Restriction map for
pF1KE0283
(Circular) (Six-base) MAPSORT of: pF1KE0283.seq Check: 7232 from: 1 to: 4082
pF1KE0283 4082 bp
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AccI GT'mk_AC
Cuts at: 1120 1120
Size: 4082
Acc65I G'GTAC_C
Cuts at: 500 1098 500
Size: 598 3484
AclI AA'CG_TT
Cuts at: 3922 3922
Size: 4082
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 682 1907 2339 3086 3354 682
Size: 1225 432 747 268 1410
Fragments arranged by size:
1410 1225 747 432 268
AflIII A'CryG_T
Cuts at: 680 826 1434 2538 3515 680
Size: 146 608 1104 977 1247
Fragments arranged by size:
1247 1104 977 608 146
AgeI A'CCGG_T
Cuts at: 2470 2470
Size: 4082
AhdI GACnn_n'nnGTC
Cuts at: 366 366
Size: 4082
AleI CACnn'nnGTG
Cuts at: 883 883
Size: 4082
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1017 1049 1017
Size: 32 4050
AlwNI CAG_nnn'CTG
Cuts at: 1387 2954 1387
Size: 1567 2515
ApaLI G'TGCA_C
Cuts at: 2852 2852
Size: 4082
ApoI r'AATT_y
Cuts at: 439 1086 3435 3691 439
Size: 647 2349 256 830
Fragments arranged by size:
2349 830 647 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4082
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4082
AvaI C'yCGr_G
Cuts at: 181 620 1102 181
Size: 439 482 3161
Fragments arranged by size:
3161 482 439
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 318 351 318
Size: 33 4049
BamHI G'GATC_C
Cuts at: 1107 1107
Size: 4082
BanI G'GyrC_C
Cuts at: 500 1098 1512 1760 1795 500
Size: 598 414 248 35 2787
Fragments arranged by size:
2787 598 414 248 35
BanII G_rGCy'C
Cuts at: 1096 2126 1096
Size: 1030 3052
BbeI G_GCGC'C
Cuts at: 1516 1764 1516
Size: 248 3834
BbsI GAAGACnn'nnnn_
Cuts at: 115 195 115
Size: 80 4002
BbvCI CC'TCA_GC
Cuts at: 800 800
Size: 4082
BciVI GTATCCnnnnn_n'
Cuts at: 312 1980 2741 4082 312
Size: 1668 761 1341 312
Fragments arranged by size:
1668 1341 761 312
BclI T'GATC_A
Cuts at: 1064 1602 1064
Size: 538 3544
BglII A'GATC_T
Cuts at: 1597 3634 1597
Size: 2037 2045
BlpI GC'TnA_GC
Cuts at: 298 1194 298
Size: 896 3186
Bme1580I G_kGCm'C
Cuts at: 1707 1800 2856 1707
Size: 93 1056 2933
Fragments arranged by size:
2933 1056 93
BmgBI CAC'GTC
Cuts at: 683 683
Size: 4082
BmrI ACTGGGnnnn_n'
Cuts at: 1294 1375 1710 3854 1294
Size: 81 335 2144 1522
Fragments arranged by size:
2144 1522 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2449 2449
Size: 4082
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 112 340 893 112
Size: 228 553 3301
Fragments arranged by size:
3301 553 228
Bpu10I CC'TnA_GC
Cuts at: 800 800
Size: 4082
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1002 1258 2137 2629 2927 1002
Size: 256 879 492 298 2157
Fragments arranged by size:
2157 879 492 298 256
BsaI GGTCTCn'nnnn_
Cuts at: 460 688 3786 460
Size: 228 3098 756
Fragments arranged by size:
3098 756 228
BsaAI yAC'GTr
Cuts at: 827 1435 827
Size: 608 3474
BsaBI GATnn'nnATC
Cuts at: 6 1596 3633 6
Size: 1590 2037 455
Fragments arranged by size:
2037 1590 455
BsaHI Gr'CG_yC
Cuts at: 1513 1761 2463 3959 1513
Size: 248 702 1496 1636
Fragments arranged by size:
1636 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1271 1792 2470 2744 2891 3302 1271
Size: 521 678 274 147 411 2051
Fragments arranged by size:
2051 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 250 280 1017 1047 250
Size: 30 737 30 3285
Fragments arranged by size:
3285 737 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 499 499
Size: 4082
BseYI C'CCAG_C
Cuts at: 450 651 1507 2265 2842 3338 450
Size: 201 856 758 577 496 1194
Fragments arranged by size:
1194 856 758 577 496 201
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 422 422
Size: 4082
BsiEI CG_ry'CG
Cuts at: 104 1624 1670 2878 104
Size: 1520 46 1208 1308
Fragments arranged by size:
1520 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1096 1874 2064 2856 1096
Size: 778 190 792 2322
Fragments arranged by size:
2322 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1096 1707 1800 1874 2064 2126 2856 1096
Size: 611 93 74 190 62 730 2322
Fragments arranged by size:
2322 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3302 3302
Size: 4082
BspHI T'CATG_A
Cuts at: 242 512 3258 242
Size: 270 2746 1066
Fragments arranged by size:
2746 1066 270
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1132 1346 1648 2029 1132
Size: 214 302 381 3185
Fragments arranged by size:
3185 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2374 2428 3912 4077 50
Size: 2324 54 1484 165 55
Fragments arranged by size:
2324 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1994 1994
Size: 4082
BsrFI r'CCGG_y
Cuts at: 806 2080 2261 2470 806
Size: 1274 181 209 2418
Fragments arranged by size:
2418 1274 209 181
BssSI C'ACGA_G
Cuts at: 2353 2711 2353
Size: 358 3724
BstAPI GCAn_nnn'nTGC
Cuts at: 527 1459 527
Size: 932 3150
BstBI TT'CG_AA
Cuts at: 2443 2443
Size: 4082
BstXI CCAn_nnnn'nTGG
Cuts at: 114 216 114
Size: 102 3980
BstYI r'GATC_y
Cuts at: 1 616 647 1107 1574 1597 1932 2178 3190
Size: 615 31 460 467 23 335 246 1012
Cuts at: 3190 3276 3288 3634 1
Size: 86 12 346 449
Fragments arranged by size:
1012 615 467 460 449 346 335 246
86 31 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4082
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2032 2213 2376 2032
Size: 181 163 3738
Fragments arranged by size:
3738 181 163
BtsI GCAGTG_nn'
Cuts at: 1325 1325
Size: 4082
DraI TTT'AAA
Cuts at: 1081 1081
Size: 4082
DrdI GACnn_nn'nnGTC
Cuts at: 1788 2646 1788
Size: 858 3224
EaeI y'GGCC_r
Cuts at: 1667 1841 2232 2259 3604 1667
Size: 174 391 27 1345 2145
Fragments arranged by size:
2145 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1667 1667
Size: 4082
EarI CTCTTCn'nnn_
Cuts at: 2105 2315 2105
Size: 210 3872
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2290 2600 2746 3892 2290
Size: 310 146 1146 2480
Fragments arranged by size:
2480 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1094 1094
Size: 4082
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 112 340 682 893 1907 2339 3086 3354 112
Size: 228 342 211 1014 432 747 268 840
Fragments arranged by size:
1014 840 747 432 342 268 228 211
EcoNI CCTnn'n_nnAGG
Cuts at: 370 370
Size: 4082
EcoO109I rG'GnC_Cy
Cuts at: 360 1221 360
Size: 861 3221
EcoRI G'AATT_C
Cuts at: 439 1086 439
Size: 647 3435
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2101 2101
Size: 4082
FspI TGC'GCA
Cuts at: 1863 1863
Size: 4082
HaeII r_GCGC'y
Cuts at: 1516 1764 2786 3765 1516
Size: 248 1022 979 1833
Fragments arranged by size:
1833 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 352 384 1924 1954 1956 1986 352
Size: 32 1540 30 2 30 2448
Fragments arranged by size:
2448 1540 32 30 30 2
HincII GTy'rAC
Cuts at: 1121 1121
Size: 4082
KasI G'GCGC_C
Cuts at: 1512 1760 1512
Size: 248 3834
KpnI G_GTAC'C
Cuts at: 504 1102 504
Size: 598 3484
MluI A'CGCG_T
Cuts at: 3515 3515
Size: 4082
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2753 2937 2753
Size: 184 3898
MscI TGG'CCA
Cuts at: 1843 1843
Size: 4082
MslI CAynn'nnrTG
Cuts at: 112 214 259 283 751 844 883 955 1051
Size: 102 45 24 468 93 39 72 96
Cuts at: 1051 2071 2198 112
Size: 1020 127 1996
Fragments arranged by size:
1996 1020 468 127 102 96 93 72
45 39 24
MspA1I CmG'CkG
Cuts at: 1193 1507 1867 2880 3125 1193
Size: 314 360 1013 245 2150
Fragments arranged by size:
2150 1013 360 314 245
NaeI GCC'GGC
Cuts at: 808 2263 808
Size: 1455 2627
NarI GG'CG_CC
Cuts at: 1513 1761 1513
Size: 248 3834
NcoI C'CATG_G
Cuts at: 107 107
Size: 4082
NgoMIV G'CCGG_C
Cuts at: 806 2261 806
Size: 1455 2627
NspI r_CATG'y
Cuts at: 1135 1635 2542 1135
Size: 500 907 2675
Fragments arranged by size:
2675 907 500
PciI A'CATG_T
Cuts at: 2538 2538
Size: 4082
PflMI CCAn_nnn'nTGG
Cuts at: 843 843
Size: 4082
PfoI T'CCnGG_A
Cuts at: 356 356
Size: 4082
PmeI GTTT'AAAC
Cuts at: 1081 1081
Size: 4082
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1017 1049 3221 3253 1017
Size: 32 2172 32 1846
Fragments arranged by size:
2172 1846 32 32
PpuMI rG'GwC_Cy
Cuts at: 360 360
Size: 4082
PshAI GACnn'nnGTC
Cuts at: 589 589
Size: 4082
PstI C_TGCA'G
Cuts at: 1005 1129 1814 1005
Size: 124 685 3273
Fragments arranged by size:
3273 685 124
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4082
PvuII CAG'CTG
Cuts at: 1507 1867 1507
Size: 360 3722
RsrII CG'GwC_CG
Cuts at: 2277 2277
Size: 4082
SacI G_AGCT'C
Cuts at: 1096 1096
Size: 4082
SalI G'TCGA_C
Cuts at: 1119 1119
Size: 4082
SapI GCTCTTCn'nnn_
Cuts at: 2105 2315 2105
Size: 210 3872
SbfI CC_TGCA'GG
Cuts at: 1129 1129
Size: 4082
ScaI AGT'ACT
Cuts at: 1019 3599 1019
Size: 2580 1502
SfcI C'TryA_G
Cuts at: 33 612 975 1001 1125 1810 2803 2994 33
Size: 579 363 26 124 685 993 191 1121
Fragments arranged by size:
1121 993 685 579 363 191 124 26
SfoI GGC'GCC
Cuts at: 1514 1762 1514
Size: 248 3834
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4082
SmaI CCC'GGG
Cuts at: 183 1104 183
Size: 921 3161
SmlI C'TyrA_G
Cuts at: 981 1237 2152 2644 2906 981
Size: 256 915 492 262 2157
Fragments arranged by size:
2157 915 492 262 256
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4082
SphI G_CATG'C
Cuts at: 1135 1635 1135
Size: 500 3582
StuI AGG'CCT
Cuts at: 158 158
Size: 4082
StyI C'CwwG_G
Cuts at: 107 371 566 1216 1570 107
Size: 264 195 650 354 2619
Fragments arranged by size:
2619 650 354 264 195
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 181 297 1665 1798 2257 2466 181
Size: 116 1368 133 459 209 1797
Fragments arranged by size:
1797 1368 459 209 133 116
TatI w'GTAC_w
Cuts at: 287 1017 3597 287
Size: 730 2580 772
Fragments arranged by size:
2580 772 730
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 250 282 3599 3631 250
Size: 32 3317 32 701
Fragments arranged by size:
3317 701 32 32
Tth111I GACn'n_nGTC
Cuts at: 1879 1879
Size: 4082
XbaI T'CTAG_A
Cuts at: 1113 1113
Size: 4082
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 216 390 1577 216
Size: 174 1187 2721
Fragments arranged by size:
2721 1187 174
XmaI C'CCGG_G
Cuts at: 181 1102 181
Size: 921 3161
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AlfI ApaI AscI AvrII
BcgI BfrBI BglI BmtI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PmlI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI XmnI ZraI