Restriction map for
pF1KE0326
(Circular) (Six-base) MAPSORT of: pF1KE0326.seq Check: 3120 from: 1 to: 5030
pF1KE0326 5030 bp
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1803 1803
Size: 5030
AccI GT'mk_AC
Cuts at: 1583 2068 1583
Size: 485 4545
Acc65I G'GTAC_C
Cuts at: 275 2046 275
Size: 1771 3259
AclI AA'CG_TT
Cuts at: 4870 4870
Size: 5030
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 287 560 796 1807 2855 3287 4034 4302 287
Size: 273 236 1011 1048 432 747 268 1015
Fragments arranged by size:
1048 1015 1011 747 432 273 268 236
AflIII A'CryG_T
Cuts at: 2382 3486 4463 2382
Size: 1104 977 2949
Fragments arranged by size:
2949 1104 977
AgeI A'CCGG_T
Cuts at: 3418 3418
Size: 5030
AlwNI CAG_nnn'CTG
Cuts at: 393 1740 1788 2335 3902 393
Size: 1347 48 547 1567 1521
Fragments arranged by size:
1567 1521 1347 547 48
ApaI G_GGCC'C
Cuts at: 906 906
Size: 5030
ApaLI G'TGCA_C
Cuts at: 3800 3800
Size: 5030
ApoI r'AATT_y
Cuts at: 2034 4383 4639 2034
Size: 2349 256 2425
Fragments arranged by size:
2425 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5030
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5030
AvaI C'yCGr_G
Cuts at: 628 1346 2050 628
Size: 718 704 3608
Fragments arranged by size:
3608 718 704
BamHI G'GATC_C
Cuts at: 2055 2055
Size: 5030
BanI G'GyrC_C
Cuts at: 275 638 892 1197 2046 2460 2708 2743 275
Size: 363 254 305 849 414 248 35 2562
Fragments arranged by size:
2562 849 414 363 305 254 248 35
BanII G_rGCy'C
Cuts at: 624 906 992 1192 1655 2044 3074 624
Size: 282 86 200 463 389 1030 2580
Fragments arranged by size:
2580 1030 463 389 282 200 86
BbeI G_GCGC'C
Cuts at: 2464 2712 2464
Size: 248 4782
BbsI GAAGACnn'nnnn_
Cuts at: 939 1066 939
Size: 127 4903
BbvCI CC'TCA_GC
Cuts at: 320 802 997 320
Size: 482 195 4353
Fragments arranged by size:
4353 482 195
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4996
BciVI GTATCCnnnnn_n'
Cuts at: 2928 3689 5030 2928
Size: 761 1341 2928
Fragments arranged by size:
2928 1341 761
BclI T'GATC_A
Cuts at: 2550 2550
Size: 5030
BfrBI ATG'CAT
Cuts at: 1916 1916
Size: 5030
BglI GCCn_nnn'nGGC
Cuts at: 480 901 977 480
Size: 421 76 4533
Fragments arranged by size:
4533 421 76
BglII A'GATC_T
Cuts at: 2545 4582 2545
Size: 2037 2993
BlpI GC'TnA_GC
Cuts at: 2142 2142
Size: 5030
Bme1580I G_kGCm'C
Cuts at: 603 764 906 1202 2655 2748 3804 603
Size: 161 142 296 1453 93 1056 1829
Fragments arranged by size:
1829 1453 1056 296 161 142 93
BmrI ACTGGGnnnn_n'
Cuts at: 519 1137 1555 1610 1875 2242 2323 2658 4802
Size: 618 418 55 265 367 81 335 2144
Cuts at: 4802 519
Size: 747
Fragments arranged by size:
2144 747 618 418 367 335 265 81
55
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1503 3397 1503
Size: 1894 3136
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 133 370 568 598 874 1006 1147 1265 133
Size: 237 198 30 276 132 141 118 3898
Fragments arranged by size:
3898 276 237 198 141 132 118 30
Bpu10I CC'TnA_GC
Cuts at: 320 802 997 1589 320
Size: 482 195 592 3761
Fragments arranged by size:
3761 592 482 195
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 805 1512 2206 3085 3577 3875 805
Size: 707 694 879 492 298 1960
Fragments arranged by size:
1960 879 707 694 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1426 4734 1426
Size: 3308 1722
BsaAI yAC'GTr
Cuts at: 577 2383 577
Size: 1806 3224
BsaBI GATnn'nnATC
Cuts at: 6 2544 4581 6
Size: 2538 2037 455
Fragments arranged by size:
2538 2037 455
BsaHI Gr'CG_yC
Cuts at: 2461 2709 3411 4907 2461
Size: 248 702 1496 2584
Fragments arranged by size:
2584 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1035 2219 2740 3418 3692 3839 4250 1035
Size: 1184 521 678 274 147 411 1815
Fragments arranged by size:
1815 1184 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 566 596 1196 1226 1533 1563 566
Size: 30 600 30 307 30 4033
Fragments arranged by size:
4033 600 307 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 562 985 562
Size: 423 4607
BseYI C'CCAG_C
Cuts at: 174 888 912 1440 2455 3213 3790 4286 174
Size: 714 24 528 1015 758 577 496 918
Fragments arranged by size:
1015 918 758 714 577 528 496 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 211 1679 1815 211
Size: 1468 136 3426
Fragments arranged by size:
3426 1468 136
BsiEI CG_ry'CG
Cuts at: 104 2572 2618 3826 104
Size: 2468 46 1208 1308
Fragments arranged by size:
2468 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 944 2044 2822 3012 3804 944
Size: 1100 778 190 792 2170
Fragments arranged by size:
2170 1100 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 603 624 764 906 944 992 1192 1202 1655
Size: 21 140 142 38 48 200 10 453
Cuts at: 1655 2044 2655 2748 2822 3012 3074 3804 603
Size: 389 611 93 74 190 62 730 1829
Fragments arranged by size:
1829 730 611 453 389 200 190 142
140 93 74 62 48 38 21 10
BspEI T'CCGG_A
Cuts at: 4250 4250
Size: 5030
BspHI T'CATG_A
Cuts at: 4206 4206
Size: 5030
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1097 1748 1803 1964 2080 2294 2596 2977 1097
Size: 651 55 161 116 214 302 381 3150
Fragments arranged by size:
3150 651 381 302 214 161 116 55
BsrBI CCG'CTC
Cuts at: 50 3322 3376 4860 5025 50
Size: 3272 54 1484 165 55
Fragments arranged by size:
3272 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1424 2942 1424
Size: 1518 3512
BsrFI r'CCGG_y
Cuts at: 1724 3028 3209 3418 1724
Size: 1304 181 209 3336
Fragments arranged by size:
3336 1304 209 181
BsrGI T'GTAC_A
Cuts at: 738 738
Size: 5030
BssSI C'ACGA_G
Cuts at: 790 3301 3659 790
Size: 2511 358 2161
Fragments arranged by size:
2511 2161 358
BstAPI GCAn_nnn'nTGC
Cuts at: 598 2407 598
Size: 1809 3221
BstBI TT'CG_AA
Cuts at: 3391 3391
Size: 5030
BstXI CCAn_nnnn'nTGG
Cuts at: 1243 1642 1704 1882 1243
Size: 399 62 178 4391
Fragments arranged by size:
4391 399 178 62
BstYI r'GATC_y
Cuts at: 1 2055 2522 2545 2880 3126 4138 4224 4236
Size: 2054 467 23 335 246 1012 86 12
Cuts at: 4236 4582 1
Size: 346 449
Fragments arranged by size:
2054 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 115 665 1408 115
Size: 550 743 3737
Fragments arranged by size:
3737 743 550
BtgI C'CryG_G
Cuts at: 401 1217 1697 401
Size: 816 480 3734
Fragments arranged by size:
3734 816 480
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 214 2980 3161 3324 214
Size: 2766 181 163 1920
Fragments arranged by size:
2766 1920 181 163
BtsI GCAGTG_nn'
Cuts at: 373 960 1722 1993 2273 373
Size: 587 762 271 280 3130
Fragments arranged by size:
3130 762 587 280 271
DraI TTT'AAA
Cuts at: 2029 2029
Size: 5030
DraIII CAC_nnn'GTG
Cuts at: 598 598
Size: 5030
DrdI GACnn_nn'nnGTC
Cuts at: 1550 2736 3594 1550
Size: 1186 858 2986
Fragments arranged by size:
2986 1186 858
EaeI y'GGCC_r
Cuts at: 398 845 1214 1574 1847 2615 2789 3180 3207
Size: 447 369 360 273 768 174 391 27
Cuts at: 3207 4552 398
Size: 1345 876
Fragments arranged by size:
1345 876 768 447 391 369 360 273
174 27
EagI C'GGCC_G
Cuts at: 2615 2615
Size: 5030
EarI CTCTTCn'nnn_
Cuts at: 329 539 3053 3263 329
Size: 210 2514 210 2096
Fragments arranged by size:
2514 2096 210 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3238 3548 3694 4840 3238
Size: 310 146 1146 3428
Fragments arranged by size:
3428 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2042 2042
Size: 5030
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 133 287 370 560 568 598 796 874 1006
Size: 154 83 190 8 30 198 78 132
Cuts at: 1006 1147 1265 1807 2855 3287 4034 4302 133
Size: 141 118 542 1048 432 747 268 861
Fragments arranged by size:
1048 861 747 542 432 268 198 190
154 141 132 118 83 78 30 8
EcoNI CCTnn'n_nnAGG
Cuts at: 586 1393 586
Size: 807 4223
EcoO109I rG'GnC_Cy
Cuts at: 354 767 902 1086 1158 2169 354
Size: 413 135 184 72 1011 3215
Fragments arranged by size:
3215 1011 413 184 135 72
EcoRI G'AATT_C
Cuts at: 2034 2034
Size: 5030
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3049 3049
Size: 5030
FspI TGC'GCA
Cuts at: 2811 2811
Size: 5030
HaeII r_GCGC'y
Cuts at: 2464 2712 3734 4713 2464
Size: 248 1022 979 2781
Fragments arranged by size:
2781 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 703 735 1637 1669 2872 2902 2904 2934 703
Size: 32 902 32 1203 30 2 30 2799
Fragments arranged by size:
2799 1203 902 32 32 30 30 2
HincII GTy'rAC
Cuts at: 554 2069 554
Size: 1515 3515
KasI G'GCGC_C
Cuts at: 2460 2708 2460
Size: 248 4782
KpnI G_GTAC'C
Cuts at: 279 2050 279
Size: 1771 3259
MluI A'CGCG_T
Cuts at: 4463 4463
Size: 5030
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1085 1534 3701 3885 1085
Size: 449 2167 184 2230
Fragments arranged by size:
2230 2167 449 184
MscI TGG'CCA
Cuts at: 847 1216 1576 1849 2791 847
Size: 369 360 273 942 3086
Fragments arranged by size:
3086 942 369 360 273
MslI CAynn'nnrTG
Cuts at: 790 3019 3146 790
Size: 2229 127 2674
Fragments arranged by size:
2674 2229 127
MspA1I CmG'CkG
Cuts at: 390 1533 2141 2455 2815 3828 4073 390
Size: 1143 608 314 360 1013 245 1347
Fragments arranged by size:
1347 1143 1013 608 360 314 245
NaeI GCC'GGC
Cuts at: 1726 3211 1726
Size: 1485 3545
NarI GG'CG_CC
Cuts at: 2461 2709 2461
Size: 248 4782
NcoI C'CATG_G
Cuts at: 1217 1697 1217
Size: 480 4550
NgoMIV G'CCGG_C
Cuts at: 1724 3209 1724
Size: 1485 3545
NsiI A_TGCA'T
Cuts at: 1918 1918
Size: 5030
NspI r_CATG'y
Cuts at: 1150 2083 2583 3490 1150
Size: 933 500 907 2690
Fragments arranged by size:
2690 933 907 500
PasI CC'CwG_GG
Cuts at: 764 764
Size: 5030
PciI A'CATG_T
Cuts at: 3486 3486
Size: 5030
PfoI T'CCnGG_A
Cuts at: 584 1874 584
Size: 1290 3740
PmeI GTTT'AAAC
Cuts at: 2029 2029
Size: 5030
PmlI CAC'GTG
Cuts at: 577 577
Size: 5030
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4169 4201 4169
Size: 32 4998
PpuMI rG'GwC_Cy
Cuts at: 1086 1086
Size: 5030
PspOMI G'GGCC_C
Cuts at: 902 902
Size: 5030
PstI C_TGCA'G
Cuts at: 656 1094 1745 2077 2762 656
Size: 438 651 332 685 2924
Fragments arranged by size:
2924 685 651 438 332
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5030
PvuII CAG'CTG
Cuts at: 390 1533 2455 2815 390
Size: 1143 922 360 2605
Fragments arranged by size:
2605 1143 922 360
RsrII CG'GwC_CG
Cuts at: 3225 3225
Size: 5030
SacI G_AGCT'C
Cuts at: 2044 2044
Size: 5030
SalI G'TCGA_C
Cuts at: 2067 2067
Size: 5030
SapI GCTCTTCn'nnn_
Cuts at: 3053 3263 3053
Size: 210 4820
SbfI CC_TGCA'GG
Cuts at: 2077 2077
Size: 5030
ScaI AGT'ACT
Cuts at: 4547 4547
Size: 5030
SfcI C'TryA_G
Cuts at: 33 652 1090 1741 2073 2758 3751 3942 33
Size: 619 438 651 332 685 993 191 1121
Fragments arranged by size:
1121 993 685 651 619 438 332 191
SfoI GGC'GCC
Cuts at: 2462 2710 2462
Size: 248 4782
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5030
SmaI CCC'GGG
Cuts at: 2052 2052
Size: 5030
SmlI C'TyrA_G
Cuts at: 820 1491 2185 3100 3592 3854 820
Size: 671 694 915 492 262 1996
Fragments arranged by size:
1996 915 694 671 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5030
SphI G_CATG'C
Cuts at: 2083 2583 2083
Size: 500 4530
StuI AGG'CCT
Cuts at: 715 1306 1483 1844 715
Size: 591 177 361 3901
Fragments arranged by size:
3901 591 361 177
StyI C'CwwG_G
Cuts at: 475 1217 1697 2164 2518 475
Size: 742 480 467 354 2987
Fragments arranged by size:
2987 742 480 467 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 656 880 989 2613 2746 3205 3414 656
Size: 224 109 1624 133 459 209 2272
Fragments arranged by size:
2272 1624 459 224 209 133 109
TatI w'GTAC_w
Cuts at: 338 738 4545 338
Size: 400 3807 823
Fragments arranged by size:
3807 823 400
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 566 598 1196 1228 4547 4579 566
Size: 32 598 32 3319 32 1017
Fragments arranged by size:
3319 1017 598 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2827 2827
Size: 5030
XbaI T'CTAG_A
Cuts at: 2061 2061
Size: 5030
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 279 506 1903 2525 279
Size: 227 1397 622 2784
Fragments arranged by size:
2784 1397 622 227
XmaI C'CCGG_G
Cuts at: 2050 2050
Size: 5030
XmnI GAAnn'nnTTC
Cuts at: 1063 1334 1063
Size: 271 4759
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PasI PciI PfoI PmeI PmlI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BmgBI BmtI BsiWI BsmI BsmBI BssHII
BstEII BstZ17I ClaI CspCI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI PacI PflMI
PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI ZraI