Restriction map for
pF1KE0309
(Circular) (Six-base) MAPSORT of: pF1KE0309.seq Check: 6184 from: 1 to: 4835
pF1KE0309 4835 bp
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 932 994 932
Size: 62 4773
AccI GT'mk_AC
Cuts at: 878 1873 878
Size: 995 3840
Acc65I G'GTAC_C
Cuts at: 1851 1851
Size: 4835
AclI AA'CG_TT
Cuts at: 4675 4675
Size: 4835
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1422 2660 3092 3839 4107 1422
Size: 1238 432 747 268 2150
Fragments arranged by size:
2150 1238 747 432 268
AfeI AGC'GCT
Cuts at: 1507 1507
Size: 4835
AflIII A'CryG_T
Cuts at: 2187 3291 4268 2187
Size: 1104 977 2754
Fragments arranged by size:
2754 1104 977
AgeI A'CCGG_T
Cuts at: 3223 3223
Size: 4835
AhdI GACnn_n'nnGTC
Cuts at: 1710 1710
Size: 4835
AlwNI CAG_nnn'CTG
Cuts at: 2140 3707 2140
Size: 1567 3268
ApaLI G'TGCA_C
Cuts at: 145 3605 145
Size: 3460 1375
ApoI r'AATT_y
Cuts at: 250 1839 4188 4444 250
Size: 1589 2349 256 641
Fragments arranged by size:
2349 1589 641 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4835
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4835
AvaI C'yCGr_G
Cuts at: 674 1855 674
Size: 1181 3654
BamHI G'GATC_C
Cuts at: 1860 1860
Size: 4835
BanI G'GyrC_C
Cuts at: 1478 1619 1679 1851 2265 2513 2548 1478
Size: 141 60 172 414 248 35 3765
Fragments arranged by size:
3765 414 248 172 141 60 35
BanII G_rGCy'C
Cuts at: 1360 1849 2879 1360
Size: 489 1030 3316
Fragments arranged by size:
3316 1030 489
BbeI G_GCGC'C
Cuts at: 1683 2269 2517 1683
Size: 586 248 4001
Fragments arranged by size:
4001 586 248
BbsI GAAGACnn'nnnn_
Cuts at: 659 659
Size: 4835
BbvCI CC'TCA_GC
Cuts at: 1297 1579 1297
Size: 282 4553
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 441 475 945 979 1024 1058 441
Size: 34 470 34 45 34 4218
Fragments arranged by size:
4218 470 45 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2733 3494 4835 2733
Size: 761 1341 2733
Fragments arranged by size:
2733 1341 761
BclI T'GATC_A
Cuts at: 383 2355 383
Size: 1972 2863
BglI GCCn_nnn'nGGC
Cuts at: 376 1094 1777 376
Size: 718 683 3434
Fragments arranged by size:
3434 718 683
BglII A'GATC_T
Cuts at: 1259 1433 2350 4387 1259
Size: 174 917 2037 1707
Fragments arranged by size:
2037 1707 917 174
BlpI GC'TnA_GC
Cuts at: 1156 1947 1156
Size: 791 4044
Bme1580I G_kGCm'C
Cuts at: 149 261 372 2460 2553 3609 149
Size: 112 111 2088 93 1056 1375
Fragments arranged by size:
2088 1375 1056 112 111 93
BmgBI CAC'GTC
Cuts at: 225 657 225
Size: 432 4403
BmrI ACTGGGnnnn_n'
Cuts at: 804 2047 2128 2463 4607 804
Size: 1243 81 335 2144 1032
Fragments arranged by size:
2144 1243 1032 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1332 3202 1332
Size: 1870 2965
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 394 154
Size: 240 4595
Bpu10I CC'TnA_GC
Cuts at: 770 1297 1579 1720 770
Size: 527 282 141 3885
Fragments arranged by size:
3885 527 282 141
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 217 682 2011 2890 3382 3680 217
Size: 465 1329 879 492 298 1372
Fragments arranged by size:
1372 1329 879 492 465 298
BsaI GGTCTCn'nnnn_
Cuts at: 1303 1662 4539 1303
Size: 359 2877 1599
Fragments arranged by size:
2877 1599 359
BsaAI yAC'GTr
Cuts at: 2188 2188
Size: 4835
BsaBI GATnn'nnATC
Cuts at: 6 2349 4386 6
Size: 2343 2037 455
Fragments arranged by size:
2343 2037 455
BsaHI Gr'CG_yC
Cuts at: 185 482 1680 2266 2514 3216 4712 185
Size: 297 1198 586 248 702 1496 308
Fragments arranged by size:
1496 1198 702 586 308 297 248
BsaWI w'CCGG_w
Cuts at: 263 2024 2545 3223 3497 3644 4055 263
Size: 1761 521 678 274 147 411 1043
Fragments arranged by size:
1761 1043 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 142 208 715 1810 142
Size: 66 507 1095 3167
Fragments arranged by size:
3167 1095 507 66
BseYI C'CCAG_C
Cuts at: 1052 1375 2260 3018 3595 4091 1052
Size: 323 885 758 577 496 1796
Fragments arranged by size:
1796 885 758 577 496 323
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 474 939 474
Size: 465 4370
BsiEI CG_ry'CG
Cuts at: 104 744 2377 2423 3631 104
Size: 640 1633 46 1208 1308
Fragments arranged by size:
1633 1308 1208 640 46
BsiHKAI G_wGCw'C
Cuts at: 149 1162 1360 1849 2627 2817 3609 149
Size: 1013 198 489 778 190 792 1375
Fragments arranged by size:
1375 1013 792 778 489 198 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1318 1318
Size: 4835
Bsp1286I G_dGCh'C
Cuts at: 149 261 372 1162 1360 1849 2460 2553 2627
Size: 112 111 790 198 489 611 93 74
Cuts at: 2627 2817 2879 3609 149
Size: 190 62 730 1375
Fragments arranged by size:
1375 790 730 611 489 198 190 112
111 93 74 62
BspEI T'CCGG_A
Cuts at: 4055 4055
Size: 4835
BspHI T'CATG_A
Cuts at: 4011 4011
Size: 4835
BspMI ACCTGCnnnn'nnnn_
Cuts at: 696 773 932 994 1885 2099 2401 2782 696
Size: 77 159 62 891 214 302 381 2749
Fragments arranged by size:
2749 891 381 302 214 159 77 62
BsrBI CCG'CTC
Cuts at: 50 3127 3181 4665 4830 50
Size: 3077 54 1484 165 55
Fragments arranged by size:
3077 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 436 1040 1583 2747 436
Size: 604 543 1164 2524
Fragments arranged by size:
2524 1164 604 543
BsrFI r'CCGG_y
Cuts at: 914 1511 2833 3014 3223 914
Size: 597 1322 181 209 2526
Fragments arranged by size:
2526 1322 597 209 181
BsrGI T'GTAC_A
Cuts at: 1595 1595
Size: 4835
BssSI C'ACGA_G
Cuts at: 3106 3464 3106
Size: 358 4477
BstAPI GCAn_nnn'nTGC
Cuts at: 218 2212 218
Size: 1994 2841
BstBI TT'CG_AA
Cuts at: 3196 3196
Size: 4835
BstEII G'GTnAC_C
Cuts at: 1344 1344
Size: 4835
BstYI r'GATC_y
Cuts at: 1 138 197 1259 1433 1860 2327 2350 2685
Size: 137 59 1062 174 427 467 23 335
Cuts at: 2685 2931 3943 4029 4041 4387 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1062 1012 467 449 427 346 335 246
174 137 86 59 23 12
BtgI C'CryG_G
Cuts at: 107 1232 1304 1775 107
Size: 1125 72 471 3167
Fragments arranged by size:
3167 1125 471 72
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 948 2785 2966 3129 948
Size: 1837 181 163 2654
Fragments arranged by size:
2654 1837 181 163
BtsI GCAGTG_nn'
Cuts at: 1374 1449 2078 1374
Size: 75 629 4131
Fragments arranged by size:
4131 629 75
DraI TTT'AAA
Cuts at: 1834 1834
Size: 4835
DraIII CAC_nnn'GTG
Cuts at: 609 609
Size: 4835
DrdI GACnn_nn'nnGTC
Cuts at: 2541 3399 2541
Size: 858 3977
EaeI y'GGCC_r
Cuts at: 943 1143 1416 1667 2420 2594 2985 3012 4357
Size: 200 273 251 753 174 391 27 1345
Cuts at: 4357 943
Size: 1421
Fragments arranged by size:
1421 1345 753 391 273 251 200 174
27
EagI C'GGCC_G
Cuts at: 2420 2420
Size: 4835
EarI CTCTTCn'nnn_
Cuts at: 623 1296 2858 3068 623
Size: 673 1562 210 2390
Fragments arranged by size:
2390 1562 673 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 778 3043 3353 3499 4645 778
Size: 2265 310 146 1146 968
Fragments arranged by size:
2265 1146 968 310 146
EcoICRI GAG'CTC
Cuts at: 1358 1847 1358
Size: 489 4346
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 394 1422 2660 3092 3839 4107 154
Size: 240 1028 1238 432 747 268 882
Fragments arranged by size:
1238 1028 882 747 432 268 240
EcoO109I rG'GnC_Cy
Cuts at: 757 870 990 1974 757
Size: 113 120 984 3618
Fragments arranged by size:
3618 984 120 113
EcoRI G'AATT_C
Cuts at: 1839 1839
Size: 4835
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 676 2854 676
Size: 2178 2657
FspI TGC'GCA
Cuts at: 2616 2616
Size: 4835
HaeII r_GCGC'y
Cuts at: 915 1509 1683 2269 2517 3539 4518 915
Size: 594 174 586 248 1022 979 1232
Fragments arranged by size:
1232 1022 979 594 586 248 174
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 655 687 763 795 1050 1082 1273 1305 1347
Size: 32 76 32 255 32 191 32 42
Cuts at: 1347 1379 1696 1728 2677 2707 2709 2739 655
Size: 32 317 32 949 30 2 30 2751
Fragments arranged by size:
2751 949 317 255 191 76 42 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1874 1874
Size: 4835
KasI G'GCGC_C
Cuts at: 1679 2265 2513 1679
Size: 586 248 4001
Fragments arranged by size:
4001 586 248
KpnI G_GTAC'C
Cuts at: 1855 1855
Size: 4835
MfeI C'AATT_G
Cuts at: 1047 1047
Size: 4835
MluI A'CGCG_T
Cuts at: 4268 4268
Size: 4835
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3506 3690 3506
Size: 184 4651
MscI TGG'CCA
Cuts at: 1669 2596 1669
Size: 927 3908
MslI CAynn'nnrTG
Cuts at: 2824 2951 2824
Size: 127 4708
MspA1I CmG'CkG
Cuts at: 465 1489 1673 1946 2260 2620 3633 3878 465
Size: 1024 184 273 314 360 1013 245 1422
Fragments arranged by size:
1422 1024 1013 360 314 273 245 184
NaeI GCC'GGC
Cuts at: 916 1513 3016 916
Size: 597 1503 2735
Fragments arranged by size:
2735 1503 597
NarI GG'CG_CC
Cuts at: 1680 2266 2514 1680
Size: 586 248 4001
Fragments arranged by size:
4001 586 248
NcoI C'CATG_G
Cuts at: 107 1232 1775 107
Size: 1125 543 3167
Fragments arranged by size:
3167 1125 543
NgoMIV G'CCGG_C
Cuts at: 914 1511 3014 914
Size: 597 1503 2735
Fragments arranged by size:
2735 1503 597
NspI r_CATG'y
Cuts at: 1497 1888 2388 3295 1497
Size: 391 500 907 3037
Fragments arranged by size:
3037 907 500 391
PasI CC'CwG_GG
Cuts at: 1530 1530
Size: 4835
PciI A'CATG_T
Cuts at: 3291 3291
Size: 4835
PflMI CCAn_nnn'nTGG
Cuts at: 1052 1052
Size: 4835
PmeI GTTT'AAAC
Cuts at: 1834 1834
Size: 4835
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3974 4006 3974
Size: 32 4803
PpuMI rG'GwC_Cy
Cuts at: 757 870 757
Size: 113 4722
PstI C_TGCA'G
Cuts at: 464 470 1488 1882 2567 464
Size: 6 1018 394 685 2732
Fragments arranged by size:
2732 1018 685 394 6
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4835
PvuII CAG'CTG
Cuts at: 465 1489 1673 2260 2620 465
Size: 1024 184 587 360 2680
Fragments arranged by size:
2680 1024 587 360 184
RsrII CG'GwC_CG
Cuts at: 3030 3030
Size: 4835
SacI G_AGCT'C
Cuts at: 1360 1849 1360
Size: 489 4346
SalI G'TCGA_C
Cuts at: 1872 1872
Size: 4835
SanDI GG'GwC_CC
Cuts at: 757 757
Size: 4835
SapI GCTCTTCn'nnn_
Cuts at: 2858 3068 2858
Size: 210 4625
SbfI CC_TGCA'GG
Cuts at: 1882 1882
Size: 4835
ScaI AGT'ACT
Cuts at: 4352 4352
Size: 4835
SexAI A'CCwGG_T
Cuts at: 842 872 842
Size: 30 4805
SfcI C'TryA_G
Cuts at: 33 460 466 983 1484 1878 2563 3556 3747
Size: 427 6 517 501 394 685 993 191
Cuts at: 3747 33
Size: 1121
Fragments arranged by size:
1121 993 685 517 501 427 394 191
6
SfoI GGC'GCC
Cuts at: 1681 2267 2515 1681
Size: 586 248 4001
Fragments arranged by size:
4001 586 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4835
SmaI CCC'GGG
Cuts at: 1857 1857
Size: 4835
SmlI C'TyrA_G
Cuts at: 232 661 1990 2905 3397 3659 232
Size: 429 1329 915 492 262 1408
Fragments arranged by size:
1408 1329 915 492 429 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4835
SphI G_CATG'C
Cuts at: 1497 1888 2388 1497
Size: 391 500 3944
Fragments arranged by size:
3944 500 391
StuI AGG'CCT
Cuts at: 1171 1171
Size: 4835
StyI C'CwwG_G
Cuts at: 107 647 1232 1775 1969 2323 107
Size: 540 585 543 194 354 2619
Fragments arranged by size:
2619 585 543 540 354 194
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 520 530 2418 2551 3010 3219 520
Size: 10 1888 133 459 209 2136
Fragments arranged by size:
2136 1888 459 209 133 10
TatI w'GTAC_w
Cuts at: 828 1595 4350 828
Size: 767 2755 1313
Fragments arranged by size:
2755 1313 767
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4352 4384 4352
Size: 32 4803
Tth111I GACn'n_nGTC
Cuts at: 2632 2632
Size: 4835
XbaI T'CTAG_A
Cuts at: 1866 1866
Size: 4835
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2330 2330
Size: 4835
XmaI C'CCGG_G
Cuts at: 1855 1855
Size: 4835
XmnI GAAnn'nnTTC
Cuts at: 509 509
Size: 4835
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AleI AlfI AloI ApaI AscI AvrII
BaeI BfrBI BmtI BsaXI BsiWI BsmI BssHII BstXI
BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI
HindIII HpaI NdeI NheI NotI NruI NsiI PacI
PfoI PmlI PshAI PsiI PspOMI PspXI PsrI SacII
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI