Restriction map for
pF1KE0321
(Circular) (Six-base) MAPSORT of: pF1KE0321.seq Check: 2930 from: 1 to: 5771
pF1KE0321 5771 bp
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1767 1767
Size: 5771
AccI GT'mk_AC
Cuts at: 2322 2809 2322
Size: 487 5284
Acc65I G'GTAC_C
Cuts at: 2787 2787
Size: 5771
AclI AA'CG_TT
Cuts at: 5611 5611
Size: 5771
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 321 1075 1286 2504 3596 4028 4775 5043 321
Size: 754 211 1218 1092 432 747 268 1049
Fragments arranged by size:
1218 1092 1049 754 747 432 268 211
AfeI AGC'GCT
Cuts at: 628 628
Size: 5771
AflII C'TTAA_G
Cuts at: 846 846
Size: 5771
AflIII A'CryG_T
Cuts at: 272 1893 3123 4227 5204 272
Size: 1621 1230 1104 977 839
Fragments arranged by size:
1621 1230 1104 977 839
AgeI A'CCGG_T
Cuts at: 4159 4159
Size: 5771
AleI CACnn'nnGTG
Cuts at: 1471 1471
Size: 5771
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1430 1430
Size: 5771
AlwNI CAG_nnn'CTG
Cuts at: 596 606 1053 1169 1357 2235 2392 3076 4643
Size: 10 447 116 188 878 157 684 1567
Cuts at: 4643 596
Size: 1724
Fragments arranged by size:
1724 1567 878 684 447 188 157 116
10
ApaI G_GGCC'C
Cuts at: 939 2188 939
Size: 1249 4522
ApaLI G'TGCA_C
Cuts at: 1120 4541 1120
Size: 3421 2350
ApoI r'AATT_y
Cuts at: 304 1509 2076 2094 2775 5124 5380 304
Size: 1205 567 18 681 2349 256 695
Fragments arranged by size:
2349 1205 695 681 567 256 18
AseI AT'TA_AT
Cuts at: 20 20
Size: 5771
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5771
AvaI C'yCGr_G
Cuts at: 932 1565 2759 2791 932
Size: 633 1194 32 3912
Fragments arranged by size:
3912 1194 633 32
BamHI G'GATC_C
Cuts at: 2575 2796 2575
Size: 221 5550
BanI G'GyrC_C
Cuts at: 2787 3201 3449 3484 2787
Size: 414 248 35 5074
Fragments arranged by size:
5074 414 248 35
BanII G_rGCy'C
Cuts at: 692 773 939 2152 2188 2201 2317 2433 2488
Size: 81 166 1213 36 13 116 116 55
Cuts at: 2488 2785 3815 692
Size: 297 1030 2648
Fragments arranged by size:
2648 1213 1030 297 166 116 116 81
55 36 13
BbeI G_GCGC'C
Cuts at: 3205 3453 3205
Size: 248 5523
BbsI GAAGACnn'nnnn_
Cuts at: 882 882
Size: 5771
BciVI GTATCCnnnnn_n'
Cuts at: 660 3669 4430 5771 660
Size: 3009 761 1341 660
Fragments arranged by size:
3009 1341 761 660
BclI T'GATC_A
Cuts at: 3291 3291
Size: 5771
BglI GCCn_nnn'nGGC
Cuts at: 453 2133 2619 453
Size: 1680 486 3605
Fragments arranged by size:
3605 1680 486
BglII A'GATC_T
Cuts at: 723 3286 5323 723
Size: 2563 2037 1171
Fragments arranged by size:
2563 2037 1171
BlpI GC'TnA_GC
Cuts at: 2883 2883
Size: 5771
Bme1580I G_kGCm'C
Cuts at: 939 1124 1244 2025 2188 3396 3489 4545 939
Size: 185 120 781 163 1208 93 1056 2165
Fragments arranged by size:
2165 1208 1056 781 185 163 120 93
BmrI ACTGGGnnnn_n'
Cuts at: 1157 2983 3064 3399 5543 1157
Size: 1826 81 335 2144 1385
Fragments arranged by size:
2144 1826 1385 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4138 4138
Size: 5771
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1222 1734 2396 2447 2587 1222
Size: 512 662 51 140 4406
Fragments arranged by size:
4406 662 512 140 51
Bpu10I CC'TnA_GC
Cuts at: 1915 1915
Size: 5771
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2034 2947 3826 4318 4616 2034
Size: 913 879 492 298 3189
Fragments arranged by size:
3189 913 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 470 1562 5475 470
Size: 1092 3913 766
Fragments arranged by size:
3913 1092 766
BsaAI yAC'GTr
Cuts at: 171 1027 3124 171
Size: 856 2097 2818
Fragments arranged by size:
2818 2097 856
BsaBI GATnn'nnATC
Cuts at: 6 3285 5322 6
Size: 3279 2037 455
Fragments arranged by size:
3279 2037 455
BsaHI Gr'CG_yC
Cuts at: 223 3202 3450 4152 5648 223
Size: 2979 248 702 1496 346
Fragments arranged by size:
2979 1496 702 346 248
BsaWI w'CCGG_w
Cuts at: 633 2960 3481 4159 4433 4580 4991 633
Size: 2327 521 678 274 147 411 1413
Fragments arranged by size:
2327 1413 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 568 598 2739 2769 568
Size: 30 2141 30 3570
Fragments arranged by size:
3570 2141 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2289 2289
Size: 5771
BseYI C'CCAG_C
Cuts at: 461 2101 2181 2271 2292 2310 2571 3196 3954
Size: 1640 80 90 21 18 261 625 758
Cuts at: 3954 4531 5027 461
Size: 577 496 1205
Fragments arranged by size:
1640 1205 758 625 577 496 261 90
80 21 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1057 1057
Size: 5771
BsiEI CG_ry'CG
Cuts at: 104 1605 3313 3359 4567 104
Size: 1501 1708 46 1208 1308
Fragments arranged by size:
1708 1501 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 692 1124 1157 1468 1856 2433 2785 3563 3753
Size: 432 33 311 388 577 352 778 190
Cuts at: 3753 4545 692
Size: 792 1918
Fragments arranged by size:
1918 792 778 577 432 388 352 311
190 33
BsmI GAATG_Cn'
Cuts at: 359 470 359
Size: 111 5660
BsmBI CGTCTCn'nnnn_
Cuts at: 496 2211 496
Size: 1715 4056
Bsp1286I G_dGCh'C
Cuts at: 692 773 939 1124 1157 1244 1468 1856 2025
Size: 81 166 185 33 87 224 388 169
Cuts at: 2025 2152 2188 2201 2317 2433 2488 2785 3396
Size: 127 36 13 116 116 55 297 611
Cuts at: 3396 3489 3563 3753 3815 4545 692
Size: 93 74 190 62 730 1918
Fragments arranged by size:
1918 730 611 388 297 224 190 185
169 166 127 116 116 93 87 81
74 62 55 36 33 13
BspEI T'CCGG_A
Cuts at: 633 4991 633
Size: 4358 1413
BspHI T'CATG_A
Cuts at: 4947 4947
Size: 5771
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1412 1767 2821 3035 3337 3718 1412
Size: 355 1054 214 302 381 3465
Fragments arranged by size:
3465 1054 381 355 302 214
BsrBI CCG'CTC
Cuts at: 50 2627 4063 4117 5601 5766 50
Size: 2577 1436 54 1484 165 55
Fragments arranged by size:
2577 1484 1436 165 55 54
BsrDI GCAATG_nn'
Cuts at: 242 1796 3683 242
Size: 1554 1887 2330
Fragments arranged by size:
2330 1887 1554
BsrFI r'CCGG_y
Cuts at: 479 1737 2038 2050 3769 3950 4159 479
Size: 1258 301 12 1719 181 209 2091
Fragments arranged by size:
2091 1719 1258 301 209 181 12
BssSI C'ACGA_G
Cuts at: 4042 4400 4042
Size: 358 5413
BstAPI GCAn_nnn'nTGC
Cuts at: 3148 3148
Size: 5771
BstBI TT'CG_AA
Cuts at: 1959 4132 1959
Size: 2173 3598
BstEII G'GTnAC_C
Cuts at: 2166 2166
Size: 5771
BstXI CCAn_nnnn'nTGG
Cuts at: 1711 1711
Size: 5771
BstYI r'GATC_y
Cuts at: 1 723 2575 2796 3263 3286 3621 3867 4879
Size: 722 1852 221 467 23 335 246 1012
Cuts at: 4879 4965 4977 5323 1
Size: 86 12 346 449
Fragments arranged by size:
1852 1012 722 467 449 346 335 246
221 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1626 2603 2714 1626
Size: 977 111 4683
Fragments arranged by size:
4683 977 111
BtgI C'CryG_G
Cuts at: 107 546 747 1828 2617 107
Size: 439 201 1081 789 3261
Fragments arranged by size:
3261 1081 789 439 201
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3721 3902 4065 3721
Size: 181 163 5427
Fragments arranged by size:
5427 181 163
BtsI GCAGTG_nn'
Cuts at: 442 2565 3014 442
Size: 2123 449 3199
Fragments arranged by size:
3199 2123 449
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1362 1397 1362
Size: 35 5736
DraI TTT'AAA
Cuts at: 2770 2770
Size: 5771
DraIII CAC_nnn'GTG
Cuts at: 1119 2178 1119
Size: 1059 4712
DrdI GACnn_nn'nnGTC
Cuts at: 3477 4335 3477
Size: 858 4913
EaeI y'GGCC_r
Cuts at: 454 1079 1581 1602 1793 3356 3530 3921 3948
Size: 625 502 21 191 1563 174 391 27
Cuts at: 3948 5293 454
Size: 1345 932
Fragments arranged by size:
1563 1345 932 625 502 391 191 174
27 21
EagI C'GGCC_G
Cuts at: 1602 3356 1602
Size: 1754 4017
EarI CTCTTCn'nnn_
Cuts at: 255 961 1340 1942 2523 3794 4004 255
Size: 706 379 602 581 1271 210 2022
Fragments arranged by size:
2022 1271 706 602 581 379 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1831 3979 4289 4435 5581 1831
Size: 2148 310 146 1146 2021
Fragments arranged by size:
2148 2021 1146 310 146
EcoICRI GAG'CTC
Cuts at: 690 2431 2783 690
Size: 1741 352 3678
Fragments arranged by size:
3678 1741 352
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 321 1075 1222 1286 1734 2396 2447 2504 2587
Size: 754 147 64 448 662 51 57 83
Cuts at: 2587 3596 4028 4775 5043 321
Size: 1009 432 747 268 1049
Fragments arranged by size:
1049 1009 754 747 662 448 432 268
147 83 64 57 51
EcoO109I rG'GnC_Cy
Cuts at: 1953 2185 2524 2612 2698 2910 1953
Size: 232 339 88 86 212 4814
Fragments arranged by size:
4814 339 232 212 88 86
EcoRI G'AATT_C
Cuts at: 1509 2094 2775 1509
Size: 585 681 4505
Fragments arranged by size:
4505 681 585
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1120 3790 1120
Size: 2670 3101
FspI TGC'GCA
Cuts at: 439 3552 439
Size: 3113 2658
FspAI rTGC'GCAy
Cuts at: 439 439
Size: 5771
HaeII r_GCGC'y
Cuts at: 630 3205 3453 4475 5454 630
Size: 2575 248 1022 979 947
Fragments arranged by size:
2575 1022 979 947 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 250 282 862 894 1570 1602 2415 2447 2560
Size: 32 580 32 676 32 813 32 113
Cuts at: 2560 2592 2659 2691 2739 2771 3613 3643 3645
Size: 32 67 32 48 32 842 30 2
Cuts at: 3645 3675 250
Size: 30 2346
Fragments arranged by size:
2346 842 813 676 580 113 67 48
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1941 2810 1941
Size: 869 4902
KasI G'GCGC_C
Cuts at: 3201 3449 3201
Size: 248 5523
KpnI G_GTAC'C
Cuts at: 2791 2791
Size: 5771
MluI A'CGCG_T
Cuts at: 5204 5204
Size: 5771
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 293 942 4442 4626 293
Size: 649 3500 184 1438
Fragments arranged by size:
3500 1438 649 184
MscI TGG'CCA
Cuts at: 1583 1795 3532 1583
Size: 212 1737 3822
Fragments arranged by size:
3822 1737 212
MslI CAynn'nnrTG
Cuts at: 1412 1471 1653 1709 3760 3887 1412
Size: 59 182 56 2051 127 3296
Fragments arranged by size:
3296 2051 182 127 59 56
MspA1I CmG'CkG
Cuts at: 1249 2882 3196 3556 4569 4814 1249
Size: 1633 314 360 1013 245 2206
Fragments arranged by size:
2206 1633 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1739 3952 1739
Size: 2213 3558
NarI GG'CG_CC
Cuts at: 3202 3450 3202
Size: 248 5523
NcoI C'CATG_G
Cuts at: 107 747 107
Size: 640 5131
NgoMIV G'CCGG_C
Cuts at: 1737 3950 1737
Size: 2213 3558
NspI r_CATG'y
Cuts at: 1417 1485 1897 2824 3324 4231 1417
Size: 68 412 927 500 907 2957
Fragments arranged by size:
2957 927 907 500 412 68
PasI CC'CwG_GG
Cuts at: 417 865 417
Size: 448 5323
PciI A'CATG_T
Cuts at: 1893 4227 1893
Size: 2334 3437
PflMI CCAn_nnn'nTGG
Cuts at: 275 423 275
Size: 148 5623
PfoI T'CCnGG_A
Cuts at: 2677 2677
Size: 5771
PmeI GTTT'AAAC
Cuts at: 2770 2770
Size: 5771
PmlI CAC'GTG
Cuts at: 171 1027 171
Size: 856 4915
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1286 1318 4910 4942 1286
Size: 32 3592 32 2115
Fragments arranged by size:
3592 2115 32 32
PpuMI rG'GwC_Cy
Cuts at: 2698 2698
Size: 5771
PspOMI G'GGCC_C
Cuts at: 935 2184 935
Size: 1249 4522
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1033 1065 1033
Size: 32 5739
PstI C_TGCA'G
Cuts at: 2133 2253 2818 3503 2133
Size: 120 565 685 4401
Fragments arranged by size:
4401 685 565 120
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5771
PvuII CAG'CTG
Cuts at: 1249 3196 3556 1249
Size: 1947 360 3464
Fragments arranged by size:
3464 1947 360
RsrII CG'GwC_CG
Cuts at: 3966 3966
Size: 5771
SacI G_AGCT'C
Cuts at: 692 2433 2785 692
Size: 1741 352 3678
Fragments arranged by size:
3678 1741 352
SalI G'TCGA_C
Cuts at: 2808 2808
Size: 5771
SanDI GG'GwC_CC
Cuts at: 2698 2698
Size: 5771
SapI GCTCTTCn'nnn_
Cuts at: 2523 3794 4004 2523
Size: 1271 210 4290
Fragments arranged by size:
4290 1271 210
SbfI CC_TGCA'GG
Cuts at: 2133 2253 2818 2133
Size: 120 565 5086
Fragments arranged by size:
5086 565 120
ScaI AGT'ACT
Cuts at: 5288 5288
Size: 5771
SfcI C'TryA_G
Cuts at: 33 588 2129 2249 2655 2814 3499 4492 4683
Size: 555 1541 120 406 159 685 993 191
Cuts at: 4683 33
Size: 1121
Fragments arranged by size:
1541 1121 993 685 555 406 191 159
120
SfiI GGCCn_nnn'nGGCC
Cuts at: 2619 2619
Size: 5771
SfoI GGC'GCC
Cuts at: 3203 3451 3203
Size: 248 5523
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5771
SmaI CCC'GGG
Cuts at: 934 2761 2793 934
Size: 1827 32 3912
Fragments arranged by size:
3912 1827 32
SmlI C'TyrA_G
Cuts at: 846 2013 2926 3841 4333 4595 846
Size: 1167 913 915 492 262 2022
Fragments arranged by size:
2022 1167 915 913 492 262
SpeI A'CTAG_T
Cuts at: 67 2281 67
Size: 2214 3557
SphI G_CATG'C
Cuts at: 2824 3324 2824
Size: 500 5271
StuI AGG'CCT
Cuts at: 1913 2144 1913
Size: 231 5540
StyI C'CwwG_G
Cuts at: 107 747 2702 2905 3259 107
Size: 640 1955 203 354 2619
Fragments arranged by size:
2619 1955 640 354 203
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 878 1483 1924 1983 2185 3354 3487 3946 4155
Size: 605 441 59 202 1169 133 459 209
Cuts at: 4155 878
Size: 2494
Fragments arranged by size:
2494 1169 605 459 441 209 202 133
59
TatI w'GTAC_w
Cuts at: 858 1842 5286 858
Size: 984 3444 1343
Fragments arranged by size:
3444 1343 984
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5288 5320 5288
Size: 32 5739
Tth111I GACn'n_nGTC
Cuts at: 3568 3568
Size: 5771
XbaI T'CTAG_A
Cuts at: 777 2802 777
Size: 2025 3746
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1758 1803 3266 1758
Size: 45 1463 4263
Fragments arranged by size:
4263 1463 45
XmaI C'CCGG_G
Cuts at: 932 2759 2791 932
Size: 1827 32 3912
Fragments arranged by size:
3912 1827 32
XmnI GAAnn'nnTTC
Cuts at: 470 470
Size: 5771
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI PsrI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AhdI AloI AscI AvrII BaeI BbvCI BcgI
BfrBI BmgBI BmtI BsiWI BsrGI BssHII BstZ17I ClaI
EcoNI EcoRV FseI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PshAI PsiI PspXI SacII
SexAI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI