Restriction map for
pF1KE0327
(Circular) (Six-base) MAPSORT of: pF1KE0327.seq Check: 9075 from: 1 to: 4967
pF1KE0327 4967 bp
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AccI GT'mk_AC
Cuts at: 1891 2005 1891
Size: 114 4853
Acc65I G'GTAC_C
Cuts at: 1983 1983
Size: 4967
AclI AA'CG_TT
Cuts at: 4807 4807
Size: 4967
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 296 1261 1573 2792 3224 3971 4239 157
Size: 139 965 312 1219 432 747 268 885
Fragments arranged by size:
1219 965 885 747 432 312 268 139
AflIII A'CryG_T
Cuts at: 2319 3423 4400 2319
Size: 1104 977 2886
Fragments arranged by size:
2886 1104 977
AgeI A'CCGG_T
Cuts at: 3355 3355
Size: 4967
AlwNI CAG_nnn'CTG
Cuts at: 135 2272 3839 135
Size: 2137 1567 1263
Fragments arranged by size:
2137 1567 1263
ApaI G_GGCC'C
Cuts at: 1445 1445
Size: 4967
ApaLI G'TGCA_C
Cuts at: 3737 3737
Size: 4967
ApoI r'AATT_y
Cuts at: 494 1044 1131 1462 1971 4320 4576 494
Size: 550 87 331 509 2349 256 885
Fragments arranged by size:
2349 885 550 509 331 256 87
AseI AT'TA_AT
Cuts at: 20 1031 20
Size: 1011 3956
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4967
AvaI C'yCGr_G
Cuts at: 1766 1987 1766
Size: 221 4746
BamHI G'GATC_C
Cuts at: 1992 1992
Size: 4967
BanI G'GyrC_C
Cuts at: 270 897 1983 2397 2645 2680 270
Size: 627 1086 414 248 35 2557
Fragments arranged by size:
2557 1086 627 414 248 35
BanII G_rGCy'C
Cuts at: 126 1397 1445 1981 3011 126
Size: 1271 48 536 1030 2082
Fragments arranged by size:
2082 1271 1030 536 48
BbeI G_GCGC'C
Cuts at: 2401 2649 2401
Size: 248 4719
BbsI GAAGACnn'nnnn_
Cuts at: 532 1374 532
Size: 842 4125
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 598 632 598
Size: 34 4933
BciVI GTATCCnnnnn_n'
Cuts at: 156 1244 1466 2865 3626 4967 156
Size: 1088 222 1399 761 1341 156
Fragments arranged by size:
1399 1341 1088 761 222 156
BclI T'GATC_A
Cuts at: 2487 2487
Size: 4967
BglI GCCn_nnn'nGGC
Cuts at: 140 140
Size: 4967
BglII A'GATC_T
Cuts at: 1840 2482 4519 1840
Size: 642 2037 2288
Fragments arranged by size:
2288 2037 642
BlpI GC'TnA_GC
Cuts at: 2079 2079
Size: 4967
Bme1580I G_kGCm'C
Cuts at: 273 1445 2592 2685 3741 273
Size: 1172 1147 93 1056 1499
Fragments arranged by size:
1499 1172 1147 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 378 2179 2260 2595 4739 378
Size: 1801 81 335 2144 606
Fragments arranged by size:
2144 1801 606 335 81
BmtI G_CTAG'C
Cuts at: 631 631
Size: 4967
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3334 3334
Size: 4967
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1357 1357
Size: 4967
Bpu10I CC'TnA_GC
Cuts at: 331 331
Size: 4967
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2143 3022 3514 3812 2143
Size: 879 492 298 3298
Fragments arranged by size:
3298 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4671 4671
Size: 4967
BsaAI yAC'GTr
Cuts at: 612 1902 2320 612
Size: 1290 418 3259
Fragments arranged by size:
3259 1290 418
BsaBI GATnn'nnATC
Cuts at: 6 1454 2481 4518 6
Size: 1448 1027 2037 455
Fragments arranged by size:
2037 1448 1027 455
BsaHI Gr'CG_yC
Cuts at: 2398 2646 3348 4844 2398
Size: 248 702 1496 2521
Fragments arranged by size:
2521 1496 702 248
BsaWI w'CCGG_w
Cuts at: 230 2156 2677 3355 3629 3776 4187 230
Size: 1926 521 678 274 147 411 1010
Fragments arranged by size:
1926 1010 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1146 1176 1146
Size: 30 4937
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 971 1225 971
Size: 254 4713
BseYI C'CCAG_C
Cuts at: 198 426 756 769 1555 2392 3150 3727 4223
Size: 228 330 13 786 837 758 577 496
Cuts at: 4223 198
Size: 942
Fragments arranged by size:
942 837 786 758 577 496 330 228
13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 818 1256 818
Size: 438 4529
BsiEI CG_ry'CG
Cuts at: 104 2509 2555 3763 104
Size: 2405 46 1208 1308
Fragments arranged by size:
2405 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 230 1343 1981 2759 2949 3741 230
Size: 1113 638 778 190 792 1456
Fragments arranged by size:
1456 1113 792 778 638 190
BsmI GAATG_Cn'
Cuts at: 673 1861 673
Size: 1188 3779
Bsp1286I G_dGCh'C
Cuts at: 126 230 273 1343 1397 1445 1981 2592 2685
Size: 104 43 1070 54 48 536 611 93
Cuts at: 2685 2759 2949 3011 3741 126
Size: 74 190 62 730 1352
Fragments arranged by size:
1352 1070 730 611 536 190 104 93
74 62 54 48 43
BspEI T'CCGG_A
Cuts at: 4187 4187
Size: 4967
BspHI T'CATG_A
Cuts at: 836 4143 836
Size: 3307 1660
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1115 2017 2231 2533 2914 1115
Size: 902 214 302 381 3168
Fragments arranged by size:
3168 902 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3259 3313 4797 4962 50
Size: 3209 54 1484 165 55
Fragments arranged by size:
3209 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 746 815 2879 746
Size: 69 2064 2834
Fragments arranged by size:
2834 2064 69
BsrFI r'CCGG_y
Cuts at: 2965 3146 3355 2965
Size: 181 209 4577
Fragments arranged by size:
4577 209 181
BsrGI T'GTAC_A
Cuts at: 698 698
Size: 4967
BssSI C'ACGA_G
Cuts at: 3238 3596 3238
Size: 358 4609
BstAPI GCAn_nnn'nTGC
Cuts at: 487 659 2344 487
Size: 172 1685 3110
Fragments arranged by size:
3110 1685 172
BstBI TT'CG_AA
Cuts at: 3328 3328
Size: 4967
BstYI r'GATC_y
Cuts at: 1 280 1840 1992 2459 2482 2817 3063 4075
Size: 279 1560 152 467 23 335 246 1012
Cuts at: 4075 4161 4173 4519 1
Size: 86 12 346 449
Fragments arranged by size:
1560 1012 467 449 346 335 279 246
152 86 23 12
BstZ17I GTA'TAC
Cuts at: 1892 1892
Size: 4967
BtgI C'CryG_G
Cuts at: 107 107
Size: 4967
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 549 2917 3098 3261 549
Size: 2368 181 163 2255
Fragments arranged by size:
2368 2255 181 163
BtsI GCAGTG_nn'
Cuts at: 162 2210 162
Size: 2048 2919
ClaI AT'CG_AT
Cuts at: 1548 1548
Size: 4967
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1129 1164 1641 1676 1129
Size: 35 477 35 4420
Fragments arranged by size:
4420 477 35 35
DraI TTT'AAA
Cuts at: 1966 1966
Size: 4967
DraIII CAC_nnn'GTG
Cuts at: 1317 1317
Size: 4967
DrdI GACnn_nn'nnGTC
Cuts at: 2673 3531 2673
Size: 858 4109
EaeI y'GGCC_r
Cuts at: 472 787 1649 2552 2726 3117 3144 4489 472
Size: 315 862 903 174 391 27 1345 950
Fragments arranged by size:
1345 950 903 862 391 315 174 27
EagI C'GGCC_G
Cuts at: 2552 2552
Size: 4967
EarI CTCTTCn'nnn_
Cuts at: 847 1688 2990 3200 847
Size: 841 1302 210 2614
Fragments arranged by size:
2614 1302 841 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3175 3485 3631 4777 3175
Size: 310 146 1146 3365
Fragments arranged by size:
3365 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1979 1979
Size: 4967
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 296 1261 1357 1573 2792 3224 3971 4239
Size: 139 965 96 216 1219 432 747 268
Cuts at: 4239 157
Size: 885
Fragments arranged by size:
1219 965 885 747 432 268 216 139
96
EcoO109I rG'GnC_Cy
Cuts at: 1442 1642 2106 1442
Size: 200 464 4303
Fragments arranged by size:
4303 464 200
EcoRI G'AATT_C
Cuts at: 494 1971 494
Size: 1477 3490
EcoRV GAT'ATC
Cuts at: 1330 1435 1716 1330
Size: 105 281 4581
Fragments arranged by size:
4581 281 105
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 876 2986 876
Size: 2110 2857
FspI TGC'GCA
Cuts at: 2748 2748
Size: 4967
HaeII r_GCGC'y
Cuts at: 135 1259 2401 2649 3671 4650 135
Size: 1124 1142 248 1022 979 452
Fragments arranged by size:
1142 1124 1022 979 452 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2809 2839 2841 2871 2809
Size: 30 2 30 4905
Fragments arranged by size:
4905 30 30 2
HincII GTy'rAC
Cuts at: 2006 2006
Size: 4967
KasI G'GCGC_C
Cuts at: 2397 2645 2397
Size: 248 4719
KpnI G_GTAC'C
Cuts at: 1987 1987
Size: 4967
MfeI C'AATT_G
Cuts at: 1578 1578
Size: 4967
MluI A'CGCG_T
Cuts at: 4400 4400
Size: 4967
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3638 3822 3638
Size: 184 4783
MscI TGG'CCA
Cuts at: 474 789 1651 2728 474
Size: 315 862 1077 2713
Fragments arranged by size:
2713 1077 862 315
MslI CAynn'nnrTG
Cuts at: 652 751 1242 2956 3083 652
Size: 99 491 1714 127 2536
Fragments arranged by size:
2536 1714 491 127 99
MspA1I CmG'CkG
Cuts at: 146 478 769 2078 2392 2752 3765 4010 146
Size: 332 291 1309 314 360 1013 245 1103
Fragments arranged by size:
1309 1103 1013 360 332 314 291 245
NaeI GCC'GGC
Cuts at: 3148 3148
Size: 4967
NarI GG'CG_CC
Cuts at: 2398 2646 2398
Size: 248 4719
NcoI C'CATG_G
Cuts at: 107 107
Size: 4967
NdeI CA'TA_TG
Cuts at: 432 432
Size: 4967
NgoMIV G'CCGG_C
Cuts at: 3146 3146
Size: 4967
NheI G'CTAG_C
Cuts at: 627 627
Size: 4967
NspI r_CATG'y
Cuts at: 657 2020 2520 3427 657
Size: 1363 500 907 2197
Fragments arranged by size:
2197 1363 907 500
PacI TTA_AT'TAA
Cuts at: 1031 1031
Size: 4967
PciI A'CATG_T
Cuts at: 3423 3423
Size: 4967
PmeI GTTT'AAAC
Cuts at: 1966 1966
Size: 4967
PmlI CAC'GTG
Cuts at: 612 612
Size: 4967
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4106 4138 4106
Size: 32 4935
PpuMI rG'GwC_Cy
Cuts at: 1642 1642
Size: 4967
PsiI TTA'TAA
Cuts at: 1183 1183
Size: 4967
PspOMI G'GGCC_C
Cuts at: 1441 1441
Size: 4967
PstI C_TGCA'G
Cuts at: 1115 2014 2699 1115
Size: 899 685 3383
Fragments arranged by size:
3383 899 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4967
PvuII CAG'CTG
Cuts at: 478 769 2392 2752 478
Size: 291 1623 360 2693
Fragments arranged by size:
2693 1623 360 291
RsrII CG'GwC_CG
Cuts at: 233 3162 233
Size: 2929 2038
SacI G_AGCT'C
Cuts at: 1981 1981
Size: 4967
SalI G'TCGA_C
Cuts at: 2004 2004
Size: 4967
SapI GCTCTTCn'nnn_
Cuts at: 1688 2990 3200 1688
Size: 1302 210 3455
Fragments arranged by size:
3455 1302 210
SbfI CC_TGCA'GG
Cuts at: 2014 2014
Size: 4967
ScaI AGT'ACT
Cuts at: 4484 4484
Size: 4967
SfcI C'TryA_G
Cuts at: 33 1111 1478 1815 2010 2695 3688 3879 33
Size: 1078 367 337 195 685 993 191 1121
Fragments arranged by size:
1121 1078 993 685 367 337 195 191
SfoI GGC'GCC
Cuts at: 2399 2647 2399
Size: 248 4719
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4967
SmaI CCC'GGG
Cuts at: 1768 1989 1768
Size: 221 4746
SmlI C'TyrA_G
Cuts at: 2122 3037 3529 3791 2122
Size: 915 492 262 3298
Fragments arranged by size:
3298 915 492 262
SnaBI TAC'GTA
Cuts at: 1902 1902
Size: 4967
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4967
SphI G_CATG'C
Cuts at: 2020 2520 2020
Size: 500 4467
SspI AAT'ATT
Cuts at: 421 1780 421
Size: 1359 3608
StyI C'CwwG_G
Cuts at: 107 1157 2101 2455 107
Size: 1050 944 354 2619
Fragments arranged by size:
2619 1050 944 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 250 2550 2683 3142 3351 250
Size: 2300 133 459 209 1866
Fragments arranged by size:
2300 1866 459 209 133
TatI w'GTAC_w
Cuts at: 698 1292 4482 698
Size: 594 3190 1183
Fragments arranged by size:
3190 1183 594
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1938 1970 4484 4516 1938
Size: 32 2514 32 2389
Fragments arranged by size:
2514 2389 32 32
Tth111I GACn'n_nGTC
Cuts at: 2764 2764
Size: 4967
XbaI T'CTAG_A
Cuts at: 1689 1837 1998 1689
Size: 148 161 4658
Fragments arranged by size:
4658 161 148
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 753 1172 2462 753
Size: 419 1290 3258
Fragments arranged by size:
3258 1290 419
XmaI C'CCGG_G
Cuts at: 1766 1987 1766
Size: 221 4746
XmnI GAAnn'nnTTC
Cuts at: 404 856 404
Size: 452 4515
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NspI PacI PciI PmeI
PmlI PpiI PpuMI PsiI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbvCI BfrBI BmgBI BsiWI BsmBI
BssHII BstEII BstXI Bsu36I EcoNI FseI FspAI HindIII
HpaI NotI NruI NsiI PasI PflMI PfoI PshAI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SrfI
StuI SwaI XhoI ZraI