Restriction map for
pF1KE0269
(Circular) (Six-base) MAPSORT of: pF1KE0269.seq Check: 9749 from: 1 to: 4412
pF1KE0269 4412 bp
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1163 1163
Size: 4412
AccI GT'mk_AC
Cuts at: 1450 1450
Size: 4412
Acc65I G'GTAC_C
Cuts at: 1428 1428
Size: 4412
AclI AA'CG_TT
Cuts at: 4252 4252
Size: 4412
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2237 2669 3416 3684 2237
Size: 432 747 268 2965
Fragments arranged by size:
2965 747 432 268
AflIII A'CryG_T
Cuts at: 1764 2868 3845 1764
Size: 1104 977 2331
Fragments arranged by size:
2331 1104 977
AgeI A'CCGG_T
Cuts at: 2800 2800
Size: 4412
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 625 1054 625
Size: 429 3983
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 230 262 230
Size: 32 4380
AlwNI CAG_nnn'CTG
Cuts at: 241 622 646 1125 1324 1717 3284 241
Size: 381 24 479 199 393 1567 1369
Fragments arranged by size:
1567 1369 479 393 381 199 24
ApaI G_GGCC'C
Cuts at: 1288 1288
Size: 4412
ApaLI G'TGCA_C
Cuts at: 3182 3182
Size: 4412
ApoI r'AATT_y
Cuts at: 129 888 1416 3765 4021 129
Size: 759 528 2349 256 520
Fragments arranged by size:
2349 759 528 520 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4412
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4412
AvaI C'yCGr_G
Cuts at: 1190 1389 1432 1190
Size: 199 43 4170
Fragments arranged by size:
4170 199 43
AvrII C'CTAG_G
Cuts at: 1276 1276
Size: 4412
BamHI G'GATC_C
Cuts at: 1210 1437 1210
Size: 227 4185
BanI G'GyrC_C
Cuts at: 976 1151 1428 1842 2090 2125 976
Size: 175 277 414 248 35 3263
Fragments arranged by size:
3263 414 277 248 175 35
BanII G_rGCy'C
Cuts at: 1288 1426 2456 1288
Size: 138 1030 3244
Fragments arranged by size:
3244 1030 138
BbeI G_GCGC'C
Cuts at: 1846 2094 1846
Size: 248 4164
BbsI GAAGACnn'nnnn_
Cuts at: 1401 1401
Size: 4412
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4378
BciVI GTATCCnnnnn_n'
Cuts at: 2310 3071 4412 2310
Size: 761 1341 2310
Fragments arranged by size:
2310 1341 761
BclI T'GATC_A
Cuts at: 1932 1932
Size: 4412
BglII A'GATC_T
Cuts at: 1927 3964 1927
Size: 2037 2375
BlpI GC'TnA_GC
Cuts at: 1524 1524
Size: 4412
Bme1580I G_kGCm'C
Cuts at: 955 1288 2037 2130 3186 955
Size: 333 749 93 1056 2181
Fragments arranged by size:
2181 1056 749 333 93
BmrI ACTGGGnnnn_n'
Cuts at: 632 730 1624 1705 2040 4184 632
Size: 98 894 81 335 2144 860
Fragments arranged by size:
2144 894 860 335 98 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 267 622 2779 267
Size: 355 2157 1900
Fragments arranged by size:
2157 1900 355
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1078 1135 1078
Size: 57 4355
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1588 2467 2959 3257 1588
Size: 879 492 298 2743
Fragments arranged by size:
2743 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1241 4116 1241
Size: 2875 1537
BsaAI yAC'GTr
Cuts at: 1765 1765
Size: 4412
BsaBI GATnn'nnATC
Cuts at: 6 386 1926 3963 6
Size: 380 1540 2037 455
Fragments arranged by size:
2037 1540 455 380
BsaHI Gr'CG_yC
Cuts at: 1843 2091 2793 4289 1843
Size: 248 702 1496 1966
Fragments arranged by size:
1966 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1601 2122 2800 3074 3221 3632 1601
Size: 521 678 274 147 411 2381
Fragments arranged by size:
2381 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 203 233 203
Size: 30 4382
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 412 412
Size: 4412
BseYI C'CCAG_C
Cuts at: 997 1139 1281 1837 2595 3172 3668 997
Size: 142 142 556 758 577 496 1741
Fragments arranged by size:
1741 758 577 556 496 142 142
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 955 955
Size: 4412
BsiEI CG_ry'CG
Cuts at: 104 1954 2000 3208 104
Size: 1850 46 1208 1308
Fragments arranged by size:
1850 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1101 1271 1426 2204 2394 3186 1101
Size: 170 155 778 190 792 2327
Fragments arranged by size:
2327 792 778 190 170 155
Bsp1286I G_dGCh'C
Cuts at: 955 1101 1271 1288 1426 2037 2130 2204 2394
Size: 146 170 17 138 611 93 74 190
Cuts at: 2394 2456 3186 955
Size: 62 730 2181
Fragments arranged by size:
2181 730 611 190 170 146 138 93
74 62 17
BspEI T'CCGG_A
Cuts at: 3632 3632
Size: 4412
BspHI T'CATG_A
Cuts at: 557 3588 557
Size: 3031 1381
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1163 1462 1676 1978 2359 1163
Size: 299 214 302 381 3216
Fragments arranged by size:
3216 381 302 299 214
BsrBI CCG'CTC
Cuts at: 50 2704 2758 4242 4407 50
Size: 2654 54 1484 165 55
Fragments arranged by size:
2654 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2324 2324
Size: 4412
BsrFI r'CCGG_y
Cuts at: 1130 1148 2410 2591 2800 1130
Size: 18 1262 181 209 2742
Fragments arranged by size:
2742 1262 209 181 18
BssSI C'ACGA_G
Cuts at: 256 499 2683 3041 256
Size: 243 2184 358 1627
Fragments arranged by size:
2184 1627 358 243
BstAPI GCAn_nnn'nTGC
Cuts at: 646 1789 646
Size: 1143 3269
BstBI TT'CG_AA
Cuts at: 195 2773 195
Size: 2578 1834
BstXI CCAn_nnnn'nTGG
Cuts at: 525 720 525
Size: 195 4217
BstYI r'GATC_y
Cuts at: 1 831 1210 1437 1904 1927 2262 2508 3520
Size: 830 379 227 467 23 335 246 1012
Cuts at: 3520 3606 3618 3964 1
Size: 86 12 346 449
Fragments arranged by size:
1012 830 467 449 379 346 335 246
227 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 372 2362 2543 2706 372
Size: 1990 181 163 2078
Fragments arranged by size:
2078 1990 181 163
BtsI GCAGTG_nn'
Cuts at: 1655 1655
Size: 4412
DraI TTT'AAA
Cuts at: 1411 1411
Size: 4412
DrdI GACnn_nn'nnGTC
Cuts at: 2118 2976 2118
Size: 858 3554
EaeI y'GGCC_r
Cuts at: 527 851 896 962 1341 1997 2171 2562 2589
Size: 324 45 66 379 656 174 391 27
Cuts at: 2589 3934 527
Size: 1345 1005
Fragments arranged by size:
1345 1005 656 391 379 324 174 66
45 27
EagI C'GGCC_G
Cuts at: 1997 1997
Size: 4412
EarI CTCTTCn'nnn_
Cuts at: 341 540 2435 2645 341
Size: 199 1895 210 2108
Fragments arranged by size:
2108 1895 210 199
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2620 2930 3076 4222 2620
Size: 310 146 1146 2810
Fragments arranged by size:
2810 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1424 1424
Size: 4412
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1078 1135 2237 2669 3416 3684 1078
Size: 57 1102 432 747 268 1806
Fragments arranged by size:
1806 1102 747 432 268 57
EcoO109I rG'GnC_Cy
Cuts at: 724 1285 1386 1551 724
Size: 561 101 165 3585
Fragments arranged by size:
3585 561 165 101
EcoRI G'AATT_C
Cuts at: 1416 1416
Size: 4412
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2431 2431
Size: 4412
FspI TGC'GCA
Cuts at: 2193 2193
Size: 4412
HaeII r_GCGC'y
Cuts at: 1846 2094 3116 4095 1846
Size: 248 1022 979 2163
Fragments arranged by size:
2163 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 203 235 2254 2284 2286 2316 203
Size: 32 2019 30 2 30 2299
Fragments arranged by size:
2299 2019 32 30 30 2
HincII GTy'rAC
Cuts at: 299 489 1451 299
Size: 190 962 3260
Fragments arranged by size:
3260 962 190
KasI G'GCGC_C
Cuts at: 1842 2090 1842
Size: 248 4164
KpnI G_GTAC'C
Cuts at: 1432 1432
Size: 4412
MluI A'CGCG_T
Cuts at: 3845 3845
Size: 4412
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 256 884 3083 3267 256
Size: 628 2199 184 1401
Fragments arranged by size:
2199 1401 628 184
MscI TGG'CCA
Cuts at: 529 853 898 964 1343 2173 529
Size: 324 45 66 379 830 2768
Fragments arranged by size:
2768 830 379 324 66 45
MslI CAynn'nnrTG
Cuts at: 311 877 2401 2528 311
Size: 566 1524 127 2195
Fragments arranged by size:
2195 1524 566 127
MspA1I CmG'CkG
Cuts at: 646 1523 1837 2197 3210 3455 646
Size: 877 314 360 1013 245 1603
Fragments arranged by size:
1603 1013 877 360 314 245
NaeI GCC'GGC
Cuts at: 2593 2593
Size: 4412
NarI GG'CG_CC
Cuts at: 1843 2091 1843
Size: 248 4164
NgoMIV G'CCGG_C
Cuts at: 2591 2591
Size: 4412
NspI r_CATG'y
Cuts at: 1465 1965 2872 1465
Size: 500 907 3005
Fragments arranged by size:
3005 907 500
PciI A'CATG_T
Cuts at: 2868 2868
Size: 4412
PmeI GTTT'AAAC
Cuts at: 1411 1411
Size: 4412
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3551 3583 3551
Size: 32 4380
PspOMI G'GGCC_C
Cuts at: 1284 1284
Size: 4412
PstI C_TGCA'G
Cuts at: 1043 1459 2144 1043
Size: 416 685 3311
Fragments arranged by size:
3311 685 416
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4412
PvuII CAG'CTG
Cuts at: 646 1837 2197 646
Size: 1191 360 2861
Fragments arranged by size:
2861 1191 360
RsrII CG'GwC_CG
Cuts at: 2607 2607
Size: 4412
SacI G_AGCT'C
Cuts at: 1426 1426
Size: 4412
SalI G'TCGA_C
Cuts at: 1449 1449
Size: 4412
SapI GCTCTTCn'nnn_
Cuts at: 341 540 2435 2645 341
Size: 199 1895 210 2108
Fragments arranged by size:
2108 1895 210 199
SbfI CC_TGCA'GG
Cuts at: 1459 1459
Size: 4412
ScaI AGT'ACT
Cuts at: 3929 3929
Size: 4412
SfcI C'TryA_G
Cuts at: 33 1039 1455 2140 3133 3324 33
Size: 1006 416 685 993 191 1121
Fragments arranged by size:
1121 1006 993 685 416 191
SfoI GGC'GCC
Cuts at: 1844 2092 1844
Size: 248 4164
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4412
SmaI CCC'GGG
Cuts at: 1192 1391 1434 1192
Size: 199 43 4170
Fragments arranged by size:
4170 199 43
SmlI C'TyrA_G
Cuts at: 1567 2482 2974 3236 1567
Size: 915 492 262 2743
Fragments arranged by size:
2743 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4412
SphI G_CATG'C
Cuts at: 1465 1965 1465
Size: 500 3912
StyI C'CwwG_G
Cuts at: 149 1276 1330 1546 1900 149
Size: 1127 54 216 354 2661
Fragments arranged by size:
2661 1127 354 216 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 972 979 1153 1995 2128 2587 2796 972
Size: 7 174 842 133 459 209 2588
Fragments arranged by size:
2588 842 459 209 174 133 7
TatI w'GTAC_w
Cuts at: 3927 3927
Size: 4412
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1202 1234 3929 3961 1202
Size: 32 2695 32 1653
Fragments arranged by size:
2695 1653 32 32
Tth111I GACn'n_nGTC
Cuts at: 2209 2209
Size: 4412
XbaI T'CTAG_A
Cuts at: 1443 1443
Size: 4412
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 519 1907 519
Size: 1388 3024
XmaI C'CCGG_G
Cuts at: 1190 1389 1432 1190
Size: 199 43 4170
Fragments arranged by size:
4170 199 43
XmnI GAAnn'nnTTC
Cuts at: 690 690
Size: 4412
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PmeI PpiI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AscI BaeI BbvCI
BfrBI BglI BmgBI BmtI Bpu10I BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstZ17I Bsu36I BtgI ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PpuMI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI