(Circular) (Six-base) MAPSORT of: pF1KE0267.seq Check: 8002 from: 1 to: 4058 pF1KE0267 4058 bp With 182 enzymes: SgfI * April 24, 2009 13:12 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 981 981 Size: 4058 AatII G_ACGT'C Cuts at: 547 547 Size: 4058 AccI GT'mk_AC Cuts at: 1096 1096 Size: 4058 Acc65I G'GTAC_C Cuts at: 1074 1074 Size: 4058 AclI AA'CG_TT Cuts at: 3898 3898 Size: 4058 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1883 2315 3062 3330 1883 Size: 432 747 268 2611 Fragments arranged by size: 2611 747 432 268 AflIII A'CryG_T Cuts at: 1410 2514 3491 1410 Size: 1104 977 1977 Fragments arranged by size: 1977 1104 977 AgeI A'CCGG_T Cuts at: 2446 2446 Size: 4058 AlwNI CAG_nnn'CTG Cuts at: 1363 2930 1363 Size: 1567 2491 ApaI G_GGCC'C Cuts at: 141 141 Size: 4058 ApaLI G'TGCA_C Cuts at: 2828 2828 Size: 4058 ApoI r'AATT_y Cuts at: 1062 3411 3667 1062 Size: 2349 256 1453 Fragments arranged by size: 2349 1453 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4058 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4058 AvaI C'yCGr_G Cuts at: 388 1078 388 Size: 690 3368 BamHI G'GATC_C Cuts at: 1083 1083 Size: 4058 BanI G'GyrC_C Cuts at: 337 384 460 816 959 993 1007 1074 1488 Size: 47 76 356 143 34 14 67 414 Cuts at: 1488 1736 1771 337 Size: 248 35 2624 Fragments arranged by size: 2624 414 356 248 143 76 67 47 35 34 14 BanII G_rGCy'C Cuts at: 141 268 483 583 781 1072 2102 141 Size: 127 215 100 198 291 1030 2097 Fragments arranged by size: 2097 1030 291 215 198 127 100 BbeI G_GCGC'C Cuts at: 341 388 464 1011 1492 1740 341 Size: 47 76 547 481 248 2659 Fragments arranged by size: 2659 547 481 248 76 47 BbsI GAAGACnn'nnnn_ Cuts at: 342 342 Size: 4058 BciVI GTATCCnnnnn_n' Cuts at: 1956 2717 4058 1956 Size: 761 1341 1956 Fragments arranged by size: 1956 1341 761 BclI T'GATC_A Cuts at: 1578 1578 Size: 4058 BglI GCCn_nnn'nGGC Cuts at: 766 766 Size: 4058 BglII A'GATC_T Cuts at: 1573 3610 1573 Size: 2037 2021 BlpI GC'TnA_GC Cuts at: 1170 1170 Size: 4058 Bme1580I G_kGCm'C Cuts at: 141 620 821 962 998 1683 1776 2832 141 Size: 479 201 141 36 685 93 1056 1367 Fragments arranged by size: 1367 1056 685 479 201 141 93 36 BmgBI CAC'GTC Cuts at: 623 699 708 623 Size: 76 9 3973 Fragments arranged by size: 3973 76 9 BmrI ACTGGGnnnn_n' Cuts at: 1270 1351 1686 3830 1270 Size: 81 335 2144 1498 Fragments arranged by size: 2144 1498 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2425 2425 Size: 4058 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 172 808 884 172 Size: 636 76 3346 Fragments arranged by size: 3346 636 76 Bpu10I CC'TnA_GC Cuts at: 803 803 Size: 4058 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1234 2113 2605 2903 1234 Size: 879 492 298 2389 Fragments arranged by size: 2389 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 3762 3762 Size: 4058 BsaAI yAC'GTr Cuts at: 534 1411 534 Size: 877 3181 BsaBI GATnn'nnATC Cuts at: 6 1572 3609 6 Size: 1566 2037 455 Fragments arranged by size: 2037 1566 455 BsaHI Gr'CG_yC Cuts at: 338 385 461 544 1008 1489 1737 2439 3935 Size: 47 76 83 464 481 248 702 1496 Cuts at: 3935 338 Size: 461 Fragments arranged by size: 1496 702 481 464 461 248 83 76 47 BsaWI w'CCGG_w Cuts at: 1247 1768 2446 2720 2867 3278 1247 Size: 521 678 274 147 411 2027 Fragments arranged by size: 2027 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 185 215 358 388 185 Size: 30 143 30 3855 Fragments arranged by size: 3855 143 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 112 250 112 Size: 138 3920 BseYI C'CCAG_C Cuts at: 132 177 333 420 559 1483 2241 2818 3314 Size: 45 156 87 139 924 758 577 496 Cuts at: 3314 132 Size: 876 Fragments arranged by size: 924 876 758 577 496 156 139 87 45 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 421 721 1009 421 Size: 300 288 3470 Fragments arranged by size: 3470 300 288 BsiEI CG_ry'CG Cuts at: 104 470 612 793 1600 1646 2854 104 Size: 366 142 181 807 46 1208 1308 Fragments arranged by size: 1308 1208 807 366 181 142 46 BsiHKAI G_wGCw'C Cuts at: 281 781 809 1072 1850 2040 2832 281 Size: 500 28 263 778 190 792 1507 Fragments arranged by size: 1507 792 778 500 263 190 28 Bsp1286I G_dGCh'C Cuts at: 141 268 281 483 583 620 781 809 821 Size: 127 13 202 100 37 161 28 12 Cuts at: 821 962 998 1072 1683 1776 1850 2040 2102 Size: 141 36 74 611 93 74 190 62 Cuts at: 2102 2832 141 Size: 730 1367 Fragments arranged by size: 1367 730 611 202 190 161 141 127 100 93 74 74 62 37 36 28 13 12 BspEI T'CCGG_A Cuts at: 3278 3278 Size: 4058 BspHI T'CATG_A Cuts at: 3234 3234 Size: 4058 BspMI ACCTGCnnnn'nnnn_ Cuts at: 504 981 1108 1322 1624 2005 504 Size: 477 127 214 302 381 2557 Fragments arranged by size: 2557 477 381 302 214 127 BsrBI CCG'CTC Cuts at: 50 2350 2404 3888 4053 50 Size: 2300 54 1484 165 55 Fragments arranged by size: 2300 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 845 1970 845 Size: 1125 2933 BsrFI r'CCGG_y Cuts at: 469 2056 2237 2446 469 Size: 1587 181 209 2081 Fragments arranged by size: 2081 1587 209 181 BssSI C'ACGA_G Cuts at: 439 2329 2687 439 Size: 1890 358 1810 Fragments arranged by size: 1890 1810 358 BstAPI GCAn_nnn'nTGC Cuts at: 1435 1435 Size: 4058 BstBI TT'CG_AA Cuts at: 2419 2419 Size: 4058 BstYI r'GATC_y Cuts at: 1 1083 1550 1573 1908 2154 3166 3252 3264 Size: 1082 467 23 335 246 1012 86 12 Cuts at: 3264 3610 1 Size: 346 449 Fragments arranged by size: 1082 1012 467 449 346 335 246 86 23 12 Bsu36I CC'TnA_GG Cuts at: 380 380 Size: 4058 BtgI C'CryG_G Cuts at: 793 793 Size: 4058 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 342 2008 2189 2352 342 Size: 1666 181 163 2048 Fragments arranged by size: 2048 1666 181 163 BtsI GCAGTG_nn' Cuts at: 281 1301 281 Size: 1020 3038 DraI TTT'AAA Cuts at: 1057 1057 Size: 4058 DraIII CAC_nnn'GTG Cuts at: 276 728 276 Size: 452 3606 DrdI GACnn_nn'nnGTC Cuts at: 1764 2622 1764 Size: 858 3200 EaeI y'GGCC_r Cuts at: 467 609 647 716 790 901 1044 1643 1817 Size: 142 38 69 74 111 143 599 174 Cuts at: 1817 2208 2235 3580 467 Size: 391 27 1345 945 Fragments arranged by size: 1345 945 599 391 174 143 142 111 74 69 38 27 EagI C'GGCC_G Cuts at: 467 609 790 1643 467 Size: 142 181 853 2882 Fragments arranged by size: 2882 853 181 142 EarI CTCTTCn'nnn_ Cuts at: 2081 2291 2081 Size: 210 3848 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 379 747 2266 2576 2722 3868 379 Size: 368 1519 310 146 1146 569 Fragments arranged by size: 1519 1146 569 368 310 146 EcoICRI GAG'CTC Cuts at: 779 1070 779 Size: 291 3767 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 172 808 884 1883 2315 3062 3330 172 Size: 636 76 999 432 747 268 900 Fragments arranged by size: 999 900 747 636 432 268 76 EcoNI CCTnn'n_nnAGG Cuts at: 307 307 Size: 4058 EcoO109I rG'GnC_Cy Cuts at: 137 138 1197 137 Size: 1 1059 2998 Fragments arranged by size: 2998 1059 1 EcoRI G'AATT_C Cuts at: 1062 1062 Size: 4058 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2077 2077 Size: 4058 FspI TGC'GCA Cuts at: 1839 1839 Size: 4058 HaeII r_GCGC'y Cuts at: 341 388 464 556 1011 1492 1740 2762 3741 Size: 47 76 92 455 481 248 1022 979 Cuts at: 3741 341 Size: 658 Fragments arranged by size: 1022 979 658 481 455 248 92 76 47 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 358 390 739 771 1900 1930 1932 1962 358 Size: 32 349 32 1129 30 2 30 2454 Fragments arranged by size: 2454 1129 349 32 32 30 30 2 HincII GTy'rAC Cuts at: 1097 1097 Size: 4058 KasI G'GCGC_C Cuts at: 337 384 460 1007 1488 1736 337 Size: 47 76 547 481 248 2659 Fragments arranged by size: 2659 547 481 248 76 47 KpnI G_GTAC'C Cuts at: 1078 1078 Size: 4058 MluI A'CGCG_T Cuts at: 3491 3491 Size: 4058 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 877 2729 2913 877 Size: 1852 184 2022 Fragments arranged by size: 2022 1852 184 MscI TGG'CCA Cuts at: 718 903 1046 1819 718 Size: 185 143 773 2957 Fragments arranged by size: 2957 773 185 143 MslI CAynn'nnrTG Cuts at: 112 724 2047 2174 112 Size: 612 1323 127 1996 Fragments arranged by size: 1996 1323 612 127 MspA1I CmG'CkG Cuts at: 795 1169 1483 1843 2856 3101 795 Size: 374 314 360 1013 245 1752 Fragments arranged by size: 1752 1013 374 360 314 245 NaeI GCC'GGC Cuts at: 471 2239 471 Size: 1768 2290 NarI GG'CG_CC Cuts at: 338 385 461 1008 1489 1737 338 Size: 47 76 547 481 248 2659 Fragments arranged by size: 2659 547 481 248 76 47 NgoMIV G'CCGG_C Cuts at: 469 2237 469 Size: 1768 2290 NspI r_CATG'y Cuts at: 1111 1611 2518 1111 Size: 500 907 2651 Fragments arranged by size: 2651 907 500 PciI A'CATG_T Cuts at: 2514 2514 Size: 4058 PmeI GTTT'AAAC Cuts at: 1057 1057 Size: 4058 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3197 3229 3197 Size: 32 4026 PspOMI G'GGCC_C Cuts at: 137 137 Size: 4058 PstI C_TGCA'G Cuts at: 308 1105 1790 308 Size: 797 685 2576 Fragments arranged by size: 2576 797 685 PvuI CG_AT'CG Cuts at: 104 104 Size: 4058 PvuII CAG'CTG Cuts at: 1483 1843 1483 Size: 360 3698 RsrII CG'GwC_CG Cuts at: 2253 2253 Size: 4058 SacI G_AGCT'C Cuts at: 781 1072 781 Size: 291 3767 SacII CC_GC'GG Cuts at: 796 796 Size: 4058 SalI G'TCGA_C Cuts at: 1095 1095 Size: 4058 SapI GCTCTTCn'nnn_ Cuts at: 2081 2291 2081 Size: 210 3848 SbfI CC_TGCA'GG Cuts at: 1105 1105 Size: 4058 ScaI AGT'ACT Cuts at: 3575 3575 Size: 4058 SexAI A'CCwGG_T Cuts at: 272 272 Size: 4058 SfcI C'TryA_G Cuts at: 33 304 1101 1786 2779 2970 33 Size: 271 797 685 993 191 1121 Fragments arranged by size: 1121 993 797 685 271 191 SfoI GGC'GCC Cuts at: 339 386 462 1009 1490 1738 339 Size: 47 76 547 481 248 2659 Fragments arranged by size: 2659 547 481 248 76 47 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4058 SmaI CCC'GGG Cuts at: 1080 1080 Size: 4058 SmlI C'TyrA_G Cuts at: 1213 2128 2620 2882 1213 Size: 915 492 262 2389 Fragments arranged by size: 2389 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4058 SphI G_CATG'C Cuts at: 1111 1611 1111 Size: 500 3558 StuI AGG'CCT Cuts at: 1032 1032 Size: 4058 StyI C'CwwG_G Cuts at: 344 1192 1546 344 Size: 848 354 2856 Fragments arranged by size: 2856 848 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1641 1774 2233 2442 1641 Size: 133 459 209 3257 Fragments arranged by size: 3257 459 209 133 TatI w'GTAC_w Cuts at: 3573 3573 Size: 4058 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 185 217 3575 3607 185 Size: 32 3358 32 636 Fragments arranged by size: 3358 636 32 32 Tth111I GACn'n_nGTC Cuts at: 1855 1855 Size: 4058 XbaI T'CTAG_A Cuts at: 1089 1089 Size: 4058 XcmI CCAnnnn_n'nnnnTGG Cuts at: 657 732 977 1553 657 Size: 75 245 576 3162 Fragments arranged by size: 3162 576 245 75 XmaI C'CCGG_G Cuts at: 1078 1078 Size: 4058 ZraI GAC'GTC Cuts at: 545 545 Size: 4058 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI PmeI PpiI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI Enzymes that do not cut: AfeI AflII AhdI AleI AlfI AloI AscI AvrII BaeI BbvCI BcgI BfrBI BmtI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspXI PsrI SanDI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI