Restriction map for
pF1KE0304
(Circular) (Six-base) MAPSORT of: pF1KE0304.seq Check: 1681 from: 1 to: 4580
pF1KE0304 4580 bp
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AccI GT'mk_AC
Cuts at: 560 1618 560
Size: 1058 3522
Acc65I G'GTAC_C
Cuts at: 1596 1596
Size: 4580
AclI AA'CG_TT
Cuts at: 4420 4420
Size: 4580
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 124 1328 2405 2837 3584 3852 124
Size: 1204 1077 432 747 268 852
Fragments arranged by size:
1204 1077 852 747 432 268
AflIII A'CryG_T
Cuts at: 206 1932 3036 4013 206
Size: 1726 1104 977 773
Fragments arranged by size:
1726 1104 977 773
AgeI A'CCGG_T
Cuts at: 2968 2968
Size: 4580
AlwNI CAG_nnn'CTG
Cuts at: 249 1276 1885 3452 249
Size: 1027 609 1567 1377
Fragments arranged by size:
1567 1377 1027 609
ApaLI G'TGCA_C
Cuts at: 3350 3350
Size: 4580
ApoI r'AATT_y
Cuts at: 505 1015 1041 1248 1434 1584 3933 4189 505
Size: 510 26 207 186 150 2349 256 896
Fragments arranged by size:
2349 896 510 256 207 186 150 26
AseI AT'TA_AT
Cuts at: 20 941 1511 20
Size: 921 570 3089
Fragments arranged by size:
3089 921 570
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4580
AvaI C'yCGr_G
Cuts at: 1600 1600
Size: 4580
AvrII C'CTAG_G
Cuts at: 1333 1333
Size: 4580
BamHI G'GATC_C
Cuts at: 1605 1605
Size: 4580
BanI G'GyrC_C
Cuts at: 1529 1596 2010 2258 2293 1529
Size: 67 414 248 35 3816
Fragments arranged by size:
3816 414 248 67 35
BanII G_rGCy'C
Cuts at: 1594 2624 1594
Size: 1030 3550
BbeI G_GCGC'C
Cuts at: 2014 2262 2014
Size: 248 4332
BciVI GTATCCnnnnn_n'
Cuts at: 2478 3239 4580 2478
Size: 761 1341 2478
Fragments arranged by size:
2478 1341 761
BclI T'GATC_A
Cuts at: 923 2100 923
Size: 1177 3403
BfrBI ATG'CAT
Cuts at: 522 522
Size: 4580
BglII A'GATC_T
Cuts at: 954 2095 4132 954
Size: 1141 2037 1402
Fragments arranged by size:
2037 1402 1141
BlpI GC'TnA_GC
Cuts at: 704 1692 704
Size: 988 3592
Bme1580I G_kGCm'C
Cuts at: 2205 2298 3354 2205
Size: 93 1056 3431
Fragments arranged by size:
3431 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1471 1792 1873 2208 4352 1471
Size: 321 81 335 2144 1699
Fragments arranged by size:
2144 1699 335 321 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 777 2947 777
Size: 2170 2410
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 563 1519 310
Size: 253 956 3371
Fragments arranged by size:
3371 956 253
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1756 2635 3127 3425 1756
Size: 879 492 298 2911
Fragments arranged by size:
2911 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 421 1059 4284 421
Size: 638 3225 717
Fragments arranged by size:
3225 717 638
BsaAI yAC'GTr
Cuts at: 1933 1933
Size: 4580
BsaBI GATnn'nnATC
Cuts at: 6 590 2094 4131 6
Size: 584 1504 2037 455
Fragments arranged by size:
2037 1504 584 455
BsaHI Gr'CG_yC
Cuts at: 2011 2259 2961 4457 2011
Size: 248 702 1496 2134
Fragments arranged by size:
2134 1496 702 248
BsaWI w'CCGG_w
Cuts at: 234 1769 2290 2968 3242 3389 3800 234
Size: 1535 521 678 274 147 411 1014
Fragments arranged by size:
1535 1014 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 178 208 178
Size: 30 4550
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 160 160
Size: 4580
BseYI C'CCAG_C
Cuts at: 334 848 2005 2763 3340 3836 334
Size: 514 1157 758 577 496 1078
Fragments arranged by size:
1157 1078 758 577 514 496
BsiEI CG_ry'CG
Cuts at: 104 2122 2168 3376 104
Size: 2018 46 1208 1308
Fragments arranged by size:
2018 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 710 1520 1594 2372 2562 3354 710
Size: 810 74 778 190 792 1936
Fragments arranged by size:
1936 810 792 778 190 74
BsmBI CGTCTCn'nnnn_
Cuts at: 385 385
Size: 4580
Bsp1286I G_dGCh'C
Cuts at: 710 1520 1594 2205 2298 2372 2562 2624 3354
Size: 810 74 611 93 74 190 62 730
Cuts at: 3354 710
Size: 1936
Fragments arranged by size:
1936 810 730 611 190 93 74 74
62
BspEI T'CCGG_A
Cuts at: 3800 3800
Size: 4580
BspHI T'CATG_A
Cuts at: 687 3756 687
Size: 3069 1511
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1630 1844 2146 2527 1630
Size: 214 302 381 3683
Fragments arranged by size:
3683 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2872 2926 4410 4575 50
Size: 2822 54 1484 165 55
Fragments arranged by size:
2822 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 621 2492 621
Size: 1871 2709
BsrFI r'CCGG_y
Cuts at: 2578 2759 2968 2578
Size: 181 209 4190
Fragments arranged by size:
4190 209 181
BssSI C'ACGA_G
Cuts at: 2851 3209 2851
Size: 358 4222
BstAPI GCAn_nnn'nTGC
Cuts at: 1957 1957
Size: 4580
BstBI TT'CG_AA
Cuts at: 2941 2941
Size: 4580
BstYI r'GATC_y
Cuts at: 1 417 954 1150 1605 2072 2095 2430 2676
Size: 416 537 196 455 467 23 335 246
Cuts at: 2676 3688 3774 3786 4132 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 537 467 455 449 416 346 335
246 196 86 23 12
BstZ17I GTA'TAC
Cuts at: 561 561
Size: 4580
BtgI C'CryG_G
Cuts at: 107 107
Size: 4580
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 508 2530 2711 2874 508
Size: 2022 181 163 2214
Fragments arranged by size:
2214 2022 181 163
BtsI GCAGTG_nn'
Cuts at: 130 1401 1823 130
Size: 1271 422 2887
Fragments arranged by size:
2887 1271 422
DraI TTT'AAA
Cuts at: 962 1579 962
Size: 617 3963
DrdI GACnn_nn'nnGTC
Cuts at: 2286 3144 2286
Size: 858 3722
EaeI y'GGCC_r
Cuts at: 809 2165 2339 2730 2757 4102 809
Size: 1356 174 391 27 1345 1287
Fragments arranged by size:
1356 1345 1287 391 174 27
EagI C'GGCC_G
Cuts at: 2165 2165
Size: 4580
EarI CTCTTCn'nnn_
Cuts at: 1206 2603 2813 1206
Size: 1397 210 2973
Fragments arranged by size:
2973 1397 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 181 2788 3098 3244 4390 181
Size: 2607 310 146 1146 371
Fragments arranged by size:
2607 1146 371 310 146
EcoICRI GAG'CTC
Cuts at: 1592 1592
Size: 4580
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 124 310 563 1328 1519 2405 2837 3584 3852
Size: 186 253 765 191 886 432 747 268
Cuts at: 3852 124
Size: 852
Fragments arranged by size:
886 852 765 747 432 268 253 191
186
EcoO109I rG'GnC_Cy
Cuts at: 1719 1719
Size: 4580
EcoRI G'AATT_C
Cuts at: 1434 1584 1434
Size: 150 4430
EcoRV GAT'ATC
Cuts at: 1238 1238
Size: 4580
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1268 1588 2599 1268
Size: 320 1011 3249
Fragments arranged by size:
3249 1011 320
FspI TGC'GCA
Cuts at: 2361 2361
Size: 4580
HaeII r_GCGC'y
Cuts at: 612 2014 2262 3284 4263 612
Size: 1402 248 1022 979 929
Fragments arranged by size:
1402 1022 979 929 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 178 210 2422 2452 2454 2484 178
Size: 32 2212 30 2 30 2274
Fragments arranged by size:
2274 2212 32 30 30 2
HincII GTy'rAC
Cuts at: 1619 1619
Size: 4580
KasI G'GCGC_C
Cuts at: 2010 2258 2010
Size: 248 4332
KpnI G_GTAC'C
Cuts at: 1600 1600
Size: 4580
MluI A'CGCG_T
Cuts at: 206 4013 206
Size: 3807 773
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3251 3435 3251
Size: 184 4396
MscI TGG'CCA
Cuts at: 2341 2341
Size: 4580
MslI CAynn'nnrTG
Cuts at: 1082 2569 2696 1082
Size: 1487 127 2966
Fragments arranged by size:
2966 1487 127
MspA1I CmG'CkG
Cuts at: 288 1691 2005 2365 3378 3623 288
Size: 1403 314 360 1013 245 1245
Fragments arranged by size:
1403 1245 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2761 2761
Size: 4580
NarI GG'CG_CC
Cuts at: 2011 2259 2011
Size: 248 4332
NcoI C'CATG_G
Cuts at: 107 107
Size: 4580
NdeI CA'TA_TG
Cuts at: 518 518
Size: 4580
NgoMIV G'CCGG_C
Cuts at: 2759 2759
Size: 4580
NsiI A_TGCA'T
Cuts at: 524 524
Size: 4580
NspI r_CATG'y
Cuts at: 454 937 1633 2133 3040 454
Size: 483 696 500 907 1994
Fragments arranged by size:
1994 907 696 500 483
PciI A'CATG_T
Cuts at: 3036 3036
Size: 4580
PmeI GTTT'AAAC
Cuts at: 1579 1579
Size: 4580
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3719 3751 3719
Size: 32 4548
PstI C_TGCA'G
Cuts at: 1627 2312 1627
Size: 685 3895
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4580
PvuII CAG'CTG
Cuts at: 288 2005 2365 288
Size: 1717 360 2503
Fragments arranged by size:
2503 1717 360
RsrII CG'GwC_CG
Cuts at: 2775 2775
Size: 4580
SacI G_AGCT'C
Cuts at: 1594 1594
Size: 4580
SalI G'TCGA_C
Cuts at: 1617 1617
Size: 4580
SapI GCTCTTCn'nnn_
Cuts at: 2603 2813 2603
Size: 210 4370
SbfI CC_TGCA'GG
Cuts at: 1627 1627
Size: 4580
ScaI AGT'ACT
Cuts at: 4097 4097
Size: 4580
SfcI C'TryA_G
Cuts at: 33 1623 2308 3301 3492 33
Size: 1590 685 993 191 1121
Fragments arranged by size:
1590 1121 993 685 191
SfoI GGC'GCC
Cuts at: 2012 2260 2012
Size: 248 4332
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4580
SmaI CCC'GGG
Cuts at: 1602 1602
Size: 4580
SmlI C'TyrA_G
Cuts at: 1735 2650 3142 3404 1735
Size: 915 492 262 2911
Fragments arranged by size:
2911 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4580
SphI G_CATG'C
Cuts at: 1633 2133 1633
Size: 500 4080
StyI C'CwwG_G
Cuts at: 107 1156 1333 1714 2068 107
Size: 1049 177 381 354 2619
Fragments arranged by size:
2619 1049 381 354 177
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 500 2163 2296 2755 2964 500
Size: 1663 133 459 209 2116
Fragments arranged by size:
2116 1663 459 209 133
TatI w'GTAC_w
Cuts at: 1417 4095 1417
Size: 2678 1902
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 196 228 4097 4129 196
Size: 32 3869 32 647
Fragments arranged by size:
3869 647 32 32
Tth111I GACn'n_nGTC
Cuts at: 2377 2377
Size: 4580
XbaI T'CTAG_A
Cuts at: 1611 1611
Size: 4580
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 718 1297 2075 718
Size: 579 778 3223
Fragments arranged by size:
3223 778 579
XmaI C'CCGG_G
Cuts at: 1600 1600
Size: 4580
XmnI GAAnn'nnTTC
Cuts at: 369 1221 1251 369
Size: 852 30 3698
Fragments arranged by size:
3698 852 30
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BstZ17I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NsiI NspI PciI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI BaeI BbsI BbvCI BcgI BglI BmgBI
BmtI Bpu10I BsgI BsiWI BsmI BsrGI BssHII BstEII
BstXI Bsu36I ClaI CspCI DraIII EcoNI FseI FspAI
HindIII HpaI MfeI NheI NotI NruI PacI PasI
PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI ZraI