Restriction map for
pF1KE0329
(Circular) (Six-base) MAPSORT of: pF1KE0329.seq Check: 4922 from: 1 to: 4637
pF1KE0329 4637 bp
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 178 178
Size: 4637
AccI GT'mk_AC
Cuts at: 205 502 1675 205
Size: 297 1173 3167
Fragments arranged by size:
3167 1173 297
Acc65I G'GTAC_C
Cuts at: 1653 1653
Size: 4637
AclI AA'CG_TT
Cuts at: 4477 4477
Size: 4637
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1418 2462 2894 3641 3909 1418
Size: 1044 432 747 268 2146
Fragments arranged by size:
2146 1044 747 432 268
AfeI AGC'GCT
Cuts at: 1272 1272
Size: 4637
AflIII A'CryG_T
Cuts at: 1989 3093 4070 1989
Size: 1104 977 2556
Fragments arranged by size:
2556 1104 977
AgeI A'CCGG_T
Cuts at: 3025 3025
Size: 4637
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1169 1169
Size: 4637
AlwNI CAG_nnn'CTG
Cuts at: 607 1942 3509 607
Size: 1335 1567 1735
Fragments arranged by size:
1735 1567 1335
ApaLI G'TGCA_C
Cuts at: 3407 3407
Size: 4637
ApoI r'AATT_y
Cuts at: 368 422 1070 1216 1556 1614 1641 3990 4246
Size: 54 648 146 340 58 27 2349 256
Cuts at: 4246 368
Size: 759
Fragments arranged by size:
2349 759 648 340 256 146 58 54
27
AseI AT'TA_AT
Cuts at: 20 1390 20
Size: 1370 3267
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4637
AvaI C'yCGr_G
Cuts at: 899 1657 899
Size: 758 3879
BamHI G'GATC_C
Cuts at: 1662 1662
Size: 4637
BanI G'GyrC_C
Cuts at: 124 1653 2067 2315 2350 124
Size: 1529 414 248 35 2411
Fragments arranged by size:
2411 1529 414 248 35
BanII G_rGCy'C
Cuts at: 1651 2681 1651
Size: 1030 3607
BbeI G_GCGC'C
Cuts at: 128 2071 2319 128
Size: 1943 248 2446
Fragments arranged by size:
2446 1943 248
BbsI GAAGACnn'nnnn_
Cuts at: 162 1075 1305 162
Size: 913 230 3494
Fragments arranged by size:
3494 913 230
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4603
BciVI GTATCCnnnnn_n'
Cuts at: 2535 3296 4637 2535
Size: 761 1341 2535
Fragments arranged by size:
2535 1341 761
BclI T'GATC_A
Cuts at: 2157 2157
Size: 4637
BfrBI ATG'CAT
Cuts at: 444 444
Size: 4637
BglI GCCn_nnn'nGGC
Cuts at: 133 133
Size: 4637
BglII A'GATC_T
Cuts at: 2152 4189 2152
Size: 2037 2600
BlpI GC'TnA_GC
Cuts at: 1749 1749
Size: 4637
Bme1580I G_kGCm'C
Cuts at: 2262 2355 3411 2262
Size: 93 1056 3488
Fragments arranged by size:
3488 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1849 1930 2265 4409 1849
Size: 81 335 2144 2077
Fragments arranged by size:
2144 2077 335 81
BmtI G_CTAG'C
Cuts at: 1006 1006
Size: 4637
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 965 3004 965
Size: 2039 2598
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 880 1404 880
Size: 524 4113
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1559 1813 2692 3184 3482 1559
Size: 254 879 492 298 2714
Fragments arranged by size:
2714 879 492 298 254
BsaI GGTCTCn'nnnn_
Cuts at: 669 4341 669
Size: 3672 965
BsaAI yAC'GTr
Cuts at: 1990 1990
Size: 4637
BsaBI GATnn'nnATC
Cuts at: 6 2151 4188 6
Size: 2145 2037 455
Fragments arranged by size:
2145 2037 455
BsaHI Gr'CG_yC
Cuts at: 125 2068 2316 3018 4514 125
Size: 1943 248 702 1496 248
Fragments arranged by size:
1943 1496 702 248 248
BsaWI w'CCGG_w
Cuts at: 1826 2347 3025 3299 3446 3857 1826
Size: 521 678 274 147 411 2606
Fragments arranged by size:
2606 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 632 804 1118 632
Size: 172 314 4151
Fragments arranged by size:
4151 314 172
BseYI C'CCAG_C
Cuts at: 2062 2820 3397 3893 2062
Size: 758 577 496 2806
Fragments arranged by size:
2806 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 647 956 1139 647
Size: 309 183 4145
Fragments arranged by size:
4145 309 183
BsiEI CG_ry'CG
Cuts at: 104 137 2179 2225 3433 104
Size: 33 2042 46 1208 1308
Fragments arranged by size:
2042 1308 1208 46 33
BsiHKAI G_wGCw'C
Cuts at: 1651 2429 2619 3411 1651
Size: 778 190 792 2877
Fragments arranged by size:
2877 792 778 190
BsmI GAATG_Cn'
Cuts at: 860 860
Size: 4637
BsmBI CGTCTCn'nnnn_
Cuts at: 1526 1526
Size: 4637
Bsp1286I G_dGCh'C
Cuts at: 1651 2262 2355 2429 2619 2681 3411 1651
Size: 611 93 74 190 62 730 2877
Fragments arranged by size:
2877 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3857 3857
Size: 4637
BspHI T'CATG_A
Cuts at: 3813 3813
Size: 4637
BspMI ACCTGCnnnn'nnnn_
Cuts at: 178 687 1687 1901 2203 2584 178
Size: 509 1000 214 302 381 2231
Fragments arranged by size:
2231 1000 509 381 302 214
BsrBI CCG'CTC
Cuts at: 50 161 2929 2983 4467 4632 50
Size: 111 2768 54 1484 165 55
Fragments arranged by size:
2768 1484 165 111 55 54
BsrDI GCAATG_nn'
Cuts at: 2549 2549
Size: 4637
BsrFI r'CCGG_y
Cuts at: 2635 2816 3025 2635
Size: 181 209 4247
Fragments arranged by size:
4247 209 181
BsrGI T'GTAC_A
Cuts at: 1317 1317
Size: 4637
BssSI C'ACGA_G
Cuts at: 2908 3266 2908
Size: 358 4279
BstAPI GCAn_nnn'nTGC
Cuts at: 634 2014 634
Size: 1380 3257
BstBI TT'CG_AA
Cuts at: 2998 2998
Size: 4637
BstYI r'GATC_y
Cuts at: 1 1662 2129 2152 2487 2733 3745 3831 3843
Size: 1661 467 23 335 246 1012 86 12
Cuts at: 3843 4189 1
Size: 346 449
Fragments arranged by size:
1661 1012 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 126 2587 2768 2931 126
Size: 2461 181 163 1832
Fragments arranged by size:
2461 1832 181 163
BtsI GCAGTG_nn'
Cuts at: 1880 1880
Size: 4637
ClaI AT'CG_AT
Cuts at: 281 281
Size: 4637
DraI TTT'AAA
Cuts at: 1542 1636 1542
Size: 94 4543
DrdI GACnn_nn'nnGTC
Cuts at: 2343 3201 2343
Size: 858 3779
EaeI y'GGCC_r
Cuts at: 134 149 2222 2396 2787 2814 4159 134
Size: 15 2073 174 391 27 1345 612
Fragments arranged by size:
2073 1345 612 391 174 27 15
EagI C'GGCC_G
Cuts at: 134 2222 134
Size: 2088 2549
EarI CTCTTCn'nnn_
Cuts at: 734 1130 1329 2660 2870 734
Size: 396 199 1331 210 2501
Fragments arranged by size:
2501 1331 396 210 199
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1406 2845 3155 3301 4447 1406
Size: 1439 310 146 1146 1596
Fragments arranged by size:
1596 1439 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1649 1649
Size: 4637
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 880 1404 1418 2462 2894 3641 3909 880
Size: 524 14 1044 432 747 268 1608
Fragments arranged by size:
1608 1044 747 524 432 268 14
EcoO109I rG'GnC_Cy
Cuts at: 687 1776 687
Size: 1089 3548
EcoRI G'AATT_C
Cuts at: 368 422 1070 1641 368
Size: 54 648 571 3364
Fragments arranged by size:
3364 648 571 54
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2656 2656
Size: 4637
FspI TGC'GCA
Cuts at: 2418 2418
Size: 4637
HaeII r_GCGC'y
Cuts at: 128 1274 2071 2319 3341 4320 128
Size: 1146 797 248 1022 979 445
Fragments arranged by size:
1146 1022 979 797 445 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 187 219 583 615 2479 2509 2511 2541 187
Size: 32 364 32 1864 30 2 30 2283
Fragments arranged by size:
2283 1864 364 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1676 1676
Size: 4637
KasI G'GCGC_C
Cuts at: 124 2067 2315 124
Size: 1943 248 2446
Fragments arranged by size:
2446 1943 248
KpnI G_GTAC'C
Cuts at: 1657 1657
Size: 4637
MluI A'CGCG_T
Cuts at: 4070 4070
Size: 4637
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 459 794 3308 3492 459
Size: 335 2514 184 1604
Fragments arranged by size:
2514 1604 335 184
MscI TGG'CCA
Cuts at: 2398 2398
Size: 4637
MslI CAynn'nnrTG
Cuts at: 700 2626 2753 700
Size: 1926 127 2584
Fragments arranged by size:
2584 1926 127
MspA1I CmG'CkG
Cuts at: 1331 1748 2062 2422 3435 3680 1331
Size: 417 314 360 1013 245 2288
Fragments arranged by size:
2288 1013 417 360 314 245
NaeI GCC'GGC
Cuts at: 2818 2818
Size: 4637
NarI GG'CG_CC
Cuts at: 125 2068 2316 125
Size: 1943 248 2446
Fragments arranged by size:
2446 1943 248
NgoMIV G'CCGG_C
Cuts at: 2816 2816
Size: 4637
NheI G'CTAG_C
Cuts at: 1002 1002
Size: 4637
NruI TCG'CGA
Cuts at: 145 145
Size: 4637
NsiI A_TGCA'T
Cuts at: 446 446
Size: 4637
NspI r_CATG'y
Cuts at: 1690 2190 3097 1690
Size: 500 907 3230
Fragments arranged by size:
3230 907 500
PciI A'CATG_T
Cuts at: 3093 3093
Size: 4637
PflMI CCAn_nnn'nTGG
Cuts at: 113 113
Size: 4637
PmeI GTTT'AAAC
Cuts at: 1636 1636
Size: 4637
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3776 3808 3776
Size: 32 4605
PpuMI rG'GwC_Cy
Cuts at: 687 687
Size: 4637
PsiI TTA'TAA
Cuts at: 821 1235 821
Size: 414 4223
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 320 352 320
Size: 32 4605
PstI C_TGCA'G
Cuts at: 405 684 1684 2369 405
Size: 279 1000 685 2673
Fragments arranged by size:
2673 1000 685 279
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4637
PvuII CAG'CTG
Cuts at: 2062 2422 2062
Size: 360 4277
RsrII CG'GwC_CG
Cuts at: 2832 2832
Size: 4637
SacI G_AGCT'C
Cuts at: 1651 1651
Size: 4637
SalI G'TCGA_C
Cuts at: 1674 1674
Size: 4637
SapI GCTCTTCn'nnn_
Cuts at: 2660 2870 2660
Size: 210 4427
SbfI CC_TGCA'GG
Cuts at: 1684 1684
Size: 4637
ScaI AGT'ACT
Cuts at: 4154 4154
Size: 4637
SfcI C'TryA_G
Cuts at: 33 304 326 401 680 1680 2365 3358 3549
Size: 271 22 75 279 1000 685 993 191
Cuts at: 3549 33
Size: 1121
Fragments arranged by size:
1121 1000 993 685 279 271 191 75
22
SfoI GGC'GCC
Cuts at: 126 2069 2317 126
Size: 1943 248 2446
Fragments arranged by size:
2446 1943 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4637
SmaI CCC'GGG
Cuts at: 1659 1659
Size: 4637
SmlI C'TyrA_G
Cuts at: 899 1574 1792 2707 3199 3461 899
Size: 675 218 915 492 262 2075
Fragments arranged by size:
2075 915 675 492 262 218
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4637
SphI G_CATG'C
Cuts at: 1690 2190 1690
Size: 500 4137
StyI C'CwwG_G
Cuts at: 374 1771 2125 374
Size: 1397 354 2886
Fragments arranged by size:
2886 1397 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 597 2220 2353 2812 3021 597
Size: 1623 133 459 209 2213
Fragments arranged by size:
2213 1623 459 209 133
TatI w'GTAC_w
Cuts at: 1317 4152 1317
Size: 2835 1802
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4154 4186 4154
Size: 32 4605
Tth111I GACn'n_nGTC
Cuts at: 2434 2434
Size: 4637
XbaI T'CTAG_A
Cuts at: 1131 1668 1131
Size: 537 4100
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1626 2132 1626
Size: 506 4131
XhoI C'TCGA_G
Cuts at: 899 899
Size: 4637
XmaI C'CCGG_G
Cuts at: 1657 1657
Size: 4637
XmnI GAAnn'nnTTC
Cuts at: 396 396
Size: 4637
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI BtgZI BtsI ClaI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NheI NruI NsiI
NspI PciI PflMI PmeI PpiI PpuMI PsiI PsrI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AloI ApaI AscI AvrII
BaeI BbvCI BmgBI Bpu10I BsaXI BsiWI BssHII BstEII
BstXI BstZ17I Bsu36I BtgI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NotI
PacI PasI PfoI PmlI PshAI PspOMI PspXI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI ZraI