(Circular) (Six-base) MAPSORT of: pF1KE0284.seq Check: 8904 from: 1 to: 4124 pF1KE0284 4124 bp With 182 enzymes: SgfI * April 24, 2009 13:12 .. AccI GT'mk_AC Cuts at: 652 1162 652 Size: 510 3614 Acc65I G'GTAC_C Cuts at: 1140 1140 Size: 4124 AclI AA'CG_TT Cuts at: 3964 3964 Size: 4124 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 257 1949 2381 3128 3396 257 Size: 1692 432 747 268 985 Fragments arranged by size: 1692 985 747 432 268 AflIII A'CryG_T Cuts at: 662 1476 2580 3557 662 Size: 814 1104 977 1229 Fragments arranged by size: 1229 1104 977 814 AgeI A'CCGG_T Cuts at: 2512 2512 Size: 4124 AlwNI CAG_nnn'CTG Cuts at: 1429 2996 1429 Size: 1567 2557 ApaI G_GGCC'C Cuts at: 220 220 Size: 4124 ApaLI G'TGCA_C Cuts at: 2894 2894 Size: 4124 ApoI r'AATT_y Cuts at: 1128 3477 3733 1128 Size: 2349 256 1519 Fragments arranged by size: 2349 1519 256 AseI AT'TA_AT Cuts at: 20 926 20 Size: 906 3218 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4124 AvaI C'yCGr_G Cuts at: 219 238 1144 219 Size: 19 906 3199 Fragments arranged by size: 3199 906 19 BamHI G'GATC_C Cuts at: 1149 1149 Size: 4124 BanI G'GyrC_C Cuts at: 1140 1554 1802 1837 1140 Size: 414 248 35 3427 Fragments arranged by size: 3427 414 248 35 BanII G_rGCy'C Cuts at: 220 418 716 1138 2168 220 Size: 198 298 422 1030 2176 Fragments arranged by size: 2176 1030 422 298 198 BbeI G_GCGC'C Cuts at: 1558 1806 1558 Size: 248 3876 BbsI GAAGACnn'nnnn_ Cuts at: 1075 1075 Size: 4124 BbvCI CC'TCA_GC Cuts at: 1000 1000 Size: 4124 BciVI GTATCCnnnnn_n' Cuts at: 2022 2783 4124 2022 Size: 761 1341 2022 Fragments arranged by size: 2022 1341 761 BclI T'GATC_A Cuts at: 1644 1644 Size: 4124 BglII A'GATC_T Cuts at: 1639 3676 1639 Size: 2037 2087 BlpI GC'TnA_GC Cuts at: 1236 1236 Size: 4124 Bme1580I G_kGCm'C Cuts at: 220 1749 1842 2898 220 Size: 1529 93 1056 1446 Fragments arranged by size: 1529 1446 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 858 1336 1417 1752 3896 858 Size: 478 81 335 2144 1086 Fragments arranged by size: 2144 1086 478 335 81 BmtI G_CTAG'C Cuts at: 349 349 Size: 4124 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 471 2491 471 Size: 2020 2104 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 383 790 383 Size: 407 3717 Bpu10I CC'TnA_GC Cuts at: 1000 1000 Size: 4124 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1300 2179 2671 2969 1300 Size: 879 492 298 2455 Fragments arranged by size: 2455 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 174 3828 174 Size: 3654 470 BsaAI yAC'GTr Cuts at: 569 1477 569 Size: 908 3216 BsaBI GATnn'nnATC Cuts at: 6 1638 3675 6 Size: 1632 2037 455 Fragments arranged by size: 2037 1632 455 BsaHI Gr'CG_yC Cuts at: 1555 1803 2505 4001 1555 Size: 248 702 1496 1678 Fragments arranged by size: 1678 1496 702 248 BsaWI w'CCGG_w Cuts at: 444 1313 1834 2512 2786 2933 3344 444 Size: 869 521 678 274 147 411 1224 Fragments arranged by size: 1224 869 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 105 135 152 182 634 664 105 Size: 30 17 30 452 30 3565 Fragments arranged by size: 3565 452 30 30 30 17 BseYI C'CCAG_C Cuts at: 282 411 1549 2307 2884 3380 282 Size: 129 1138 758 577 496 1026 Fragments arranged by size: 1138 1026 758 577 496 129 BsiEI CG_ry'CG Cuts at: 104 1666 1712 2920 104 Size: 1562 46 1208 1308 Fragments arranged by size: 1562 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 716 1138 1916 2106 2898 716 Size: 422 778 190 792 1942 Fragments arranged by size: 1942 792 778 422 190 Bsp1286I G_dGCh'C Cuts at: 220 418 716 1138 1749 1842 1916 2106 2168 Size: 198 298 422 611 93 74 190 62 Cuts at: 2168 2898 220 Size: 730 1446 Fragments arranged by size: 1446 730 611 422 298 198 190 93 74 62 BspEI T'CCGG_A Cuts at: 444 3344 444 Size: 2900 1224 BspHI T'CATG_A Cuts at: 1064 3300 1064 Size: 2236 1888 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1174 1388 1690 2071 1174 Size: 214 302 381 3227 Fragments arranged by size: 3227 381 302 214 BsrBI CCG'CTC Cuts at: 50 2416 2470 3954 4119 50 Size: 2366 54 1484 165 55 Fragments arranged by size: 2366 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 515 1052 2036 515 Size: 537 984 2603 Fragments arranged by size: 2603 984 537 BsrFI r'CCGG_y Cuts at: 289 1019 2122 2303 2512 289 Size: 730 1103 181 209 1901 Fragments arranged by size: 1901 1103 730 209 181 BsrGI T'GTAC_A Cuts at: 706 706 Size: 4124 BssSI C'ACGA_G Cuts at: 2395 2753 2395 Size: 358 3766 BstAPI GCAn_nnn'nTGC Cuts at: 765 1501 765 Size: 736 3388 BstBI TT'CG_AA Cuts at: 305 2485 305 Size: 2180 1944 BstXI CCAn_nnnn'nTGG Cuts at: 474 490 474 Size: 16 4108 BstYI r'GATC_y Cuts at: 1 810 1149 1616 1639 1974 2220 3232 3318 Size: 809 339 467 23 335 246 1012 86 Cuts at: 3318 3330 3676 1 Size: 12 346 449 Fragments arranged by size: 1012 809 467 449 346 339 335 246 86 23 12 Bsu36I CC'TnA_GG Cuts at: 593 593 Size: 4124 BtgI C'CryG_G Cuts at: 107 483 500 638 734 953 107 Size: 376 17 138 96 219 3278 Fragments arranged by size: 3278 376 219 138 96 17 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2074 2255 2418 2074 Size: 181 163 3780 Fragments arranged by size: 3780 181 163 BtsI GCAGTG_nn' Cuts at: 925 1367 925 Size: 442 3682 DraI TTT'AAA Cuts at: 1123 1123 Size: 4124 DrdI GACnn_nn'nnGTC Cuts at: 859 1830 2688 859 Size: 971 858 2295 Fragments arranged by size: 2295 971 858 EaeI y'GGCC_r Cuts at: 630 1709 1883 2274 2301 3646 630 Size: 1079 174 391 27 1345 1108 Fragments arranged by size: 1345 1108 1079 391 174 27 EagI C'GGCC_G Cuts at: 1709 1709 Size: 4124 EarI CTCTTCn'nnn_ Cuts at: 2147 2357 2147 Size: 210 3914 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 919 2332 2642 2788 3934 919 Size: 1413 310 146 1146 1109 Fragments arranged by size: 1413 1146 1109 310 146 EcoICRI GAG'CTC Cuts at: 714 1136 714 Size: 422 3702 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 257 383 790 1949 2381 3128 3396 257 Size: 126 407 1159 432 747 268 985 Fragments arranged by size: 1159 985 747 432 407 268 126 EcoO109I rG'GnC_Cy Cuts at: 996 1263 996 Size: 267 3857 EcoRI G'AATT_C Cuts at: 1128 1128 Size: 4124 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2143 2143 Size: 4124 FseI GG_CCGG'CC Cuts at: 1023 1023 Size: 4124 FspI TGC'GCA Cuts at: 1905 1905 Size: 4124 HaeII r_GCGC'y Cuts at: 1558 1806 2828 3807 1558 Size: 248 1022 979 1875 Fragments arranged by size: 1875 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 105 137 152 184 576 608 634 666 1076 Size: 32 15 32 392 32 26 32 410 Cuts at: 1076 1108 1966 1996 1998 2028 105 Size: 32 858 30 2 30 2201 Fragments arranged by size: 2201 858 410 392 32 32 32 32 32 30 30 26 15 2 HincII GTy'rAC Cuts at: 1163 1163 Size: 4124 KasI G'GCGC_C Cuts at: 1554 1802 1554 Size: 248 3876 KpnI G_GTAC'C Cuts at: 1144 1144 Size: 4124 MluI A'CGCG_T Cuts at: 3557 3557 Size: 4124 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2795 2979 2795 Size: 184 3940 MscI TGG'CCA Cuts at: 1885 1885 Size: 4124 MslI CAynn'nnrTG Cuts at: 665 2113 2240 665 Size: 1448 127 2549 Fragments arranged by size: 2549 1448 127 MspA1I CmG'CkG Cuts at: 127 286 1031 1235 1549 1909 2922 3167 127 Size: 159 745 204 314 360 1013 245 1084 Fragments arranged by size: 1084 1013 745 360 314 245 204 159 NaeI GCC'GGC Cuts at: 1021 2305 1021 Size: 1284 2840 NarI GG'CG_CC Cuts at: 1555 1803 1555 Size: 248 3876 NcoI C'CATG_G Cuts at: 107 500 638 734 953 107 Size: 393 138 96 219 3278 Fragments arranged by size: 3278 393 219 138 96 NgoMIV G'CCGG_C Cuts at: 1019 2303 1019 Size: 1284 2840 NheI G'CTAG_C Cuts at: 345 345 Size: 4124 NspI r_CATG'y Cuts at: 666 1177 1677 2584 666 Size: 511 500 907 2206 Fragments arranged by size: 2206 907 511 500 PasI CC'CwG_GG Cuts at: 152 152 Size: 4124 PciI A'CATG_T Cuts at: 662 2580 662 Size: 1918 2206 PflMI CCAn_nnn'nTGG Cuts at: 888 888 Size: 4124 PmeI GTTT'AAAC Cuts at: 1123 1123 Size: 4124 PmlI CAC'GTG Cuts at: 569 569 Size: 4124 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3263 3295 3263 Size: 32 4092 PspOMI G'GGCC_C Cuts at: 216 216 Size: 4124 PstI C_TGCA'G Cuts at: 761 1171 1856 761 Size: 410 685 3029 Fragments arranged by size: 3029 685 410 PvuI CG_AT'CG Cuts at: 104 104 Size: 4124 PvuII CAG'CTG Cuts at: 286 1549 1909 286 Size: 1263 360 2501 Fragments arranged by size: 2501 1263 360 RsrII CG'GwC_CG Cuts at: 2319 2319 Size: 4124 SacI G_AGCT'C Cuts at: 716 1138 716 Size: 422 3702 SalI G'TCGA_C Cuts at: 1161 1161 Size: 4124 SapI GCTCTTCn'nnn_ Cuts at: 2147 2357 2147 Size: 210 3914 SbfI CC_TGCA'GG Cuts at: 1171 1171 Size: 4124 ScaI AGT'ACT Cuts at: 886 3641 886 Size: 2755 1369 SfcI C'TryA_G Cuts at: 33 677 757 1167 1852 2845 3036 33 Size: 644 80 410 685 993 191 1121 Fragments arranged by size: 1121 993 685 644 410 191 80 SfoI GGC'GCC Cuts at: 1556 1804 1556 Size: 248 3876 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4124 SmaI CCC'GGG Cuts at: 240 1146 240 Size: 906 3218 SmlI C'TyrA_G Cuts at: 1279 2194 2686 2948 1279 Size: 915 492 262 2455 Fragments arranged by size: 2455 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4124 SphI G_CATG'C Cuts at: 1177 1677 1177 Size: 500 3624 StuI AGG'CCT Cuts at: 803 803 Size: 4124 StyI C'CwwG_G Cuts at: 107 500 638 734 953 1258 1612 107 Size: 393 138 96 219 305 354 2619 Fragments arranged by size: 2619 393 354 305 219 138 96 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1707 1840 2299 2508 1707 Size: 133 459 209 3323 Fragments arranged by size: 3323 459 209 133 TatI w'GTAC_w Cuts at: 706 884 3639 706 Size: 178 2755 1191 Fragments arranged by size: 2755 1191 178 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3641 3673 3641 Size: 32 4092 Tth111I GACn'n_nGTC Cuts at: 1921 1921 Size: 4124 XbaI T'CTAG_A Cuts at: 981 1155 981 Size: 174 3950 XcmI CCAnnnn_n'nnnnTGG Cuts at: 471 507 1619 471 Size: 36 1112 2976 Fragments arranged by size: 2976 1112 36 XmaI C'CCGG_G Cuts at: 238 1144 238 Size: 906 3218 XmnI GAAnn'nnTTC Cuts at: 1114 1114 Size: 4124 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI NspI PasI PciI PflMI PmeI PmlI PpiI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AlfI AloI AscI AvrII BaeI BcgI BfrBI BglI BmgBI BseRI BsgI BsiWI BsmI BsmBI BssHII BstEII BstZ17I ClaI CspCI DraIII EcoNI EcoRV FspAI HindIII HpaI MfeI NdeI NotI NruI NsiI PacI PfoI PpuMI PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI