Restriction map for
pF1KE0282
(Circular) (Six-base) MAPSORT of: pF1KE0282.seq Check: 6061 from: 1 to: 4409
pF1KE0282 4409 bp
With 182 enzymes: SgfI *
October 18, 2013 09:44 ..
AccI GT'mk_AC
Cuts at: 637 999 1447 637
Size: 362 448 3599
Fragments arranged by size:
3599 448 362
Acc65I G'GTAC_C
Cuts at: 1425 1425
Size: 4409
AclI AA'CG_TT
Cuts at: 4249 4249
Size: 4409
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 572 1403 2234 2666 3413 3681 572
Size: 831 831 432 747 268 1300
Fragments arranged by size:
1300 831 831 747 432 268
AflIII A'CryG_T
Cuts at: 259 1761 2865 3842 259
Size: 1502 1104 977 826
Fragments arranged by size:
1502 1104 977 826
AgeI A'CCGG_T
Cuts at: 2797 2797
Size: 4409
AhdI GACnn_n'nnGTC
Cuts at: 1395 1395
Size: 4409
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 202 202
Size: 4409
AlwNI CAG_nnn'CTG
Cuts at: 1714 3281 1714
Size: 1567 2842
ApaLI G'TGCA_C
Cuts at: 3179 3179
Size: 4409
ApoI r'AATT_y
Cuts at: 287 491 1413 3762 4018 287
Size: 204 922 2349 256 678
Fragments arranged by size:
2349 922 678 256 204
AseI AT'TA_AT
Cuts at: 20 20
Size: 4409
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4409
AvaI C'yCGr_G
Cuts at: 725 1040 1429 725
Size: 315 389 3705
Fragments arranged by size:
3705 389 315
BamHI G'GATC_C
Cuts at: 1434 1434
Size: 4409
BanI G'GyrC_C
Cuts at: 1425 1839 2087 2122 1425
Size: 414 248 35 3712
Fragments arranged by size:
3712 414 248 35
BanII G_rGCy'C
Cuts at: 328 960 1423 2453 328
Size: 632 463 1030 2284
Fragments arranged by size:
2284 1030 632 463
BbeI G_GCGC'C
Cuts at: 1843 2091 1843
Size: 248 4161
BbsI GAAGACnn'nnnn_
Cuts at: 443 1316 1376 443
Size: 873 60 3476
Fragments arranged by size:
3476 873 60
BciVI GTATCCnnnnn_n'
Cuts at: 2307 3068 4409 2307
Size: 761 1341 2307
Fragments arranged by size:
2307 1341 761
BclI T'GATC_A
Cuts at: 1929 1929
Size: 4409
BglI GCCn_nnn'nGGC
Cuts at: 473 473
Size: 4409
BglII A'GATC_T
Cuts at: 1138 1924 3961 1138
Size: 786 2037 1586
Fragments arranged by size:
2037 1586 786
BlpI GC'TnA_GC
Cuts at: 1521 1521
Size: 4409
Bme1580I G_kGCm'C
Cuts at: 2034 2127 3183 2034
Size: 93 1056 3260
Fragments arranged by size:
3260 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 714 1621 1702 2037 4181 714
Size: 907 81 335 2144 942
Fragments arranged by size:
2144 942 907 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 342 2776 342
Size: 2434 1975
Bpu10I CC'TnA_GC
Cuts at: 622 622
Size: 4409
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 990 1585 2464 2956 3254 990
Size: 595 879 492 298 2145
Fragments arranged by size:
2145 879 595 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1043 4113 1043
Size: 3070 1339
BsaAI yAC'GTr
Cuts at: 1762 1762
Size: 4409
BsaBI GATnn'nnATC
Cuts at: 6 299 1923 3960 6
Size: 293 1624 2037 455
Fragments arranged by size:
2037 1624 455 293
BsaHI Gr'CG_yC
Cuts at: 1840 2088 2790 4286 1840
Size: 248 702 1496 1963
Fragments arranged by size:
1963 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1285 1598 2119 2797 3071 3218 3629 1285
Size: 313 521 678 274 147 411 2065
Fragments arranged by size:
2065 678 521 411 313 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1071 1101 1191 1221 1071
Size: 30 90 30 4259
Fragments arranged by size:
4259 90 30 30
BseYI C'CCAG_C
Cuts at: 1834 2592 3169 3665 1834
Size: 758 577 496 2578
Fragments arranged by size:
2578 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 641 641
Size: 4409
BsiEI CG_ry'CG
Cuts at: 104 119 1951 1997 3205 104
Size: 15 1832 46 1208 1308
Fragments arranged by size:
1832 1308 1208 46 15
BsiHKAI G_wGCw'C
Cuts at: 328 1423 2201 2391 3183 328
Size: 1095 778 190 792 1554
Fragments arranged by size:
1554 1095 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 328 960 1423 2034 2127 2201 2391 2453 3183
Size: 632 463 611 93 74 190 62 730
Cuts at: 3183 328
Size: 1554
Fragments arranged by size:
1554 730 632 611 463 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3629 3629
Size: 4409
BspHI T'CATG_A
Cuts at: 291 335 3585 291
Size: 44 3250 1115
Fragments arranged by size:
3250 1115 44
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1025 1459 1673 1975 2356 1025
Size: 434 214 302 381 3078
Fragments arranged by size:
3078 434 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2701 2755 4239 4404 50
Size: 2651 54 1484 165 55
Fragments arranged by size:
2651 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2321 2321
Size: 4409
BsrFI r'CCGG_y
Cuts at: 2407 2588 2797 2407
Size: 181 209 4019
Fragments arranged by size:
4019 209 181
BssSI C'ACGA_G
Cuts at: 199 959 2680 3038 199
Size: 760 1721 358 1570
Fragments arranged by size:
1721 1570 760 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1786 1786
Size: 4409
BstBI TT'CG_AA
Cuts at: 2770 2770
Size: 4409
BstYI r'GATC_y
Cuts at: 1 1138 1255 1434 1901 1924 2259 2505 3517
Size: 1137 117 179 467 23 335 246 1012
Cuts at: 3517 3603 3615 3961 1
Size: 86 12 346 449
Fragments arranged by size:
1137 1012 467 449 346 335 246 179
117 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2359 2540 2703 2359
Size: 181 163 4065
Fragments arranged by size:
4065 181 163
BtsI GCAGTG_nn'
Cuts at: 1652 1652
Size: 4409
ClaI AT'CG_AT
Cuts at: 1119 1119
Size: 4409
DraI TTT'AAA
Cuts at: 1408 1408
Size: 4409
DrdI GACnn_nn'nnGTC
Cuts at: 2115 2973 2115
Size: 858 3551
EaeI y'GGCC_r
Cuts at: 116 1994 2168 2559 2586 3931 116
Size: 1878 174 391 27 1345 594
Fragments arranged by size:
1878 1345 594 391 174 27
EagI C'GGCC_G
Cuts at: 116 1994 116
Size: 1878 2531
EarI CTCTTCn'nnn_
Cuts at: 316 602 926 2432 2642 316
Size: 286 324 1506 210 2083
Fragments arranged by size:
2083 1506 324 286 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1092 2617 2927 3073 4219 1092
Size: 1525 310 146 1146 1282
Fragments arranged by size:
1525 1282 1146 310 146
EcoICRI GAG'CTC
Cuts at: 326 1421 326
Size: 1095 3314
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 572 1403 2234 2666 3413 3681 572
Size: 831 831 432 747 268 1300
Fragments arranged by size:
1300 831 831 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1548 1548
Size: 4409
EcoRI G'AATT_C
Cuts at: 1413 1413
Size: 4409
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2428 2428
Size: 4409
FspI TGC'GCA
Cuts at: 2190 2190
Size: 4409
HaeII r_GCGC'y
Cuts at: 1843 2091 3113 4092 1843
Size: 248 1022 979 2160
Fragments arranged by size:
2160 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 706 738 1168 1191 1200 1216 1223 1248 1363
Size: 32 430 23 9 16 7 25 115
Cuts at: 1363 1381 1395 1413 2251 2281 2283 2313 706
Size: 18 14 18 838 30 2 30 2802
Fragments arranged by size:
2802 838 430 115 32 30 30 25
23 18 18 16 14 9 7 2
HincII GTy'rAC
Cuts at: 638 1448 638
Size: 810 3599
HindIII A'AGCT_T
Cuts at: 648 648
Size: 4409
KasI G'GCGC_C
Cuts at: 1839 2087 1839
Size: 248 4161
KpnI G_GTAC'C
Cuts at: 1429 1429
Size: 4409
MfeI C'AATT_G
Cuts at: 641 641
Size: 4409
MluI A'CGCG_T
Cuts at: 3842 3842
Size: 4409
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 110 3080 3264 110
Size: 2970 184 1255
Fragments arranged by size:
2970 1255 184
MscI TGG'CCA
Cuts at: 2170 2170
Size: 4409
MslI CAynn'nnrTG
Cuts at: 2398 2525 2398
Size: 127 4282
MspA1I CmG'CkG
Cuts at: 463 1520 1834 2194 3207 3452 463
Size: 1057 314 360 1013 245 1420
Fragments arranged by size:
1420 1057 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2590 2590
Size: 4409
NarI GG'CG_CC
Cuts at: 1840 2088 1840
Size: 248 4161
NgoMIV G'CCGG_C
Cuts at: 2588 2588
Size: 4409
NspI r_CATG'y
Cuts at: 1462 1962 2869 1462
Size: 500 907 3002
Fragments arranged by size:
3002 907 500
PasI CC'CwG_GG
Cuts at: 573 573
Size: 4409
PciI A'CATG_T
Cuts at: 2865 2865
Size: 4409
PflMI CCAn_nnn'nTGG
Cuts at: 744 744
Size: 4409
PmeI GTTT'AAAC
Cuts at: 1408 1408
Size: 4409
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 972 1004 3548 3580 972
Size: 32 2544 32 1801
Fragments arranged by size:
2544 1801 32 32
PstI C_TGCA'G
Cuts at: 1456 2141 1456
Size: 685 3724
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4409
PvuII CAG'CTG
Cuts at: 1834 2194 1834
Size: 360 4049
RsrII CG'GwC_CG
Cuts at: 2604 2604
Size: 4409
SacI G_AGCT'C
Cuts at: 328 1423 328
Size: 1095 3314
SalI G'TCGA_C
Cuts at: 636 1446 636
Size: 810 3599
SapI GCTCTTCn'nnn_
Cuts at: 316 2432 2642 316
Size: 2116 210 2083
Fragments arranged by size:
2116 2083 210
SbfI CC_TGCA'GG
Cuts at: 1456 1456
Size: 4409
ScaI AGT'ACT
Cuts at: 3926 3926
Size: 4409
SfcI C'TryA_G
Cuts at: 33 785 1452 2137 3130 3321 33
Size: 752 667 685 993 191 1121
Fragments arranged by size:
1121 993 752 685 667 191
SfoI GGC'GCC
Cuts at: 1841 2089 1841
Size: 248 4161
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4409
SmaI CCC'GGG
Cuts at: 727 1431 727
Size: 704 3705
SmlI C'TyrA_G
Cuts at: 1005 1564 2479 2971 3233 1005
Size: 559 915 492 262 2181
Fragments arranged by size:
2181 915 559 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4409
SphI G_CATG'C
Cuts at: 1462 1962 1462
Size: 500 3909
SspI AAT'ATT
Cuts at: 217 217
Size: 4409
StyI C'CwwG_G
Cuts at: 1235 1543 1897 1235
Size: 308 354 3747
Fragments arranged by size:
3747 354 308
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 167 1992 2125 2584 2793 167
Size: 1825 133 459 209 1783
Fragments arranged by size:
1825 1783 459 209 133
TatI w'GTAC_w
Cuts at: 519 3924 519
Size: 3405 1004
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3926 3958 3926
Size: 32 4377
Tth111I GACn'n_nGTC
Cuts at: 2206 2206
Size: 4409
XbaI T'CTAG_A
Cuts at: 1440 1440
Size: 4409
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 451 1904 451
Size: 1453 2956
XmaI C'CCGG_G
Cuts at: 725 1429 725
Size: 704 3705
XmnI GAAnn'nnTTC
Cuts at: 287 491 987 287
Size: 204 496 3709
Fragments arranged by size:
3709 496 204
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgZI BtsI ClaI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PasI PciI PflMI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI BpmI
BseRI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I BtgI CspCI DraIII EcoNI EcoRV FseI
FspAI HpaI NcoI NdeI NheI NotI NruI NsiI
PacI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI ZraI