Restriction map for
pF1KE0299
(Circular) (Six-base) MAPSORT of: pF1KE0299.seq Check: 7875 from: 1 to: 18542
pF1KE0299 18542 bp
With 182 enzymes: SgfI *
January 27, 2010 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1338 3084 3495 1338
Size: 1746 411 16385
Fragments arranged by size:
16385 1746 411
AatII G_ACGT'C
Cuts at: 4986 4986
Size: 18542
AccI GT'mk_AC
Cuts at: 3087 8851 11206 12612 15580 3087
Size: 5764 2355 1406 2968 6049
Fragments arranged by size:
6049 5764 2968 2355 1406
Acc65I G'GTAC_C
Cuts at: 15558 15558
Size: 18542
AclI AA'CG_TT
Cuts at: 18382 18382
Size: 18542
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 737 781 2110 2240 2489 2907 3707 3773 3794
Size: 44 1329 130 249 418 800 66 21
Cuts at: 3794 3815 3857 3944 4799 4844 5252 5411 6599
Size: 21 42 87 855 45 408 159 1188
Cuts at: 6599 6971 7736 7994 8144 9173 9179 9368 9707
Size: 372 765 258 150 1029 6 189 339
Cuts at: 9707 9746 10172 10187 13094 13205 13328 15134 15379
Size: 39 426 15 2907 111 123 1806 245
Cuts at: 15379 16367 16799 17546 17814 737
Size: 988 432 747 268 1465
Fragments arranged by size:
2907 1806 1465 1329 1188 1029 988 855
800 765 747 432 426 418 408 372
339 268 258 249 245 189 159 150
130 123 111 87 66 45 44 42
39 21 21 15 6
AfeI AGC'GCT
Cuts at: 9799 9799
Size: 18542
AflIII A'CryG_T
Cuts at: 5996 11609 14385 15894 16998 17975 5996
Size: 5613 2776 1509 1104 977 6563
Fragments arranged by size:
6563 5613 2776 1509 1104 977
AgeI A'CCGG_T
Cuts at: 16930 16930
Size: 18542
AhdI GACnn_n'nnGTC
Cuts at: 7208 9239 7208
Size: 2031 16511
AleI CACnn'nnGTG
Cuts at: 9451 9451
Size: 18542
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2309 2341 10074 10106 14042 14074 2309
Size: 32 7733 32 3936 32 6777
Fragments arranged by size:
7733 6777 3936 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 172 303 652 1795 2503 3076 3121 4822 6964
Size: 131 349 1143 708 573 45 1701 2142
Cuts at: 6964 9115 9277 9580 10029 10621 10777 13897 13914
Size: 2151 162 303 449 592 156 3120 17
Cuts at: 13914 14851 15112 15847 17414 172
Size: 937 261 735 1567 1300
Fragments arranged by size:
3120 2151 2142 1701 1567 1300 1143 937
735 708 592 573 449 349 303 261
162 156 131 45 17
ApaI G_GGCC'C
Cuts at: 2994 2994
Size: 18542
ApaLI G'TGCA_C
Cuts at: 17312 17312
Size: 18542
ApoI r'AATT_y
Cuts at: 622 2937 3659 4235 6563 6763 6782 7740 7997
Size: 2315 722 576 2328 200 19 958 257
Cuts at: 7997 9102 9833 11698 12502 12715 12871 13179 13790
Size: 1105 731 1865 804 213 156 308 611
Cuts at: 13790 13902 14201 15197 15226 15546 17895 18151 622
Size: 112 299 996 29 320 2349 256 1013
Fragments arranged by size:
2349 2328 2315 1865 1105 1013 996 958
804 731 722 611 576 320 308 299
257 256 213 200 156 112 29 19
AseI AT'TA_AT
Cuts at: 20 20
Size: 18542
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 18542
AvaI C'yCGr_G
Cuts at: 5340 13343 13680 14273 14952 15562 5340
Size: 8003 337 593 679 610 8320
Fragments arranged by size:
8320 8003 679 610 593 337
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 5291 5324 6236 6269 8453 8486 10508 10541 5291
Size: 33 912 33 2184 33 2022 33 13292
Fragments arranged by size:
13292 2184 2022 912 33 33 33 33
BamHI G'GATC_C
Cuts at: 789 3321 12459 14083 15567 789
Size: 2532 9138 1624 1484 3764
Fragments arranged by size:
9138 3764 2532 1624 1484
BanI G'GyrC_C
Cuts at: 1404 2196 15558 15972 16220 16255 1404
Size: 792 13362 414 248 35 3691
Fragments arranged by size:
13362 3691 792 414 248 35
BanII G_rGCy'C
Cuts at: 143 202 2043 2994 3137 5123 5556 5660 8609
Size: 59 1841 951 143 1986 433 104 2949
Cuts at: 8609 9770 11196 11259 12026 15556 16586 143
Size: 1161 1426 63 767 3530 1030 2099
Fragments arranged by size:
3530 2949 2099 1986 1841 1426 1161 1030
951 767 433 143 104 63 59
BbeI G_GCGC'C
Cuts at: 15976 16224 15976
Size: 248 18294
BbsI GAAGACnn'nnnn_
Cuts at: 405 2098 3845 4745 4986 5009 5999 7802 12401
Size: 1693 1747 900 241 23 990 1803 4599
Cuts at: 12401 12452 15175 405
Size: 51 2723 3772
Fragments arranged by size:
4599 3772 2723 1803 1747 1693 990 900
241 51 23
BbvCI CC'TCA_GC
Cuts at: 1725 5849 7658 9806 11594 1725
Size: 4124 1809 2148 1788 8673
Fragments arranged by size:
8673 4124 2148 1809 1788
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1736 1770 10027 10061 13116 13150 1736
Size: 34 8257 34 3055 34 7128
Fragments arranged by size:
8257 7128 3055 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 8612 8781 10288 10509 14588 16440 17201 18542 8612
Size: 169 1507 221 4079 1852 761 1341 8612
Fragments arranged by size:
8612 4079 1852 1507 1341 761 221 169
BclI T'GATC_A
Cuts at: 4095 6885 7128 12225 14607 15240 15302 16062 4095
Size: 2790 243 5097 2382 633 62 760 6575
Fragments arranged by size:
6575 5097 2790 2382 760 633 243 62
BfrBI ATG'CAT
Cuts at: 940 5614 5874 13102 940
Size: 4674 260 7228 6380
Fragments arranged by size:
7228 6380 4674 260
BglI GCCn_nnn'nGGC
Cuts at: 169 1116 169
Size: 947 17595
BglII A'GATC_T
Cuts at: 5665 7941 9180 10052 16057 18094 5665
Size: 2276 1239 872 6005 2037 6113
Fragments arranged by size:
6113 6005 2276 2037 1239 872
BlpI GC'TnA_GC
Cuts at: 250 1106 1196 1214 1226 1283 1593 1713 1760
Size: 856 90 18 12 57 310 120 47
Cuts at: 1760 3221 8603 9971 11498 12927 15654 250
Size: 1461 5382 1368 1527 1429 2727 3138
Fragments arranged by size:
5382 3138 2727 1527 1461 1429 1368 856
310 120 90 57 47 18 12
Bme1580I G_kGCm'C
Cuts at: 2994 10734 11344 12907 16167 16260 17316 2994
Size: 7740 610 1563 3260 93 1056 4220
Fragments arranged by size:
7740 4220 3260 1563 1056 610 93
BmrI ACTGGGnnnn_n'
Cuts at: 1156 1244 2609 2900 7000 7040 9327 9936 12910
Size: 88 1365 291 4100 40 2287 609 2974
Cuts at: 12910 15754 15835 16170 18314 1156
Size: 2844 81 335 2144 1384
Fragments arranged by size:
4100 2974 2844 2287 2144 1384 1365 609
335 291 88 81 40
BmtI G_CTAG'C
Cuts at: 188 188
Size: 18542
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 221 10601 11596 16909 221
Size: 10380 995 5313 1854
Fragments arranged by size:
10380 5313 1854 995
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 194 845 1034 1175 1205 1292 1313 1379 1409
Size: 651 189 141 30 87 21 66 30
Cuts at: 1409 2045 3274 5105 6121 7217 7433 8054 8189
Size: 636 1229 1831 1016 1096 216 621 135
Cuts at: 8189 8669 9799 10051 10376 10635 11273 11383 11566
Size: 480 1130 252 325 259 638 110 183
Cuts at: 11566 12789 12972 13978 14491 194
Size: 1223 183 1006 513 4245
Fragments arranged by size:
4245 1831 1229 1223 1130 1096 1016 1006
651 638 636 621 513 480 325 259
252 216 189 183 183 141 135 110
87 66 30 30 21
Bpu10I CC'TnA_GC
Cuts at: 767 1400 1725 2201 5849 7571 7616 7658 9806
Size: 633 325 476 3648 1722 45 42 2148
Cuts at: 9806 10282 11594 13973 767
Size: 476 1312 2379 5336
Fragments arranged by size:
5336 3648 2379 2148 1722 1312 633 476
476 325 45 42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2861 3893 4137 4258 4358 10009 11909 12162 12359
Size: 1032 244 121 100 5651 1900 253 197
Cuts at: 12359 13049 14440 15718 16597 17089 17387 2861
Size: 690 1391 1278 879 492 298 4016
Fragments arranged by size:
5651 4016 1900 1391 1278 1032 879 690
492 298 253 244 197 121 100
BsaI GGTCTCn'nnnn_
Cuts at: 439 2875 7195 7489 8182 12377 12734 18246 439
Size: 2436 4320 294 693 4195 357 5512 735
Fragments arranged by size:
5512 4320 4195 2436 735 693 357 294
BsaAI yAC'GTr
Cuts at: 5999 15895 5999
Size: 9896 8646
BsaBI GATnn'nnATC
Cuts at: 6 10541 12224 14502 16056 18093 6
Size: 10535 1683 2278 1554 2037 455
Fragments arranged by size:
10535 2278 2037 1683 1554 455
BsaHI Gr'CG_yC
Cuts at: 417 4983 15973 16221 16923 18419 417
Size: 4566 10990 248 702 1496 540
Fragments arranged by size:
10990 4566 1496 702 540 248
BsaWI w'CCGG_w
Cuts at: 15731 16252 16930 17204 17351 17762 15731
Size: 521 678 274 147 411 16511
Fragments arranged by size:
16511 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 882 912 971 1001 1173 1203 1407 1437 1497
Size: 30 59 30 172 30 204 30 60
Cuts at: 1497 1527 1809 1839 1841 1871 2469 2499 7539
Size: 30 282 30 2 30 598 30 5040
Cuts at: 7539 7569 8187 8217 8715 8745 9877 9907 10074
Size: 30 618 30 498 30 1132 30 167
Cuts at: 10074 10104 10349 10379 14234 14264 14892 14922 882
Size: 30 245 30 3855 30 628 30 4502
Fragments arranged by size:
5040 4502 3855 1132 628 618 598 498
282 245 204 172 167 60 59 30
30 30 30 30 30 30 30 30
30 30 30 30 30 30 30 2
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 191 194 274 279 504 890 2477 2864 4519
Size: 3 80 5 225 386 1587 387 1655
Cuts at: 4519 4777 5102 5105 5668 5866 7313 7322 7337
Size: 258 325 3 563 198 1447 9 15
Cuts at: 7337 7340 7343 7346 7364 7547 9898 10048 11314
Size: 3 3 3 18 183 2351 150 1266
Cuts at: 11314 14940 191
Size: 3626 3793
Fragments arranged by size:
3793 3626 2351 1655 1587 1447 1266 563
387 386 325 258 225 198 183 150
80 18 15 9 5 3 3 3
3 3
BseYI C'CCAG_C
Cuts at: 1101 1343 1490 1533 1563 1653 1743 2078 3114
Size: 242 147 43 30 90 90 335 1036
Cuts at: 3114 9277 11106 13907 14098 15967 16725 17302 17798
Size: 6163 1829 2801 191 1869 758 577 496
Cuts at: 17798 1101
Size: 1845
Fragments arranged by size:
6163 2801 1869 1845 1829 1036 758 577
496 335 242 191 147 90 90 43
30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2081 2156 3473 7535 8573 8952 2081
Size: 75 1317 4062 1038 379 11671
Fragments arranged by size:
11671 4062 1317 1038 379 75
BsiEI CG_ry'CG
Cuts at: 104 164 224 329 16084 16130 17338 104
Size: 60 60 105 15755 46 1208 1308
Fragments arranged by size:
15755 1308 1208 105 60 60 46
BsiHKAI G_wGCw'C
Cuts at: 551 2043 5123 5556 5660 9711 9770 10288 15556
Size: 1492 3080 433 104 4051 59 518 5268
Cuts at: 15556 16334 16524 17316 551
Size: 778 190 792 1777
Fragments arranged by size:
5268 4051 3080 1777 1492 792 778 518
433 190 104 59
BsmI GAATG_Cn'
Cuts at: 3355 6413 9147 13366 14358 14891 3355
Size: 3058 2734 4219 992 533 7006
Fragments arranged by size:
7006 4219 3058 2734 992 533
BsmBI CGTCTCn'nnnn_
Cuts at: 6455 9631 9955 12349 6455
Size: 3176 324 2394 12648
Fragments arranged by size:
12648 3176 2394 324
Bsp1286I G_dGCh'C
Cuts at: 143 202 551 2043 2994 3137 5123 5556 5660
Size: 59 349 1492 951 143 1986 433 104
Cuts at: 5660 8609 9711 9770 10288 10734 11196 11259 11344
Size: 2949 1102 59 518 446 462 63 85
Cuts at: 11344 12026 12907 15556 16167 16260 16334 16524 16586
Size: 682 881 2649 611 93 74 190 62
Cuts at: 16586 17316 143
Size: 730 1369
Fragments arranged by size:
2949 2649 1986 1492 1369 1102 951 881
730 682 611 518 462 446 433 349
190 143 104 93 85 74 63 62
59 59
BspEI T'CCGG_A
Cuts at: 17762 17762
Size: 18542
BspHI T'CATG_A
Cuts at: 9947 12315 13923 17718 9947
Size: 2368 1608 3795 10771
Fragments arranged by size:
10771 3795 2368 1608
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1338 2586 3084 3495 7230 9510 12962 14703 15592
Size: 1248 498 411 3735 2280 3452 1741 889
Cuts at: 15592 15806 16108 16489 1338
Size: 214 302 381 3391
Fragments arranged by size:
3735 3452 3391 2280 1741 1248 889 498
411 381 302 214
BsrBI CCG'CTC
Cuts at: 50 9933 16834 16888 18372 18537 50
Size: 9883 6901 54 1484 165 55
Fragments arranged by size:
9883 6901 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 7858 8570 9569 10262 13300 16454 7858
Size: 712 999 693 3038 3154 9946
Fragments arranged by size:
9946 3154 3038 999 712 693
BsrFI r'CCGG_y
Cuts at: 223 12368 14918 14944 16540 16721 16930 223
Size: 12145 2550 26 1596 181 209 1835
Fragments arranged by size:
12145 2550 1835 1596 209 181 26
BsrGI T'GTAC_A
Cuts at: 10443 10443
Size: 18542
BssSI C'ACGA_G
Cuts at: 10586 10733 11384 16813 17171 10586
Size: 147 651 5429 358 11957
Fragments arranged by size:
11957 5429 651 358 147
BstAPI GCAn_nnn'nTGC
Cuts at: 303 480 3350 6327 8560 14851 15919 303
Size: 177 2870 2977 2233 6291 1068 2926
Fragments arranged by size:
6291 2977 2926 2870 2233 1068 177
BstBI TT'CG_AA
Cuts at: 4776 11451 12351 16903 4776
Size: 6675 900 4552 6415
Fragments arranged by size:
6675 6415 4552 900
BstEII G'GTnAC_C
Cuts at: 2729 3672 2729
Size: 943 17599
BstXI CCAn_nnnn'nTGG
Cuts at: 1498 3252 7944 11379 1498
Size: 1754 4692 3435 8661
Fragments arranged by size:
8661 4692 3435 1754
BstYI r'GATC_y
Cuts at: 1 789 1792 2052 2184 3321 5254 5665 6111
Size: 788 1003 260 132 1137 1933 411 446
Cuts at: 6111 6343 7941 8802 9180 9995 10052 11360 12324
Size: 232 1598 861 378 815 57 1308 964
Cuts at: 12324 12459 12880 14050 14083 15477 15567 16034 16057
Size: 135 421 1170 33 1394 90 467 23
Cuts at: 16057 16392 16638 17650 17736 17748 18094 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1933 1598 1394 1308 1170 1137 1012 1003
964 861 815 788 467 449 446 421
411 378 346 335 260 246 232 135
132 90 86 57 33 23 12
BstZ17I GTA'TAC
Cuts at: 3088 11207 3088
Size: 8119 10423
Bsu36I CC'TnA_GG
Cuts at: 1113 2279 2801 6182 8999 11297 1113
Size: 1166 522 3381 2817 2298 8358
Fragments arranged by size:
8358 3381 2817 2298 1166 522
BtgI C'CryG_G
Cuts at: 107 6392 11998 14444 14679 107
Size: 6285 5606 2446 235 3970
Fragments arranged by size:
6285 5606 3970 2446 235
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 16492 16673 16836 16492
Size: 181 163 18198
Fragments arranged by size:
18198 181 163
BtsI GCAGTG_nn'
Cuts at: 997 2126 2948 3560 4725 6477 8702 9494 14976
Size: 1129 822 612 1165 1752 2225 792 5482
Cuts at: 14976 15005 15785 997
Size: 29 780 3754
Fragments arranged by size:
5482 3754 2225 1752 1165 1129 822 792
780 612 29
ClaI AT'CG_AT
Cuts at: 9042 9042
Size: 18542
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2385 2420 13922 13957 2385
Size: 35 11502 35 6970
Fragments arranged by size:
11502 6970 35 35
DraI TTT'AAA
Cuts at: 539 3428 4545 5109 10422 15541 539
Size: 2889 1117 564 5313 5119 3540
Fragments arranged by size:
5313 5119 3540 2889 1117 564
DraIII CAC_nnn'GTG
Cuts at: 1943 2980 4231 1943
Size: 1037 1251 16254
Fragments arranged by size:
16254 1251 1037
DrdI GACnn_nn'nnGTC
Cuts at: 12369 13529 16248 17106 12369
Size: 1160 2719 858 13805
Fragments arranged by size:
13805 2719 1160 858
EaeI y'GGCC_r
Cuts at: 161 221 326 2763 8477 13212 13229 16127 16301
Size: 60 105 2437 5714 4735 17 2898 174
Cuts at: 16301 16692 16719 18064 161
Size: 391 27 1345 639
Fragments arranged by size:
5714 4735 2898 2437 1345 639 391 174
105 60 27 17
EagI C'GGCC_G
Cuts at: 161 221 326 16127 161
Size: 60 105 15801 2576
Fragments arranged by size:
15801 2576 105 60
EarI CTCTTCn'nnn_
Cuts at: 251 539 2465 3689 3734 4496 4620 4643 4754
Size: 288 1926 1224 45 762 124 23 111
Cuts at: 4754 5186 5621 6066 6310 6702 7268 8082 8913
Size: 432 435 445 244 392 566 814 831
Cuts at: 8913 8999 9923 11787 11798 13050 15398 16565 16775
Size: 86 924 1864 11 1252 2348 1167 210
Cuts at: 16775 251
Size: 2018
Fragments arranged by size:
2348 2018 1926 1864 1252 1224 1167 924
831 814 762 566 445 435 432 392
288 244 210 124 111 86 45 23
11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 250 12454 16750 17060 17206 18352 250
Size: 12204 4296 310 146 1146 440
Fragments arranged by size:
12204 4296 1146 440 310 146
EcoICRI GAG'CTC
Cuts at: 2041 5121 5554 5658 9768 15554 2041
Size: 3080 433 104 4110 5786 5029
Fragments arranged by size:
5786 5029 4110 3080 433 104
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 194 737 781 845 1034 1175 1205 1292 1313
Size: 543 44 64 189 141 30 87 21
Cuts at: 1313 1379 1409 2045 2110 2240 2489 2907 3274
Size: 66 30 636 65 130 249 418 367
Cuts at: 3274 3707 3773 3794 3815 3857 3944 4799 4844
Size: 433 66 21 21 42 87 855 45
Cuts at: 4844 5105 5252 5411 6121 6599 6971 7217 7433
Size: 261 147 159 710 478 372 246 216
Cuts at: 7433 7736 7994 8054 8144 8189 8669 9173 9179
Size: 303 258 60 90 45 480 504 6
Cuts at: 9179 9368 9707 9746 9799 10051 10172 10187 10376
Size: 189 339 39 53 252 121 15 189
Cuts at: 10376 10635 11273 11383 11566 12789 12972 13094 13205
Size: 259 638 110 183 1223 183 122 111
Cuts at: 13205 13328 13978 14491 15134 15379 16367 16799 17546
Size: 123 650 513 643 245 988 432 747
Cuts at: 17546 17814 194
Size: 268 922
Fragments arranged by size:
1223 988 922 855 747 710 650 643
638 636 543 513 504 480 478 433
432 418 372 367 339 303 268 261
259 258 252 249 246 245 216 189
189 189 183 183 159 147 141 130
123 122 121 111 110 90 87 87
66 66 65 64 60 53 45 45
44 42 39 30 30 21 21 21
15 6
EcoNI CCTnn'n_nnAGG
Cuts at: 580 1442 1850 11548 14521 580
Size: 862 408 9698 2973 4601
Fragments arranged by size:
9698 4601 2973 862 408
EcoO109I rG'GnC_Cy
Cuts at: 778 886 1471 2346 2990 2991 3499 6360 7002
Size: 108 585 875 644 1 508 2861 642
Cuts at: 7002 9328 11541 14068 14117 15681 778
Size: 2326 2213 2527 49 1564 3639
Fragments arranged by size:
3639 2861 2527 2326 2213 1564 875 644
642 585 508 108 49 1
EcoRI G'AATT_C
Cuts at: 11698 13179 13790 15546 11698
Size: 1481 611 1756 14694
Fragments arranged by size:
14694 1756 1481 611
EcoRV GAT'ATC
Cuts at: 644 9591 10682 644
Size: 8947 1091 8504
Fragments arranged by size:
8947 8504 1091
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3436 3949 4331 4649 5563 6860 7189 7316 11485
Size: 513 382 318 914 1297 329 127 4169
Cuts at: 11485 13052 14332 16561 3436
Size: 1567 1280 2229 5417
Fragments arranged by size:
5417 4169 2229 1567 1297 1280 914 513
382 329 318 127
FspI TGC'GCA
Cuts at: 16323 16323
Size: 18542
HaeII r_GCGC'y
Cuts at: 9801 15976 16224 17246 18225 9801
Size: 6175 248 1022 979 10118
Fragments arranged by size:
10118 6175 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 538 570 628 660 1296 1328 1443 1475 2278
Size: 32 58 32 636 32 115 32 803
Cuts at: 2278 2310 2392 2424 2616 2648 2903 2935 3193
Size: 32 82 32 192 32 255 32 258
Cuts at: 3193 3225 4126 4158 4201 4233 4384 4416 5788
Size: 32 901 32 43 32 151 32 1372
Cuts at: 5788 5820 6901 6933 7194 7226 9225 9257 9877
Size: 32 1081 32 261 32 1999 32 620
Cuts at: 9877 9909 9954 9986 10349 10381 10392 10424 11596
Size: 32 45 32 363 32 11 32 1172
Cuts at: 11596 11628 12513 12545 13862 13894 14321 14353 16384
Size: 32 885 32 1317 32 427 32 2031
Cuts at: 16384 16414 16416 16446 538
Size: 30 2 30 2634
Fragments arranged by size:
2634 2031 1999 1372 1317 1172 1081 901
885 803 636 620 427 363 261 258
255 192 151 115 82 58 45 43
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
30 30 11 2
HincII GTy'rAC
Cuts at: 588 2646 7436 13534 15498 15581 588
Size: 2058 4790 6098 1964 83 3549
Fragments arranged by size:
6098 4790 3549 2058 1964 83
HindIII A'AGCT_T
Cuts at: 3719 3931 5894 6162 7061 12475 13753 3719
Size: 212 1963 268 899 5414 1278 8508
Fragments arranged by size:
8508 5414 1963 1278 899 268 212
HpaI GTT'AAC
Cuts at: 588 7436 588
Size: 6848 11694
KasI G'GCGC_C
Cuts at: 15972 16220 15972
Size: 248 18294
KpnI G_GTAC'C
Cuts at: 15562 15562
Size: 18542
MfeI C'AATT_G
Cuts at: 5114 10202 13166 5114
Size: 5088 2964 10490
Fragments arranged by size:
10490 5088 2964
MluI A'CGCG_T
Cuts at: 17975 17975
Size: 18542
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 347 1278 1363 3178 4276 5644 7064 7690 7826
Size: 931 85 1815 1098 1368 1420 626 136
Cuts at: 7826 7906 8445 8604 9421 9860 10023 10683 11194
Size: 80 539 159 817 439 163 660 511
Cuts at: 11194 11776 13410 14531 17213 17397 347
Size: 582 1634 1121 2682 184 1492
Fragments arranged by size:
2682 1815 1634 1492 1420 1368 1121 1098
931 817 660 626 582 539 511 439
184 163 159 136 85 80
MscI TGG'CCA
Cuts at: 2765 8479 13214 13231 16303 2765
Size: 5714 4735 17 3072 5004
Fragments arranged by size:
5714 5004 4735 3072 17
MslI CAynn'nnrTG
Cuts at: 4792 9451 12428 16531 16658 4792
Size: 4659 2977 4103 127 6676
Fragments arranged by size:
6676 4659 4103 2977 127
MspA1I CmG'CkG
Cuts at: 169 249 280 331 2761 3121 3220 5773 5905
Size: 80 31 51 2430 360 99 2553 132
Cuts at: 5905 7504 7990 10005 10029 10192 10944 12289 13243
Size: 1599 486 2015 24 163 752 1345 954
Cuts at: 13243 14022 15653 15967 16327 17340 17585 169
Size: 779 1631 314 360 1013 245 1126
Fragments arranged by size:
2553 2430 2015 1631 1599 1345 1126 1013
954 779 752 486 360 360 314 245
163 132 99 80 51 31 24
NaeI GCC'GGC
Cuts at: 225 16723 225
Size: 16498 2044
NarI GG'CG_CC
Cuts at: 15973 16221 15973
Size: 248 18294
NcoI C'CATG_G
Cuts at: 107 6392 14444 14679 107
Size: 6285 8052 235 3970
Fragments arranged by size:
8052 6285 3970 235
NdeI CA'TA_TG
Cuts at: 9054 13812 9054
Size: 4758 13784
NgoMIV G'CCGG_C
Cuts at: 223 16721 223
Size: 16498 2044
NheI G'CTAG_C
Cuts at: 184 184
Size: 18542
NotI GC'GGCC_GC
Cuts at: 161 161
Size: 18542
NsiI A_TGCA'T
Cuts at: 942 5616 5876 13104 942
Size: 4674 260 7228 6380
Fragments arranged by size:
7228 6380 4674 260
NspI r_CATG'y
Cuts at: 1909 1937 5272 8507 8563 8602 8695 11613 13393
Size: 28 3335 3235 56 39 93 2918 1780
Cuts at: 13393 14389 14712 15538 15595 16095 17002 1909
Size: 996 323 826 57 500 907 3449
Fragments arranged by size:
3449 3335 3235 2918 1780 996 907 826
500 323 93 57 56 39 28
PasI CC'CwG_GG
Cuts at: 412 8775 13454 13960 14104 412
Size: 8363 4679 506 144 4850
Fragments arranged by size:
8363 4850 4679 506 144
PciI A'CATG_T
Cuts at: 11609 14385 16998 11609
Size: 2776 2613 13153
Fragments arranged by size:
13153 2776 2613
PflMI CCAn_nnn'nTGG
Cuts at: 1039 1350 6868 10029 11901 13221 13914 1039
Size: 311 5518 3161 1872 1320 693 5667
Fragments arranged by size:
5667 5518 3161 1872 1320 693 311
PfoI T'CCnGG_A
Cuts at: 1038 1290 9770 11350 11399 1038
Size: 252 8480 1580 49 8181
Fragments arranged by size:
8480 8181 1580 252 49
PmeI GTTT'AAAC
Cuts at: 15541 15541
Size: 18542
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 10074 10106 17681 17713 10074
Size: 32 7575 32 10903
Fragments arranged by size:
10903 7575 32 32
PpuMI rG'GwC_Cy
Cuts at: 778 886 1471 3499 7002 9328 14068 14117 778
Size: 108 585 2028 3503 2326 4740 49 5203
Fragments arranged by size:
5203 4740 3503 2326 2028 585 108 49
PshAI GACnn'nnGTC
Cuts at: 8497 8497
Size: 18542
PsiI TTA'TAA
Cuts at: 5759 7763 8434 8945 9544 14432 5759
Size: 2004 671 511 599 4888 9869
Fragments arranged by size:
9869 4888 2004 671 599 511
PspOMI G'GGCC_C
Cuts at: 2990 2990
Size: 18542
PspXI vC'TCGA_Gb
Cuts at: 13343 13343
Size: 18542
PstI C_TGCA'G
Cuts at: 1811 2955 5154 5646 9893 11368 12204 12938 13242
Size: 1144 2199 492 4247 1475 836 734 304
Cuts at: 13242 13269 14928 15012 15384 15589 16274 1811
Size: 27 1659 84 372 205 685 4079
Fragments arranged by size:
4247 4079 2199 1659 1475 1144 836 734
685 492 372 304 205 84 27
PvuI CG_AT'CG
Cuts at: 104 104
Size: 18542
PvuII CAG'CTG
Cuts at: 249 3121 3220 5773 5905 7504 7990 10005 10029
Size: 2872 99 2553 132 1599 486 2015 24
Cuts at: 10029 10192 10944 13243 15967 16327 249
Size: 163 752 2299 2724 360 2464
Fragments arranged by size:
2872 2724 2553 2464 2299 2015 1599 752
486 360 163 132 99 24
RsrII CG'GwC_CG
Cuts at: 16737 16737
Size: 18542
SacI G_AGCT'C
Cuts at: 2043 5123 5556 5660 9770 15556 2043
Size: 3080 433 104 4110 5786 5029
Fragments arranged by size:
5786 5029 4110 3080 433 104
SalI G'TCGA_C
Cuts at: 15579 15579
Size: 18542
SanDI GG'GwC_CC
Cuts at: 14117 14117
Size: 18542
SapI GCTCTTCn'nnn_
Cuts at: 539 3734 5186 6702 8913 9923 11798 13050 16565
Size: 3195 1452 1516 2211 1010 1875 1252 3515
Cuts at: 16565 16775 539
Size: 210 2306
Fragments arranged by size:
3515 3195 2306 2211 1875 1516 1452 1252
1010 210
SbfI CC_TGCA'GG
Cuts at: 15384 15589 15384
Size: 205 18337
ScaI AGT'ACT
Cuts at: 4872 11078 11779 18059 4872
Size: 6206 701 6280 5355
Fragments arranged by size:
6280 6206 5355 701
SexAI A'CCwGG_T
Cuts at: 429 780 8630 10272 429
Size: 351 7850 1642 8699
Fragments arranged by size:
8699 7850 1642 351
SfcI C'TryA_G
Cuts at: 33 452 1387 1807 1959 2951 3527 5150 5642
Size: 419 935 420 152 992 576 1623 492
Cuts at: 5642 6260 6935 7457 7970 7991 8528 8642 9203
Size: 618 675 522 513 21 537 114 561
Cuts at: 9203 9236 9889 10103 11364 12200 12573 12934 13238
Size: 33 653 214 1261 836 373 361 304
Cuts at: 13238 13265 14008 14924 15008 15380 15585 16270 17263
Size: 27 743 916 84 372 205 685 993
Cuts at: 17263 17454 33
Size: 191 1121
Fragments arranged by size:
1623 1261 1121 993 992 935 916 836
743 685 675 653 618 576 561 537
522 513 492 420 419 373 372 361
304 214 205 191 152 114 84 33
27 21
SfoI GGC'GCC
Cuts at: 15974 16222 15974
Size: 248 18294
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 18542
SmaI CCC'GGG
Cuts at: 13682 15564 13682
Size: 1882 16660
SmlI C'TyrA_G
Cuts at: 2876 3908 4152 4273 4373 9988 11924 12141 12374
Size: 1032 244 121 100 5615 1936 217 233
Cuts at: 12374 13064 13343 14273 14455 15697 16612 17104 17366
Size: 690 279 930 182 1242 915 492 262
Cuts at: 17366 2876
Size: 4052
Fragments arranged by size:
5615 4052 1936 1242 1032 930 915 690
492 279 262 244 233 217 182 121
100
SpeI A'CTAG_T
Cuts at: 67 2223 4699 11742 67
Size: 2156 2476 7043 6867
Fragments arranged by size:
7043 6867 2476 2156
SphI G_CATG'C
Cuts at: 5272 8602 15595 16095 5272
Size: 3330 6993 500 7719
Fragments arranged by size:
7719 6993 3330 500
SspI AAT'ATT
Cuts at: 5146 5683 12855 15216 15282 5146
Size: 537 7172 2361 66 8406
Fragments arranged by size:
8406 7172 2361 537 66
StuI AGG'CCT
Cuts at: 948 1856 7614 7712 948
Size: 908 5758 98 11778
Fragments arranged by size:
11778 5758 908 98
StyI C'CwwG_G
Cuts at: 107 308 2192 2261 4179 4481 4746 6392 7922
Size: 201 1884 69 1918 302 265 1646 1530
Cuts at: 7922 10221 10745 14444 14517 14569 14679 15676 16030
Size: 2299 524 3699 73 52 110 997 354
Cuts at: 16030 107
Size: 2619
Fragments arranged by size:
3699 2619 2299 1918 1884 1646 1530 997
524 354 302 265 201 110 73 69
52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 768 3060 6666 6717 7148 8539 10884 10974 11001
Size: 2292 3606 51 431 1391 2345 90 27
Cuts at: 11001 11823 13505 13654 16125 16258 16717 16926 768
Size: 822 1682 149 2471 133 459 209 2384
Fragments arranged by size:
3606 2471 2384 2345 2292 1682 1391 822
459 431 209 149 133 90 51 27
TatI w'GTAC_w
Cuts at: 2454 3912 4348 4381 4870 5128 5745 7543 8158
Size: 1458 436 33 489 258 617 1798 615
Cuts at: 8158 8515 8809 10259 10443 10938 11076 11777 12125
Size: 357 294 1450 184 495 138 701 348
Cuts at: 12125 14366 18057 2454
Size: 2241 3691 2939
Fragments arranged by size:
3691 2939 2241 1798 1458 1450 701 617
615 495 489 436 357 348 294 258
184 138 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1841 1873 6470 6502 8610 8642 8715 8747 9802
Size: 32 4597 32 2108 32 73 32 1055
Cuts at: 9802 9834 10262 10294 10572 10587 10604 10619 10824
Size: 32 428 32 278 15 17 15 205
Cuts at: 10824 10856 18059 18091 1841
Size: 32 7203 32 2292
Fragments arranged by size:
7203 4597 2292 2108 1055 428 278 205
73 32 32 32 32 32 32 32
32 17 15 15
Tth111I GACn'n_nGTC
Cuts at: 1236 6384 8632 13481 16339 1236
Size: 5148 2248 4849 2858 3439
Fragments arranged by size:
5148 4849 3439 2858 2248
XbaI T'CTAG_A
Cuts at: 7491 8220 12415 13080 13519 15573 7491
Size: 729 4195 665 439 2054 10460
Fragments arranged by size:
10460 4195 2054 729 665 439
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1792 6364 6711 7920 10941 14743 16037 1792
Size: 4572 347 1209 3021 3802 1294 4297
Fragments arranged by size:
4572 4297 3802 3021 1294 1209 347
XhoI C'TCGA_G
Cuts at: 13343 14273 13343
Size: 930 17612
XmaI C'CCGG_G
Cuts at: 13680 15562 13680
Size: 1882 16660
XmnI GAAnn'nnTTC
Cuts at: 1482 3659 5511 7296 9680 10086 12050 13820 15359
Size: 2177 1852 1785 2384 406 1964 1770 1539
Cuts at: 15359 1482
Size: 4665
Fragments arranged by size:
4665 2384 2177 1964 1852 1785 1770 1539
406
ZraI GAC'GTC
Cuts at: 4984 4984
Size: 18542
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AflII AlfI AscI AvrII BmgBI BsiWI BssHII FseI
FspAI NruI PacI PmlI PsrI SacII SfiI SgrAI
SnaBI SrfI SwaI