(Circular) (Six-base) MAPSORT of: pF1KE0279.seq Check: 8054 from: 1 to: 3737 pF1KE0279 3737 bp With 182 enzymes: SgfI * April 24, 2009 13:12 .. AccI GT'mk_AC Cuts at: 380 599 775 380 Size: 219 176 3342 Fragments arranged by size: 3342 219 176 Acc65I G'GTAC_C Cuts at: 753 753 Size: 3737 AclI AA'CG_TT Cuts at: 3577 3577 Size: 3737 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1562 1994 2741 3009 1562 Size: 432 747 268 2290 Fragments arranged by size: 2290 747 432 268 AflIII A'CryG_T Cuts at: 1089 2193 3170 1089 Size: 1104 977 1656 Fragments arranged by size: 1656 1104 977 AgeI A'CCGG_T Cuts at: 2125 2125 Size: 3737 AhdI GACnn_n'nnGTC Cuts at: 456 456 Size: 3737 AlwNI CAG_nnn'CTG Cuts at: 1042 2609 1042 Size: 1567 2170 ApaLI G'TGCA_C Cuts at: 2507 2507 Size: 3737 ApoI r'AATT_y Cuts at: 325 741 3090 3346 325 Size: 416 2349 256 716 Fragments arranged by size: 2349 716 416 256 AseI AT'TA_AT Cuts at: 20 20 Size: 3737 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3737 AvaI C'yCGr_G Cuts at: 757 757 Size: 3737 BamHI G'GATC_C Cuts at: 762 762 Size: 3737 BanI G'GyrC_C Cuts at: 703 753 1167 1415 1450 703 Size: 50 414 248 35 2990 Fragments arranged by size: 2990 414 248 50 35 BanII G_rGCy'C Cuts at: 751 1781 751 Size: 1030 2707 BbeI G_GCGC'C Cuts at: 1171 1419 1171 Size: 248 3489 BbsI GAAGACnn'nnnn_ Cuts at: 254 254 Size: 3737 BciVI GTATCCnnnnn_n' Cuts at: 1635 2396 3737 1635 Size: 761 1341 1635 Fragments arranged by size: 1635 1341 761 BclI T'GATC_A Cuts at: 1257 1257 Size: 3737 BglII A'GATC_T Cuts at: 215 1252 3289 215 Size: 1037 2037 663 Fragments arranged by size: 2037 1037 663 BlpI GC'TnA_GC Cuts at: 849 849 Size: 3737 Bme1580I G_kGCm'C Cuts at: 192 1362 1455 2511 192 Size: 1170 93 1056 1418 Fragments arranged by size: 1418 1170 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 122 949 1030 1365 3509 122 Size: 827 81 335 2144 350 Fragments arranged by size: 2144 827 350 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2104 2104 Size: 3737 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 525 913 1792 2284 2582 525 Size: 388 879 492 298 1680 Fragments arranged by size: 1680 879 492 388 298 BsaI GGTCTCn'nnnn_ Cuts at: 683 3441 683 Size: 2758 979 BsaAI yAC'GTr Cuts at: 414 1090 414 Size: 676 3061 BsaBI GATnn'nnATC Cuts at: 6 1251 3288 6 Size: 1245 2037 455 Fragments arranged by size: 2037 1245 455 BsaHI Gr'CG_yC Cuts at: 1168 1416 2118 3614 1168 Size: 248 702 1496 1291 Fragments arranged by size: 1496 1291 702 248 BsaWI w'CCGG_w Cuts at: 645 926 1447 2125 2399 2546 2957 645 Size: 281 521 678 274 147 411 1425 Fragments arranged by size: 1425 678 521 411 281 274 147 BseYI C'CCAG_C Cuts at: 313 1162 1920 2497 2993 313 Size: 849 758 577 496 1057 Fragments arranged by size: 1057 849 758 577 496 BsiEI CG_ry'CG Cuts at: 104 1279 1325 2533 104 Size: 1175 46 1208 1308 Fragments arranged by size: 1308 1208 1175 46 BsiHKAI G_wGCw'C Cuts at: 413 751 1529 1719 2511 413 Size: 338 778 190 792 1639 Fragments arranged by size: 1639 792 778 338 190 Bsp1286I G_dGCh'C Cuts at: 192 413 751 1362 1455 1529 1719 1781 2511 Size: 221 338 611 93 74 190 62 730 Cuts at: 2511 192 Size: 1418 Fragments arranged by size: 1418 730 611 338 221 190 93 74 62 BspEI T'CCGG_A Cuts at: 645 2957 645 Size: 2312 1425 BspHI T'CATG_A Cuts at: 2913 2913 Size: 3737 BspMI ACCTGCnnnn'nnnn_ Cuts at: 787 1001 1303 1684 787 Size: 214 302 381 2840 Fragments arranged by size: 2840 381 302 214 BsrBI CCG'CTC Cuts at: 50 2029 2083 3567 3732 50 Size: 1979 54 1484 165 55 Fragments arranged by size: 1979 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 724 1649 724 Size: 925 2812 BsrFI r'CCGG_y Cuts at: 1735 1916 2125 1735 Size: 181 209 3347 Fragments arranged by size: 3347 209 181 BssSI C'ACGA_G Cuts at: 475 2008 2366 475 Size: 1533 358 1846 Fragments arranged by size: 1846 1533 358 BstAPI GCAn_nnn'nTGC Cuts at: 351 1114 351 Size: 763 2974 BstBI TT'CG_AA Cuts at: 2098 2098 Size: 3737 BstYI r'GATC_y Cuts at: 1 215 762 1229 1252 1587 1833 2845 2931 Size: 214 547 467 23 335 246 1012 86 Cuts at: 2931 2943 3289 1 Size: 12 346 449 Fragments arranged by size: 1012 547 467 449 346 335 246 214 86 23 12 BtgI C'CryG_G Cuts at: 107 107 Size: 3737 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1687 1868 2031 1687 Size: 181 163 3393 Fragments arranged by size: 3393 181 163 BtsI GCAGTG_nn' Cuts at: 980 980 Size: 3737 DraI TTT'AAA Cuts at: 736 736 Size: 3737 DrdI GACnn_nn'nnGTC Cuts at: 1443 2301 1443 Size: 858 2879 EaeI y'GGCC_r Cuts at: 479 1322 1496 1887 1914 3259 479 Size: 843 174 391 27 1345 957 Fragments arranged by size: 1345 957 843 391 174 27 EagI C'GGCC_G Cuts at: 1322 1322 Size: 3737 EarI CTCTTCn'nnn_ Cuts at: 1760 1970 1760 Size: 210 3527 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1945 2255 2401 3547 1945 Size: 310 146 1146 2135 Fragments arranged by size: 2135 1146 310 146 EcoICRI GAG'CTC Cuts at: 749 749 Size: 3737 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1562 1994 2741 3009 1562 Size: 432 747 268 2290 Fragments arranged by size: 2290 747 432 268 EcoO109I rG'GnC_Cy Cuts at: 582 876 582 Size: 294 3443 EcoRI G'AATT_C Cuts at: 325 741 325 Size: 416 3321 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 420 1756 420 Size: 1336 2401 FspI TGC'GCA Cuts at: 355 1518 355 Size: 1163 2574 FspAI rTGC'GCAy Cuts at: 355 355 Size: 3737 HaeII r_GCGC'y Cuts at: 122 335 1171 1419 2441 3420 122 Size: 213 836 248 1022 979 439 Fragments arranged by size: 1022 979 836 439 248 213 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 264 296 442 474 1579 1609 1611 1641 264 Size: 32 146 32 1105 30 2 30 2360 Fragments arranged by size: 2360 1105 146 32 32 30 30 2 HincII GTy'rAC Cuts at: 600 776 600 Size: 176 3561 KasI G'GCGC_C Cuts at: 1167 1415 1167 Size: 248 3489 KpnI G_GTAC'C Cuts at: 757 757 Size: 3737 MluI A'CGCG_T Cuts at: 3170 3170 Size: 3737 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2408 2592 2408 Size: 184 3553 MscI TGG'CCA Cuts at: 481 1498 481 Size: 1017 2720 MslI CAynn'nnrTG Cuts at: 1726 1853 1726 Size: 127 3610 MspA1I CmG'CkG Cuts at: 313 848 1162 1522 2535 2780 313 Size: 535 314 360 1013 245 1270 Fragments arranged by size: 1270 1013 535 360 314 245 NaeI GCC'GGC Cuts at: 1918 1918 Size: 3737 NarI GG'CG_CC Cuts at: 1168 1416 1168 Size: 248 3489 NcoI C'CATG_G Cuts at: 107 107 Size: 3737 NgoMIV G'CCGG_C Cuts at: 1916 1916 Size: 3737 NspI r_CATG'y Cuts at: 354 790 1290 2197 354 Size: 436 500 907 1894 Fragments arranged by size: 1894 907 500 436 PciI A'CATG_T Cuts at: 2193 2193 Size: 3737 PmeI GTTT'AAAC Cuts at: 736 736 Size: 3737 PmlI CAC'GTG Cuts at: 414 414 Size: 3737 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2876 2908 2876 Size: 32 3705 PpuMI rG'GwC_Cy Cuts at: 582 582 Size: 3737 PstI C_TGCA'G Cuts at: 784 1469 784 Size: 685 3052 PvuI CG_AT'CG Cuts at: 104 104 Size: 3737 PvuII CAG'CTG Cuts at: 313 1162 1522 313 Size: 849 360 2528 Fragments arranged by size: 2528 849 360 RsrII CG'GwC_CG Cuts at: 1932 1932 Size: 3737 SacI G_AGCT'C Cuts at: 751 751 Size: 3737 SalI G'TCGA_C Cuts at: 598 774 598 Size: 176 3561 SapI GCTCTTCn'nnn_ Cuts at: 1760 1970 1760 Size: 210 3527 SbfI CC_TGCA'GG Cuts at: 784 784 Size: 3737 ScaI AGT'ACT Cuts at: 3254 3254 Size: 3737 SfcI C'TryA_G Cuts at: 33 780 1465 2458 2649 33 Size: 747 685 993 191 1121 Fragments arranged by size: 1121 993 747 685 191 SfoI GGC'GCC Cuts at: 1169 1417 1169 Size: 248 3489 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3737 SmaI CCC'GGG Cuts at: 759 759 Size: 3737 SmlI C'TyrA_G Cuts at: 504 892 1807 2299 2561 504 Size: 388 915 492 262 1680 Fragments arranged by size: 1680 915 492 388 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 3737 SphI G_CATG'C Cuts at: 790 1290 790 Size: 500 3237 SspI AAT'ATT Cuts at: 555 555 Size: 3737 StyI C'CwwG_G Cuts at: 107 578 871 1225 107 Size: 471 293 354 2619 Fragments arranged by size: 2619 471 354 293 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 721 1320 1453 1912 2121 721 Size: 599 133 459 209 2337 Fragments arranged by size: 2337 599 459 209 133 TatI w'GTAC_w Cuts at: 3252 3252 Size: 3737 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3254 3286 3254 Size: 32 3705 Tth111I GACn'n_nGTC Cuts at: 1534 1534 Size: 3737 XbaI T'CTAG_A Cuts at: 768 768 Size: 3737 XcmI CCAnnnn_n'nnnnTGG Cuts at: 150 1232 150 Size: 1082 2655 XmaI C'CCGG_G Cuts at: 757 757 Size: 3737 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI PmlI PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AleI AlfI AloI ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI BpmI Bpu10I BsaXI BseRI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PasI PflMI PfoI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI XmnI ZraI