Restriction map for
pF1KE0307
(Circular) (Six-base) MAPSORT of: pF1KE0307.seq Check: 3189 from: 1 to: 5165
pF1KE0307 5165 bp
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AccI GT'mk_AC
Cuts at: 2156 2203 2156
Size: 47 5118
Acc65I G'GTAC_C
Cuts at: 2181 2181
Size: 5165
AclI AA'CG_TT
Cuts at: 5005 5005
Size: 5165
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 296 446 790 811 1123 1174 1817 2990 3422
Size: 150 344 21 312 51 643 1173 432
Cuts at: 3422 4169 4437 296
Size: 747 268 1024
Fragments arranged by size:
1173 1024 747 643 432 344 312 268
150 51 21
AflIII A'CryG_T
Cuts at: 2517 3621 4598 2517
Size: 1104 977 3084
Fragments arranged by size:
3084 1104 977
AgeI A'CCGG_T
Cuts at: 3553 3553
Size: 5165
AlwNI CAG_nnn'CTG
Cuts at: 1032 2470 4037 1032
Size: 1438 1567 2160
Fragments arranged by size:
2160 1567 1438
ApaI G_GGCC'C
Cuts at: 2117 2117
Size: 5165
ApaLI G'TGCA_C
Cuts at: 259 3935 259
Size: 3676 1489
ApoI r'AATT_y
Cuts at: 683 1213 2169 4518 4774 683
Size: 530 956 2349 256 1074
Fragments arranged by size:
2349 1074 956 530 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5165
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5165
AvaI C'yCGr_G
Cuts at: 2185 2185
Size: 5165
BamHI G'GATC_C
Cuts at: 2190 2190
Size: 5165
BanI G'GyrC_C
Cuts at: 2040 2181 2595 2843 2878 2040
Size: 141 414 248 35 4327
Fragments arranged by size:
4327 414 248 141 35
BanII G_rGCy'C
Cuts at: 352 482 980 2057 2117 2179 3209 352
Size: 130 498 1077 60 62 1030 2308
Fragments arranged by size:
2308 1077 1030 498 130 62 60
BbeI G_GCGC'C
Cuts at: 2599 2847 2599
Size: 248 4917
BbsI GAAGACnn'nnnn_
Cuts at: 305 305
Size: 5165
BbvCI CC'TCA_GC
Cuts at: 2106 2106
Size: 5165
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1231 1265 1231
Size: 34 5131
BciVI GTATCCnnnnn_n'
Cuts at: 3063 3824 5165 3063
Size: 761 1341 3063
Fragments arranged by size:
3063 1341 761
BclI T'GATC_A
Cuts at: 1905 2685 1905
Size: 780 4385
BglI GCCn_nnn'nGGC
Cuts at: 112 1954 2061 112
Size: 1842 107 3216
Fragments arranged by size:
3216 1842 107
BglII A'GATC_T
Cuts at: 2680 4717 2680
Size: 2037 3128
BlpI GC'TnA_GC
Cuts at: 1181 2277 1181
Size: 1096 4069
Bme1580I G_kGCm'C
Cuts at: 263 816 2117 2790 2883 3939 263
Size: 553 1301 673 93 1056 1489
Fragments arranged by size:
1489 1301 1056 673 553 93
BmrI ACTGGGnnnn_n'
Cuts at: 176 223 2051 2377 2458 2793 4937 176
Size: 47 1828 326 81 335 2144 404
Fragments arranged by size:
2144 1828 404 335 326 81 47
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 748 3532 748
Size: 2784 2381
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 143 217 476 1988 143
Size: 74 259 1512 3320
Fragments arranged by size:
3320 1512 259 74
Bpu10I CC'TnA_GC
Cuts at: 2106 2106
Size: 5165
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1069 1705 2341 3220 3712 4010 1069
Size: 636 636 879 492 298 2224
Fragments arranged by size:
2224 879 636 636 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1888 4869 1888
Size: 2981 2184
BsaAI yAC'GTr
Cuts at: 817 2518 817
Size: 1701 3464
BsaBI GATnn'nnATC
Cuts at: 6 632 1414 2679 4716 6
Size: 626 782 1265 2037 455
Fragments arranged by size:
2037 1265 782 626 455
BsaHI Gr'CG_yC
Cuts at: 1712 1912 2153 2596 2844 3546 5042 1712
Size: 200 241 443 248 702 1496 1835
Fragments arranged by size:
1835 1496 702 443 248 241 200
BsaWI w'CCGG_w
Cuts at: 579 1939 2354 2875 3553 3827 3974 4385 579
Size: 1360 415 521 678 274 147 411 1359
Fragments arranged by size:
1360 1359 678 521 415 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 742 772 742
Size: 30 5135
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 652 1000 1096 1594 1778 2101 652
Size: 348 96 498 184 323 3716
Fragments arranged by size:
3716 498 348 323 184 96
BseYI C'CCAG_C
Cuts at: 2110 2117 2590 3348 3925 4421 2110
Size: 7 473 758 577 496 2854
Fragments arranged by size:
2854 758 577 496 473 7
BsiEI CG_ry'CG
Cuts at: 104 2707 2753 3961 104
Size: 2603 46 1208 1308
Fragments arranged by size:
2603 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 263 980 1225 1899 2027 2179 2957 3147 3939
Size: 717 245 674 128 152 778 190 792
Cuts at: 3939 263
Size: 1489
Fragments arranged by size:
1489 792 778 717 674 245 190 152
128
BsmI GAATG_Cn'
Cuts at: 384 384
Size: 5165
BsmBI CGTCTCn'nnnn_
Cuts at: 1478 1557 1478
Size: 79 5086
Bsp1286I G_dGCh'C
Cuts at: 263 352 482 816 980 1225 1899 2027 2057
Size: 89 130 334 164 245 674 128 30
Cuts at: 2057 2117 2179 2790 2883 2957 3147 3209 3939
Size: 60 62 611 93 74 190 62 730
Cuts at: 3939 263
Size: 1489
Fragments arranged by size:
1489 730 674 611 334 245 190 164
130 128 93 89 74 62 62 60
30
BspEI T'CCGG_A
Cuts at: 4385 4385
Size: 5165
BspHI T'CATG_A
Cuts at: 1628 4341 1628
Size: 2713 2452
BspMI ACCTGCnnnn'nnnn_
Cuts at: 554 2215 2429 2731 3112 554
Size: 1661 214 302 381 2607
Fragments arranged by size:
2607 1661 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1717 3457 3511 4995 5160 50
Size: 1667 1740 54 1484 165 55
Fragments arranged by size:
1740 1667 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3077 3077
Size: 5165
BsrFI r'CCGG_y
Cuts at: 3163 3344 3553 3163
Size: 181 209 4775
Fragments arranged by size:
4775 209 181
BssSI C'ACGA_G
Cuts at: 2072 3436 3794 2072
Size: 1364 358 3443
Fragments arranged by size:
3443 1364 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2542 2542
Size: 5165
BstBI TT'CG_AA
Cuts at: 3526 3526
Size: 5165
BstYI r'GATC_y
Cuts at: 1 2190 2657 2680 3015 3261 4273 4359 4371
Size: 2189 467 23 335 246 1012 86 12
Cuts at: 4371 4717 1
Size: 346 449
Fragments arranged by size:
2189 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1790 1790
Size: 5165
BtgI C'CryG_G
Cuts at: 107 2036 107
Size: 1929 3236
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3115 3296 3459 3115
Size: 181 163 4821
Fragments arranged by size:
4821 181 163
BtsI GCAGTG_nn'
Cuts at: 1818 2408 1818
Size: 590 4575
ClaI AT'CG_AT
Cuts at: 965 965
Size: 5165
DraI TTT'AAA
Cuts at: 1008 2164 1008
Size: 1156 4009
DrdI GACnn_nn'nnGTC
Cuts at: 2871 3729 2871
Size: 858 4307
EaeI y'GGCC_r
Cuts at: 210 1616 2750 2924 3315 3342 4687 210
Size: 1406 1134 174 391 27 1345 688
Fragments arranged by size:
1406 1345 1134 688 391 174 27
EagI C'GGCC_G
Cuts at: 2750 2750
Size: 5165
EarI CTCTTCn'nnn_
Cuts at: 272 422 432 791 821 1364 1448 1529 2015
Size: 150 10 359 30 543 84 81 486
Cuts at: 2015 3188 3398 272
Size: 1173 210 2039
Fragments arranged by size:
2039 1173 543 486 359 210 150 84
81 30 10
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3373 3683 3829 4975 3373
Size: 310 146 1146 3563
Fragments arranged by size:
3563 1146 310 146
EcoICRI GAG'CTC
Cuts at: 978 2177 978
Size: 1199 3966
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 143 217 296 446 476 790 811 1123 1174
Size: 74 79 150 30 314 21 312 51
Cuts at: 1174 1817 1988 2990 3422 4169 4437 143
Size: 643 171 1002 432 747 268 871
Fragments arranged by size:
1002 871 747 643 432 314 312 268
171 150 79 74 51 30 21
EcoNI CCTnn'n_nnAGG
Cuts at: 199 199
Size: 5165
EcoO109I rG'GnC_Cy
Cuts at: 1947 2102 2114 2304 1947
Size: 155 12 190 4808
Fragments arranged by size:
4808 190 155 12
EcoRI G'AATT_C
Cuts at: 1213 2169 1213
Size: 956 4209
EcoRV GAT'ATC
Cuts at: 270 270
Size: 5165
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 820 3184 820
Size: 2364 2801
FspI TGC'GCA
Cuts at: 2013 2946 2013
Size: 933 4232
HaeII r_GCGC'y
Cuts at: 805 2599 2847 3869 4848 805
Size: 1794 248 1022 979 1122
Fragments arranged by size:
1794 1122 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 157 189 1528 1560 1623 1655 1897 1929 3007
Size: 32 1339 32 63 32 242 32 1078
Cuts at: 3007 3037 3039 3069 157
Size: 30 2 30 2253
Fragments arranged by size:
2253 1339 1078 242 63 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 152 1652 2157 2204 152
Size: 1500 505 47 3113
Fragments arranged by size:
3113 1500 505 47
HindIII A'AGCT_T
Cuts at: 1045 1934 1045
Size: 889 4276
KasI G'GCGC_C
Cuts at: 2595 2843 2595
Size: 248 4917
KpnI G_GTAC'C
Cuts at: 2185 2185
Size: 5165
MluI A'CGCG_T
Cuts at: 4598 4598
Size: 5165
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 247 258 1337 1872 3836 4020 247
Size: 11 1079 535 1964 184 1392
Fragments arranged by size:
1964 1392 1079 535 184 11
MscI TGG'CCA
Cuts at: 212 1618 2926 212
Size: 1406 1308 2451
Fragments arranged by size:
2451 1406 1308
MslI CAynn'nnrTG
Cuts at: 3154 3281 3154
Size: 127 5038
MspA1I CmG'CkG
Cuts at: 139 294 810 2110 2276 2590 2950 3963 4208
Size: 155 516 1300 166 314 360 1013 245
Cuts at: 4208 139
Size: 1096
Fragments arranged by size:
1300 1096 1013 516 360 314 245 166
155
NaeI GCC'GGC
Cuts at: 3346 3346
Size: 5165
NarI GG'CG_CC
Cuts at: 2596 2844 2596
Size: 248 4917
NcoI C'CATG_G
Cuts at: 107 2036 107
Size: 1929 3236
NgoMIV G'CCGG_C
Cuts at: 3344 3344
Size: 5165
NspI r_CATG'y
Cuts at: 2012 2051 2218 2718 3625 2012
Size: 39 167 500 907 3552
Fragments arranged by size:
3552 907 500 167 39
PciI A'CATG_T
Cuts at: 3621 3621
Size: 5165
PfoI T'CCnGG_A
Cuts at: 1915 1915
Size: 5165
PmeI GTTT'AAAC
Cuts at: 2164 2164
Size: 5165
PmlI CAC'GTG
Cuts at: 817 817
Size: 5165
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4304 4336 4304
Size: 32 5133
PshAI GACnn'nnGTC
Cuts at: 1514 1514
Size: 5165
PspOMI G'GGCC_C
Cuts at: 2113 2113
Size: 5165
PstI C_TGCA'G
Cuts at: 773 1025 1178 2212 2897 773
Size: 252 153 1034 685 3041
Fragments arranged by size:
3041 1034 685 252 153
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5165
PvuII CAG'CTG
Cuts at: 294 2110 2590 2950 294
Size: 1816 480 360 2509
Fragments arranged by size:
2509 1816 480 360
RsrII CG'GwC_CG
Cuts at: 3360 3360
Size: 5165
SacI G_AGCT'C
Cuts at: 980 2179 980
Size: 1199 3966
SalI G'TCGA_C
Cuts at: 2155 2202 2155
Size: 47 5118
SapI GCTCTTCn'nnn_
Cuts at: 272 1529 2015 3188 3398 272
Size: 1257 486 1173 210 2039
Fragments arranged by size:
2039 1257 1173 486 210
SbfI CC_TGCA'GG
Cuts at: 1178 2212 1178
Size: 1034 4131
ScaI AGT'ACT
Cuts at: 4682 4682
Size: 5165
SfcI C'TryA_G
Cuts at: 33 769 1021 1174 1975 2208 2893 3886 4077
Size: 736 252 153 801 233 685 993 191
Cuts at: 4077 33
Size: 1121
Fragments arranged by size:
1121 993 801 736 685 252 233 191
153
SfoI GGC'GCC
Cuts at: 2597 2845 2597
Size: 248 4917
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5165
SmaI CCC'GGG
Cuts at: 2187 2187
Size: 5165
SmlI C'TyrA_G
Cuts at: 1048 1720 2320 3235 3727 3989 1048
Size: 672 600 915 492 262 2224
Fragments arranged by size:
2224 915 672 600 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5165
SphI G_CATG'C
Cuts at: 2012 2051 2218 2718 2012
Size: 39 167 500 4459
Fragments arranged by size:
4459 500 167 39
SspI AAT'ATT
Cuts at: 1004 1108 1004
Size: 104 5061
StuI AGG'CCT
Cuts at: 1969 1969
Size: 5165
StyI C'CwwG_G
Cuts at: 107 1832 2036 2299 2653 107
Size: 1725 204 263 354 2619
Fragments arranged by size:
2619 1725 354 263 204
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 480 2748 2881 3340 3549 480
Size: 2268 133 459 209 2096
Fragments arranged by size:
2268 2096 459 209 133
TatI w'GTAC_w
Cuts at: 4680 4680
Size: 5165
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 742 774 4682 4714 742
Size: 32 3908 32 1193
Fragments arranged by size:
3908 1193 32 32
Tth111I GACn'n_nGTC
Cuts at: 2962 2962
Size: 5165
XbaI T'CTAG_A
Cuts at: 2196 2196
Size: 5165
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2660 2660
Size: 5165
XmaI C'CCGG_G
Cuts at: 2185 2185
Size: 5165
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI ClaI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PfoI PmeI PmlI PpiI PshAI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BfrBI BmgBI BmtI BsgI BsiWI
BsrGI BssHII BstEII BstXI BstZ17I CspCI DraIII FseI
FspAI HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PpuMI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI
XmnI ZraI