Restriction map for
pF1KE0301
(Circular) (Six-base) MAPSORT of: pF1KE0301.seq Check: 9431 from: 1 to: 4640
pF1KE0301 4640 bp
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 111 111
Size: 4640
AccI GT'mk_AC
Cuts at: 1678 1678
Size: 4640
Acc65I G'GTAC_C
Cuts at: 1656 1656
Size: 4640
AclI AA'CG_TT
Cuts at: 1634 4480 1634
Size: 2846 1794
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 907 2465 2897 3644 3912 907
Size: 1558 432 747 268 1635
Fragments arranged by size:
1635 1558 747 432 268
AflIII A'CryG_T
Cuts at: 1268 1398 1992 3096 4073 1268
Size: 130 594 1104 977 1835
Fragments arranged by size:
1835 1104 977 594 130
AgeI A'CCGG_T
Cuts at: 3028 3028
Size: 4640
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1069 1069
Size: 4640
AlwNI CAG_nnn'CTG
Cuts at: 126 340 610 736 1504 1945 3512 126
Size: 214 270 126 768 441 1567 1254
Fragments arranged by size:
1567 1254 768 441 270 214 126
ApaLI G'TGCA_C
Cuts at: 3410 3410
Size: 4640
ApoI r'AATT_y
Cuts at: 1644 3993 4249 1644
Size: 2349 256 2035
Fragments arranged by size:
2349 2035 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4640
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4640
AvaI C'yCGr_G
Cuts at: 955 1552 1660 955
Size: 597 108 3935
Fragments arranged by size:
3935 597 108
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 482 515 482
Size: 33 4607
BamHI G'GATC_C
Cuts at: 1665 1665
Size: 4640
BanI G'GyrC_C
Cuts at: 1656 2070 2318 2353 1656
Size: 414 248 35 3943
Fragments arranged by size:
3943 414 248 35
BanII G_rGCy'C
Cuts at: 1654 2684 1654
Size: 1030 3610
BbeI G_GCGC'C
Cuts at: 2074 2322 2074
Size: 248 4392
BbsI GAAGACnn'nnnn_
Cuts at: 1206 1206
Size: 4640
BciVI GTATCCnnnnn_n'
Cuts at: 483 643 2538 3299 4640 483
Size: 160 1895 761 1341 483
Fragments arranged by size:
1895 1341 761 483 160
BclI T'GATC_A
Cuts at: 2160 2160
Size: 4640
BglII A'GATC_T
Cuts at: 2155 4192 2155
Size: 2037 2603
BlpI GC'TnA_GC
Cuts at: 1752 1752
Size: 4640
Bme1580I G_kGCm'C
Cuts at: 552 2265 2358 3414 552
Size: 1713 93 1056 1778
Fragments arranged by size:
1778 1713 1056 93
BmgBI CAC'GTC
Cuts at: 264 264
Size: 4640
BmrI ACTGGGnnnn_n'
Cuts at: 156 1116 1852 1933 2268 4412 156
Size: 960 736 81 335 2144 384
Fragments arranged by size:
2144 960 736 384 335 81
BmtI G_CTAG'C
Cuts at: 460 460
Size: 4640
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3007 3007
Size: 4640
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 545 686 545
Size: 141 4499
Bpu10I CC'TnA_GC
Cuts at: 461 461
Size: 4640
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1816 2695 3187 3485 1816
Size: 879 492 298 2971
Fragments arranged by size:
2971 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4344 4344
Size: 4640
BsaAI yAC'GTr
Cuts at: 906 1993 906
Size: 1087 3553
BsaBI GATnn'nnATC
Cuts at: 6 2154 4191 6
Size: 2148 2037 455
Fragments arranged by size:
2148 2037 455
BsaHI Gr'CG_yC
Cuts at: 2071 2319 3021 4517 2071
Size: 248 702 1496 2194
Fragments arranged by size:
2194 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1149 1829 2350 3028 3302 3449 3860 1149
Size: 680 521 678 274 147 411 1929
Fragments arranged by size:
1929 680 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1370 1370
Size: 4640
BseYI C'CCAG_C
Cuts at: 452 2065 2823 3400 3896 452
Size: 1613 758 577 496 1196
Fragments arranged by size:
1613 1196 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 190
Size: 4640
BsiEI CG_ry'CG
Cuts at: 104 2182 2228 3436 104
Size: 2078 46 1208 1308
Fragments arranged by size:
2078 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 486 1654 2432 2622 3414 486
Size: 1168 778 190 792 1712
Fragments arranged by size:
1712 1168 792 778 190
BsmI GAATG_Cn'
Cuts at: 696 696
Size: 4640
BsmBI CGTCTCn'nnnn_
Cuts at: 205 205
Size: 4640
Bsp1286I G_dGCh'C
Cuts at: 486 552 1654 2265 2358 2432 2622 2684 3414
Size: 66 1102 611 93 74 190 62 730
Cuts at: 3414 486
Size: 1712
Fragments arranged by size:
1712 1102 730 611 190 93 74 66
62
BspEI T'CCGG_A
Cuts at: 3860 3860
Size: 4640
BspHI T'CATG_A
Cuts at: 1049 3816 1049
Size: 2767 1873
BspMI ACCTGCnnnn'nnnn_
Cuts at: 111 1690 1904 2206 2587 111
Size: 1579 214 302 381 2164
Fragments arranged by size:
2164 1579 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2932 2986 4470 4635 50
Size: 2882 54 1484 165 55
Fragments arranged by size:
2882 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1097 2552 1097
Size: 1455 3185
BsrFI r'CCGG_y
Cuts at: 2638 2819 3028 2638
Size: 181 209 4250
Fragments arranged by size:
4250 209 181
BsrGI T'GTAC_A
Cuts at: 1265 1265
Size: 4640
BssSI C'ACGA_G
Cuts at: 2911 3269 2911
Size: 358 4282
BstAPI GCAn_nnn'nTGC
Cuts at: 126 2017 126
Size: 1891 2749
BstBI TT'CG_AA
Cuts at: 3001 3001
Size: 4640
BstYI r'GATC_y
Cuts at: 1 791 831 1290 1665 2132 2155 2490 2736
Size: 790 40 459 375 467 23 335 246
Cuts at: 2736 3748 3834 3846 4192 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 790 467 459 449 375 346 335
246 86 40 23 12
Bsu36I CC'TnA_GG
Cuts at: 1185 1185
Size: 4640
BtgI C'CryG_G
Cuts at: 827 827
Size: 4640
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2590 2771 2934 2590
Size: 181 163 4296
Fragments arranged by size:
4296 181 163
BtsI GCAGTG_nn'
Cuts at: 1883 1883
Size: 4640
DraI TTT'AAA
Cuts at: 1639 1639
Size: 4640
DrdI GACnn_nn'nnGTC
Cuts at: 526 2346 3204 526
Size: 1820 858 1962
Fragments arranged by size:
1962 1820 858
EaeI y'GGCC_r
Cuts at: 311 2225 2399 2790 2817 4162 311
Size: 1914 174 391 27 1345 789
Fragments arranged by size:
1914 1345 789 391 174 27
EagI C'GGCC_G
Cuts at: 2225 2225
Size: 4640
EarI CTCTTCn'nnn_
Cuts at: 257 2663 2873 257
Size: 2406 210 2024
Fragments arranged by size:
2406 2024 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 196 2848 3158 3304 4450 196
Size: 2652 310 146 1146 386
Fragments arranged by size:
2652 1146 386 310 146
EcoICRI GAG'CTC
Cuts at: 1652 1652
Size: 4640
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 545 686 907 2465 2897 3644 3912 545
Size: 141 221 1558 432 747 268 1273
Fragments arranged by size:
1558 1273 747 432 268 221 141
EcoO109I rG'GnC_Cy
Cuts at: 158 1016 1779 158
Size: 858 763 3019
Fragments arranged by size:
3019 858 763
EcoRI G'AATT_C
Cuts at: 1644 1644
Size: 4640
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2659 2659
Size: 4640
FspI TGC'GCA
Cuts at: 2421 2421
Size: 4640
HaeII r_GCGC'y
Cuts at: 2074 2322 3344 4323 2074
Size: 248 1022 979 2391
Fragments arranged by size:
2391 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2482 2512 2514 2544 2482
Size: 30 2 30 4578
Fragments arranged by size:
4578 30 30 2
HincII GTy'rAC
Cuts at: 978 1274 1679 978
Size: 296 405 3939
Fragments arranged by size:
3939 405 296
HpaI GTT'AAC
Cuts at: 978 978
Size: 4640
KasI G'GCGC_C
Cuts at: 2070 2318 2070
Size: 248 4392
KpnI G_GTAC'C
Cuts at: 1660 1660
Size: 4640
MluI A'CGCG_T
Cuts at: 4073 4073
Size: 4640
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1386 3311 3495 1386
Size: 1925 184 2531
Fragments arranged by size:
2531 1925 184
MscI TGG'CCA
Cuts at: 313 2401 313
Size: 2088 2552
MslI CAynn'nnrTG
Cuts at: 1051 2629 2756 1051
Size: 1578 127 2935
Fragments arranged by size:
2935 1578 127
MspA1I CmG'CkG
Cuts at: 415 969 1174 1751 2065 2425 3438 3683 415
Size: 554 205 577 314 360 1013 245 1372
Fragments arranged by size:
1372 1013 577 554 360 314 245 205
NaeI GCC'GGC
Cuts at: 2821 2821
Size: 4640
NarI GG'CG_CC
Cuts at: 2071 2319 2071
Size: 248 4392
NdeI CA'TA_TG
Cuts at: 502 502
Size: 4640
NgoMIV G'CCGG_C
Cuts at: 2819 2819
Size: 4640
NheI G'CTAG_C
Cuts at: 456 456
Size: 4640
NspI r_CATG'y
Cuts at: 1272 1402 1693 2193 3100 1272
Size: 130 291 500 907 2812
Fragments arranged by size:
2812 907 500 291 130
PciI A'CATG_T
Cuts at: 1268 1398 3096 1268
Size: 130 1698 2812
Fragments arranged by size:
2812 1698 130
PfoI T'CCnGG_A
Cuts at: 635 635
Size: 4640
PmeI GTTT'AAAC
Cuts at: 1639 1639
Size: 4640
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3779 3811 3779
Size: 32 4608
PpuMI rG'GwC_Cy
Cuts at: 158 1016 158
Size: 858 3782
PsiI TTA'TAA
Cuts at: 320 320
Size: 4640
PstI C_TGCA'G
Cuts at: 221 543 1173 1604 1687 2372 221
Size: 322 630 431 83 685 2489
Fragments arranged by size:
2489 685 630 431 322 83
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4640
PvuII CAG'CTG
Cuts at: 415 969 1174 2065 2425 415
Size: 554 205 891 360 2630
Fragments arranged by size:
2630 891 554 360 205
RsrII CG'GwC_CG
Cuts at: 2835 2835
Size: 4640
SacI G_AGCT'C
Cuts at: 1654 1654
Size: 4640
SalI G'TCGA_C
Cuts at: 1677 1677
Size: 4640
SapI GCTCTTCn'nnn_
Cuts at: 2663 2873 2663
Size: 210 4430
SbfI CC_TGCA'GG
Cuts at: 1687 1687
Size: 4640
ScaI AGT'ACT
Cuts at: 626 4157 626
Size: 3531 1109
SexAI A'CCwGG_T
Cuts at: 1503 1503
Size: 4640
SfcI C'TryA_G
Cuts at: 33 217 539 1169 1600 1683 2368 3361 3552
Size: 184 322 630 431 83 685 993 191
Cuts at: 3552 33
Size: 1121
Fragments arranged by size:
1121 993 685 630 431 322 191 184
83
SfoI GGC'GCC
Cuts at: 2072 2320 2072
Size: 248 4392
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4640
SmaI CCC'GGG
Cuts at: 1662 1662
Size: 4640
SmlI C'TyrA_G
Cuts at: 1795 2710 3202 3464 1795
Size: 915 492 262 2971
Fragments arranged by size:
2971 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4640
SphI G_CATG'C
Cuts at: 1693 2193 1693
Size: 500 4140
SspI AAT'ATT
Cuts at: 1389 1389
Size: 4640
StyI C'CwwG_G
Cuts at: 1774 2128 1774
Size: 354 4286
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2223 2356 2815 3024 2223
Size: 133 459 209 3839
Fragments arranged by size:
3839 459 209 133
TatI w'GTAC_w
Cuts at: 624 873 1265 4155 624
Size: 249 392 2890 1109
Fragments arranged by size:
2890 1109 392 249
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4157 4189 4157
Size: 32 4608
Tth111I GACn'n_nGTC
Cuts at: 265 2437 265
Size: 2172 2468
XbaI T'CTAG_A
Cuts at: 1671 1671
Size: 4640
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2135 2135
Size: 4640
XmaI C'CCGG_G
Cuts at: 1660 1660
Size: 4640
XmnI GAAnn'nnTTC
Cuts at: 397 1364 397
Size: 967 3673
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HpaI KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NdeI NgoMIV NheI
NspI PciI PfoI PmeI PpiI PpuMI PsiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BbvCI BcgI BfrBI BglI BsaXI BsiWI BssHII
BstEII BstXI BstZ17I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII MfeI NcoI NotI NruI NsiI
PacI PasI PflMI PmlI PshAI PspOMI PspXI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI ZraI