Restriction map for
pF1KE0267
(Circular) (Six-base) MAPSORT of: pF1KE0267.seq Check: 8002 from: 1 to: 4058
pF1KE0267 4058 bp
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 981 981
Size: 4058
AatII G_ACGT'C
Cuts at: 547 547
Size: 4058
AccI GT'mk_AC
Cuts at: 1096 1096
Size: 4058
Acc65I G'GTAC_C
Cuts at: 1074 1074
Size: 4058
AclI AA'CG_TT
Cuts at: 3898 3898
Size: 4058
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1883 2315 3062 3330 1883
Size: 432 747 268 2611
Fragments arranged by size:
2611 747 432 268
AflIII A'CryG_T
Cuts at: 1410 2514 3491 1410
Size: 1104 977 1977
Fragments arranged by size:
1977 1104 977
AgeI A'CCGG_T
Cuts at: 2446 2446
Size: 4058
AlwNI CAG_nnn'CTG
Cuts at: 1363 2930 1363
Size: 1567 2491
ApaI G_GGCC'C
Cuts at: 141 141
Size: 4058
ApaLI G'TGCA_C
Cuts at: 2828 2828
Size: 4058
ApoI r'AATT_y
Cuts at: 1062 3411 3667 1062
Size: 2349 256 1453
Fragments arranged by size:
2349 1453 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4058
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4058
AvaI C'yCGr_G
Cuts at: 388 1078 388
Size: 690 3368
BamHI G'GATC_C
Cuts at: 1083 1083
Size: 4058
BanI G'GyrC_C
Cuts at: 337 384 460 816 959 993 1007 1074 1488
Size: 47 76 356 143 34 14 67 414
Cuts at: 1488 1736 1771 337
Size: 248 35 2624
Fragments arranged by size:
2624 414 356 248 143 76 67 47
35 34 14
BanII G_rGCy'C
Cuts at: 141 268 483 583 781 1072 2102 141
Size: 127 215 100 198 291 1030 2097
Fragments arranged by size:
2097 1030 291 215 198 127 100
BbeI G_GCGC'C
Cuts at: 341 388 464 1011 1492 1740 341
Size: 47 76 547 481 248 2659
Fragments arranged by size:
2659 547 481 248 76 47
BbsI GAAGACnn'nnnn_
Cuts at: 342 342
Size: 4058
BciVI GTATCCnnnnn_n'
Cuts at: 1956 2717 4058 1956
Size: 761 1341 1956
Fragments arranged by size:
1956 1341 761
BclI T'GATC_A
Cuts at: 1578 1578
Size: 4058
BglI GCCn_nnn'nGGC
Cuts at: 766 766
Size: 4058
BglII A'GATC_T
Cuts at: 1573 3610 1573
Size: 2037 2021
BlpI GC'TnA_GC
Cuts at: 1170 1170
Size: 4058
Bme1580I G_kGCm'C
Cuts at: 141 620 821 962 998 1683 1776 2832 141
Size: 479 201 141 36 685 93 1056 1367
Fragments arranged by size:
1367 1056 685 479 201 141 93 36
BmgBI CAC'GTC
Cuts at: 623 699 708 623
Size: 76 9 3973
Fragments arranged by size:
3973 76 9
BmrI ACTGGGnnnn_n'
Cuts at: 1270 1351 1686 3830 1270
Size: 81 335 2144 1498
Fragments arranged by size:
2144 1498 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2425 2425
Size: 4058
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 808 884 172
Size: 636 76 3346
Fragments arranged by size:
3346 636 76
Bpu10I CC'TnA_GC
Cuts at: 803 803
Size: 4058
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1234 2113 2605 2903 1234
Size: 879 492 298 2389
Fragments arranged by size:
2389 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3762 3762
Size: 4058
BsaAI yAC'GTr
Cuts at: 534 1411 534
Size: 877 3181
BsaBI GATnn'nnATC
Cuts at: 6 1572 3609 6
Size: 1566 2037 455
Fragments arranged by size:
2037 1566 455
BsaHI Gr'CG_yC
Cuts at: 338 385 461 544 1008 1489 1737 2439 3935
Size: 47 76 83 464 481 248 702 1496
Cuts at: 3935 338
Size: 461
Fragments arranged by size:
1496 702 481 464 461 248 83 76
47
BsaWI w'CCGG_w
Cuts at: 1247 1768 2446 2720 2867 3278 1247
Size: 521 678 274 147 411 2027
Fragments arranged by size:
2027 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 185 215 358 388 185
Size: 30 143 30 3855
Fragments arranged by size:
3855 143 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 112 250 112
Size: 138 3920
BseYI C'CCAG_C
Cuts at: 132 177 333 420 559 1483 2241 2818 3314
Size: 45 156 87 139 924 758 577 496
Cuts at: 3314 132
Size: 876
Fragments arranged by size:
924 876 758 577 496 156 139 87
45
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 421 721 1009 421
Size: 300 288 3470
Fragments arranged by size:
3470 300 288
BsiEI CG_ry'CG
Cuts at: 104 470 612 793 1600 1646 2854 104
Size: 366 142 181 807 46 1208 1308
Fragments arranged by size:
1308 1208 807 366 181 142 46
BsiHKAI G_wGCw'C
Cuts at: 281 781 809 1072 1850 2040 2832 281
Size: 500 28 263 778 190 792 1507
Fragments arranged by size:
1507 792 778 500 263 190 28
Bsp1286I G_dGCh'C
Cuts at: 141 268 281 483 583 620 781 809 821
Size: 127 13 202 100 37 161 28 12
Cuts at: 821 962 998 1072 1683 1776 1850 2040 2102
Size: 141 36 74 611 93 74 190 62
Cuts at: 2102 2832 141
Size: 730 1367
Fragments arranged by size:
1367 730 611 202 190 161 141 127
100 93 74 74 62 37 36 28
13 12
BspEI T'CCGG_A
Cuts at: 3278 3278
Size: 4058
BspHI T'CATG_A
Cuts at: 3234 3234
Size: 4058
BspMI ACCTGCnnnn'nnnn_
Cuts at: 504 981 1108 1322 1624 2005 504
Size: 477 127 214 302 381 2557
Fragments arranged by size:
2557 477 381 302 214 127
BsrBI CCG'CTC
Cuts at: 50 2350 2404 3888 4053 50
Size: 2300 54 1484 165 55
Fragments arranged by size:
2300 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 845 1970 845
Size: 1125 2933
BsrFI r'CCGG_y
Cuts at: 469 2056 2237 2446 469
Size: 1587 181 209 2081
Fragments arranged by size:
2081 1587 209 181
BssSI C'ACGA_G
Cuts at: 439 2329 2687 439
Size: 1890 358 1810
Fragments arranged by size:
1890 1810 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1435 1435
Size: 4058
BstBI TT'CG_AA
Cuts at: 2419 2419
Size: 4058
BstYI r'GATC_y
Cuts at: 1 1083 1550 1573 1908 2154 3166 3252 3264
Size: 1082 467 23 335 246 1012 86 12
Cuts at: 3264 3610 1
Size: 346 449
Fragments arranged by size:
1082 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 380 380
Size: 4058
BtgI C'CryG_G
Cuts at: 793 793
Size: 4058
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 342 2008 2189 2352 342
Size: 1666 181 163 2048
Fragments arranged by size:
2048 1666 181 163
BtsI GCAGTG_nn'
Cuts at: 281 1301 281
Size: 1020 3038
DraI TTT'AAA
Cuts at: 1057 1057
Size: 4058
DraIII CAC_nnn'GTG
Cuts at: 276 728 276
Size: 452 3606
DrdI GACnn_nn'nnGTC
Cuts at: 1764 2622 1764
Size: 858 3200
EaeI y'GGCC_r
Cuts at: 467 609 647 716 790 901 1044 1643 1817
Size: 142 38 69 74 111 143 599 174
Cuts at: 1817 2208 2235 3580 467
Size: 391 27 1345 945
Fragments arranged by size:
1345 945 599 391 174 143 142 111
74 69 38 27
EagI C'GGCC_G
Cuts at: 467 609 790 1643 467
Size: 142 181 853 2882
Fragments arranged by size:
2882 853 181 142
EarI CTCTTCn'nnn_
Cuts at: 2081 2291 2081
Size: 210 3848
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 379 747 2266 2576 2722 3868 379
Size: 368 1519 310 146 1146 569
Fragments arranged by size:
1519 1146 569 368 310 146
EcoICRI GAG'CTC
Cuts at: 779 1070 779
Size: 291 3767
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 808 884 1883 2315 3062 3330 172
Size: 636 76 999 432 747 268 900
Fragments arranged by size:
999 900 747 636 432 268 76
EcoNI CCTnn'n_nnAGG
Cuts at: 307 307
Size: 4058
EcoO109I rG'GnC_Cy
Cuts at: 137 138 1197 137
Size: 1 1059 2998
Fragments arranged by size:
2998 1059 1
EcoRI G'AATT_C
Cuts at: 1062 1062
Size: 4058
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2077 2077
Size: 4058
FspI TGC'GCA
Cuts at: 1839 1839
Size: 4058
HaeII r_GCGC'y
Cuts at: 341 388 464 556 1011 1492 1740 2762 3741
Size: 47 76 92 455 481 248 1022 979
Cuts at: 3741 341
Size: 658
Fragments arranged by size:
1022 979 658 481 455 248 92 76
47
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 358 390 739 771 1900 1930 1932 1962 358
Size: 32 349 32 1129 30 2 30 2454
Fragments arranged by size:
2454 1129 349 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1097 1097
Size: 4058
KasI G'GCGC_C
Cuts at: 337 384 460 1007 1488 1736 337
Size: 47 76 547 481 248 2659
Fragments arranged by size:
2659 547 481 248 76 47
KpnI G_GTAC'C
Cuts at: 1078 1078
Size: 4058
MluI A'CGCG_T
Cuts at: 3491 3491
Size: 4058
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 877 2729 2913 877
Size: 1852 184 2022
Fragments arranged by size:
2022 1852 184
MscI TGG'CCA
Cuts at: 718 903 1046 1819 718
Size: 185 143 773 2957
Fragments arranged by size:
2957 773 185 143
MslI CAynn'nnrTG
Cuts at: 112 724 2047 2174 112
Size: 612 1323 127 1996
Fragments arranged by size:
1996 1323 612 127
MspA1I CmG'CkG
Cuts at: 795 1169 1483 1843 2856 3101 795
Size: 374 314 360 1013 245 1752
Fragments arranged by size:
1752 1013 374 360 314 245
NaeI GCC'GGC
Cuts at: 471 2239 471
Size: 1768 2290
NarI GG'CG_CC
Cuts at: 338 385 461 1008 1489 1737 338
Size: 47 76 547 481 248 2659
Fragments arranged by size:
2659 547 481 248 76 47
NgoMIV G'CCGG_C
Cuts at: 469 2237 469
Size: 1768 2290
NspI r_CATG'y
Cuts at: 1111 1611 2518 1111
Size: 500 907 2651
Fragments arranged by size:
2651 907 500
PciI A'CATG_T
Cuts at: 2514 2514
Size: 4058
PmeI GTTT'AAAC
Cuts at: 1057 1057
Size: 4058
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3197 3229 3197
Size: 32 4026
PspOMI G'GGCC_C
Cuts at: 137 137
Size: 4058
PstI C_TGCA'G
Cuts at: 308 1105 1790 308
Size: 797 685 2576
Fragments arranged by size:
2576 797 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4058
PvuII CAG'CTG
Cuts at: 1483 1843 1483
Size: 360 3698
RsrII CG'GwC_CG
Cuts at: 2253 2253
Size: 4058
SacI G_AGCT'C
Cuts at: 781 1072 781
Size: 291 3767
SacII CC_GC'GG
Cuts at: 796 796
Size: 4058
SalI G'TCGA_C
Cuts at: 1095 1095
Size: 4058
SapI GCTCTTCn'nnn_
Cuts at: 2081 2291 2081
Size: 210 3848
SbfI CC_TGCA'GG
Cuts at: 1105 1105
Size: 4058
ScaI AGT'ACT
Cuts at: 3575 3575
Size: 4058
SexAI A'CCwGG_T
Cuts at: 272 272
Size: 4058
SfcI C'TryA_G
Cuts at: 33 304 1101 1786 2779 2970 33
Size: 271 797 685 993 191 1121
Fragments arranged by size:
1121 993 797 685 271 191
SfoI GGC'GCC
Cuts at: 339 386 462 1009 1490 1738 339
Size: 47 76 547 481 248 2659
Fragments arranged by size:
2659 547 481 248 76 47
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4058
SmaI CCC'GGG
Cuts at: 1080 1080
Size: 4058
SmlI C'TyrA_G
Cuts at: 1213 2128 2620 2882 1213
Size: 915 492 262 2389
Fragments arranged by size:
2389 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4058
SphI G_CATG'C
Cuts at: 1111 1611 1111
Size: 500 3558
StuI AGG'CCT
Cuts at: 1032 1032
Size: 4058
StyI C'CwwG_G
Cuts at: 344 1192 1546 344
Size: 848 354 2856
Fragments arranged by size:
2856 848 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1641 1774 2233 2442 1641
Size: 133 459 209 3257
Fragments arranged by size:
3257 459 209 133
TatI w'GTAC_w
Cuts at: 3573 3573
Size: 4058
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 185 217 3575 3607 185
Size: 32 3358 32 636
Fragments arranged by size:
3358 636 32 32
Tth111I GACn'n_nGTC
Cuts at: 1855 1855
Size: 4058
XbaI T'CTAG_A
Cuts at: 1089 1089
Size: 4058
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 657 732 977 1553 657
Size: 75 245 576 3162
Fragments arranged by size:
3162 576 245 75
XmaI C'CCGG_G
Cuts at: 1078 1078
Size: 4058
ZraI GAC'GTC
Cuts at: 545 545
Size: 4058
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PmeI
PpiI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AlfI AloI AscI AvrII
BaeI BbvCI BcgI BfrBI BmtI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PspXI PsrI SanDI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI