Restriction map for
pF1KE0287
(Circular) (Six-base) MAPSORT of: pF1KE0287.seq Check: 2012 from: 1 to: 4478
pF1KE0287 4478 bp
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AccI GT'mk_AC
Cuts at: 1516 1516
Size: 4478
Acc65I G'GTAC_C
Cuts at: 1494 1494
Size: 4478
AclI AA'CG_TT
Cuts at: 4318 4318
Size: 4478
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 800 1361 2303 2735 3482 3750 800
Size: 561 942 432 747 268 1528
Fragments arranged by size:
1528 942 747 561 432 268
AfeI AGC'GCT
Cuts at: 1407 1407
Size: 4478
AflIII A'CryG_T
Cuts at: 626 1830 2934 3911 626
Size: 1204 1104 977 1193
Fragments arranged by size:
1204 1193 1104 977
AgeI A'CCGG_T
Cuts at: 2866 2866
Size: 4478
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 276 276
Size: 4478
AlwNI CAG_nnn'CTG
Cuts at: 1783 3350 1783
Size: 1567 2911
ApaLI G'TGCA_C
Cuts at: 3248 3248
Size: 4478
ApoI r'AATT_y
Cuts at: 1007 1482 3831 4087 1007
Size: 475 2349 256 1398
Fragments arranged by size:
2349 1398 475 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4478
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4478
AvaI C'yCGr_G
Cuts at: 1498 1498
Size: 4478
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 202 235 202
Size: 33 4445
BamHI G'GATC_C
Cuts at: 1056 1503 1056
Size: 447 4031
BanI G'GyrC_C
Cuts at: 375 1449 1467 1494 1908 2156 2191 375
Size: 1074 18 27 414 248 35 2662
Fragments arranged by size:
2662 1074 414 248 35 27 18
BanII G_rGCy'C
Cuts at: 278 1492 2522 278
Size: 1214 1030 2234
Fragments arranged by size:
2234 1214 1030
BbeI G_GCGC'C
Cuts at: 1912 2160 1912
Size: 248 4230
BciVI GTATCCnnnnn_n'
Cuts at: 2376 3137 4478 2376
Size: 761 1341 2376
Fragments arranged by size:
2376 1341 761
BclI T'GATC_A
Cuts at: 704 1187 1998 704
Size: 483 811 3184
Fragments arranged by size:
3184 811 483
BglII A'GATC_T
Cuts at: 1993 4030 1993
Size: 2037 2441
BlpI GC'TnA_GC
Cuts at: 1590 1590
Size: 4478
Bme1580I G_kGCm'C
Cuts at: 2103 2196 3252 2103
Size: 93 1056 3329
Fragments arranged by size:
3329 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1690 1771 2106 4250 1690
Size: 81 335 2144 1918
Fragments arranged by size:
2144 1918 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 592 2845 592
Size: 2253 2225
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 111 111
Size: 4478
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1654 2533 3025 3323 1654
Size: 879 492 298 2809
Fragments arranged by size:
2809 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4182 4182
Size: 4478
BsaAI yAC'GTr
Cuts at: 1831 1831
Size: 4478
BsaBI GATnn'nnATC
Cuts at: 6 1992 4029 6
Size: 1986 2037 455
Fragments arranged by size:
2037 1986 455
BsaHI Gr'CG_yC
Cuts at: 1909 2157 2859 4355 1909
Size: 248 702 1496 2032
Fragments arranged by size:
2032 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1667 2188 2866 3140 3287 3698 1667
Size: 521 678 274 147 411 2447
Fragments arranged by size:
2447 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1267 1297 1267
Size: 30 4448
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1275 1275
Size: 4478
BseYI C'CCAG_C
Cuts at: 1903 2661 3238 3734 1903
Size: 758 577 496 2647
Fragments arranged by size:
2647 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2020 2066 3274 104
Size: 1916 46 1208 1308
Fragments arranged by size:
1916 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 278 1492 2270 2460 3252 278
Size: 1214 778 190 792 1504
Fragments arranged by size:
1504 1214 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 278 1492 2103 2196 2270 2460 2522 3252 278
Size: 1214 611 93 74 190 62 730 1504
Fragments arranged by size:
1504 1214 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3698 3698
Size: 4478
BspHI T'CATG_A
Cuts at: 134 3654 134
Size: 3520 958
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1528 1742 2044 2425 1528
Size: 214 302 381 3581
Fragments arranged by size:
3581 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2770 2824 4308 4473 50
Size: 2720 54 1484 165 55
Fragments arranged by size:
2720 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 737 2390 737
Size: 1653 2825
BsrFI r'CCGG_y
Cuts at: 2476 2657 2866 2476
Size: 181 209 4088
Fragments arranged by size:
4088 209 181
BsrGI T'GTAC_A
Cuts at: 225 225
Size: 4478
BssSI C'ACGA_G
Cuts at: 2749 3107 2749
Size: 358 4120
BstAPI GCAn_nnn'nTGC
Cuts at: 1855 1855
Size: 4478
BstBI TT'CG_AA
Cuts at: 2839 2839
Size: 4478
BstYI r'GATC_y
Cuts at: 1 492 524 1056 1503 1970 1993 2328 2574
Size: 491 32 532 447 467 23 335 246
Cuts at: 2574 3586 3672 3684 4030 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 532 491 467 449 447 346 335
246 86 32 23 12
Bsu36I CC'TnA_GG
Cuts at: 640 640
Size: 4478
BtgI C'CryG_G
Cuts at: 695 695
Size: 4478
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2428 2609 2772 2428
Size: 181 163 4134
Fragments arranged by size:
4134 181 163
BtsI GCAGTG_nn'
Cuts at: 1721 1721
Size: 4478
DraI TTT'AAA
Cuts at: 198 1477 198
Size: 1279 3199
DrdI GACnn_nn'nnGTC
Cuts at: 2184 3042 2184
Size: 858 3620
EaeI y'GGCC_r
Cuts at: 2063 2237 2628 2655 4000 2063
Size: 174 391 27 1345 2541
Fragments arranged by size:
2541 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2063 2063
Size: 4478
EarI CTCTTCn'nnn_
Cuts at: 590 1429 2501 2711 590
Size: 839 1072 210 2357
Fragments arranged by size:
2357 1072 839 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2686 2996 3142 4288 2686
Size: 310 146 1146 2876
Fragments arranged by size:
2876 1146 310 146
EcoICRI GAG'CTC
Cuts at: 276 1490 276
Size: 1214 3264
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 111 800 1361 2303 2735 3482 3750 111
Size: 689 561 942 432 747 268 839
Fragments arranged by size:
942 839 747 689 561 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1617 1617
Size: 4478
EcoRI G'AATT_C
Cuts at: 1482 1482
Size: 4478
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2497 2497
Size: 4478
FspI TGC'GCA
Cuts at: 2259 2259
Size: 4478
HaeII r_GCGC'y
Cuts at: 1409 1912 2160 3182 4161 1409
Size: 503 248 1022 979 1726
Fragments arranged by size:
1726 1022 979 503 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1172 1204 2320 2350 2352 2382 1172
Size: 32 1116 30 2 30 3268
Fragments arranged by size:
3268 1116 32 30 30 2
HincII GTy'rAC
Cuts at: 609 1517 609
Size: 908 3570
KasI G'GCGC_C
Cuts at: 1908 2156 1908
Size: 248 4230
KpnI G_GTAC'C
Cuts at: 1498 1498
Size: 4478
MfeI C'AATT_G
Cuts at: 190 190
Size: 4478
MluI A'CGCG_T
Cuts at: 3911 3911
Size: 4478
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 438 1131 3149 3333 438
Size: 693 2018 184 1583
Fragments arranged by size:
2018 1583 693 184
MscI TGG'CCA
Cuts at: 2239 2239
Size: 4478
MslI CAynn'nnrTG
Cuts at: 625 700 2467 2594 625
Size: 75 1767 127 2509
Fragments arranged by size:
2509 1767 127 75
MspA1I CmG'CkG
Cuts at: 904 1589 1903 2263 3276 3521 904
Size: 685 314 360 1013 245 1861
Fragments arranged by size:
1861 1013 685 360 314 245
NaeI GCC'GGC
Cuts at: 2659 2659
Size: 4478
NarI GG'CG_CC
Cuts at: 1909 2157 1909
Size: 248 4230
NcoI C'CATG_G
Cuts at: 695 695
Size: 4478
NgoMIV G'CCGG_C
Cuts at: 2657 2657
Size: 4478
NspI r_CATG'y
Cuts at: 630 1275 1531 2031 2938 630
Size: 645 256 500 907 2170
Fragments arranged by size:
2170 907 645 500 256
PciI A'CATG_T
Cuts at: 626 2934 626
Size: 2308 2170
PflMI CCAn_nnn'nTGG
Cuts at: 520 520
Size: 4478
PmeI GTTT'AAAC
Cuts at: 1477 1477
Size: 4478
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3617 3649 3617
Size: 32 4446
PstI C_TGCA'G
Cuts at: 1361 1525 2210 1361
Size: 164 685 3629
Fragments arranged by size:
3629 685 164
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4478
PvuII CAG'CTG
Cuts at: 904 1903 2263 904
Size: 999 360 3119
Fragments arranged by size:
3119 999 360
RsrII CG'GwC_CG
Cuts at: 2673 2673
Size: 4478
SacI G_AGCT'C
Cuts at: 278 1492 278
Size: 1214 3264
SalI G'TCGA_C
Cuts at: 1515 1515
Size: 4478
SapI GCTCTTCn'nnn_
Cuts at: 590 2501 2711 590
Size: 1911 210 2357
Fragments arranged by size:
2357 1911 210
SbfI CC_TGCA'GG
Cuts at: 1525 1525
Size: 4478
ScaI AGT'ACT
Cuts at: 3995 3995
Size: 4478
SfcI C'TryA_G
Cuts at: 33 1357 1521 2206 3199 3390 33
Size: 1324 164 685 993 191 1121
Fragments arranged by size:
1324 1121 993 685 191 164
SfoI GGC'GCC
Cuts at: 1910 2158 1910
Size: 248 4230
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4478
SmaI CCC'GGG
Cuts at: 1500 1500
Size: 4478
SmlI C'TyrA_G
Cuts at: 1633 2548 3040 3302 1633
Size: 915 492 262 2809
Fragments arranged by size:
2809 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4478
SphI G_CATG'C
Cuts at: 1531 2031 1531
Size: 500 3978
SspI AAT'ATT
Cuts at: 961 1029 961
Size: 68 4410
StyI C'CwwG_G
Cuts at: 695 920 1347 1612 1966 695
Size: 225 427 265 354 3207
Fragments arranged by size:
3207 427 354 265 225
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 158 2061 2194 2653 2862 158
Size: 1903 133 459 209 1774
Fragments arranged by size:
1903 1774 459 209 133
TatI w'GTAC_w
Cuts at: 225 1274 3993 225
Size: 1049 2719 710
Fragments arranged by size:
2719 1049 710
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3995 4027 3995
Size: 32 4446
Tth111I GACn'n_nGTC
Cuts at: 2275 2275
Size: 4478
XbaI T'CTAG_A
Cuts at: 1509 1509
Size: 4478
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 785 1973 785
Size: 1188 3290
XmaI C'CCGG_G
Cuts at: 1498 1498
Size: 4478
XmnI GAAnn'nnTTC
Cuts at: 1009 1009
Size: 4478
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AloI ApaI AscI
AvrII BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsgI BsiWI BsmI BsmBI BssHII BstEII BstXI
BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI NdeI NheI NotI NruI NsiI PacI
PasI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI ZraI