Restriction map for
pF1KE0324
(Circular) (Six-base) MAPSORT of: pF1KE0324.seq Check: 5885 from: 1 to: 4967
pF1KE0324 4967 bp
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AccI GT'mk_AC
Cuts at: 520 2005 520
Size: 1485 3482
Acc65I G'GTAC_C
Cuts at: 1983 1983
Size: 4967
AclI AA'CG_TT
Cuts at: 4807 4807
Size: 4967
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 583 1280 1396 1577 2792 3224 3971 4239 583
Size: 697 116 181 1215 432 747 268 1311
Fragments arranged by size:
1311 1215 747 697 432 268 181 116
AflIII A'CryG_T
Cuts at: 2319 3423 4400 2319
Size: 1104 977 2886
Fragments arranged by size:
2886 1104 977
AgeI A'CCGG_T
Cuts at: 3355 3355
Size: 4967
AlwNI CAG_nnn'CTG
Cuts at: 2272 3839 2272
Size: 1567 3400
ApaI G_GGCC'C
Cuts at: 623 623
Size: 4967
ApaLI G'TGCA_C
Cuts at: 3737 3737
Size: 4967
ApoI r'AATT_y
Cuts at: 190 1451 1546 1971 4320 4576 190
Size: 1261 95 425 2349 256 581
Fragments arranged by size:
2349 1261 581 425 256 95
AseI AT'TA_AT
Cuts at: 20 20
Size: 4967
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4967
AvaI C'yCGr_G
Cuts at: 212 1987 212
Size: 1775 3192
BamHI G'GATC_C
Cuts at: 125 1992 125
Size: 1867 3100
BanI G'GyrC_C
Cuts at: 1235 1983 2397 2645 2680 1235
Size: 748 414 248 35 3522
Fragments arranged by size:
3522 748 414 248 35
BanII G_rGCy'C
Cuts at: 581 623 1876 1981 3011 581
Size: 42 1253 105 1030 2537
Fragments arranged by size:
2537 1253 1030 105 42
BbeI G_GCGC'C
Cuts at: 2401 2649 2401
Size: 248 4719
BbsI GAAGACnn'nnnn_
Cuts at: 163 163
Size: 4967
BciVI GTATCCnnnnn_n'
Cuts at: 1853 2865 3626 4967 1853
Size: 1012 761 1341 1853
Fragments arranged by size:
1853 1341 1012 761
BclI T'GATC_A
Cuts at: 696 2487 696
Size: 1791 3176
BfrBI ATG'CAT
Cuts at: 1021 1021
Size: 4967
BglII A'GATC_T
Cuts at: 2482 4519 2482
Size: 2037 2930
BlpI GC'TnA_GC
Cuts at: 2079 2079
Size: 4967
Bme1580I G_kGCm'C
Cuts at: 623 2592 2685 3741 623
Size: 1969 93 1056 1849
Fragments arranged by size:
1969 1849 1056 93
BmgBI CAC'GTC
Cuts at: 120 120
Size: 4967
BmrI ACTGGGnnnn_n'
Cuts at: 1194 1796 2179 2260 2595 4739 1194
Size: 602 383 81 335 2144 1422
Fragments arranged by size:
2144 1422 602 383 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3334 3334
Size: 4967
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 805 805
Size: 4967
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 761 1274 1852 2143 3022 3514 3812 761
Size: 513 578 291 879 492 298 1916
Fragments arranged by size:
1916 879 578 513 492 298 291
BsaI GGTCTCn'nnnn_
Cuts at: 487 4671 487
Size: 4184 783
BsaAI yAC'GTr
Cuts at: 1851 2320 1851
Size: 469 4498
BsaBI GATnn'nnATC
Cuts at: 6 2481 4518 6
Size: 2475 2037 455
Fragments arranged by size:
2475 2037 455
BsaHI Gr'CG_yC
Cuts at: 2398 2646 3348 4844 2398
Size: 248 702 1496 2521
Fragments arranged by size:
2521 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2156 2677 3355 3629 3776 4187 2156
Size: 521 678 274 147 411 2936
Fragments arranged by size:
2936 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 652 764 652
Size: 112 4855
BseYI C'CCAG_C
Cuts at: 2392 3150 3727 4223 2392
Size: 758 577 496 3136
Fragments arranged by size:
3136 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2509 2555 3763 104
Size: 2405 46 1208 1308
Fragments arranged by size:
2405 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 581 1876 1981 2759 2949 3741 581
Size: 1295 105 778 190 792 1807
Fragments arranged by size:
1807 1295 792 778 190 105
BsmI GAATG_Cn'
Cuts at: 369 369
Size: 4967
Bsp1286I G_dGCh'C
Cuts at: 581 623 1876 1981 2592 2685 2759 2949 3011
Size: 42 1253 105 611 93 74 190 62
Cuts at: 3011 3741 581
Size: 730 1807
Fragments arranged by size:
1807 1253 730 611 190 105 93 74
62 42
BspEI T'CCGG_A
Cuts at: 4187 4187
Size: 4967
BspHI T'CATG_A
Cuts at: 4143 4143
Size: 4967
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1340 1706 2017 2231 2533 2914 1340
Size: 366 311 214 302 381 3393
Fragments arranged by size:
3393 381 366 311 302 214
BsrBI CCG'CTC
Cuts at: 50 3259 3313 4797 4962 50
Size: 3209 54 1484 165 55
Fragments arranged by size:
3209 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2879 2879
Size: 4967
BsrFI r'CCGG_y
Cuts at: 2965 3146 3355 2965
Size: 181 209 4577
Fragments arranged by size:
4577 209 181
BsrGI T'GTAC_A
Cuts at: 1227 1937 1227
Size: 710 4257
BssSI C'ACGA_G
Cuts at: 3238 3596 3238
Size: 358 4609
BstAPI GCAn_nnn'nTGC
Cuts at: 2344 2344
Size: 4967
BstBI TT'CG_AA
Cuts at: 3328 3328
Size: 4967
BstYI r'GATC_y
Cuts at: 1 125 1992 2459 2482 2817 3063 4075 4161
Size: 124 1867 467 23 335 246 1012 86
Cuts at: 4161 4173 4519 1
Size: 12 346 449
Fragments arranged by size:
1867 1012 467 449 346 335 246 124
86 23 12
BstZ17I GTA'TAC
Cuts at: 521 521
Size: 4967
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2917 3098 3261 2917
Size: 181 163 4623
Fragments arranged by size:
4623 181 163
BtsI GCAGTG_nn'
Cuts at: 2210 2210
Size: 4967
DraI TTT'AAA
Cuts at: 687 1107 1966 687
Size: 420 859 3688
Fragments arranged by size:
3688 859 420
DrdI GACnn_nn'nnGTC
Cuts at: 2673 3531 2673
Size: 858 4109
EaeI y'GGCC_r
Cuts at: 1835 2552 2726 3117 3144 4489 1835
Size: 717 174 391 27 1345 2313
Fragments arranged by size:
2313 1345 717 391 174 27
EagI C'GGCC_G
Cuts at: 2552 2552
Size: 4967
EarI CTCTTCn'nnn_
Cuts at: 194 1424 2990 3200 194
Size: 1230 1566 210 1961
Fragments arranged by size:
1961 1566 1230 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3175 3485 3631 4777 3175
Size: 310 146 1146 3365
Fragments arranged by size:
3365 1146 310 146
EcoICRI GAG'CTC
Cuts at: 579 1874 1979 579
Size: 1295 105 3567
Fragments arranged by size:
3567 1295 105
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 583 805 1280 1396 1577 2792 3224 3971 4239
Size: 222 475 116 181 1215 432 747 268
Cuts at: 4239 583
Size: 1311
Fragments arranged by size:
1311 1215 747 475 432 268 222 181
116
EcoO109I rG'GnC_Cy
Cuts at: 619 620 2106 619
Size: 1 1486 3480
Fragments arranged by size:
3480 1486 1
EcoRI G'AATT_C
Cuts at: 1971 1971
Size: 4967
EcoRV GAT'ATC
Cuts at: 1533 1812 1533
Size: 279 4688
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 622 1665 1830 2986 622
Size: 1043 165 1156 2603
Fragments arranged by size:
2603 1156 1043 165
FspI TGC'GCA
Cuts at: 2748 2748
Size: 4967
HaeII r_GCGC'y
Cuts at: 2401 2649 3671 4650 2401
Size: 248 1022 979 2718
Fragments arranged by size:
2718 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 159 191 1270 1302 1453 1485 2809 2839 2841
Size: 32 1079 32 151 32 1324 30 2
Cuts at: 2841 2871 159
Size: 30 2255
Fragments arranged by size:
2255 1324 1079 151 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 360 845 2006 360
Size: 485 1161 3321
Fragments arranged by size:
3321 1161 485
HindIII A'AGCT_T
Cuts at: 1292 1292
Size: 4967
HpaI GTT'AAC
Cuts at: 360 360
Size: 4967
KasI G'GCGC_C
Cuts at: 2397 2645 2397
Size: 248 4719
KpnI G_GTAC'C
Cuts at: 1987 1987
Size: 4967
MluI A'CGCG_T
Cuts at: 4400 4400
Size: 4967
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 286 3638 3822 286
Size: 3352 184 1431
Fragments arranged by size:
3352 1431 184
MscI TGG'CCA
Cuts at: 1837 2728 1837
Size: 891 4076
MslI CAynn'nnrTG
Cuts at: 818 2956 3083 818
Size: 2138 127 2702
Fragments arranged by size:
2702 2138 127
MspA1I CmG'CkG
Cuts at: 238 1233 1300 2078 2392 2752 3765 4010 238
Size: 995 67 778 314 360 1013 245 1195
Fragments arranged by size:
1195 1013 995 778 360 314 245 67
NaeI GCC'GGC
Cuts at: 3148 3148
Size: 4967
NarI GG'CG_CC
Cuts at: 2398 2646 2398
Size: 248 4719
NgoMIV G'CCGG_C
Cuts at: 3146 3146
Size: 4967
NsiI A_TGCA'T
Cuts at: 1023 1023
Size: 4967
NspI r_CATG'y
Cuts at: 2020 2520 3427 2020
Size: 500 907 3560
Fragments arranged by size:
3560 907 500
PciI A'CATG_T
Cuts at: 3423 3423
Size: 4967
PflMI CCAn_nnn'nTGG
Cuts at: 1883 1883
Size: 4967
PmeI GTTT'AAAC
Cuts at: 1966 1966
Size: 4967
PmlI CAC'GTG
Cuts at: 1851 1851
Size: 4967
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4106 4138 4106
Size: 32 4935
PspOMI G'GGCC_C
Cuts at: 619 619
Size: 4967
PstI C_TGCA'G
Cuts at: 1337 1747 2014 2699 1337
Size: 410 267 685 3605
Fragments arranged by size:
3605 685 410 267
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4967
PvuII CAG'CTG
Cuts at: 238 1300 2392 2752 238
Size: 1062 1092 360 2453
Fragments arranged by size:
2453 1092 1062 360
RsrII CG'GwC_CG
Cuts at: 3162 3162
Size: 4967
SacI G_AGCT'C
Cuts at: 581 1876 1981 581
Size: 1295 105 3567
Fragments arranged by size:
3567 1295 105
SalI G'TCGA_C
Cuts at: 2004 2004
Size: 4967
SapI GCTCTTCn'nnn_
Cuts at: 1424 2990 3200 1424
Size: 1566 210 3191
Fragments arranged by size:
3191 1566 210
SbfI CC_TGCA'GG
Cuts at: 2014 2014
Size: 4967
ScaI AGT'ACT
Cuts at: 4484 4484
Size: 4967
SfcI C'TryA_G
Cuts at: 33 614 1333 1743 2010 2695 3688 3879 33
Size: 581 719 410 267 685 993 191 1121
Fragments arranged by size:
1121 993 719 685 581 410 267 191
SfoI GGC'GCC
Cuts at: 2399 2647 2399
Size: 248 4719
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4967
SmaI CCC'GGG
Cuts at: 214 1989 214
Size: 1775 3192
SmlI C'TyrA_G
Cuts at: 776 1289 1867 2122 3037 3529 3791 776
Size: 513 578 255 915 492 262 1952
Fragments arranged by size:
1952 915 578 513 492 262 255
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4967
SphI G_CATG'C
Cuts at: 2020 2520 2020
Size: 500 4467
SspI AAT'ATT
Cuts at: 449 964 1246 1271 449
Size: 515 282 25 4145
Fragments arranged by size:
4145 515 282 25
StyI C'CwwG_G
Cuts at: 1126 2101 2455 1126
Size: 975 354 3638
Fragments arranged by size:
3638 975 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2550 2683 3142 3351 2550
Size: 133 459 209 4166
Fragments arranged by size:
4166 459 209 133
TatI w'GTAC_w
Cuts at: 626 1227 1937 4482 626
Size: 601 710 2545 1111
Fragments arranged by size:
2545 1111 710 601
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 866 898 4484 4516 866
Size: 32 3586 32 1317
Fragments arranged by size:
3586 1317 32 32
Tth111I GACn'n_nGTC
Cuts at: 2764 2764
Size: 4967
XbaI T'CTAG_A
Cuts at: 1998 1998
Size: 4967
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2462 2462
Size: 4967
XmaI C'CCGG_G
Cuts at: 212 1987 212
Size: 1775 3192
XmnI GAAnn'nnTTC
Cuts at: 191 203 308 191
Size: 12 105 4850
Fragments arranged by size:
4850 105 12
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstYI BstZ17I BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NsiI NspI PciI PflMI PmeI PmlI
PpiI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbvCI BcgI BglI BmtI Bpu10I
BsaXI BsgI BsiWI BsmBI BssHII BstEII BstXI Bsu36I
BtgI ClaI CspCI DraIII EcoNI FseI FspAI MfeI
NcoI NdeI NheI NotI NruI PacI PasI PfoI
PpuMI PshAI PsiI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI StuI SwaI XhoI ZraI