Restriction map for
pF1KE0325
(Circular) (Six-base) MAPSORT of: pF1KE0325.seq Check: 6608 from: 1 to: 4625
pF1KE0325 4625 bp
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 461 461
Size: 4625
AatII G_ACGT'C
Cuts at: 1199 1199
Size: 4625
AccI GT'mk_AC
Cuts at: 1663 1663
Size: 4625
Acc65I G'GTAC_C
Cuts at: 1641 1641
Size: 4625
AclI AA'CG_TT
Cuts at: 4465 4465
Size: 4625
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 849 905 2450 2882 3629 3897 849
Size: 56 1545 432 747 268 1577
Fragments arranged by size:
1577 1545 747 432 268 56
AflIII A'CryG_T
Cuts at: 1977 3081 4058 1977
Size: 1104 977 2544
Fragments arranged by size:
2544 1104 977
AgeI A'CCGG_T
Cuts at: 3013 3013
Size: 4625
AlwNI CAG_nnn'CTG
Cuts at: 1293 1930 3497 1293
Size: 637 1567 2421
Fragments arranged by size:
2421 1567 637
ApaI G_GGCC'C
Cuts at: 220 220
Size: 4625
ApaLI G'TGCA_C
Cuts at: 3395 3395
Size: 4625
ApoI r'AATT_y
Cuts at: 1629 3978 4234 1629
Size: 2349 256 2020
Fragments arranged by size:
2349 2020 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4625
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4625
AvaI C'yCGr_G
Cuts at: 1009 1645 1009
Size: 636 3989
BamHI G'GATC_C
Cuts at: 1650 1650
Size: 4625
BanI G'GyrC_C
Cuts at: 114 144 1414 1641 2055 2303 2338 114
Size: 30 1270 227 414 248 35 2401
Fragments arranged by size:
2401 1270 414 248 227 35 30
BanII G_rGCy'C
Cuts at: 220 1037 1639 2669 220
Size: 817 602 1030 2176
Fragments arranged by size:
2176 1030 817 602
BbeI G_GCGC'C
Cuts at: 118 2059 2307 118
Size: 1941 248 2436
Fragments arranged by size:
2436 1941 248
BbsI GAAGACnn'nnnn_
Cuts at: 307 307
Size: 4625
BbvCI CC'TCA_GC
Cuts at: 331 331
Size: 4625
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 202 236 202
Size: 34 4591
BciVI GTATCCnnnnn_n'
Cuts at: 2523 3284 4625 2523
Size: 761 1341 2523
Fragments arranged by size:
2523 1341 761
BclI T'GATC_A
Cuts at: 2145 2145
Size: 4625
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 4625
BglII A'GATC_T
Cuts at: 2140 4177 2140
Size: 2037 2588
BlpI GC'TnA_GC
Cuts at: 1737 1737
Size: 4625
Bme1580I G_kGCm'C
Cuts at: 149 220 824 1253 2250 2343 3399 149
Size: 71 604 429 997 93 1056 1375
Fragments arranged by size:
1375 1056 997 604 429 93 71
BmrI ACTGGGnnnn_n'
Cuts at: 1593 1837 1918 2253 4397 1593
Size: 244 81 335 2144 1821
Fragments arranged by size:
2144 1821 335 244 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 669 2992 669
Size: 2323 2302
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 420 544 661 738 420
Size: 124 117 77 4307
Fragments arranged by size:
4307 124 117 77
Bpu10I CC'TnA_GC
Cuts at: 331 331
Size: 4625
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 445 1021 1584 1801 2680 3172 3470 445
Size: 576 563 217 879 492 298 1600
Fragments arranged by size:
1600 879 576 563 492 298 217
BsaI GGTCTCn'nnnn_
Cuts at: 4329 4329
Size: 4625
BsaAI yAC'GTr
Cuts at: 819 873 1978 819
Size: 54 1105 3466
Fragments arranged by size:
3466 1105 54
BsaBI GATnn'nnATC
Cuts at: 6 2139 4176 6
Size: 2133 2037 455
Fragments arranged by size:
2133 2037 455
BsaHI Gr'CG_yC
Cuts at: 115 1196 2056 2304 3006 4502 115
Size: 1081 860 248 702 1496 238
Fragments arranged by size:
1496 1081 860 702 248 238
BsaWI w'CCGG_w
Cuts at: 1814 2335 3013 3287 3434 3845 1814
Size: 521 678 274 147 411 2594
Fragments arranged by size:
2594 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 658 658
Size: 4625
BseYI C'CCAG_C
Cuts at: 265 501 937 2050 2808 3385 3881 265
Size: 236 436 1113 758 577 496 1009
Fragments arranged by size:
1113 1009 758 577 496 436 236
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 206 915 916 1150 206
Size: 709 1 234 3681
Fragments arranged by size:
3681 709 234 1
BsiEI CG_ry'CG
Cuts at: 104 2167 2213 3421 104
Size: 2063 46 1208 1308
Fragments arranged by size:
2063 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1037 1639 2417 2607 3399 1037
Size: 602 778 190 792 2263
Fragments arranged by size:
2263 792 778 602 190
Bsp1286I G_dGCh'C
Cuts at: 149 220 824 1037 1253 1639 2250 2343 2417
Size: 71 604 213 216 386 611 93 74
Cuts at: 2417 2607 2669 3399 149
Size: 190 62 730 1375
Fragments arranged by size:
1375 730 611 604 386 216 213 190
93 74 71 62
BspEI T'CCGG_A
Cuts at: 3845 3845
Size: 4625
BspHI T'CATG_A
Cuts at: 3801 3801
Size: 4625
BspMI ACCTGCnnnn'nnnn_
Cuts at: 461 894 1373 1675 1889 2191 2572 461
Size: 433 479 302 214 302 381 2514
Fragments arranged by size:
2514 479 433 381 302 302 214
BsrBI CCG'CTC
Cuts at: 50 2917 2971 4455 4620 50
Size: 2867 54 1484 165 55
Fragments arranged by size:
2867 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 342 2537 342
Size: 2195 2430
BsrFI r'CCGG_y
Cuts at: 1601 2623 2804 3013 1601
Size: 1022 181 209 3213
Fragments arranged by size:
3213 1022 209 181
BsrGI T'GTAC_A
Cuts at: 590 1094 590
Size: 504 4121
BssSI C'ACGA_G
Cuts at: 2896 3254 2896
Size: 358 4267
BstAPI GCAn_nnn'nTGC
Cuts at: 2002 2002
Size: 4625
BstBI TT'CG_AA
Cuts at: 2986 2986
Size: 4625
BstXI CCAn_nnnn'nTGG
Cuts at: 1392 1515 1392
Size: 123 4502
BstYI r'GATC_y
Cuts at: 1 1498 1650 2117 2140 2475 2721 3733 3819
Size: 1497 152 467 23 335 246 1012 86
Cuts at: 3819 3831 4177 1
Size: 12 346 449
Fragments arranged by size:
1497 1012 467 449 346 335 246 152
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 468 914 1590 468
Size: 446 676 3503
Fragments arranged by size:
3503 676 446
BtgI C'CryG_G
Cuts at: 107 191 377 1478 107
Size: 84 186 1101 3254
Fragments arranged by size:
3254 1101 186 84
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1068 2575 2756 2919 1068
Size: 1507 181 163 2774
Fragments arranged by size:
2774 1507 181 163
BtsI GCAGTG_nn'
Cuts at: 829 1868 829
Size: 1039 3586
DraI TTT'AAA
Cuts at: 1624 1624
Size: 4625
DrdI GACnn_nn'nnGTC
Cuts at: 2331 3189 2331
Size: 858 3767
EaeI y'GGCC_r
Cuts at: 380 548 1220 2210 2384 2775 2802 4147 380
Size: 168 672 990 174 391 27 1345 858
Fragments arranged by size:
1345 990 858 672 391 174 168 27
EagI C'GGCC_G
Cuts at: 2210 2210
Size: 4625
EarI CTCTTCn'nnn_
Cuts at: 1126 2648 2858 1126
Size: 1522 210 2893
Fragments arranged by size:
2893 1522 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2833 3143 3289 4435 2833
Size: 310 146 1146 3023
Fragments arranged by size:
3023 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1035 1637 1035
Size: 602 4023
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 420 544 661 738 849 905 2450 2882 3629
Size: 124 117 77 111 56 1545 432 747
Cuts at: 3629 3897 420
Size: 268 1148
Fragments arranged by size:
1545 1148 747 432 268 124 117 111
77 56
EcoO109I rG'GnC_Cy
Cuts at: 216 217 521 1041 1764 216
Size: 1 304 520 723 3077
Fragments arranged by size:
3077 723 520 304 1
EcoRI G'AATT_C
Cuts at: 1629 1629
Size: 4625
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2644 2644
Size: 4625
FspI TGC'GCA
Cuts at: 2406 2406
Size: 4625
HaeII r_GCGC'y
Cuts at: 118 2059 2307 3329 4308 118
Size: 1941 248 1022 979 435
Fragments arranged by size:
1941 1022 979 435 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1573 1605 2467 2497 2499 2529 1573
Size: 32 862 30 2 30 3669
Fragments arranged by size:
3669 862 32 30 30 2
HincII GTy'rAC
Cuts at: 416 1664 416
Size: 1248 3377
KasI G'GCGC_C
Cuts at: 114 2055 2303 114
Size: 1941 248 2436
Fragments arranged by size:
2436 1941 248
KpnI G_GTAC'C
Cuts at: 1645 1645
Size: 4625
MluI A'CGCG_T
Cuts at: 4058 4058
Size: 4625
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1097 1136 3296 3480 1097
Size: 39 2160 184 2242
Fragments arranged by size:
2242 2160 184 39
MscI TGG'CCA
Cuts at: 382 550 2386 382
Size: 168 1836 2621
Fragments arranged by size:
2621 1836 168
MslI CAynn'nnrTG
Cuts at: 190 2614 2741 190
Size: 2424 127 2074
Fragments arranged by size:
2424 2074 127
MspA1I CmG'CkG
Cuts at: 353 421 501 758 900 1316 1736 2050 2410
Size: 68 80 257 142 416 420 314 360
Cuts at: 2410 3423 3668 353
Size: 1013 245 1310
Fragments arranged by size:
1310 1013 420 416 360 314 257 245
142 80 68
NaeI GCC'GGC
Cuts at: 1603 2806 1603
Size: 1203 3422
NarI GG'CG_CC
Cuts at: 115 2056 2304 115
Size: 1941 248 2436
Fragments arranged by size:
2436 1941 248
NcoI C'CATG_G
Cuts at: 107 191 377 107
Size: 84 186 4355
Fragments arranged by size:
4355 186 84
NgoMIV G'CCGG_C
Cuts at: 1601 2804 1601
Size: 1203 3422
NspI r_CATG'y
Cuts at: 1330 1678 2178 3085 1330
Size: 348 500 907 2870
Fragments arranged by size:
2870 907 500 348
PciI A'CATG_T
Cuts at: 3081 3081
Size: 4625
PmeI GTTT'AAAC
Cuts at: 1624 1624
Size: 4625
PmlI CAC'GTG
Cuts at: 819 873 819
Size: 54 4571
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3764 3796 3764
Size: 32 4593
PpuMI rG'GwC_Cy
Cuts at: 521 1041 521
Size: 520 4105
PshAI GACnn'nnGTC
Cuts at: 421 1234 421
Size: 813 3812
PspOMI G'GGCC_C
Cuts at: 216 216
Size: 4625
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 661 693 661
Size: 32 4593
PstI C_TGCA'G
Cuts at: 125 905 1672 2357 125
Size: 780 767 685 2393
Fragments arranged by size:
2393 780 767 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4625
PvuII CAG'CTG
Cuts at: 421 501 900 1316 2050 2410 421
Size: 80 399 416 734 360 2636
Fragments arranged by size:
2636 734 416 399 360 80
RsrII CG'GwC_CG
Cuts at: 2820 2820
Size: 4625
SacI G_AGCT'C
Cuts at: 1037 1639 1037
Size: 602 4023
SalI G'TCGA_C
Cuts at: 1662 1662
Size: 4625
SanDI GG'GwC_CC
Cuts at: 521 521
Size: 4625
SapI GCTCTTCn'nnn_
Cuts at: 2648 2858 2648
Size: 210 4415
SbfI CC_TGCA'GG
Cuts at: 1672 1672
Size: 4625
ScaI AGT'ACT
Cuts at: 4142 4142
Size: 4625
SexAI A'CCwGG_T
Cuts at: 680 680
Size: 4625
SfcI C'TryA_G
Cuts at: 33 121 253 901 1173 1668 2353 3346 3537
Size: 88 132 648 272 495 685 993 191
Cuts at: 3537 33
Size: 1121
Fragments arranged by size:
1121 993 685 648 495 272 191 132
88
SfoI GGC'GCC
Cuts at: 116 2057 2305 116
Size: 1941 248 2436
Fragments arranged by size:
2436 1941 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4625
SmaI CCC'GGG
Cuts at: 1647 1647
Size: 4625
SmlI C'TyrA_G
Cuts at: 460 1036 1563 1780 2695 3187 3449 460
Size: 576 527 217 915 492 262 1636
Fragments arranged by size:
1636 915 576 527 492 262 217
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4625
SphI G_CATG'C
Cuts at: 1678 2178 1678
Size: 500 4125
StyI C'CwwG_G
Cuts at: 107 191 377 1759 2113 107
Size: 84 186 1382 354 2619
Fragments arranged by size:
2619 1382 354 186 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2208 2341 2800 3009 2208
Size: 133 459 209 3824
Fragments arranged by size:
3824 459 209 133
TatI w'GTAC_w
Cuts at: 590 1094 1103 4140 590
Size: 504 9 3037 1075
Fragments arranged by size:
3037 1075 504 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4142 4174 4142
Size: 32 4593
Tth111I GACn'n_nGTC
Cuts at: 2422 2422
Size: 4625
XbaI T'CTAG_A
Cuts at: 1656 1656
Size: 4625
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 981 2120 981
Size: 1139 3486
XmaI C'CCGG_G
Cuts at: 1645 1645
Size: 4625
ZraI GAC'GTC
Cuts at: 1197 1197
Size: 4625
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PmeI PmlI PpiI PpuMI PshAI PspOMI PsrI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AlfI AloI AscI AvrII
BaeI BfrBI BmgBI BmtI BsaXI BsiWI BsmI BsmBI
BssHII BstEII BstZ17I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PsiI PspXI
SacII SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI