Restriction map for
pF1KE0297
(Circular) (Six-base) MAPSORT of: pF1KE0297.seq Check: 2373 from: 1 to: 7871
pF1KE0297 7871 bp
With 182 enzymes: SgfI *
October 29, 2008 11:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1685 3348 3728 4405 1685
Size: 1663 380 677 5151
Fragments arranged by size:
5151 1663 677 380
AatII G_ACGT'C
Cuts at: 118 118
Size: 7871
AccI GT'mk_AC
Cuts at: 3569 4909 3569
Size: 1340 6531
Acc65I G'GTAC_C
Cuts at: 323 4887 323
Size: 4564 3307
AclI AA'CG_TT
Cuts at: 7711 7711
Size: 7871
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1687 2403 5696 6128 6481 7143 1687
Size: 716 3293 432 353 662 2415
Fragments arranged by size:
3293 2415 716 662 432 353
AfeI AGC'GCT
Cuts at: 3064 3064
Size: 7871
AflII C'TTAA_G
Cuts at: 580 580
Size: 7871
AflIII A'CryG_T
Cuts at: 749 2690 4463 5223 7023 7304 749
Size: 1941 1773 760 1800 281 1316
Fragments arranged by size:
1941 1800 1773 1316 760 281
AgeI A'CCGG_T
Cuts at: 2288 2288
Size: 7871
AleI CACnn'nnGTG
Cuts at: 3329 3329
Size: 7871
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1930 1948 2860 3656 3940 1930
Size: 18 912 796 284 5861
Fragments arranged by size:
5861 912 796 284 18
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4001 4033 4831 4863 4001
Size: 32 798 32 7009
Fragments arranged by size:
7009 798 32 32
AlwNI CAG_nnn'CTG
Cuts at: 130 1005 1145 2797 3780 4284 4351 4620 5176
Size: 875 140 1652 983 504 67 269 556
Cuts at: 5176 6614 130
Size: 1438 1387
Fragments arranged by size:
1652 1438 1387 983 875 556 504 269
140 67
ApaI G_GGCC'C
Cuts at: 365 2088 2171 2444 3254 3463 3894 4425 365
Size: 1723 83 273 810 209 431 531 3811
Fragments arranged by size:
3811 1723 810 531 431 273 209 83
ApaLI G'TGCA_C
Cuts at: 355 505 1582 1620 1915 6709 355
Size: 150 1077 38 295 4794 1517
Fragments arranged by size:
4794 1517 1077 295 150 38
ApoI r'AATT_y
Cuts at: 4875 7224 7480 4875
Size: 2349 256 5266
Fragments arranged by size:
5266 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7871
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7871
AvaI C'yCGr_G
Cuts at: 189 2080 2170 2391 2703 2875 3144 3253 3411
Size: 1891 90 221 312 172 269 109 158
Cuts at: 3411 3657 4231 4495 4671 4683 4891 189
Size: 246 574 264 176 12 208 3169
Fragments arranged by size:
3169 1891 574 312 269 264 246 221
208 176 172 158 109 90 12
BamHI G'GATC_C
Cuts at: 161 4896 161
Size: 4735 3136
BanI G'GyrC_C
Cuts at: 323 616 943 2632 3172 3349 3504 3562 3950
Size: 293 327 1689 540 177 155 58 388
Cuts at: 3950 3972 4386 4887 5301 5549 5584 323
Size: 22 414 501 414 248 35 2610
Fragments arranged by size:
2610 1689 540 501 414 414 388 327
293 248 177 155 58 35 22
BanII G_rGCy'C
Cuts at: 365 2059 2088 2111 2171 2444 2659 3254 3463
Size: 1694 29 23 60 273 215 595 209
Cuts at: 3463 3559 3741 3835 3874 3894 4169 4238 4425
Size: 96 182 94 39 20 275 69 187
Cuts at: 4425 4699 4816 4885 5915 365
Size: 274 117 69 1030 2321
Fragments arranged by size:
2321 1694 1030 595 275 274 273 215
209 187 182 117 96 94 69 69
60 39 29 23 20
BbeI G_GCGC'C
Cuts at: 2636 3176 3508 5305 5553 2636
Size: 540 332 1797 248 4954
Fragments arranged by size:
4954 1797 540 332 248
BbsI GAAGACnn'nnnn_
Cuts at: 1439 1675 1439
Size: 236 7635
BbvCI CC'TCA_GC
Cuts at: 123 373 550 949 1397 2226 3956 123
Size: 250 177 399 448 829 1730 4038
Fragments arranged by size:
4038 1730 829 448 399 250 177
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 285 319 1753 1787 285
Size: 34 1434 34 6369
Fragments arranged by size:
6369 1434 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3374 5769 6825 7871 3374
Size: 2395 1056 1046 3374
Fragments arranged by size:
3374 2395 1056 1046
BclI T'GATC_A
Cuts at: 5391 5391
Size: 7871
BglI GCCn_nnn'nGGC
Cuts at: 2448 3010 3229 3494 3695 4112 4154 4247 2448
Size: 562 219 265 201 417 42 93 6072
Fragments arranged by size:
6072 562 417 265 219 201 93 42
BglII A'GATC_T
Cuts at: 1365 5386 7423 1365
Size: 4021 2037 1813
Fragments arranged by size:
4021 2037 1813
BlpI GC'TnA_GC
Cuts at: 906 1129 2503 3868 4983 906
Size: 223 1374 1365 1115 3794
Fragments arranged by size:
3794 1374 1365 1115 223
Bme1580I G_kGCm'C
Cuts at: 359 365 509 948 1326 1586 1624 1919 2088
Size: 6 144 439 378 260 38 295 169
Cuts at: 2088 2130 2171 2444 3254 3352 3463 3894 3953
Size: 42 41 273 810 98 111 431 59
Cuts at: 3953 4358 4425 5496 5589 6713 359
Size: 405 67 1071 93 1124 1517
Fragments arranged by size:
1517 1124 1071 810 439 431 405 378
295 273 260 169 144 111 98 93
67 59 42 41 38 6
BmgBI CAC'GTC
Cuts at: 750 1890 1914 2034 3303 750
Size: 1140 24 120 1269 5318
Fragments arranged by size:
5318 1269 1140 120 24
BmrI ACTGGGnnnn_n'
Cuts at: 1437 2082 2920 4051 5083 5164 5499 7643 1437
Size: 645 838 1131 1032 81 335 2144 1665
Fragments arranged by size:
2144 1665 1131 1032 838 645 335 81
BmtI G_CTAG'C
Cuts at: 3689 3689
Size: 7871
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1653 3573 6238 1653
Size: 1920 2665 3286
Fragments arranged by size:
3286 2665 1920
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 187 596 747 1678 2242 2583 2595 2851 2975
Size: 409 151 931 564 341 12 256 124
Cuts at: 2975 3495 4058 4507 4642 187
Size: 520 563 449 135 3416
Fragments arranged by size:
3416 931 564 563 520 449 409 341
256 151 135 124 12
Bpu10I CC'TnA_GC
Cuts at: 123 373 550 787 949 1397 2226 3956 123
Size: 250 177 237 162 448 829 1730 4038
Fragments arranged by size:
4038 1730 829 448 250 237 177 162
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1342 2287 2302 2311 5047 5926 6640 6938 1342
Size: 945 15 9 2736 879 714 298 2275
Fragments arranged by size:
2736 2275 945 879 714 298 15 9
BsaI GGTCTCn'nnnn_
Cuts at: 1408 2177 7575 1408
Size: 769 5398 1704
Fragments arranged by size:
5398 1704 769
BsaAI yAC'GTr
Cuts at: 471 4267 5224 471
Size: 3796 957 3118
Fragments arranged by size:
3796 3118 957
BsaBI GATnn'nnATC
Cuts at: 6 4664 5385 7422 6
Size: 4658 721 2037 455
Fragments arranged by size:
4658 2037 721 455
BsaHI Gr'CG_yC
Cuts at: 115 778 827 2633 3173 3505 5302 5550 6252
Size: 663 49 1806 540 332 1797 248 702
Cuts at: 6252 7748 115
Size: 1496 238
Fragments arranged by size:
1806 1797 1496 702 663 540 332 248
238 49
BsaWI w'CCGG_w
Cuts at: 1850 2288 5060 5581 6259 6670 6817 7091 1850
Size: 438 2772 521 678 411 147 274 2630
Fragments arranged by size:
2772 2630 678 521 438 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 707 737 1504 1534 2641 2665 2671 2695 4702
Size: 30 767 30 1107 24 6 24 2007
Cuts at: 4702 4732 4798 4828 4831 4861 707
Size: 30 66 30 3 30 3717
Fragments arranged by size:
3717 2007 1107 767 66 30 30 30
30 30 24 24 6 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2105 2565 4696 4719 4852 2105
Size: 460 2131 23 133 5124
Fragments arranged by size:
5124 2131 460 133 23
BseYI C'CCAG_C
Cuts at: 954 1082 1124 1840 3406 3477 3863 3874 4129
Size: 128 42 716 1566 71 386 11 255
Cuts at: 4129 4237 4394 4520 4562 4613 4699 5296 6054
Size: 108 157 126 42 51 86 597 758
Cuts at: 6054 6719 7127 954
Size: 665 408 1698
Fragments arranged by size:
1698 1566 758 716 665 597 408 386
255 157 128 126 108 86 71 51
42 42 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1422 1521 1803 2014 2742 3079 3328 3403 3559
Size: 99 282 211 728 337 249 75 156
Cuts at: 3559 3649 4234 4294 1422
Size: 90 585 60 4999
Fragments arranged by size:
4999 728 585 337 282 249 211 156
99 90 75 60
BsiEI CG_ry'CG
Cuts at: 104 1190 1264 2640 3190 5413 5459 6689 104
Size: 1086 74 1376 550 2223 46 1230 1286
Fragments arranged by size:
2223 1376 1286 1230 1086 550 74 46
BsiHKAI G_wGCw'C
Cuts at: 359 491 509 578 1493 1586 1624 1718 1919
Size: 132 18 69 915 93 38 94 201
Cuts at: 1919 1990 2059 2659 3151 3559 4692 4816 4885
Size: 71 69 600 492 408 1133 124 69
Cuts at: 4885 5663 5853 6713 359
Size: 778 190 860 1517
Fragments arranged by size:
1517 1133 915 860 778 600 492 408
201 190 132 124 94 93 71 69
69 69 38 18
BsiWI C'GTAC_G
Cuts at: 467 467
Size: 7871
BsmBI CGTCTCn'nnnn_
Cuts at: 106 106
Size: 7871
Bsp1286I G_dGCh'C
Cuts at: 359 365 491 509 578 948 1326 1493 1586
Size: 6 126 18 69 370 378 167 93
Cuts at: 1586 1624 1718 1919 1990 2059 2088 2111 2130
Size: 38 94 201 71 69 29 23 19
Cuts at: 2130 2171 2444 2659 3151 3254 3352 3463 3559
Size: 41 273 215 492 103 98 111 96
Cuts at: 3559 3741 3835 3874 3894 3953 4169 4238 4358
Size: 182 94 39 20 59 216 69 120
Cuts at: 4358 4425 4692 4699 4816 4885 5496 5589 5663
Size: 67 267 7 117 69 611 93 74
Cuts at: 5663 5853 5915 6713 359
Size: 190 62 798 1517
Fragments arranged by size:
1517 798 611 492 378 370 273 267
216 215 201 190 182 167 126 120
117 111 103 98 96 94 94 93
93 74 71 69 69 69 69 67
62 59 41 39 38 29 23 20
19 18 7 6
BspEI T'CCGG_A
Cuts at: 1850 1850
Size: 7871
BspHI T'CATG_A
Cuts at: 6303 6303
Size: 7871
BspMI ACCTGCnnnn'nnnn_
Cuts at: 692 848 1685 3348 3728 4405 4921 5135 5437
Size: 156 837 1663 380 677 516 214 302
Cuts at: 5437 5818 692
Size: 381 2745
Fragments arranged by size:
2745 1663 837 677 516 381 380 302
214 156
BsrBI CCG'CTC
Cuts at: 50 698 2573 2609 6163 6217 7701 7866 50
Size: 648 1875 36 3554 54 1484 165 55
Fragments arranged by size:
3554 1875 1484 648 165 55 54 36
BsrDI GCAATG_nn'
Cuts at: 1914 5783 1914
Size: 3869 4002
BsrFI r'CCGG_y
Cuts at: 1263 1960 2288 2635 2754 2893 5869 6050 1263
Size: 697 328 347 119 139 2976 181 3084
Fragments arranged by size:
3084 2976 697 347 328 181 139 119
BssSI C'ACGA_G
Cuts at: 652 1717 2983 6142 6850 652
Size: 1065 1266 3159 708 1673
Fragments arranged by size:
3159 1673 1266 1065 708
BstAPI GCAn_nnn'nTGC
Cuts at: 270 1470 1572 3780 5248 270
Size: 1200 102 2208 1468 2893
Fragments arranged by size:
2893 2208 1468 1200 102
BstBI TT'CG_AA
Cuts at: 6232 6232
Size: 7871
BstEII G'GTnAC_C
Cuts at: 2493 2493
Size: 7871
BstXI CCAn_nnnn'nTGG
Cuts at: 1077 1300 3811 3990 4605 1077
Size: 223 2511 179 615 4343
Fragments arranged by size:
4343 2511 615 223 179
BstYI r'GATC_y
Cuts at: 1 161 1365 1808 4457 4896 5363 5386 5721
Size: 160 1204 443 2649 439 467 23 335
Cuts at: 5721 5967 6273 6285 6371 7423 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
2649 1204 1052 467 449 443 439 335
306 246 160 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1250 3608 1250
Size: 2358 5513
BtgI C'CryG_G
Cuts at: 107 348 743 769 986 1091 1497 1872 2015
Size: 241 395 26 217 105 406 375 143
Cuts at: 2015 2444 2464 2684 2917 2949 3052 3168 3224
Size: 429 20 220 233 32 103 116 56
Cuts at: 3224 3368 4257 4313 107
Size: 144 889 56 3665
Fragments arranged by size:
3665 889 429 406 395 375 241 233
220 217 144 143 116 105 103 56
56 32 26 20
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3229 4381 5821 6002 6165 3229
Size: 1152 1440 181 163 4935
Fragments arranged by size:
4935 1440 1152 181 163
BtsI GCAGTG_nn'
Cuts at: 128 1185 2130 2433 3547 5114 128
Size: 1057 945 303 1114 1567 2885
Fragments arranged by size:
2885 1567 1114 1057 945 303
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2124 2159 2124
Size: 35 7836
DraI TTT'AAA
Cuts at: 4870 4870
Size: 7871
DraIII CAC_nnn'GTG
Cuts at: 354 3084 354
Size: 2730 5141
DrdI GACnn_nn'nnGTC
Cuts at: 5577 6921 5577
Size: 1344 6527
EaeI y'GGCC_r
Cuts at: 911 1187 1261 2637 2946 2952 3187 3423 3578
Size: 276 74 1376 309 6 235 236 155
Cuts at: 3578 3648 3849 4248 4452 5456 5630 6021 6048
Size: 70 201 399 204 1004 174 391 27
Cuts at: 6048 7393 911
Size: 1345 1389
Fragments arranged by size:
1389 1376 1345 1004 399 391 309 276
236 235 204 201 174 155 74 70
27 6
EagI C'GGCC_G
Cuts at: 1187 1261 2637 3187 5456 1187
Size: 74 1376 550 2269 3602
Fragments arranged by size:
3602 2269 1376 550 74
EarI CTCTTCn'nnn_
Cuts at: 5894 6104 5894
Size: 210 7661
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3014 3118 3456 6079 6821 6967 7681 3014
Size: 104 338 2623 742 146 714 3204
Fragments arranged by size:
3204 2623 742 714 338 146 104
EcoICRI GAG'CTC
Cuts at: 2057 2657 3557 4814 4883 2057
Size: 600 900 1257 69 5045
Fragments arranged by size:
5045 1257 900 600 69
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 187 596 747 1678 1687 2242 2403 2583 2595
Size: 409 151 931 9 555 161 180 12
Cuts at: 2595 2851 2975 3495 4058 4507 4642 5696 6128
Size: 256 124 520 563 449 135 1054 432
Cuts at: 6128 6481 7143 187
Size: 353 662 915
Fragments arranged by size:
1054 931 915 662 563 555 520 449
432 409 353 256 180 161 151 135
124 12 9
EcoNI CCTnn'n_nnAGG
Cuts at: 611 1336 4293 4834 611
Size: 725 2957 541 3648
Fragments arranged by size:
3648 2957 725 541
EcoO109I rG'GnC_Cy
Cuts at: 362 546 681 1254 2084 2167 3250 3460 3696
Size: 184 135 573 830 83 1083 210 236
Cuts at: 3696 3890 3891 4017 4125 4421 4422 4626 5010
Size: 194 1 126 108 296 1 204 384
Cuts at: 5010 362
Size: 3223
Fragments arranged by size:
3223 1083 830 573 384 296 236 210
204 194 184 135 126 108 83 1
1
EcoRI G'AATT_C
Cuts at: 4875 4875
Size: 7871
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3660 5890 3660
Size: 2230 5641
FseI GG_CCGG'CC
Cuts at: 1267 1267
Size: 7871
FspI TGC'GCA
Cuts at: 2266 5652 2266
Size: 3386 4485
HaeII r_GCGC'y
Cuts at: 707 805 1282 2523 2636 2703 2863 3066 3176
Size: 98 477 1241 113 67 160 203 110
Cuts at: 3176 3338 3508 5305 5553 6783 7554 707
Size: 162 170 1797 248 1230 771 1024
Fragments arranged by size:
1797 1241 1230 1024 771 477 248 203
170 162 160 113 110 98 67
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1828 1860 2323 2355 2665 2697 3028 3060 3981
Size: 32 463 32 310 32 331 32 921
Cuts at: 3981 4013 4798 4830 5713 5743 5745 5775 1828
Size: 32 785 32 883 30 2 30 3924
Fragments arranged by size:
3924 921 883 785 463 331 310 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1206 4587 4910 1206
Size: 3381 323 4167
Fragments arranged by size:
4167 3381 323
KasI G'GCGC_C
Cuts at: 2632 3172 3504 5301 5549 2632
Size: 540 332 1797 248 4954
Fragments arranged by size:
4954 1797 540 332 248
KpnI G_GTAC'C
Cuts at: 327 4891 327
Size: 4564 3307
MluI A'CGCG_T
Cuts at: 2690 7304 2690
Size: 4614 3257
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1766 1808 2453 3476 6630 6814 1766
Size: 42 645 1023 3154 184 2823
Fragments arranged by size:
3154 2823 1023 645 184 42
MscI TGG'CCA
Cuts at: 2948 3580 3650 3851 5632 2948
Size: 632 70 201 1781 5187
Fragments arranged by size:
5187 1781 632 201 70
MslI CAynn'nnrTG
Cuts at: 1317 1576 1707 3329 3535 3988 5860 5987 1317
Size: 259 131 1622 206 453 1872 127 3201
Fragments arranged by size:
3201 1872 1622 453 259 206 131 127
MspA1I CmG'CkG
Cuts at: 175 226 238 377 559 771 846 910 917
Size: 51 12 139 182 212 75 64 7
Cuts at: 917 940 1347 1467 1526 1932 2248 2262 2446
Size: 23 407 120 59 406 316 14 184
Cuts at: 2446 2466 2511 2662 2726 2820 2919 3054 3099
Size: 20 45 151 64 94 99 135 45
Cuts at: 3099 3170 3734 3780 3867 4284 4982 5296 5656
Size: 71 564 46 87 417 698 314 360
Cuts at: 5656 6440 6685 175
Size: 784 245 1361
Fragments arranged by size:
1361 784 698 564 417 407 406 360
316 314 245 212 184 182 151 139
135 120 99 94 87 75 71 64
64 59 51 46 45 45 23 20
14 12 7
NaeI GCC'GGC
Cuts at: 1265 1962 2637 2756 2895 6052 1265
Size: 697 675 119 139 3157 3084
Fragments arranged by size:
3157 3084 697 675 139 119
NarI GG'CG_CC
Cuts at: 2633 3173 3505 5302 5550 2633
Size: 540 332 1797 248 4954
Fragments arranged by size:
4954 1797 540 332 248
NcoI C'CATG_G
Cuts at: 107 1091 4313 107
Size: 984 3222 3665
Fragments arranged by size:
3665 3222 984
NdeI CA'TA_TG
Cuts at: 1704 1704
Size: 7871
NgoMIV G'CCGG_C
Cuts at: 1263 1960 2635 2754 2893 6050 1263
Size: 697 675 119 139 3157 3084
Fragments arranged by size:
3157 3084 697 675 139 119
NheI G'CTAG_C
Cuts at: 3685 3685
Size: 7871
NspI r_CATG'y
Cuts at: 306 1575 1590 1861 3534 3774 3927 4440 4467
Size: 1269 15 271 1673 240 153 513 27
Cuts at: 4467 4924 5424 7027 306
Size: 457 500 1603 1150
Fragments arranged by size:
1673 1603 1269 1150 513 500 457 271
240 153 27 15
PasI CC'CwG_GG
Cuts at: 436 2212 2628 3691 3747 3758 436
Size: 1776 416 1063 56 11 4549
Fragments arranged by size:
4549 1776 1063 416 56 11
PciI A'CATG_T
Cuts at: 4463 7023 4463
Size: 2560 5311
PflMI CCAn_nnn'nTGG
Cuts at: 2016 4089 4314 4620 2016
Size: 2073 225 306 5267
Fragments arranged by size:
5267 2073 306 225
PfoI T'CCnGG_A
Cuts at: 164 2408 4619 164
Size: 2244 2211 3416
Fragments arranged by size:
3416 2244 2211
PmeI GTTT'AAAC
Cuts at: 4870 4870
Size: 7871
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4831 4863 6317 6349 4831
Size: 32 1454 32 6353
Fragments arranged by size:
6353 1454 32 32
PpuMI rG'GwC_Cy
Cuts at: 681 4017 4125 681
Size: 3336 108 4427
Fragments arranged by size:
4427 3336 108
PspOMI G'GGCC_C
Cuts at: 361 2084 2167 2440 3250 3459 3890 4421 361
Size: 1723 83 273 810 209 431 531 3811
Fragments arranged by size:
3811 1723 810 531 431 273 209 83
PspXI vC'TCGA_Gb
Cuts at: 2703 4231 4671 2703
Size: 1528 440 5903
Fragments arranged by size:
5903 1528 440
PstI C_TGCA'G
Cuts at: 225 540 845 2101 2516 3137 3725 3919 4918
Size: 315 305 1256 415 621 588 194 999
Cuts at: 4918 5603 225
Size: 685 2493
Fragments arranged by size:
2493 1256 999 685 621 588 415 315
305 194
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7871
PvuII CAG'CTG
Cuts at: 1347 1467 2262 2511 3780 3867 4284 5296 5656
Size: 120 795 249 1269 87 417 1012 360
Cuts at: 5656 1347
Size: 3562
Fragments arranged by size:
3562 1269 1012 795 417 360 249 120
87
RsrII CG'GwC_CG
Cuts at: 1752 6066 1752
Size: 4314 3557
SacI G_AGCT'C
Cuts at: 2059 2659 3559 4816 4885 2059
Size: 600 900 1257 69 5045
Fragments arranged by size:
5045 1257 900 600 69
SacII CC_GC'GG
Cuts at: 772 2447 2467 2920 3055 3171 772
Size: 1675 20 453 135 116 5472
Fragments arranged by size:
5472 1675 453 135 116 20
SalI G'TCGA_C
Cuts at: 4908 4908
Size: 7871
SanDI GG'GwC_CC
Cuts at: 4017 4017
Size: 7871
SapI GCTCTTCn'nnn_
Cuts at: 5894 6104 5894
Size: 210 7661
SbfI CC_TGCA'GG
Cuts at: 4918 4918
Size: 7871
ScaI AGT'ACT
Cuts at: 7388 7388
Size: 7871
SexAI A'CCwGG_T
Cuts at: 3284 4121 4589 3284
Size: 837 468 6566
Fragments arranged by size:
6566 837 468
SfcI C'TryA_G
Cuts at: 33 221 536 841 2097 2512 3133 3663 3679
Size: 188 315 305 1256 415 621 530 16
Cuts at: 3679 3721 3915 4193 4914 5599 6567 6758 33
Size: 42 194 278 721 685 968 191 1146
Fragments arranged by size:
1256 1146 968 721 685 621 530 415
315 305 278 194 191 188 42 16
SfoI GGC'GCC
Cuts at: 2634 3174 3506 5303 5551 2634
Size: 540 332 1797 248 4954
Fragments arranged by size:
4954 1797 540 332 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7871
SgrAI Cr'CCGG_yG
Cuts at: 2754 2754
Size: 7871
SmaI CCC'GGG
Cuts at: 191 2082 2172 2393 3413 3659 4893 191
Size: 1891 90 221 1020 246 1234 3169
Fragments arranged by size:
3169 1891 1234 1020 246 221 90
SmlI C'TyrA_G
Cuts at: 580 1357 2302 2317 2326 2703 4231 4671 5026
Size: 777 945 15 9 377 1528 440 355
Cuts at: 5026 5941 6655 6917 580
Size: 915 714 262 1534
Fragments arranged by size:
1534 1528 945 915 777 714 440 377
355 262 15 9
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7871
SphI G_CATG'C
Cuts at: 4440 4924 5424 4440
Size: 484 500 6887
Fragments arranged by size:
6887 500 484
StuI AGG'CCT
Cuts at: 202 1534 1645 2620 3125 3677 4484 4540 202
Size: 1332 111 975 505 552 807 56 3533
Fragments arranged by size:
3533 1332 975 807 552 505 111 56
StyI C'CwwG_G
Cuts at: 107 249 1091 1442 4313 4358 5005 5359 107
Size: 142 842 351 2871 45 647 354 2619
Fragments arranged by size:
2871 2619 842 647 354 351 142 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 879 1083 1138 1258 1307 1471 1792 2065 3091
Size: 204 55 120 49 164 321 273 1026
Cuts at: 3091 4070 5454 5587 6046 6255 879
Size: 979 1384 133 459 209 2495
Fragments arranged by size:
2495 1384 1026 979 459 321 273 209
204 164 133 120 55 49
TatI w'GTAC_w
Cuts at: 1568 2765 3311 7386 1568
Size: 1197 546 4075 2053
Fragments arranged by size:
4075 2053 1197 546
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 707 739 1040 1058 1072 1090 1504 1536 1666
Size: 32 301 18 14 18 414 32 130
Cuts at: 1666 1698 4702 4734 7388 7420 707
Size: 32 3004 32 2654 32 1158
Fragments arranged by size:
3004 2654 1158 414 301 130 32 32
32 32 32 18 18 14
Tth111I GACn'n_nGTC
Cuts at: 5668 5668
Size: 7871
XbaI T'CTAG_A
Cuts at: 4902 4902
Size: 7871
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 873 984 2013 3336 3496 3844 4009 4029 4365
Size: 111 1029 1323 160 348 165 20 336
Cuts at: 4365 5366 873
Size: 1001 3378
Fragments arranged by size:
3378 1323 1029 1001 348 336 165 160
111 20
XhoI C'TCGA_G
Cuts at: 2703 4231 4671 2703
Size: 1528 440 5903
Fragments arranged by size:
5903 1528 440
XmaI C'CCGG_G
Cuts at: 189 2080 2170 2391 3411 3657 4891 189
Size: 1891 90 221 1020 246 1234 3169
Fragments arranged by size:
3169 1891 1234 1020 246 221 90
XmnI GAAnn'nnTTC
Cuts at: 1555 1555
Size: 7871
ZraI GAC'GTC
Cuts at: 116 116
Size: 7871
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PspXI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AhdI AscI AvrII BaeI BfrBI BsmI BsrGI BssHII
BstZ17I ClaI EcoRV FspAI HindIII HpaI MfeI NotI
NruI NsiI PacI PmlI PshAI PsiI PsrI SfiI
SnaBI SrfI SspI SwaI