Restriction map for
pF1KE0277
(Circular) (Six-base) MAPSORT of: pF1KE0277.seq Check: 8887 from: 1 to: 4076
pF1KE0277 4076 bp
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AccI GT'mk_AC
Cuts at: 1114 1114
Size: 4076
Acc65I G'GTAC_C
Cuts at: 1092 1092
Size: 4076
AclI AA'CG_TT
Cuts at: 3916 3916
Size: 4076
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 119 1901 2333 3080 3348 119
Size: 1782 432 747 268 847
Fragments arranged by size:
1782 847 747 432 268
AflII C'TTAA_G
Cuts at: 799 799
Size: 4076
AflIII A'CryG_T
Cuts at: 1428 2532 3509 1428
Size: 1104 977 1995
Fragments arranged by size:
1995 1104 977
AgeI A'CCGG_T
Cuts at: 497 2464 497
Size: 1967 2109
AleI CACnn'nnGTG
Cuts at: 869 913 869
Size: 44 4032
AlwNI CAG_nnn'CTG
Cuts at: 1381 2948 1381
Size: 1567 2509
ApaLI G'TGCA_C
Cuts at: 916 2846 916
Size: 1930 2146
ApoI r'AATT_y
Cuts at: 159 625 1080 3429 3685 159
Size: 466 455 2349 256 550
Fragments arranged by size:
2349 550 466 455 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4076
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4076
AvaI C'yCGr_G
Cuts at: 1096 1096
Size: 4076
BamHI G'GATC_C
Cuts at: 383 1101 383
Size: 718 3358
BanI G'GyrC_C
Cuts at: 1092 1506 1754 1789 1092
Size: 414 248 35 3379
Fragments arranged by size:
3379 414 248 35
BanII G_rGCy'C
Cuts at: 572 1090 2120 572
Size: 518 1030 2528
Fragments arranged by size:
2528 1030 518
BbeI G_GCGC'C
Cuts at: 1510 1758 1510
Size: 248 3828
BciVI GTATCCnnnnn_n'
Cuts at: 1974 2735 4076 1974
Size: 761 1341 1974
Fragments arranged by size:
1974 1341 761
BclI T'GATC_A
Cuts at: 1596 1596
Size: 4076
BglII A'GATC_T
Cuts at: 1591 3628 1591
Size: 2037 2039
BlpI GC'TnA_GC
Cuts at: 693 1188 693
Size: 495 3581
Bme1580I G_kGCm'C
Cuts at: 920 1701 1794 2850 920
Size: 781 93 1056 2146
Fragments arranged by size:
2146 1056 781 93
BmrI ACTGGGnnnn_n'
Cuts at: 426 1288 1369 1704 3848 426
Size: 862 81 335 2144 654
Fragments arranged by size:
2144 862 654 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2443 2443
Size: 4076
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1252 2131 2623 2921 1252
Size: 879 492 298 2407
Fragments arranged by size:
2407 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3780 3780
Size: 4076
BsaAI yAC'GTr
Cuts at: 1429 1429
Size: 4076
BsaBI GATnn'nnATC
Cuts at: 6 1590 3627 6
Size: 1584 2037 455
Fragments arranged by size:
2037 1584 455
BsaHI Gr'CG_yC
Cuts at: 1507 1755 2457 3953 1507
Size: 248 702 1496 1630
Fragments arranged by size:
1630 1496 702 248
BsaWI w'CCGG_w
Cuts at: 497 1265 1786 2464 2738 2885 3296 497
Size: 768 521 678 274 147 411 1277
Fragments arranged by size:
1277 768 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 219 469 219
Size: 250 3826
BseYI C'CCAG_C
Cuts at: 378 1501 2259 2836 3332 378
Size: 1123 758 577 496 1122
Fragments arranged by size:
1123 1122 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 283 658 283
Size: 375 3701
BsiEI CG_ry'CG
Cuts at: 104 1618 1664 2872 104
Size: 1514 46 1208 1308
Fragments arranged by size:
1514 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 920 1090 1868 2058 2850 920
Size: 170 778 190 792 2146
Fragments arranged by size:
2146 792 778 190 170
BsmI GAATG_Cn'
Cuts at: 1046 1046
Size: 4076
Bsp1286I G_dGCh'C
Cuts at: 572 920 1090 1701 1794 1868 2058 2120 2850
Size: 348 170 611 93 74 190 62 730
Cuts at: 2850 572
Size: 1798
Fragments arranged by size:
1798 730 611 348 190 170 93 74
62
BspEI T'CCGG_A
Cuts at: 3296 3296
Size: 4076
BspHI T'CATG_A
Cuts at: 3252 3252
Size: 4076
BspMI ACCTGCnnnn'nnnn_
Cuts at: 862 1126 1340 1642 2023 862
Size: 264 214 302 381 2915
Fragments arranged by size:
2915 381 302 264 214
BsrBI CCG'CTC
Cuts at: 50 2368 2422 3906 4071 50
Size: 2318 54 1484 165 55
Fragments arranged by size:
2318 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 425 503 1988 425
Size: 78 1485 2513
Fragments arranged by size:
2513 1485 78
BsrFI r'CCGG_y
Cuts at: 497 2074 2255 2464 497
Size: 1577 181 209 2109
Fragments arranged by size:
2109 1577 209 181
BsrGI T'GTAC_A
Cuts at: 368 368
Size: 4076
BssSI C'ACGA_G
Cuts at: 2347 2705 2347
Size: 358 3718
BstAPI GCAn_nnn'nTGC
Cuts at: 1453 1453
Size: 4076
BstBI TT'CG_AA
Cuts at: 2437 2437
Size: 4076
BstYI r'GATC_y
Cuts at: 1 383 681 1101 1568 1591 1926 2172 3184
Size: 382 298 420 467 23 335 246 1012
Cuts at: 3184 3270 3282 3628 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 420 382 346 335 298
246 86 23 12
BtgI C'CryG_G
Cuts at: 242 447 944 242
Size: 205 497 3374
Fragments arranged by size:
3374 497 205
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2026 2207 2370 2026
Size: 181 163 3732
Fragments arranged by size:
3732 181 163
BtsI GCAGTG_nn'
Cuts at: 1319 1319
Size: 4076
DraI TTT'AAA
Cuts at: 1075 1075
Size: 4076
DrdI GACnn_nn'nnGTC
Cuts at: 1782 2640 1782
Size: 858 3218
EaeI y'GGCC_r
Cuts at: 1661 1835 2226 2253 3598 1661
Size: 174 391 27 1345 2139
Fragments arranged by size:
2139 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1661 1661
Size: 4076
EarI CTCTTCn'nnn_
Cuts at: 824 2099 2309 824
Size: 1275 210 2591
Fragments arranged by size:
2591 1275 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2284 2594 2740 3886 2284
Size: 310 146 1146 2474
Fragments arranged by size:
2474 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1088 1088
Size: 4076
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 119 1901 2333 3080 3348 119
Size: 1782 432 747 268 847
Fragments arranged by size:
1782 847 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 676 676
Size: 4076
EcoO109I rG'GnC_Cy
Cuts at: 1215 1215
Size: 4076
EcoRI G'AATT_C
Cuts at: 159 1080 159
Size: 921 3155
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2095 2095
Size: 4076
FspI TGC'GCA
Cuts at: 1857 1857
Size: 4076
HaeII r_GCGC'y
Cuts at: 1510 1758 2780 3759 1510
Size: 248 1022 979 1827
Fragments arranged by size:
1827 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1918 1948 1950 1980 1918
Size: 30 2 30 4014
Fragments arranged by size:
4014 30 30 2
HincII GTy'rAC
Cuts at: 1115 1115
Size: 4076
KasI G'GCGC_C
Cuts at: 1506 1754 1506
Size: 248 3828
KpnI G_GTAC'C
Cuts at: 1096 1096
Size: 4076
MfeI C'AATT_G
Cuts at: 258 258
Size: 4076
MluI A'CGCG_T
Cuts at: 3509 3509
Size: 4076
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 740 2747 2931 740
Size: 2007 184 1885
Fragments arranged by size:
2007 1885 184
MscI TGG'CCA
Cuts at: 1837 1837
Size: 4076
MslI CAynn'nnrTG
Cuts at: 869 913 2065 2192 869
Size: 44 1152 127 2753
Fragments arranged by size:
2753 1152 127 44
MspA1I CmG'CkG
Cuts at: 1187 1501 1861 2874 3119 1187
Size: 314 360 1013 245 2144
Fragments arranged by size:
2144 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2257 2257
Size: 4076
NarI GG'CG_CC
Cuts at: 1507 1755 1507
Size: 248 3828
NcoI C'CATG_G
Cuts at: 242 447 242
Size: 205 3871
NgoMIV G'CCGG_C
Cuts at: 2255 2255
Size: 4076
NspI r_CATG'y
Cuts at: 1129 1629 2536 1129
Size: 500 907 2669
Fragments arranged by size:
2669 907 500
PciI A'CATG_T
Cuts at: 2532 2532
Size: 4076
PmeI GTTT'AAAC
Cuts at: 1075 1075
Size: 4076
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3215 3247 3215
Size: 32 4044
PstI C_TGCA'G
Cuts at: 1123 1808 1123
Size: 685 3391
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4076
PvuII CAG'CTG
Cuts at: 1501 1861 1501
Size: 360 3716
RsrII CG'GwC_CG
Cuts at: 2271 2271
Size: 4076
SacI G_AGCT'C
Cuts at: 1090 1090
Size: 4076
SalI G'TCGA_C
Cuts at: 1113 1113
Size: 4076
SapI GCTCTTCn'nnn_
Cuts at: 2099 2309 2099
Size: 210 3866
SbfI CC_TGCA'GG
Cuts at: 1123 1123
Size: 4076
ScaI AGT'ACT
Cuts at: 3593 3593
Size: 4076
SfcI C'TryA_G
Cuts at: 33 1008 1119 1804 2797 2988 33
Size: 975 111 685 993 191 1121
Fragments arranged by size:
1121 993 975 685 191 111
SfoI GGC'GCC
Cuts at: 1508 1756 1508
Size: 248 3828
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4076
SmaI CCC'GGG
Cuts at: 1098 1098
Size: 4076
SmlI C'TyrA_G
Cuts at: 799 1231 2146 2638 2900 799
Size: 432 915 492 262 1975
Fragments arranged by size:
1975 915 492 432 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4076
SphI G_CATG'C
Cuts at: 1129 1629 1129
Size: 500 3576
StyI C'CwwG_G
Cuts at: 242 447 1210 1564 242
Size: 205 763 354 2754
Fragments arranged by size:
2754 763 354 205
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1659 1792 2251 2460 1659
Size: 133 459 209 3275
Fragments arranged by size:
3275 459 209 133
TatI w'GTAC_w
Cuts at: 368 3591 368
Size: 3223 853
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3593 3625 3593
Size: 32 4044
Tth111I GACn'n_nGTC
Cuts at: 1873 1873
Size: 4076
XbaI T'CTAG_A
Cuts at: 1107 1107
Size: 4076
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 249 958 1571 249
Size: 709 613 2754
Fragments arranged by size:
2754 709 613
XmaI C'CCGG_G
Cuts at: 1096 1096
Size: 4076
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AlfI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BsaXI BsiWI BsmBI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI