 281 281
Size: 3509
PmeI GTTT'AAAC
Cuts at: 508 508
Size: 3509
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2648 2680 2648
Size: 32 3477
PstI C_TGCA'G
Cuts at: 556 1241 556
Size: 685 2824
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3509
PvuII CAG'CTG
Cuts at: 934 1294 934
Size: 360 3149
RsrII CG'GwC_CG
Cuts at: 1704 1704
Size: 3509
SacI G_AGCT'C
Cuts at: 523 523
Size: 3509
SalI G'TCGA_C
Cuts at: 546 546
Size: 3509
SapI GCTCTTCn'nnn_
Cuts at: 1532 1742 1532
Size: 210 3299
SbfI CC_TGCA'GG
Cuts at: 556 556
Size: 3509
ScaI AGT'ACT
Cuts at: 3026 3026
Size: 3509
SfcI C'TryA_G
Cuts at: 33 329 552 1237 2230 2421 33
Size: 296 223 685 993 191 1121
Fragments arranged by size:
1121 993 685 296 223 191
SfoI GGC'GCC
Cuts at: 941 1189 941
Size: 248 3261
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3509
SmaI CCC'GGG
Cuts at: 531 531
Size: 3509
SmlI C'TyrA_G
Cuts at: 664 1579 2071 2333 664
Size: 915 492 262 1840
Fragments arranged by size:
1840 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3509
SphI G_CATG'C
Cuts at: 562 1062 562
Size: 500 3009
SspI AAT'ATT
Cuts at: 474 474
Size: 3509
StyI C'CwwG_G
Cuts at: 107 643 997 107
Size: 536 354 2619
Fragments arranged by size:
2619 536 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1092 1225 1684 1893 1092
Size: 133 459 209 2708
Fragments arranged by size:
2708 459 209 133
TatI w'GTAC_w
Cuts at: 3024 3024
Size: 3509
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3026 3058 3026
Size: 32 3477
Tth111I GACn'n_nGTC
Cuts at: 1306 1306
Size: 3509
XbaI T'CTAG_A
Cuts at: 540 540
Size: 3509
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 400 1004 400
Size: 604 2905
XmaI C'CCGG_G
Cuts at: 529 529
Size: 3509
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PfoI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI
SwaI XhoI XmnI ZraI