Restriction map for
pF1KE0288
(Circular) (Six-base) MAPSORT of: pF1KE0288.seq Check: 6272 from: 1 to: 4052
pF1KE0288 4052 bp
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AccI GT'mk_AC
Cuts at: 713 1090 713
Size: 377 3675
Acc65I G'GTAC_C
Cuts at: 476 1068 476
Size: 592 3460
AclI AA'CG_TT
Cuts at: 3892 3892
Size: 4052
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1877 2309 3056 3324 1877
Size: 432 747 268 2605
Fragments arranged by size:
2605 747 432 268
AflIII A'CryG_T
Cuts at: 1404 2508 3485 1404
Size: 1104 977 1971
Fragments arranged by size:
1971 1104 977
AgeI A'CCGG_T
Cuts at: 2440 2440
Size: 4052
AlwNI CAG_nnn'CTG
Cuts at: 498 1357 2924 498
Size: 859 1567 1626
Fragments arranged by size:
1626 1567 859
ApaI G_GGCC'C
Cuts at: 623 623
Size: 4052
ApaLI G'TGCA_C
Cuts at: 2822 2822
Size: 4052
ApoI r'AATT_y
Cuts at: 155 1056 3405 3661 155
Size: 901 2349 256 546
Fragments arranged by size:
2349 901 546 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4052
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4052
AvaI C'yCGr_G
Cuts at: 1072 1072
Size: 4052
BamHI G'GATC_C
Cuts at: 565 1077 565
Size: 512 3540
BanI G'GyrC_C
Cuts at: 476 812 1068 1482 1730 1765 476
Size: 336 256 414 248 35 2763
Fragments arranged by size:
2763 414 336 256 248 35
BanII G_rGCy'C
Cuts at: 582 623 840 1066 2096 582
Size: 41 217 226 1030 2538
Fragments arranged by size:
2538 1030 226 217 41
BbeI G_GCGC'C
Cuts at: 816 1486 1734 816
Size: 670 248 3134
Fragments arranged by size:
3134 670 248
BciVI GTATCCnnnnn_n'
Cuts at: 173 1950 2711 4052 173
Size: 1777 761 1341 173
Fragments arranged by size:
1777 1341 761 173
BclI T'GATC_A
Cuts at: 1572 1572
Size: 4052
BglI GCCn_nnn'nGGC
Cuts at: 559 559
Size: 4052
BglII A'GATC_T
Cuts at: 1567 3604 1567
Size: 2037 2015
BlpI GC'TnA_GC
Cuts at: 524 529 1164 524
Size: 5 635 3412
Fragments arranged by size:
3412 635 5
Bme1580I G_kGCm'C
Cuts at: 623 872 1677 1770 2826 623
Size: 249 805 93 1056 1849
Fragments arranged by size:
1849 1056 805 249 93
BmrI ACTGGGnnnn_n'
Cuts at: 435 1264 1345 1680 3824 435
Size: 829 81 335 2144 663
Fragments arranged by size:
2144 829 663 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2419 2419
Size: 4052
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 568 569 568
Size: 1 4051
Bpu10I CC'TnA_GC
Cuts at: 175 175
Size: 4052
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1228 2107 2599 2897 1228
Size: 879 492 298 2383
Fragments arranged by size:
2383 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3756 3756
Size: 4052
BsaAI yAC'GTr
Cuts at: 1405 1405
Size: 4052
BsaBI GATnn'nnATC
Cuts at: 6 1566 3603 6
Size: 1560 2037 455
Fragments arranged by size:
2037 1560 455
BsaHI Gr'CG_yC
Cuts at: 813 1483 1731 2433 3929 813
Size: 670 248 702 1496 936
Fragments arranged by size:
1496 936 702 670 248
BsaWI w'CCGG_w
Cuts at: 1241 1762 2440 2714 2861 3272 1241
Size: 521 678 274 147 411 2021
Fragments arranged by size:
2021 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 827 857 827
Size: 30 4022
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 321 745 321
Size: 424 3628
BseYI C'CCAG_C
Cuts at: 225 1477 2235 2812 3308 225
Size: 1252 758 577 496 969
Fragments arranged by size:
1252 969 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1594 1640 2848 104
Size: 1490 46 1208 1308
Fragments arranged by size:
1490 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 515 1066 1844 2034 2826 515
Size: 551 778 190 792 1741
Fragments arranged by size:
1741 792 778 551 190
BsmI GAATG_Cn'
Cuts at: 1019 1019
Size: 4052
Bsp1286I G_dGCh'C
Cuts at: 515 582 623 840 872 1066 1677 1770 1844
Size: 67 41 217 32 194 611 93 74
Cuts at: 1844 2034 2096 2826 515
Size: 190 62 730 1741
Fragments arranged by size:
1741 730 611 217 194 190 93 74
67 62 41 32
BspEI T'CCGG_A
Cuts at: 3272 3272
Size: 4052
BspHI T'CATG_A
Cuts at: 353 515 3228 353
Size: 162 2713 1177
Fragments arranged by size:
2713 1177 162
BspMI ACCTGCnnnn'nnnn_
Cuts at: 242 1102 1316 1618 1999 242
Size: 860 214 302 381 2295
Fragments arranged by size:
2295 860 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2344 2398 3882 4047 50
Size: 2294 54 1484 165 55
Fragments arranged by size:
2294 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1964 1964
Size: 4052
BsrFI r'CCGG_y
Cuts at: 2050 2231 2440 2050
Size: 181 209 3662
Fragments arranged by size:
3662 209 181
BsrGI T'GTAC_A
Cuts at: 287 287
Size: 4052
BssSI C'ACGA_G
Cuts at: 2323 2681 2323
Size: 358 3694
BstAPI GCAn_nnn'nTGC
Cuts at: 443 1429 443
Size: 986 3066
BstBI TT'CG_AA
Cuts at: 2413 2413
Size: 4052
BstEII G'GTnAC_C
Cuts at: 852 852
Size: 4052
BstXI CCAn_nnnn'nTGG
Cuts at: 630 630
Size: 4052
BstYI r'GATC_y
Cuts at: 1 565 1077 1544 1567 1902 2148 3160 3246
Size: 564 512 467 23 335 246 1012 86
Cuts at: 3246 3258 3604 1
Size: 12 346 449
Fragments arranged by size:
1012 564 512 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 889 889
Size: 4052
BtgI C'CryG_G
Cuts at: 107 573 848 938 107
Size: 466 275 90 3221
Fragments arranged by size:
3221 466 275 90
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2002 2183 2346 2002
Size: 181 163 3708
Fragments arranged by size:
3708 181 163
BtsI GCAGTG_nn'
Cuts at: 665 1295 665
Size: 630 3422
DraI TTT'AAA
Cuts at: 1051 1051
Size: 4052
DraIII CAC_nnn'GTG
Cuts at: 443 443
Size: 4052
DrdI GACnn_nn'nnGTC
Cuts at: 688 1758 2616 688
Size: 1070 858 2124
Fragments arranged by size:
2124 1070 858
EaeI y'GGCC_r
Cuts at: 941 1637 1811 2202 2229 3574 941
Size: 696 174 391 27 1345 1419
Fragments arranged by size:
1419 1345 696 391 174 27
EagI C'GGCC_G
Cuts at: 1637 1637
Size: 4052
EarI CTCTTCn'nnn_
Cuts at: 2075 2285 2075
Size: 210 3842
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 592 2260 2570 2716 3862 592
Size: 1668 310 146 1146 782
Fragments arranged by size:
1668 1146 782 310 146
EcoICRI GAG'CTC
Cuts at: 1064 1064
Size: 4052
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 568 569 1877 2309 3056 3324 568
Size: 1 1308 432 747 268 1296
Fragments arranged by size:
1308 1296 747 432 268 1
EcoNI CCTnn'n_nnAGG
Cuts at: 471 471
Size: 4052
EcoO109I rG'GnC_Cy
Cuts at: 1191 1191
Size: 4052
EcoRI G'AATT_C
Cuts at: 155 1056 155
Size: 901 3151
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2071 2071
Size: 4052
FspI TGC'GCA
Cuts at: 1833 1833
Size: 4052
HaeII r_GCGC'y
Cuts at: 536 816 1486 1734 2756 3735 536
Size: 280 670 248 1022 979 853
Fragments arranged by size:
1022 979 853 670 280 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1894 1924 1926 1956 1894
Size: 30 2 30 3990
Fragments arranged by size:
3990 30 30 2
HincII GTy'rAC
Cuts at: 134 693 1091 134
Size: 559 398 3095
Fragments arranged by size:
3095 559 398
KasI G'GCGC_C
Cuts at: 812 1482 1730 812
Size: 670 248 3134
Fragments arranged by size:
3134 670 248
KpnI G_GTAC'C
Cuts at: 480 1072 480
Size: 592 3460
MluI A'CGCG_T
Cuts at: 3485 3485
Size: 4052
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2723 2907 2723
Size: 184 3868
MscI TGG'CCA
Cuts at: 1813 1813
Size: 4052
MslI CAynn'nnrTG
Cuts at: 319 460 721 2041 2168 319
Size: 141 261 1320 127 2203
Fragments arranged by size:
2203 1320 261 141 127
MspA1I CmG'CkG
Cuts at: 225 802 925 1163 1477 1837 2850 3095 225
Size: 577 123 238 314 360 1013 245 1182
Fragments arranged by size:
1182 1013 577 360 314 245 238 123
NaeI GCC'GGC
Cuts at: 2233 2233
Size: 4052
NarI GG'CG_CC
Cuts at: 813 1483 1731 813
Size: 670 248 3134
Fragments arranged by size:
3134 670 248
NcoI C'CATG_G
Cuts at: 107 573 107
Size: 466 3586
NgoMIV G'CCGG_C
Cuts at: 2231 2231
Size: 4052
NspI r_CATG'y
Cuts at: 1105 1605 2512 1105
Size: 500 907 2645
Fragments arranged by size:
2645 907 500
PciI A'CATG_T
Cuts at: 2508 2508
Size: 4052
PflMI CCAn_nnn'nTGG
Cuts at: 394 394
Size: 4052
PmeI GTTT'AAAC
Cuts at: 1051 1051
Size: 4052
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3191 3223 3191
Size: 32 4020
PspOMI G'GGCC_C
Cuts at: 619 619
Size: 4052
PstI C_TGCA'G
Cuts at: 660 1099 1784 660
Size: 439 685 2928
Fragments arranged by size:
2928 685 439
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4052
PvuII CAG'CTG
Cuts at: 225 802 925 1477 1837 225
Size: 577 123 552 360 2440
Fragments arranged by size:
2440 577 552 360 123
RsrII CG'GwC_CG
Cuts at: 2247 2247
Size: 4052
SacI G_AGCT'C
Cuts at: 1066 1066
Size: 4052
SalI G'TCGA_C
Cuts at: 1089 1089
Size: 4052
SapI GCTCTTCn'nnn_
Cuts at: 2075 2285 2075
Size: 210 3842
SbfI CC_TGCA'GG
Cuts at: 1099 1099
Size: 4052
ScaI AGT'ACT
Cuts at: 3569 3569
Size: 4052
SfcI C'TryA_G
Cuts at: 33 656 1095 1780 2773 2964 33
Size: 623 439 685 993 191 1121
Fragments arranged by size:
1121 993 685 623 439 191
SfoI GGC'GCC
Cuts at: 814 1484 1732 814
Size: 670 248 3134
Fragments arranged by size:
3134 670 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4052
SmaI CCC'GGG
Cuts at: 1074 1074
Size: 4052
SmlI C'TyrA_G
Cuts at: 1207 2122 2614 2876 1207
Size: 915 492 262 2383
Fragments arranged by size:
2383 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4052
SphI G_CATG'C
Cuts at: 1105 1605 1105
Size: 500 3552
StuI AGG'CCT
Cuts at: 894 894
Size: 4052
StyI C'CwwG_G
Cuts at: 107 265 573 1186 1540 107
Size: 158 308 613 354 2619
Fragments arranged by size:
2619 613 354 308 158
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 454 1635 1768 2227 2436 454
Size: 1181 133 459 209 2070
Fragments arranged by size:
2070 1181 459 209 133
TatI w'GTAC_w
Cuts at: 287 860 3567 287
Size: 573 2707 772
Fragments arranged by size:
2707 772 573
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3569 3601 3569
Size: 32 4020
Tth111I GACn'n_nGTC
Cuts at: 1849 1849
Size: 4052
XbaI T'CTAG_A
Cuts at: 1083 1083
Size: 4052
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 802 909 1547 802
Size: 107 638 3307
Fragments arranged by size:
3307 638 107
XmaI C'CCGG_G
Cuts at: 1072 1072
Size: 4052
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PmeI PpiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI
BmtI BsgI BsiWI BsmBI BssHII BstZ17I ClaI CspCI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PfoI PmlI PpuMI
PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI