Restriction map for
pF1KE0268
(Circular) (Six-base) MAPSORT of: pF1KE0268.seq Check: 5139 from: 1 to: 3773
pF1KE0268 3773 bp
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AccI GT'mk_AC
Cuts at: 734 811 734
Size: 77 3696
Acc65I G'GTAC_C
Cuts at: 789 789
Size: 3773
AclI AA'CG_TT
Cuts at: 3613 3613
Size: 3773
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1598 2030 2777 3045 1598
Size: 432 747 268 2326
Fragments arranged by size:
2326 747 432 268
AflIII A'CryG_T
Cuts at: 1125 2229 3206 1125
Size: 1104 977 1692
Fragments arranged by size:
1692 1104 977
AgeI A'CCGG_T
Cuts at: 2161 2161
Size: 3773
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 307 307
Size: 3773
AlwNI CAG_nnn'CTG
Cuts at: 1078 2645 1078
Size: 1567 2206
ApaI G_GGCC'C
Cuts at: 145 532 145
Size: 387 3386
ApaLI G'TGCA_C
Cuts at: 2543 2543
Size: 3773
ApoI r'AATT_y
Cuts at: 777 3126 3382 777
Size: 2349 256 1168
Fragments arranged by size:
2349 1168 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3773
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3773
AvaI C'yCGr_G
Cuts at: 337 764 793 337
Size: 427 29 3317
Fragments arranged by size:
3317 427 29
BamHI G'GATC_C
Cuts at: 798 798
Size: 3773
BanI G'GyrC_C
Cuts at: 134 418 789 1203 1451 1486 134
Size: 284 371 414 248 35 2421
Fragments arranged by size:
2421 414 371 284 248 35
BanII G_rGCy'C
Cuts at: 145 187 464 532 638 787 1817 145
Size: 42 277 68 106 149 1030 2101
Fragments arranged by size:
2101 1030 277 149 106 68 42
BbeI G_GCGC'C
Cuts at: 138 1207 1455 138
Size: 1069 248 2456
Fragments arranged by size:
2456 1069 248
BbsI GAAGACnn'nnnn_
Cuts at: 515 515
Size: 3773
BbvCI CC'TCA_GC
Cuts at: 673 673
Size: 3773
BciVI GTATCCnnnnn_n'
Cuts at: 1671 2432 3773 1671
Size: 761 1341 1671
Fragments arranged by size:
1671 1341 761
BclI T'GATC_A
Cuts at: 1293 1293
Size: 3773
BglI GCCn_nnn'nGGC
Cuts at: 652 652
Size: 3773
BglII A'GATC_T
Cuts at: 470 1288 3325 470
Size: 818 2037 918
Fragments arranged by size:
2037 918 818
BlpI GC'TnA_GC
Cuts at: 885 885
Size: 3773
Bme1580I G_kGCm'C
Cuts at: 145 532 1398 1491 2547 145
Size: 387 866 93 1056 1371
Fragments arranged by size:
1371 1056 866 387 93
BmgBI CAC'GTC
Cuts at: 708 708
Size: 3773
BmrI ACTGGGnnnn_n'
Cuts at: 985 1066 1401 3545 985
Size: 81 335 2144 1213
Fragments arranged by size:
2144 1213 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2140 2140
Size: 3773
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 189 685 189
Size: 496 3277
Bpu10I CC'TnA_GC
Cuts at: 673 673
Size: 3773
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 634 949 1828 2320 2618 634
Size: 315 879 492 298 1789
Fragments arranged by size:
1789 879 492 315 298
BsaI GGTCTCn'nnnn_
Cuts at: 377 3477 377
Size: 3100 673
BsaAI yAC'GTr
Cuts at: 1126 1126
Size: 3773
BsaBI GATnn'nnATC
Cuts at: 6 1287 3324 6
Size: 1281 2037 455
Fragments arranged by size:
2037 1281 455
BsaHI Gr'CG_yC
Cuts at: 135 239 584 1204 1452 2154 3650 135
Size: 104 345 620 248 702 1496 258
Fragments arranged by size:
1496 702 620 345 258 248 104
BsaWI w'CCGG_w
Cuts at: 962 1483 2161 2435 2582 2993 962
Size: 521 678 274 147 411 1742
Fragments arranged by size:
1742 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 355 355
Size: 3773
BseYI C'CCAG_C
Cuts at: 193 199 298 414 660 1198 1956 2533 3029
Size: 6 99 116 246 538 758 577 496
Cuts at: 3029 193
Size: 937
Fragments arranged by size:
937 758 577 538 496 246 116 99
6
BsiEI CG_ry'CG
Cuts at: 104 324 618 1315 1361 2569 104
Size: 220 294 697 46 1208 1308
Fragments arranged by size:
1308 1208 697 294 220 46
BsiHKAI G_wGCw'C
Cuts at: 260 464 638 787 1565 1755 2547 260
Size: 204 174 149 778 190 792 1486
Fragments arranged by size:
1486 792 778 204 190 174 149
BsmBI CGTCTCn'nnnn_
Cuts at: 541 541
Size: 3773
Bsp1286I G_dGCh'C
Cuts at: 145 187 260 464 532 638 787 1398 1491
Size: 42 73 204 68 106 149 611 93
Cuts at: 1491 1565 1755 1817 2547 145
Size: 74 190 62 730 1371
Fragments arranged by size:
1371 730 611 204 190 149 106 93
74 73 68 62 42
BspEI T'CCGG_A
Cuts at: 2993 2993
Size: 3773
BspHI T'CATG_A
Cuts at: 2949 2949
Size: 3773
BspMI ACCTGCnnnn'nnnn_
Cuts at: 348 823 1037 1339 1720 348
Size: 475 214 302 381 2401
Fragments arranged by size:
2401 475 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2065 2119 3603 3768 50
Size: 2015 54 1484 165 55
Fragments arranged by size:
2015 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 302 1685 302
Size: 1383 2390
BsrFI r'CCGG_y
Cuts at: 1771 1952 2161 1771
Size: 181 209 3383
Fragments arranged by size:
3383 209 181
BssSI C'ACGA_G
Cuts at: 2044 2402 2044
Size: 358 3415
BstAPI GCAn_nnn'nTGC
Cuts at: 1150 1150
Size: 3773
BstBI TT'CG_AA
Cuts at: 740 2134 740
Size: 1394 2379
BstXI CCAn_nnnn'nTGG
Cuts at: 456 540 456
Size: 84 3689
BstYI r'GATC_y
Cuts at: 1 470 695 798 1265 1288 1623 1869 2881
Size: 469 225 103 467 23 335 246 1012
Cuts at: 2881 2967 2979 3325 1
Size: 86 12 346 449
Fragments arranged by size:
1012 469 467 449 346 335 246 225
103 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 146 146
Size: 3773
BtgI C'CryG_G
Cuts at: 107 107
Size: 3773
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1723 1904 2067 1723
Size: 181 163 3429
Fragments arranged by size:
3429 181 163
BtsI GCAGTG_nn'
Cuts at: 166 1016 166
Size: 850 2923
DraI TTT'AAA
Cuts at: 772 772
Size: 3773
DrdI GACnn_nn'nnGTC
Cuts at: 730 1479 2337 730
Size: 749 858 2166
Fragments arranged by size:
2166 858 749
EaeI y'GGCC_r
Cuts at: 615 1358 1532 1923 1950 3295 615
Size: 743 174 391 27 1345 1093
Fragments arranged by size:
1345 1093 743 391 174 27
EagI C'GGCC_G
Cuts at: 615 1358 615
Size: 743 3030
EarI CTCTTCn'nnn_
Cuts at: 344 737 1796 2006 344
Size: 393 1059 210 2111
Fragments arranged by size:
2111 1059 393 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 506 1981 2291 2437 3583 506
Size: 1475 310 146 1146 696
Fragments arranged by size:
1475 1146 696 310 146
EcoICRI GAG'CTC
Cuts at: 462 636 785 462
Size: 174 149 3450
Fragments arranged by size:
3450 174 149
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 189 685 1598 2030 2777 3045 189
Size: 496 913 432 747 268 917
Fragments arranged by size:
917 913 747 496 432 268
EcoO109I rG'GnC_Cy
Cuts at: 141 142 150 225 274 529 654 912 141
Size: 1 8 75 49 255 125 258 3002
Fragments arranged by size:
3002 258 255 125 75 49 8 1
EcoRI G'AATT_C
Cuts at: 777 777
Size: 3773
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 171 519 1792 171
Size: 348 1273 2152
Fragments arranged by size:
2152 1273 348
FspI TGC'GCA
Cuts at: 1554 1554
Size: 3773
HaeII r_GCGC'y
Cuts at: 138 672 1207 1455 2477 3456 138
Size: 534 535 248 1022 979 455
Fragments arranged by size:
1022 979 535 534 455 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1615 1645 1647 1677 1615
Size: 30 2 30 3711
Fragments arranged by size:
3711 30 30 2
HincII GTy'rAC
Cuts at: 735 812 735
Size: 77 3696
KasI G'GCGC_C
Cuts at: 134 1203 1451 134
Size: 1069 248 2456
Fragments arranged by size:
2456 1069 248
KpnI G_GTAC'C
Cuts at: 793 793
Size: 3773
MluI A'CGCG_T
Cuts at: 3206 3206
Size: 3773
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2444 2628 2444
Size: 184 3589
MscI TGG'CCA
Cuts at: 1534 1534
Size: 3773
MslI CAynn'nnrTG
Cuts at: 454 625 1762 1889 454
Size: 171 1137 127 2338
Fragments arranged by size:
2338 1137 171 127
MspA1I CmG'CkG
Cuts at: 681 884 1198 1558 2571 2816 681
Size: 203 314 360 1013 245 1638
Fragments arranged by size:
1638 1013 360 314 245 203
NaeI GCC'GGC
Cuts at: 1954 1954
Size: 3773
NarI GG'CG_CC
Cuts at: 135 1204 1452 135
Size: 1069 248 2456
Fragments arranged by size:
2456 1069 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3773
NgoMIV G'CCGG_C
Cuts at: 1952 1952
Size: 3773
NspI r_CATG'y
Cuts at: 429 447 826 1326 2233 429
Size: 18 379 500 907 1969
Fragments arranged by size:
1969 907 500 379 18
PasI CC'CwG_GG
Cuts at: 435 435
Size: 3773
PciI A'CATG_T
Cuts at: 2229 2229
Size: 3773
PflMI CCAn_nnn'nTGG
Cuts at: 708 708
Size: 3773
PfoI T'CCnGG_A
Cuts at: 368 569 590 701 368
Size: 201 21 111 3440
Fragments arranged by size:
3440 201 111 21
PmeI GTTT'AAAC
Cuts at: 772 772
Size: 3773
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2912 2944 2912
Size: 32 3741
PpuMI rG'GwC_Cy
Cuts at: 150 225 150
Size: 75 3698
PspOMI G'GGCC_C
Cuts at: 141 528 141
Size: 387 3386
PstI C_TGCA'G
Cuts at: 359 820 1505 359
Size: 461 685 2627
Fragments arranged by size:
2627 685 461
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3773
PvuII CAG'CTG
Cuts at: 1198 1558 1198
Size: 360 3413
RsrII CG'GwC_CG
Cuts at: 1968 1968
Size: 3773
SacI G_AGCT'C
Cuts at: 464 638 787 464
Size: 174 149 3450
Fragments arranged by size:
3450 174 149
SalI G'TCGA_C
Cuts at: 733 810 733
Size: 77 3696
SanDI GG'GwC_CC
Cuts at: 225 225
Size: 3773
SapI GCTCTTCn'nnn_
Cuts at: 344 1796 2006 344
Size: 1452 210 2111
Fragments arranged by size:
2111 1452 210
SbfI CC_TGCA'GG
Cuts at: 820 820
Size: 3773
ScaI AGT'ACT
Cuts at: 3290 3290
Size: 3773
SfcI C'TryA_G
Cuts at: 33 355 816 1501 2494 2685 33
Size: 322 461 685 993 191 1121
Fragments arranged by size:
1121 993 685 461 322 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 652 652
Size: 3773
SfoI GGC'GCC
Cuts at: 136 1205 1453 136
Size: 1069 248 2456
Fragments arranged by size:
2456 1069 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3773
SmaI CCC'GGG
Cuts at: 795 795
Size: 3773
SmlI C'TyrA_G
Cuts at: 649 928 1843 2335 2597 649
Size: 279 915 492 262 1825
Fragments arranged by size:
1825 915 492 279 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3773
SphI G_CATG'C
Cuts at: 429 826 1326 429
Size: 397 500 2876
Fragments arranged by size:
2876 500 397
StyI C'CwwG_G
Cuts at: 107 178 212 907 1261 107
Size: 71 34 695 354 2619
Fragments arranged by size:
2619 695 354 71 34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 328 395 406 1356 1489 1948 2157 328
Size: 67 11 950 133 459 209 1944
Fragments arranged by size:
1944 950 459 209 133 67 11
TatI w'GTAC_w
Cuts at: 3288 3288
Size: 3773
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3290 3322 3290
Size: 32 3741
Tth111I GACn'n_nGTC
Cuts at: 1570 1570
Size: 3773
XbaI T'CTAG_A
Cuts at: 804 804
Size: 3773
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1268 1268
Size: 3773
XmaI C'CCGG_G
Cuts at: 793 793
Size: 3773
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BcgI BfrBI BmtI BsaXI BsgI BsiWI
BsmI BsrGI BssHII BstEII BstZ17I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PmlI PshAI PsiI
PspXI PsrI SacII SexAI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI