Restriction map for
pF1KE0312
(Circular) (Six-base) MAPSORT of: pF1KE0312.seq Check: 9837 from: 1 to: 4688
pF1KE0312 4688 bp
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 418 1391 418
Size: 973 3715
AccI GT'mk_AC
Cuts at: 862 1726 862
Size: 864 3824
Acc65I G'GTAC_C
Cuts at: 1469 1704 1469
Size: 235 4453
AclI AA'CG_TT
Cuts at: 4528 4528
Size: 4688
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 661 1525 1643 2513 2945 3692 3960 661
Size: 864 118 870 432 747 268 1389
Fragments arranged by size:
1389 870 864 747 432 268 118
AfeI AGC'GCT
Cuts at: 1022 1022
Size: 4688
AflIII A'CryG_T
Cuts at: 2040 3144 4121 2040
Size: 1104 977 2607
Fragments arranged by size:
2607 1104 977
AgeI A'CCGG_T
Cuts at: 3076 3076
Size: 4688
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1256 1288 1256
Size: 32 4656
AlwNI CAG_nnn'CTG
Cuts at: 325 838 923 1392 1993 3560 325
Size: 513 85 469 601 1567 1453
Fragments arranged by size:
1567 1453 601 513 469 85
ApaI G_GGCC'C
Cuts at: 276 276
Size: 4688
ApaLI G'TGCA_C
Cuts at: 3458 3458
Size: 4688
ApoI r'AATT_y
Cuts at: 1692 4041 4297 1692
Size: 2349 256 2083
Fragments arranged by size:
2349 2083 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4688
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4688
AvaI C'yCGr_G
Cuts at: 380 471 1238 1708 380
Size: 91 767 470 3360
Fragments arranged by size:
3360 767 470 91
BamHI G'GATC_C
Cuts at: 1713 1713
Size: 4688
BanI G'GyrC_C
Cuts at: 1318 1413 1469 1704 2118 2366 2401 1318
Size: 95 56 235 414 248 35 3605
Fragments arranged by size:
3605 414 248 235 95 56 35
BanII G_rGCy'C
Cuts at: 276 568 1088 1535 1702 2732 276
Size: 292 520 447 167 1030 2232
Fragments arranged by size:
2232 1030 520 447 292 167
BbeI G_GCGC'C
Cuts at: 2122 2370 2122
Size: 248 4440
BbvCI CC'TCA_GC
Cuts at: 326 1379 326
Size: 1053 3635
BciVI GTATCCnnnnn_n'
Cuts at: 175 2586 3347 4688 175
Size: 2411 761 1341 175
Fragments arranged by size:
2411 1341 761 175
BclI T'GATC_A
Cuts at: 2208 2208
Size: 4688
BglI GCCn_nnn'nGGC
Cuts at: 1316 1497 1316
Size: 181 4507
BglII A'GATC_T
Cuts at: 2203 4240 2203
Size: 2037 2651
BlpI GC'TnA_GC
Cuts at: 1800 1800
Size: 4688
Bme1580I G_kGCm'C
Cuts at: 276 1321 2313 2406 3462 276
Size: 1045 992 93 1056 1502
Fragments arranged by size:
1502 1056 1045 992 93
BmgBI CAC'GTC
Cuts at: 1035 1035
Size: 4688
BmrI ACTGGGnnnn_n'
Cuts at: 1128 1194 1900 1981 2316 4460 1128
Size: 66 706 81 335 2144 1356
Fragments arranged by size:
2144 1356 706 335 81 66
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 990 3055 990
Size: 2065 2623
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 754 754
Size: 4688
Bpu10I CC'TnA_GC
Cuts at: 266 326 1379 1407 266
Size: 60 1053 28 3547
Fragments arranged by size:
3547 1053 60 28
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 428 1072 1864 2743 3235 3533 428
Size: 644 792 879 492 298 1583
Fragments arranged by size:
1583 879 792 644 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4392 4392
Size: 4688
BsaAI yAC'GTr
Cuts at: 2041 2041
Size: 4688
BsaBI GATnn'nnATC
Cuts at: 6 2202 4239 6
Size: 2196 2037 455
Fragments arranged by size:
2196 2037 455
BsaHI Gr'CG_yC
Cuts at: 2119 2367 3069 4565 2119
Size: 248 702 1496 2242
Fragments arranged by size:
2242 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1877 2398 3076 3350 3497 3908 1877
Size: 521 678 274 147 411 2657
Fragments arranged by size:
2657 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1256 1286 1256
Size: 30 4658
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 145 148 1087 1090 145
Size: 3 939 3 3743
Fragments arranged by size:
3743 939 3 3
BseYI C'CCAG_C
Cuts at: 318 758 1373 2113 2871 3448 3944 318
Size: 440 615 740 758 577 496 1062
Fragments arranged by size:
1062 758 740 615 577 496 440
BsiEI CG_ry'CG
Cuts at: 104 2230 2276 3484 104
Size: 2126 46 1208 1308
Fragments arranged by size:
2126 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1088 1408 1702 2480 2670 3462 1088
Size: 320 294 778 190 792 2314
Fragments arranged by size:
2314 792 778 320 294 190
Bsp1286I G_dGCh'C
Cuts at: 276 568 1088 1321 1408 1535 1702 2313 2406
Size: 292 520 233 87 127 167 611 93
Cuts at: 2406 2480 2670 2732 3462 276
Size: 74 190 62 730 1502
Fragments arranged by size:
1502 730 611 520 292 233 190 167
127 93 87 74 62
BspEI T'CCGG_A
Cuts at: 3908 3908
Size: 4688
BspHI T'CATG_A
Cuts at: 3864 3864
Size: 4688
BspMI ACCTGCnnnn'nnnn_
Cuts at: 418 552 1391 1738 1952 2254 2635 418
Size: 134 839 347 214 302 381 2471
Fragments arranged by size:
2471 839 381 347 302 214 134
BsrBI CCG'CTC
Cuts at: 50 161 270 289 1050 1365 2980 3034 4518
Size: 111 109 19 761 315 1615 54 1484
Cuts at: 4518 4683 50
Size: 165 55
Fragments arranged by size:
1615 1484 761 315 165 111 109 55
54 19
BsrDI GCAATG_nn'
Cuts at: 813 1672 2600 813
Size: 859 928 2901
Fragments arranged by size:
2901 928 859
BsrFI r'CCGG_y
Cuts at: 254 479 2686 2867 3076 254
Size: 225 2207 181 209 1866
Fragments arranged by size:
2207 1866 225 209 181
BssSI C'ACGA_G
Cuts at: 2959 3317 2959
Size: 358 4330
BstAPI GCAn_nnn'nTGC
Cuts at: 413 2065 413
Size: 1652 3036
BstBI TT'CG_AA
Cuts at: 3049 3049
Size: 4688
BstYI r'GATC_y
Cuts at: 1 1713 2180 2203 2538 2784 3796 3882 3894
Size: 1712 467 23 335 246 1012 86 12
Cuts at: 3894 4240 1
Size: 346 449
Fragments arranged by size:
1712 1012 467 449 346 335 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 863 863
Size: 4688
Bsu36I CC'TnA_GG
Cuts at: 935 1418 935
Size: 483 4205
BtgI C'CryG_G
Cuts at: 107 1473 107
Size: 1366 3322
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2638 2819 2982 2638
Size: 181 163 4344
Fragments arranged by size:
4344 181 163
BtsI GCAGTG_nn'
Cuts at: 1582 1931 1582
Size: 349 4339
DraI TTT'AAA
Cuts at: 1687 1687
Size: 4688
DrdI GACnn_nn'nnGTC
Cuts at: 2394 3252 2394
Size: 858 3830
EaeI y'GGCC_r
Cuts at: 353 1437 2273 2447 2838 2865 4210 353
Size: 1084 836 174 391 27 1345 831
Fragments arranged by size:
1345 1084 836 831 391 174 27
EagI C'GGCC_G
Cuts at: 2273 2273
Size: 4688
EarI CTCTTCn'nnn_
Cuts at: 215 845 950 959 1501 2711 2921 215
Size: 630 105 9 542 1210 210 1982
Fragments arranged by size:
1982 1210 630 542 210 105 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 142 1365 1579 2896 3206 3352 4498 142
Size: 1223 214 1317 310 146 1146 332
Fragments arranged by size:
1317 1223 1146 332 310 214 146
EcoICRI GAG'CTC
Cuts at: 1086 1700 1086
Size: 614 4074
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 661 754 1525 1643 2513 2945 3692 3960 661
Size: 93 771 118 870 432 747 268 1389
Fragments arranged by size:
1389 870 771 747 432 268 118 93
EcoNI CCTnn'n_nnAGG
Cuts at: 1638 1638
Size: 4688
EcoO109I rG'GnC_Cy
Cuts at: 273 1389 1422 1511 1827 273
Size: 1116 33 89 316 3134
Fragments arranged by size:
3134 1116 316 89 33
EcoRI G'AATT_C
Cuts at: 1692 1692
Size: 4688
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2707 2707
Size: 4688
FspI TGC'GCA
Cuts at: 2469 2469
Size: 4688
HaeII r_GCGC'y
Cuts at: 115 801 1024 1332 2122 2370 3392 4371 115
Size: 686 223 308 790 248 1022 979 432
Fragments arranged by size:
1022 979 790 686 432 308 248 223
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1232 1264 1654 1686 2530 2560 2562 2592 1232
Size: 32 390 32 844 30 2 30 3328
Fragments arranged by size:
3328 844 390 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1727 1727
Size: 4688
KasI G'GCGC_C
Cuts at: 2118 2366 2118
Size: 248 4440
KpnI G_GTAC'C
Cuts at: 1473 1708 1473
Size: 235 4453
MluI A'CGCG_T
Cuts at: 4121 4121
Size: 4688
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 583 3359 3543 583
Size: 2776 184 1728
Fragments arranged by size:
2776 1728 184
MscI TGG'CCA
Cuts at: 1439 2449 1439
Size: 1010 3678
MslI CAynn'nnrTG
Cuts at: 977 1201 1609 2677 2804 977
Size: 224 408 1068 127 2861
Fragments arranged by size:
2861 1068 408 224 127
MspA1I CmG'CkG
Cuts at: 325 762 1799 2113 2473 3486 3731 325
Size: 437 1037 314 360 1013 245 1282
Fragments arranged by size:
1282 1037 1013 437 360 314 245
NaeI GCC'GGC
Cuts at: 2869 2869
Size: 4688
NarI GG'CG_CC
Cuts at: 2119 2367 2119
Size: 248 4440
NcoI C'CATG_G
Cuts at: 107 1473 107
Size: 1366 3322
NgoMIV G'CCGG_C
Cuts at: 2867 2867
Size: 4688
NspI r_CATG'y
Cuts at: 468 1741 2241 3148 468
Size: 1273 500 907 2008
Fragments arranged by size:
2008 1273 907 500
PciI A'CATG_T
Cuts at: 3144 3144
Size: 4688
PflMI CCAn_nnn'nTGG
Cuts at: 1392 1392
Size: 4688
PmeI GTTT'AAAC
Cuts at: 1687 1687
Size: 4688
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1256 1288 3827 3859 1256
Size: 32 2539 32 2085
Fragments arranged by size:
2539 2085 32 32
PspOMI G'GGCC_C
Cuts at: 272 272
Size: 4688
PstI C_TGCA'G
Cuts at: 599 767 1402 1735 2420 599
Size: 168 635 333 685 2867
Fragments arranged by size:
2867 685 635 333 168
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4688
PvuII CAG'CTG
Cuts at: 762 2113 2473 762
Size: 1351 360 2977
Fragments arranged by size:
2977 1351 360
RsrII CG'GwC_CG
Cuts at: 2883 2883
Size: 4688
SacI G_AGCT'C
Cuts at: 1088 1702 1088
Size: 614 4074
SalI G'TCGA_C
Cuts at: 1725 1725
Size: 4688
SapI GCTCTTCn'nnn_
Cuts at: 215 845 2711 2921 215
Size: 630 1866 210 1982
Fragments arranged by size:
1982 1866 630 210
SbfI CC_TGCA'GG
Cuts at: 1735 1735
Size: 4688
ScaI AGT'ACT
Cuts at: 4205 4205
Size: 4688
SexAI A'CCwGG_T
Cuts at: 149 149
Size: 4688
SfcI C'TryA_G
Cuts at: 33 595 763 1398 1486 1731 2416 3409 3600
Size: 562 168 635 88 245 685 993 191
Cuts at: 3600 33
Size: 1121
Fragments arranged by size:
1121 993 685 635 562 245 191 168
88
SfoI GGC'GCC
Cuts at: 2120 2368 2120
Size: 248 4440
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4688
SgrAI Cr'CCGG_yG
Cuts at: 479 479
Size: 4688
SmaI CCC'GGG
Cuts at: 1710 1710
Size: 4688
SmlI C'TyrA_G
Cuts at: 380 443 1087 1843 2758 3250 3512 380
Size: 63 644 756 915 492 262 1556
Fragments arranged by size:
1556 915 756 644 492 262 63
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4688
SphI G_CATG'C
Cuts at: 1741 2241 1741
Size: 500 4188
StyI C'CwwG_G
Cuts at: 107 888 1337 1473 1822 2176 107
Size: 781 449 136 349 354 2619
Fragments arranged by size:
2619 781 449 354 349 136
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 772 1617 2271 2404 2863 3072 772
Size: 845 654 133 459 209 2388
Fragments arranged by size:
2388 845 654 459 209 133
TatI w'GTAC_w
Cuts at: 467 1205 4203 467
Size: 738 2998 952
Fragments arranged by size:
2998 952 738
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4205 4237 4205
Size: 32 4656
Tth111I GACn'n_nGTC
Cuts at: 1246 1578 2485 1246
Size: 332 907 3449
Fragments arranged by size:
3449 907 332
XbaI T'CTAG_A
Cuts at: 1719 1719
Size: 4688
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 687 2183 687
Size: 1496 3192
XhoI C'TCGA_G
Cuts at: 380 380
Size: 4688
XmaI C'CCGG_G
Cuts at: 1708 1708
Size: 4688
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PmeI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AscI AvrII BaeI
BbsI BcgI BfrBI BmtI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PfoI PmlI PpuMI PshAI
PsiI PspXI PsrI SacII SanDI SfiI SnaBI SrfI
SspI StuI SwaI XmnI ZraI