(Circular) (Six-base) MAPSORT of: pF1KE0273.seq Check: 5463 from: 1 to: 3422 pF1KE0273 3422 bp With 182 enzymes: SgfI * April 24, 2009 13:12 .. AccI GT'mk_AC Cuts at: 460 460 Size: 3422 Acc65I G'GTAC_C Cuts at: 438 438 Size: 3422 AclI AA'CG_TT Cuts at: 3262 3262 Size: 3422 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1247 1679 2426 2694 1247 Size: 432 747 268 1975 Fragments arranged by size: 1975 747 432 268 AflIII A'CryG_T Cuts at: 774 1878 2855 774 Size: 1104 977 1341 Fragments arranged by size: 1341 1104 977 AgeI A'CCGG_T Cuts at: 1810 1810 Size: 3422 AlwNI CAG_nnn'CTG Cuts at: 727 2294 727 Size: 1567 1855 ApaLI G'TGCA_C Cuts at: 2192 2192 Size: 3422 ApoI r'AATT_y Cuts at: 426 2775 3031 426 Size: 2349 256 817 Fragments arranged by size: 2349 817 256 AseI AT'TA_AT Cuts at: 20 20 Size: 3422 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3422 AvaI C'yCGr_G Cuts at: 442 442 Size: 3422 BamHI G'GATC_C Cuts at: 447 447 Size: 3422 BanI G'GyrC_C Cuts at: 151 438 852 1100 1135 151 Size: 287 414 248 35 2438 Fragments arranged by size: 2438 414 287 248 35 BanII G_rGCy'C Cuts at: 436 1466 436 Size: 1030 2392 BbeI G_GCGC'C Cuts at: 155 856 1104 155 Size: 701 248 2473 Fragments arranged by size: 2473 701 248 BciVI GTATCCnnnnn_n' Cuts at: 1320 2081 3422 1320 Size: 761 1341 1320 Fragments arranged by size: 1341 1320 761 BclI T'GATC_A Cuts at: 942 942 Size: 3422 BglI GCCn_nnn'nGGC Cuts at: 233 233 Size: 3422 BglII A'GATC_T Cuts at: 937 2974 937 Size: 2037 1385 BlpI GC'TnA_GC Cuts at: 534 534 Size: 3422 Bme1580I G_kGCm'C Cuts at: 1047 1140 2196 1047 Size: 93 1056 2273 Fragments arranged by size: 2273 1056 93 BmgBI CAC'GTC Cuts at: 366 366 Size: 3422 BmrI ACTGGGnnnn_n' Cuts at: 634 715 1050 3194 634 Size: 81 335 2144 862 Fragments arranged by size: 2144 862 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1789 1789 Size: 3422 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 364 598 1477 1969 2267 364 Size: 234 879 492 298 1519 Fragments arranged by size: 1519 879 492 298 234 BsaI GGTCTCn'nnnn_ Cuts at: 3126 3126 Size: 3422 BsaAI yAC'GTr Cuts at: 775 775 Size: 3422 BsaBI GATnn'nnATC Cuts at: 6 936 2973 6 Size: 930 2037 455 Fragments arranged by size: 2037 930 455 BsaHI Gr'CG_yC Cuts at: 152 236 853 1101 1803 3299 152 Size: 84 617 248 702 1496 275 Fragments arranged by size: 1496 702 617 275 248 84 BsaWI w'CCGG_w Cuts at: 611 1132 1810 2084 2231 2642 611 Size: 521 678 274 147 411 1391 Fragments arranged by size: 1391 678 521 411 274 147 BseYI C'CCAG_C Cuts at: 847 1605 2182 2678 847 Size: 758 577 496 1591 Fragments arranged by size: 1591 758 577 496 BsiEI CG_ry'CG Cuts at: 104 964 1010 2218 104 Size: 860 46 1208 1308 Fragments arranged by size: 1308 1208 860 46 BsiHKAI G_wGCw'C Cuts at: 436 1214 1404 2196 436 Size: 778 190 792 1662 Fragments arranged by size: 1662 792 778 190 Bsp1286I G_dGCh'C Cuts at: 436 1047 1140 1214 1404 1466 2196 436 Size: 611 93 74 190 62 730 1662 Fragments arranged by size: 1662 730 611 190 93 74 62 BspEI T'CCGG_A Cuts at: 2642 2642 Size: 3422 BspHI T'CATG_A Cuts at: 2598 2598 Size: 3422 BspMI ACCTGCnnnn'nnnn_ Cuts at: 472 686 988 1369 472 Size: 214 302 381 2525 Fragments arranged by size: 2525 381 302 214 BsrBI CCG'CTC Cuts at: 50 1714 1768 3252 3417 50 Size: 1664 54 1484 165 55 Fragments arranged by size: 1664 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1334 1334 Size: 3422 BsrFI r'CCGG_y Cuts at: 1420 1601 1810 1420 Size: 181 209 3032 Fragments arranged by size: 3032 209 181 BssSI C'ACGA_G Cuts at: 1693 2051 1693 Size: 358 3064 BstAPI GCAn_nnn'nTGC Cuts at: 799 799 Size: 3422 BstBI TT'CG_AA Cuts at: 1783 1783 Size: 3422 BstXI CCAn_nnnn'nTGG Cuts at: 366 366 Size: 3422 BstYI r'GATC_y Cuts at: 1 447 914 937 1272 1518 2530 2616 2628 Size: 446 467 23 335 246 1012 86 12 Cuts at: 2628 2974 1 Size: 346 449 Fragments arranged by size: 1012 467 449 446 346 335 246 86 23 12 BtgI C'CryG_G Cuts at: 117 228 359 117 Size: 111 131 3180 Fragments arranged by size: 3180 131 111 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 326 1372 1553 1716 326 Size: 1046 181 163 2032 Fragments arranged by size: 2032 1046 181 163 BtsI GCAGTG_nn' Cuts at: 665 665 Size: 3422 DraI TTT'AAA Cuts at: 421 421 Size: 3422 DrdI GACnn_nn'nnGTC Cuts at: 1128 1986 1128 Size: 858 2564 EaeI y'GGCC_r Cuts at: 114 1007 1181 1572 1599 2944 114 Size: 893 174 391 27 1345 592 Fragments arranged by size: 1345 893 592 391 174 27 EagI C'GGCC_G Cuts at: 1007 1007 Size: 3422 EarI CTCTTCn'nnn_ Cuts at: 1445 1655 1445 Size: 210 3212 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 143 202 1630 1940 2086 3232 143 Size: 59 1428 310 146 1146 333 Fragments arranged by size: 1428 1146 333 310 146 59 EcoICRI GAG'CTC Cuts at: 434 434 Size: 3422 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1247 1679 2426 2694 1247 Size: 432 747 268 1975 Fragments arranged by size: 1975 747 432 268 EcoO109I rG'GnC_Cy Cuts at: 561 561 Size: 3422 EcoRI G'AATT_C Cuts at: 426 426 Size: 3422 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1441 1441 Size: 3422 FspI TGC'GCA Cuts at: 1203 1203 Size: 3422 HaeII r_GCGC'y Cuts at: 155 387 856 1104 2126 3105 155 Size: 232 469 248 1022 979 472 Fragments arranged by size: 1022 979 472 469 248 232 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1264 1294 1296 1326 1264 Size: 30 2 30 3360 Fragments arranged by size: 3360 30 30 2 HincII GTy'rAC Cuts at: 461 461 Size: 3422 KasI G'GCGC_C Cuts at: 151 852 1100 151 Size: 701 248 2473 Fragments arranged by size: 2473 701 248 KpnI G_GTAC'C Cuts at: 442 442 Size: 3422 MluI A'CGCG_T Cuts at: 2855 2855 Size: 3422 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2093 2277 2093 Size: 184 3238 MscI TGG'CCA Cuts at: 1183 1183 Size: 3422 MslI CAynn'nnrTG Cuts at: 358 364 1411 1538 358 Size: 6 1047 127 2242 Fragments arranged by size: 2242 1047 127 6 MspA1I CmG'CkG Cuts at: 119 230 533 847 1207 2220 2465 119 Size: 111 303 314 360 1013 245 1076 Fragments arranged by size: 1076 1013 360 314 303 245 111 NaeI GCC'GGC Cuts at: 1603 1603 Size: 3422 NarI GG'CG_CC Cuts at: 152 853 1101 152 Size: 701 248 2473 Fragments arranged by size: 2473 701 248 NcoI C'CATG_G Cuts at: 359 359 Size: 3422 NgoMIV G'CCGG_C Cuts at: 1601 1601 Size: 3422 NspI r_CATG'y Cuts at: 475 975 1882 475 Size: 500 907 2015 Fragments arranged by size: 2015 907 500 PciI A'CATG_T Cuts at: 1878 1878 Size: 3422 PfoI T'CCnGG_A Cuts at: 293 293 Size: 3422 PmeI GTTT'AAAC Cuts at: 421 421 Size: 3422 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2561 2593 2561 Size: 32 3390 PstI C_TGCA'G Cuts at: 469 1154 469 Size: 685 2737 PvuI CG_AT'CG Cuts at: 104 104 Size: 3422 PvuII CAG'CTG Cuts at: 847 1207 847 Size: 360 3062 RsrII CG'GwC_CG Cuts at: 1617 1617 Size: 3422 SacI G_AGCT'C Cuts at: 436 436 Size: 3422 SacII CC_GC'GG Cuts at: 120 231 120 Size: 111 3311 SalI G'TCGA_C Cuts at: 459 459 Size: 3422 SapI GCTCTTCn'nnn_ Cuts at: 1445 1655 1445 Size: 210 3212 SbfI CC_TGCA'GG Cuts at: 469 469 Size: 3422 ScaI AGT'ACT Cuts at: 2939 2939 Size: 3422 SfcI C'TryA_G Cuts at: 33 465 1150 2143 2334 33 Size: 432 685 993 191 1121 Fragments arranged by size: 1121 993 685 432 191 SfoI GGC'GCC Cuts at: 153 854 1102 153 Size: 701 248 2473 Fragments arranged by size: 2473 701 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3422 SmaI CCC'GGG Cuts at: 444 444 Size: 3422 SmlI C'TyrA_G Cuts at: 379 577 1492 1984 2246 379 Size: 198 915 492 262 1555 Fragments arranged by size: 1555 915 492 262 198 SpeI A'CTAG_T Cuts at: 67 67 Size: 3422 SphI G_CATG'C Cuts at: 475 975 475 Size: 500 2922 StyI C'CwwG_G Cuts at: 189 359 556 910 189 Size: 170 197 354 2701 Fragments arranged by size: 2701 354 197 170 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1005 1138 1597 1806 1005 Size: 133 459 209 2621 Fragments arranged by size: 2621 459 209 133 TatI w'GTAC_w Cuts at: 2937 2937 Size: 3422 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2939 2971 2939 Size: 32 3390 Tth111I GACn'n_nGTC Cuts at: 1219 1219 Size: 3422 XbaI T'CTAG_A Cuts at: 453 453 Size: 3422 XcmI CCAnnnn_n'nnnnTGG Cuts at: 917 917 Size: 3422 XmaI C'CCGG_G Cuts at: 442 442 Size: 3422 XmnI GAAnn'nnTTC Cuts at: 289 289 Size: 3422 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PfoI PmeI PpiI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AlfI AloI ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmtI BpmI Bpu10I BsaXI BseRI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PasI PflMI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI