(Circular) (Six-base) MAPSORT of: pF1KE0283.seq Check: 7232 from: 1 to: 4082 pF1KE0283 4082 bp With 182 enzymes: SgfI * April 24, 2009 13:12 .. AccI GT'mk_AC Cuts at: 1120 1120 Size: 4082 Acc65I G'GTAC_C Cuts at: 500 1098 500 Size: 598 3484 AclI AA'CG_TT Cuts at: 3922 3922 Size: 4082 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 682 1907 2339 3086 3354 682 Size: 1225 432 747 268 1410 Fragments arranged by size: 1410 1225 747 432 268 AflIII A'CryG_T Cuts at: 680 826 1434 2538 3515 680 Size: 146 608 1104 977 1247 Fragments arranged by size: 1247 1104 977 608 146 AgeI A'CCGG_T Cuts at: 2470 2470 Size: 4082 AhdI GACnn_n'nnGTC Cuts at: 366 366 Size: 4082 AleI CACnn'nnGTG Cuts at: 883 883 Size: 4082 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1017 1049 1017 Size: 32 4050 AlwNI CAG_nnn'CTG Cuts at: 1387 2954 1387 Size: 1567 2515 ApaLI G'TGCA_C Cuts at: 2852 2852 Size: 4082 ApoI r'AATT_y Cuts at: 439 1086 3435 3691 439 Size: 647 2349 256 830 Fragments arranged by size: 2349 830 647 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4082 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4082 AvaI C'yCGr_G Cuts at: 181 620 1102 181 Size: 439 482 3161 Fragments arranged by size: 3161 482 439 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 318 351 318 Size: 33 4049 BamHI G'GATC_C Cuts at: 1107 1107 Size: 4082 BanI G'GyrC_C Cuts at: 500 1098 1512 1760 1795 500 Size: 598 414 248 35 2787 Fragments arranged by size: 2787 598 414 248 35 BanII G_rGCy'C Cuts at: 1096 2126 1096 Size: 1030 3052 BbeI G_GCGC'C Cuts at: 1516 1764 1516 Size: 248 3834 BbsI GAAGACnn'nnnn_ Cuts at: 115 195 115 Size: 80 4002 BbvCI CC'TCA_GC Cuts at: 800 800 Size: 4082 BciVI GTATCCnnnnn_n' Cuts at: 312 1980 2741 4082 312 Size: 1668 761 1341 312 Fragments arranged by size: 1668 1341 761 312 BclI T'GATC_A Cuts at: 1064 1602 1064 Size: 538 3544 BglII A'GATC_T Cuts at: 1597 3634 1597 Size: 2037 2045 BlpI GC'TnA_GC Cuts at: 298 1194 298 Size: 896 3186 Bme1580I G_kGCm'C Cuts at: 1707 1800 2856 1707 Size: 93 1056 2933 Fragments arranged by size: 2933 1056 93 BmgBI CAC'GTC Cuts at: 683 683 Size: 4082 BmrI ACTGGGnnnn_n' Cuts at: 1294 1375 1710 3854 1294 Size: 81 335 2144 1522 Fragments arranged by size: 2144 1522 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2449 2449 Size: 4082 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 112 340 893 112 Size: 228 553 3301 Fragments arranged by size: 3301 553 228 Bpu10I CC'TnA_GC Cuts at: 800 800 Size: 4082 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1002 1258 2137 2629 2927 1002 Size: 256 879 492 298 2157 Fragments arranged by size: 2157 879 492 298 256 BsaI GGTCTCn'nnnn_ Cuts at: 460 688 3786 460 Size: 228 3098 756 Fragments arranged by size: 3098 756 228 BsaAI yAC'GTr Cuts at: 827 1435 827 Size: 608 3474 BsaBI GATnn'nnATC Cuts at: 6 1596 3633 6 Size: 1590 2037 455 Fragments arranged by size: 2037 1590 455 BsaHI Gr'CG_yC Cuts at: 1513 1761 2463 3959 1513 Size: 248 702 1496 1636 Fragments arranged by size: 1636 1496 702 248 BsaWI w'CCGG_w Cuts at: 1271 1792 2470 2744 2891 3302 1271 Size: 521 678 274 147 411 2051 Fragments arranged by size: 2051 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 250 280 1017 1047 250 Size: 30 737 30 3285 Fragments arranged by size: 3285 737 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 499 499 Size: 4082 BseYI C'CCAG_C Cuts at: 450 651 1507 2265 2842 3338 450 Size: 201 856 758 577 496 1194 Fragments arranged by size: 1194 856 758 577 496 201 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 422 422 Size: 4082 BsiEI CG_ry'CG Cuts at: 104 1624 1670 2878 104 Size: 1520 46 1208 1308 Fragments arranged by size: 1520 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 1096 1874 2064 2856 1096 Size: 778 190 792 2322 Fragments arranged by size: 2322 792 778 190 Bsp1286I G_dGCh'C Cuts at: 1096 1707 1800 1874 2064 2126 2856 1096 Size: 611 93 74 190 62 730 2322 Fragments arranged by size: 2322 730 611 190 93 74 62 BspEI T'CCGG_A Cuts at: 3302 3302 Size: 4082 BspHI T'CATG_A Cuts at: 242 512 3258 242 Size: 270 2746 1066 Fragments arranged by size: 2746 1066 270 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1132 1346 1648 2029 1132 Size: 214 302 381 3185 Fragments arranged by size: 3185 381 302 214 BsrBI CCG'CTC Cuts at: 50 2374 2428 3912 4077 50 Size: 2324 54 1484 165 55 Fragments arranged by size: 2324 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1994 1994 Size: 4082 BsrFI r'CCGG_y Cuts at: 806 2080 2261 2470 806 Size: 1274 181 209 2418 Fragments arranged by size: 2418 1274 209 181 BssSI C'ACGA_G Cuts at: 2353 2711 2353 Size: 358 3724 BstAPI GCAn_nnn'nTGC Cuts at: 527 1459 527 Size: 932 3150 BstBI TT'CG_AA Cuts at: 2443 2443 Size: 4082 BstXI CCAn_nnnn'nTGG Cuts at: 114 216 114 Size: 102 3980 BstYI r'GATC_y Cuts at: 1 616 647 1107 1574 1597 1932 2178 3190 Size: 615 31 460 467 23 335 246 1012 Cuts at: 3190 3276 3288 3634 1 Size: 86 12 346 449 Fragments arranged by size: 1012 615 467 460 449 346 335 246 86 31 23 12 BtgI C'CryG_G Cuts at: 107 107 Size: 4082 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2032 2213 2376 2032 Size: 181 163 3738 Fragments arranged by size: 3738 181 163 BtsI GCAGTG_nn' Cuts at: 1325 1325 Size: 4082 DraI TTT'AAA Cuts at: 1081 1081 Size: 4082 DrdI GACnn_nn'nnGTC Cuts at: 1788 2646 1788 Size: 858 3224 EaeI y'GGCC_r Cuts at: 1667 1841 2232 2259 3604 1667 Size: 174 391 27 1345 2145 Fragments arranged by size: 2145 1345 391 174 27 EagI C'GGCC_G Cuts at: 1667 1667 Size: 4082 EarI CTCTTCn'nnn_ Cuts at: 2105 2315 2105 Size: 210 3872 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2290 2600 2746 3892 2290 Size: 310 146 1146 2480 Fragments arranged by size: 2480 1146 310 146 EcoICRI GAG'CTC Cuts at: 1094 1094 Size: 4082 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 112 340 682 893 1907 2339 3086 3354 112 Size: 228 342 211 1014 432 747 268 840 Fragments arranged by size: 1014 840 747 432 342 268 228 211 EcoNI CCTnn'n_nnAGG Cuts at: 370 370 Size: 4082 EcoO109I rG'GnC_Cy Cuts at: 360 1221 360 Size: 861 3221 EcoRI G'AATT_C Cuts at: 439 1086 439 Size: 647 3435 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2101 2101 Size: 4082 FspI TGC'GCA Cuts at: 1863 1863 Size: 4082 HaeII r_GCGC'y Cuts at: 1516 1764 2786 3765 1516 Size: 248 1022 979 1833 Fragments arranged by size: 1833 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 352 384 1924 1954 1956 1986 352 Size: 32 1540 30 2 30 2448 Fragments arranged by size: 2448 1540 32 30 30 2 HincII GTy'rAC Cuts at: 1121 1121 Size: 4082 KasI G'GCGC_C Cuts at: 1512 1760 1512 Size: 248 3834 KpnI G_GTAC'C Cuts at: 504 1102 504 Size: 598 3484 MluI A'CGCG_T Cuts at: 3515 3515 Size: 4082 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2753 2937 2753 Size: 184 3898 MscI TGG'CCA Cuts at: 1843 1843 Size: 4082 MslI CAynn'nnrTG Cuts at: 112 214 259 283 751 844 883 955 1051 Size: 102 45 24 468 93 39 72 96 Cuts at: 1051 2071 2198 112 Size: 1020 127 1996 Fragments arranged by size: 1996 1020 468 127 102 96 93 72 45 39 24 MspA1I CmG'CkG Cuts at: 1193 1507 1867 2880 3125 1193 Size: 314 360 1013 245 2150 Fragments arranged by size: 2150 1013 360 314 245 NaeI GCC'GGC Cuts at: 808 2263 808 Size: 1455 2627 NarI GG'CG_CC Cuts at: 1513 1761 1513 Size: 248 3834 NcoI C'CATG_G Cuts at: 107 107 Size: 4082 NgoMIV G'CCGG_C Cuts at: 806 2261 806 Size: 1455 2627 NspI r_CATG'y Cuts at: 1135 1635 2542 1135 Size: 500 907 2675 Fragments arranged by size: 2675 907 500 PciI A'CATG_T Cuts at: 2538 2538 Size: 4082 PflMI CCAn_nnn'nTGG Cuts at: 843 843 Size: 4082 PfoI T'CCnGG_A Cuts at: 356 356 Size: 4082 PmeI GTTT'AAAC Cuts at: 1081 1081 Size: 4082 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1017 1049 3221 3253 1017 Size: 32 2172 32 1846 Fragments arranged by size: 2172 1846 32 32 PpuMI rG'GwC_Cy Cuts at: 360 360 Size: 4082 PshAI GACnn'nnGTC Cuts at: 589 589 Size: 4082 PstI C_TGCA'G Cuts at: 1005 1129 1814 1005 Size: 124 685 3273 Fragments arranged by size: 3273 685 124 PvuI CG_AT'CG Cuts at: 104 104 Size: 4082 PvuII CAG'CTG Cuts at: 1507 1867 1507 Size: 360 3722 RsrII CG'GwC_CG Cuts at: 2277 2277 Size: 4082 SacI G_AGCT'C Cuts at: 1096 1096 Size: 4082 SalI G'TCGA_C Cuts at: 1119 1119 Size: 4082 SapI GCTCTTCn'nnn_ Cuts at: 2105 2315 2105 Size: 210 3872 SbfI CC_TGCA'GG Cuts at: 1129 1129 Size: 4082 ScaI AGT'ACT Cuts at: 1019 3599 1019 Size: 2580 1502 SfcI C'TryA_G Cuts at: 33 612 975 1001 1125 1810 2803 2994 33 Size: 579 363 26 124 685 993 191 1121 Fragments arranged by size: 1121 993 685 579 363 191 124 26 SfoI GGC'GCC Cuts at: 1514 1762 1514 Size: 248 3834 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4082 SmaI CCC'GGG Cuts at: 183 1104 183 Size: 921 3161 SmlI C'TyrA_G Cuts at: 981 1237 2152 2644 2906 981 Size: 256 915 492 262 2157 Fragments arranged by size: 2157 915 492 262 256 SpeI A'CTAG_T Cuts at: 67 67 Size: 4082 SphI G_CATG'C Cuts at: 1135 1635 1135 Size: 500 3582 StuI AGG'CCT Cuts at: 158 158 Size: 4082 StyI C'CwwG_G Cuts at: 107 371 566 1216 1570 107 Size: 264 195 650 354 2619 Fragments arranged by size: 2619 650 354 264 195 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 181 297 1665 1798 2257 2466 181 Size: 116 1368 133 459 209 1797 Fragments arranged by size: 1797 1368 459 209 133 116 TatI w'GTAC_w Cuts at: 287 1017 3597 287 Size: 730 2580 772 Fragments arranged by size: 2580 772 730 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 250 282 3599 3631 250 Size: 32 3317 32 701 Fragments arranged by size: 3317 701 32 32 Tth111I GACn'n_nGTC Cuts at: 1879 1879 Size: 4082 XbaI T'CTAG_A Cuts at: 1113 1113 Size: 4082 XcmI CCAnnnn_n'nnnnTGG Cuts at: 216 390 1577 216 Size: 174 1187 2721 Fragments arranged by size: 2721 1187 174 XmaI C'CCGG_G Cuts at: 181 1102 181 Size: 921 3161 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AlfI ApaI AscI AvrII BcgI BfrBI BglI BmtI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PasI PmlI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI