(Circular) (Six-base) MAPSORT of: pF1KE0303.seq Check: 5464 from: 1 to: 4901 pF1KE0303 4901 bp With 182 enzymes: SgfI * April 24, 2009 13:12 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 162 162 Size: 4901 AccI GT'mk_AC Cuts at: 1939 1939 Size: 4901 Acc65I G'GTAC_C Cuts at: 1917 1917 Size: 4901 AclI AA'CG_TT Cuts at: 1895 4741 1895 Size: 2846 2055 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 932 2726 3158 3905 4173 932 Size: 1794 432 747 268 1660 Fragments arranged by size: 1794 1660 747 432 268 AfeI AGC'GCT Cuts at: 1102 1102 Size: 4901 AflIII A'CryG_T Cuts at: 2253 3357 4334 2253 Size: 1104 977 2820 Fragments arranged by size: 2820 1104 977 AgeI A'CCGG_T Cuts at: 3289 3289 Size: 4901 AleI CACnn'nnGTG Cuts at: 590 661 739 590 Size: 71 78 4752 Fragments arranged by size: 4752 78 71 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1418 1418 Size: 4901 AlwNI CAG_nnn'CTG Cuts at: 1585 2206 3773 1585 Size: 621 1567 2713 Fragments arranged by size: 2713 1567 621 ApaLI G'TGCA_C Cuts at: 1356 3671 1356 Size: 2315 2586 ApoI r'AATT_y Cuts at: 1905 4254 4510 1905 Size: 2349 256 2296 Fragments arranged by size: 2349 2296 256 AseI AT'TA_AT Cuts at: 20 847 20 Size: 827 4074 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4901 AvaI C'yCGr_G Cuts at: 1211 1921 1211 Size: 710 4191 BamHI G'GATC_C Cuts at: 1764 1926 1764 Size: 162 4739 BanI G'GyrC_C Cuts at: 163 769 1917 2331 2579 2614 163 Size: 606 1148 414 248 35 2450 Fragments arranged by size: 2450 1148 606 414 248 35 BanII G_rGCy'C Cuts at: 238 1915 2945 238 Size: 1677 1030 2194 Fragments arranged by size: 2194 1677 1030 BbeI G_GCGC'C Cuts at: 2335 2583 2335 Size: 248 4653 BbvCI CC'TCA_GC Cuts at: 119 119 Size: 4901 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 426 460 426 Size: 34 4867 BciVI GTATCCnnnnn_n' Cuts at: 2799 3560 4901 2799 Size: 761 1341 2799 Fragments arranged by size: 2799 1341 761 BclI T'GATC_A Cuts at: 1221 2421 1221 Size: 1200 3701 BfrBI ATG'CAT Cuts at: 1883 1883 Size: 4901 BglII A'GATC_T Cuts at: 2416 4453 2416 Size: 2037 2864 BlpI GC'TnA_GC Cuts at: 1007 2013 1007 Size: 1006 3895 Bme1580I G_kGCm'C Cuts at: 166 772 1360 1808 2526 2619 3675 166 Size: 606 588 448 718 93 1056 1392 Fragments arranged by size: 1392 1056 718 606 588 448 93 BmgBI CAC'GTC Cuts at: 681 681 Size: 4901 BmrI ACTGGGnnnn_n' Cuts at: 415 2113 2194 2529 4673 415 Size: 1698 81 335 2144 643 Fragments arranged by size: 2144 1698 643 335 81 BmtI G_CTAG'C Cuts at: 1190 1190 Size: 4901 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1163 3268 1163 Size: 2105 2796 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1342 1653 1342 Size: 311 4590 Bpu10I CC'TnA_GC Cuts at: 119 1097 1575 119 Size: 978 478 3445 Fragments arranged by size: 3445 978 478 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2077 2956 3448 3746 2077 Size: 879 492 298 3232 Fragments arranged by size: 3232 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 4605 4605 Size: 4901 BsaAI yAC'GTr Cuts at: 2254 2254 Size: 4901 BsaBI GATnn'nnATC Cuts at: 6 2415 4452 6 Size: 2409 2037 455 Fragments arranged by size: 2409 2037 455 BsaHI Gr'CG_yC Cuts at: 2332 2580 3282 4778 2332 Size: 248 702 1496 2455 Fragments arranged by size: 2455 1496 702 248 BsaWI w'CCGG_w Cuts at: 330 1229 2090 2611 3289 3563 3710 4121 330 Size: 899 861 521 678 274 147 411 1110 Fragments arranged by size: 1110 899 861 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 676 706 676 Size: 30 4871 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 157 157 Size: 4901 BseYI C'CCAG_C Cuts at: 1011 2326 3084 3661 4157 1011 Size: 1315 758 577 496 1755 Fragments arranged by size: 1755 1315 758 577 496 BsiEI CG_ry'CG Cuts at: 104 342 675 2443 2489 3697 104 Size: 238 333 1768 46 1208 1308 Fragments arranged by size: 1768 1308 1208 333 238 46 BsiHKAI G_wGCw'C Cuts at: 238 611 1360 1915 2693 2883 3675 238 Size: 373 749 555 778 190 792 1464 Fragments arranged by size: 1464 792 778 749 555 373 190 BsmBI CGTCTCn'nnnn_ Cuts at: 1389 1671 1761 1389 Size: 282 90 4529 Fragments arranged by size: 4529 282 90 Bsp1286I G_dGCh'C Cuts at: 166 238 611 772 1360 1808 1915 2526 2619 Size: 72 373 161 588 448 107 611 93 Cuts at: 2619 2693 2883 2945 3675 166 Size: 74 190 62 730 1392 Fragments arranged by size: 1392 730 611 588 448 373 190 161 107 93 74 72 62 BspEI T'CCGG_A Cuts at: 330 1229 4121 330 Size: 899 2892 1110 Fragments arranged by size: 2892 1110 899 BspHI T'CATG_A Cuts at: 299 476 4077 299 Size: 177 3601 1123 Fragments arranged by size: 3601 1123 177 BspMI ACCTGCnnnn'nnnn_ Cuts at: 162 1951 2165 2467 2848 162 Size: 1789 214 302 381 2215 Fragments arranged by size: 2215 1789 381 302 214 BsrBI CCG'CTC Cuts at: 50 3193 3247 4731 4896 50 Size: 3143 54 1484 165 55 Fragments arranged by size: 3143 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 524 595 1175 2813 524 Size: 71 580 1638 2612 Fragments arranged by size: 2612 1638 580 71 BsrFI r'CCGG_y Cuts at: 2899 3080 3289 2899 Size: 181 209 4511 Fragments arranged by size: 4511 209 181 BssSI C'ACGA_G Cuts at: 231 3172 3530 231 Size: 2941 358 1602 Fragments arranged by size: 2941 1602 358 BstAPI GCAn_nnn'nTGC Cuts at: 1417 2278 1417 Size: 861 4040 BstBI TT'CG_AA Cuts at: 1597 3262 1597 Size: 1665 3236 BstXI CCAn_nnnn'nTGG Cuts at: 741 741 Size: 4901 BstYI r'GATC_y Cuts at: 1 318 1764 1926 2393 2416 2751 2997 4009 Size: 317 1446 162 467 23 335 246 1012 Cuts at: 4009 4095 4107 4453 1 Size: 86 12 346 449 Fragments arranged by size: 1446 1012 467 449 346 335 317 246 162 86 23 12 BtgI C'CryG_G Cuts at: 107 1164 1388 1760 107 Size: 1057 224 372 3248 Fragments arranged by size: 3248 1057 372 224 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2851 3032 3195 2851 Size: 181 163 4557 Fragments arranged by size: 4557 181 163 BtsI GCAGTG_nn' Cuts at: 609 734 1799 2144 609 Size: 125 1065 345 3366 Fragments arranged by size: 3366 1065 345 125 DraI TTT'AAA Cuts at: 1900 1900 Size: 4901 DrdI GACnn_nn'nnGTC Cuts at: 346 2607 3465 346 Size: 2261 858 1782 Fragments arranged by size: 2261 1782 858 EaeI y'GGCC_r Cuts at: 188 240 672 746 1871 2486 2660 3051 3078 Size: 52 432 74 1125 615 174 391 27 Cuts at: 3078 4423 188 Size: 1345 666 Fragments arranged by size: 1345 1125 666 615 432 391 174 74 52 27 EagI C'GGCC_G Cuts at: 672 2486 672 Size: 1814 3087 EarI CTCTTCn'nnn_ Cuts at: 2924 3134 2924 Size: 210 4691 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 127 1504 3109 3419 3565 4711 127 Size: 1377 1605 310 146 1146 317 Fragments arranged by size: 1605 1377 1146 317 310 146 EcoICRI GAG'CTC Cuts at: 236 1913 236 Size: 1677 3224 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 932 1342 1653 2726 3158 3905 4173 932 Size: 410 311 1073 432 747 268 1660 Fragments arranged by size: 1660 1073 747 432 410 311 268 EcoO109I rG'GnC_Cy Cuts at: 1509 2040 1509 Size: 531 4370 EcoRI G'AATT_C Cuts at: 1905 1905 Size: 4901 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2920 2920 Size: 4901 FspI TGC'GCA Cuts at: 1749 2682 1749 Size: 933 3968 HaeII r_GCGC'y Cuts at: 636 1104 2335 2583 3605 4584 636 Size: 468 1231 248 1022 979 953 Fragments arranged by size: 1231 1022 979 953 468 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 262 294 335 367 475 507 927 959 963 Size: 32 41 32 108 32 420 32 4 Cuts at: 963 995 1369 1401 2743 2773 2775 2805 262 Size: 32 374 32 1342 30 2 30 2358 Fragments arranged by size: 2358 1342 420 374 108 41 32 32 32 32 32 32 30 30 4 2 HincII GTy'rAC Cuts at: 918 1940 918 Size: 1022 3879 KasI G'GCGC_C Cuts at: 2331 2579 2331 Size: 248 4653 KpnI G_GTAC'C Cuts at: 1921 1921 Size: 4901 MluI A'CGCG_T Cuts at: 4334 4334 Size: 4901 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 3572 3756 3572 Size: 184 4717 MscI TGG'CCA Cuts at: 190 748 2662 190 Size: 558 1914 2429 Fragments arranged by size: 2429 1914 558 MslI CAynn'nnrTG Cuts at: 280 590 661 739 1702 2890 3017 280 Size: 310 71 78 963 1188 127 2164 Fragments arranged by size: 2164 1188 963 310 127 78 71 MspA1I CmG'CkG Cuts at: 1582 2012 2326 2686 3699 3944 1582 Size: 430 314 360 1013 245 2539 Fragments arranged by size: 2539 1013 430 360 314 245 NaeI GCC'GGC Cuts at: 3082 3082 Size: 4901 NarI GG'CG_CC Cuts at: 2332 2580 2332 Size: 248 4653 NcoI C'CATG_G Cuts at: 107 1760 107 Size: 1653 3248 NgoMIV G'CCGG_C Cuts at: 3080 3080 Size: 4901 NheI G'CTAG_C Cuts at: 1186 1186 Size: 4901 NsiI A_TGCA'T Cuts at: 1885 1885 Size: 4901 NspI r_CATG'y Cuts at: 227 419 805 992 1399 1887 1954 2454 3361 Size: 192 386 187 407 488 67 500 907 Cuts at: 3361 227 Size: 1767 Fragments arranged by size: 1767 907 500 488 407 386 192 187 67 PciI A'CATG_T Cuts at: 3357 3357 Size: 4901 PfoI T'CCnGG_A Cuts at: 137 137 Size: 4901 PmeI GTTT'AAAC Cuts at: 1900 1900 Size: 4901 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4040 4072 4040 Size: 32 4869 PpuMI rG'GwC_Cy Cuts at: 1509 1509 Size: 4901 PspXI vC'TCGA_Gb Cuts at: 1211 1211 Size: 4901 PstI C_TGCA'G Cuts at: 1948 2633 1948 Size: 685 4216 PvuI CG_AT'CG Cuts at: 104 104 Size: 4901 PvuII CAG'CTG Cuts at: 1582 2326 2686 1582 Size: 744 360 3797 Fragments arranged by size: 3797 744 360 RsrII CG'GwC_CG Cuts at: 3096 3096 Size: 4901 SacI G_AGCT'C Cuts at: 238 1915 238 Size: 1677 3224 SalI G'TCGA_C Cuts at: 1938 1938 Size: 4901 SapI GCTCTTCn'nnn_ Cuts at: 2924 3134 2924 Size: 210 4691 SbfI CC_TGCA'GG Cuts at: 1948 1948 Size: 4901 ScaI AGT'ACT Cuts at: 442 4418 442 Size: 3976 925 SexAI A'CCwGG_T Cuts at: 1054 1054 Size: 4901 SfcI C'TryA_G Cuts at: 33 537 1944 2629 3622 3813 33 Size: 504 1407 685 993 191 1121 Fragments arranged by size: 1407 1121 993 685 504 191 SfoI GGC'GCC Cuts at: 2333 2581 2333 Size: 248 4653 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4901 SmaI CCC'GGG Cuts at: 1923 1923 Size: 4901 SmlI C'TyrA_G Cuts at: 1211 2056 2971 3463 3725 1211 Size: 845 915 492 262 2387 Fragments arranged by size: 2387 915 845 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4901 SphI G_CATG'C Cuts at: 805 992 1399 1954 2454 805 Size: 187 407 555 500 3252 Fragments arranged by size: 3252 555 500 407 187 StuI AGG'CCT Cuts at: 1241 1241 Size: 4901 StyI C'CwwG_G Cuts at: 107 1421 1505 1760 2035 2389 107 Size: 1314 84 255 275 354 2619 Fragments arranged by size: 2619 1314 354 275 255 84 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 356 638 2484 2617 3076 3285 356 Size: 282 1846 133 459 209 1972 Fragments arranged by size: 1972 1846 459 282 209 133 TatI w'GTAC_w Cuts at: 440 4416 440 Size: 3976 925 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 676 708 1547 1579 4418 4450 676 Size: 32 839 32 2839 32 1127 Fragments arranged by size: 2839 1127 839 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1534 2698 1534 Size: 1164 3737 XbaI T'CTAG_A Cuts at: 1932 1932 Size: 4901 XcmI CCAnnnn_n'nnnnTGG Cuts at: 741 1644 2396 741 Size: 903 752 3246 Fragments arranged by size: 3246 903 752 XhoI C'TCGA_G Cuts at: 1211 1211 Size: 4901 XmaI C'CCGG_G Cuts at: 1921 1921 Size: 4901 XmnI GAAnn'nnTTC Cuts at: 207 207 Size: 4901 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI NsiI NspI PciI PfoI PmeI PpiI PpuMI PspXI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI Enzymes that do not cut: AatII AflII AhdI AloI ApaI AscI AvrII BaeI BbsI BglI BsgI BsiWI BsmI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NotI NruI PacI PasI PflMI PmlI PshAI PsiI PspOMI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SspI SwaI ZraI