Restriction map for
pF1KE0279
(Circular) (Six-base) MAPSORT of: pF1KE0279.seq Check: 8054 from: 1 to: 3737
pF1KE0279 3737 bp
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AccI GT'mk_AC
Cuts at: 380 599 775 380
Size: 219 176 3342
Fragments arranged by size:
3342 219 176
Acc65I G'GTAC_C
Cuts at: 753 753
Size: 3737
AclI AA'CG_TT
Cuts at: 3577 3577
Size: 3737
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1562 1994 2741 3009 1562
Size: 432 747 268 2290
Fragments arranged by size:
2290 747 432 268
AflIII A'CryG_T
Cuts at: 1089 2193 3170 1089
Size: 1104 977 1656
Fragments arranged by size:
1656 1104 977
AgeI A'CCGG_T
Cuts at: 2125 2125
Size: 3737
AhdI GACnn_n'nnGTC
Cuts at: 456 456
Size: 3737
AlwNI CAG_nnn'CTG
Cuts at: 1042 2609 1042
Size: 1567 2170
ApaLI G'TGCA_C
Cuts at: 2507 2507
Size: 3737
ApoI r'AATT_y
Cuts at: 325 741 3090 3346 325
Size: 416 2349 256 716
Fragments arranged by size:
2349 716 416 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3737
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3737
AvaI C'yCGr_G
Cuts at: 757 757
Size: 3737
BamHI G'GATC_C
Cuts at: 762 762
Size: 3737
BanI G'GyrC_C
Cuts at: 703 753 1167 1415 1450 703
Size: 50 414 248 35 2990
Fragments arranged by size:
2990 414 248 50 35
BanII G_rGCy'C
Cuts at: 751 1781 751
Size: 1030 2707
BbeI G_GCGC'C
Cuts at: 1171 1419 1171
Size: 248 3489
BbsI GAAGACnn'nnnn_
Cuts at: 254 254
Size: 3737
BciVI GTATCCnnnnn_n'
Cuts at: 1635 2396 3737 1635
Size: 761 1341 1635
Fragments arranged by size:
1635 1341 761
BclI T'GATC_A
Cuts at: 1257 1257
Size: 3737
BglII A'GATC_T
Cuts at: 215 1252 3289 215
Size: 1037 2037 663
Fragments arranged by size:
2037 1037 663
BlpI GC'TnA_GC
Cuts at: 849 849
Size: 3737
Bme1580I G_kGCm'C
Cuts at: 192 1362 1455 2511 192
Size: 1170 93 1056 1418
Fragments arranged by size:
1418 1170 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 122 949 1030 1365 3509 122
Size: 827 81 335 2144 350
Fragments arranged by size:
2144 827 350 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2104 2104
Size: 3737
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 525 913 1792 2284 2582 525
Size: 388 879 492 298 1680
Fragments arranged by size:
1680 879 492 388 298
BsaI GGTCTCn'nnnn_
Cuts at: 683 3441 683
Size: 2758 979
BsaAI yAC'GTr
Cuts at: 414 1090 414
Size: 676 3061
BsaBI GATnn'nnATC
Cuts at: 6 1251 3288 6
Size: 1245 2037 455
Fragments arranged by size:
2037 1245 455
BsaHI Gr'CG_yC
Cuts at: 1168 1416 2118 3614 1168
Size: 248 702 1496 1291
Fragments arranged by size:
1496 1291 702 248
BsaWI w'CCGG_w
Cuts at: 645 926 1447 2125 2399 2546 2957 645
Size: 281 521 678 274 147 411 1425
Fragments arranged by size:
1425 678 521 411 281 274 147
BseYI C'CCAG_C
Cuts at: 313 1162 1920 2497 2993 313
Size: 849 758 577 496 1057
Fragments arranged by size:
1057 849 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1279 1325 2533 104
Size: 1175 46 1208 1308
Fragments arranged by size:
1308 1208 1175 46
BsiHKAI G_wGCw'C
Cuts at: 413 751 1529 1719 2511 413
Size: 338 778 190 792 1639
Fragments arranged by size:
1639 792 778 338 190
Bsp1286I G_dGCh'C
Cuts at: 192 413 751 1362 1455 1529 1719 1781 2511
Size: 221 338 611 93 74 190 62 730
Cuts at: 2511 192
Size: 1418
Fragments arranged by size:
1418 730 611 338 221 190 93 74
62
BspEI T'CCGG_A
Cuts at: 645 2957 645
Size: 2312 1425
BspHI T'CATG_A
Cuts at: 2913 2913
Size: 3737
BspMI ACCTGCnnnn'nnnn_
Cuts at: 787 1001 1303 1684 787
Size: 214 302 381 2840
Fragments arranged by size:
2840 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2029 2083 3567 3732 50
Size: 1979 54 1484 165 55
Fragments arranged by size:
1979 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 724 1649 724
Size: 925 2812
BsrFI r'CCGG_y
Cuts at: 1735 1916 2125 1735
Size: 181 209 3347
Fragments arranged by size:
3347 209 181
BssSI C'ACGA_G
Cuts at: 475 2008 2366 475
Size: 1533 358 1846
Fragments arranged by size:
1846 1533 358
BstAPI GCAn_nnn'nTGC
Cuts at: 351 1114 351
Size: 763 2974
BstBI TT'CG_AA
Cuts at: 2098 2098
Size: 3737
BstYI r'GATC_y
Cuts at: 1 215 762 1229 1252 1587 1833 2845 2931
Size: 214 547 467 23 335 246 1012 86
Cuts at: 2931 2943 3289 1
Size: 12 346 449
Fragments arranged by size:
1012 547 467 449 346 335 246 214
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3737
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1687 1868 2031 1687
Size: 181 163 3393
Fragments arranged by size:
3393 181 163
BtsI GCAGTG_nn'
Cuts at: 980 980
Size: 3737
DraI TTT'AAA
Cuts at: 736 736
Size: 3737
DrdI GACnn_nn'nnGTC
Cuts at: 1443 2301 1443
Size: 858 2879
EaeI y'GGCC_r
Cuts at: 479 1322 1496 1887 1914 3259 479
Size: 843 174 391 27 1345 957
Fragments arranged by size:
1345 957 843 391 174 27
EagI C'GGCC_G
Cuts at: 1322 1322
Size: 3737
EarI CTCTTCn'nnn_
Cuts at: 1760 1970 1760
Size: 210 3527
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1945 2255 2401 3547 1945
Size: 310 146 1146 2135
Fragments arranged by size:
2135 1146 310 146
EcoICRI GAG'CTC
Cuts at: 749 749
Size: 3737
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1562 1994 2741 3009 1562
Size: 432 747 268 2290
Fragments arranged by size:
2290 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 582 876 582
Size: 294 3443
EcoRI G'AATT_C
Cuts at: 325 741 325
Size: 416 3321
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 420 1756 420
Size: 1336 2401
FspI TGC'GCA
Cuts at: 355 1518 355
Size: 1163 2574
FspAI rTGC'GCAy
Cuts at: 355 355
Size: 3737
HaeII r_GCGC'y
Cuts at: 122 335 1171 1419 2441 3420 122
Size: 213 836 248 1022 979 439
Fragments arranged by size:
1022 979 836 439 248 213
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 264 296 442 474 1579 1609 1611 1641 264
Size: 32 146 32 1105 30 2 30 2360
Fragments arranged by size:
2360 1105 146 32 32 30 30 2
HincII GTy'rAC
Cuts at: 600 776 600
Size: 176 3561
KasI G'GCGC_C
Cuts at: 1167 1415 1167
Size: 248 3489
KpnI G_GTAC'C
Cuts at: 757 757
Size: 3737
MluI A'CGCG_T
Cuts at: 3170 3170
Size: 3737
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2408 2592 2408
Size: 184 3553
MscI TGG'CCA
Cuts at: 481 1498 481
Size: 1017 2720
MslI CAynn'nnrTG
Cuts at: 1726 1853 1726
Size: 127 3610
MspA1I CmG'CkG
Cuts at: 313 848 1162 1522 2535 2780 313
Size: 535 314 360 1013 245 1270
Fragments arranged by size:
1270 1013 535 360 314 245
NaeI GCC'GGC
Cuts at: 1918 1918
Size: 3737
NarI GG'CG_CC
Cuts at: 1168 1416 1168
Size: 248 3489
NcoI C'CATG_G
Cuts at: 107 107
Size: 3737
NgoMIV G'CCGG_C
Cuts at: 1916 1916
Size: 3737
NspI r_CATG'y
Cuts at: 354 790 1290 2197 354
Size: 436 500 907 1894
Fragments arranged by size:
1894 907 500 436
PciI A'CATG_T
Cuts at: 2193 2193
Size: 3737
PmeI GTTT'AAAC
Cuts at: 736 736
Size: 3737
PmlI CAC'GTG
Cuts at: 414 414
Size: 3737
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2876 2908 2876
Size: 32 3705
PpuMI rG'GwC_Cy
Cuts at: 582 582
Size: 3737
PstI C_TGCA'G
Cuts at: 784 1469 784
Size: 685 3052
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3737
PvuII CAG'CTG
Cuts at: 313 1162 1522 313
Size: 849 360 2528
Fragments arranged by size:
2528 849 360
RsrII CG'GwC_CG
Cuts at: 1932 1932
Size: 3737
SacI G_AGCT'C
Cuts at: 751 751
Size: 3737
SalI G'TCGA_C
Cuts at: 598 774 598
Size: 176 3561
SapI GCTCTTCn'nnn_
Cuts at: 1760 1970 1760
Size: 210 3527
SbfI CC_TGCA'GG
Cuts at: 784 784
Size: 3737
ScaI AGT'ACT
Cuts at: 3254 3254
Size: 3737
SfcI C'TryA_G
Cuts at: 33 780 1465 2458 2649 33
Size: 747 685 993 191 1121
Fragments arranged by size:
1121 993 747 685 191
SfoI GGC'GCC
Cuts at: 1169 1417 1169
Size: 248 3489
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3737
SmaI CCC'GGG
Cuts at: 759 759
Size: 3737
SmlI C'TyrA_G
Cuts at: 504 892 1807 2299 2561 504
Size: 388 915 492 262 1680
Fragments arranged by size:
1680 915 492 388 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3737
SphI G_CATG'C
Cuts at: 790 1290 790
Size: 500 3237
SspI AAT'ATT
Cuts at: 555 555
Size: 3737
StyI C'CwwG_G
Cuts at: 107 578 871 1225 107
Size: 471 293 354 2619
Fragments arranged by size:
2619 471 354 293
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 721 1320 1453 1912 2121 721
Size: 599 133 459 209 2337
Fragments arranged by size:
2337 599 459 209 133
TatI w'GTAC_w
Cuts at: 3252 3252
Size: 3737
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3254 3286 3254
Size: 32 3705
Tth111I GACn'n_nGTC
Cuts at: 1534 1534
Size: 3737
XbaI T'CTAG_A
Cuts at: 768 768
Size: 3737
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 150 1232 150
Size: 1082 2655
XmaI C'CCGG_G
Cuts at: 757 757
Size: 3737
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PmeI PmlI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BsaXI BseRI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI
XmnI ZraI