Restriction map for
pF1KE0335
(Circular) (Six-base) MAPSORT of: pF1KE0335.seq Check: 7983 from: 1 to: 5855
pF1KE0335 5855 bp
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AccI GT'mk_AC
Cuts at: 530 2893 530
Size: 2363 3492
Acc65I G'GTAC_C
Cuts at: 2003 2871 2003
Size: 868 4987
AclI AA'CG_TT
Cuts at: 5695 5695
Size: 5855
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 517 563 2516 2688 3680 4112 4859 5127 517
Size: 46 1953 172 992 432 747 268 1245
Fragments arranged by size:
1953 1245 992 747 432 268 172 46
AfeI AGC'GCT
Cuts at: 2125 2125
Size: 5855
AflIII A'CryG_T
Cuts at: 1816 2169 3207 4311 5288 1816
Size: 353 1038 1104 977 2383
Fragments arranged by size:
2383 1104 1038 977 353
AgeI A'CCGG_T
Cuts at: 4243 4243
Size: 5855
AhdI GACnn_n'nnGTC
Cuts at: 537 537
Size: 5855
AleI CACnn'nnGTG
Cuts at: 1069 1069
Size: 5855
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1087 1706 1087
Size: 619 5236
AlwNI CAG_nnn'CTG
Cuts at: 176 1000 1039 3160 4727 176
Size: 824 39 2121 1567 1304
Fragments arranged by size:
2121 1567 1304 824 39
ApaI G_GGCC'C
Cuts at: 1033 1033
Size: 5855
ApaLI G'TGCA_C
Cuts at: 1072 4625 1072
Size: 3553 2302
ApoI r'AATT_y
Cuts at: 326 567 700 709 1895 2859 5208 5464 326
Size: 241 133 9 1186 964 2349 256 717
Fragments arranged by size:
2349 1186 964 717 256 241 133 9
AseI AT'TA_AT
Cuts at: 20 20
Size: 5855
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5855
AvaI C'yCGr_G
Cuts at: 2875 2875
Size: 5855
BamHI G'GATC_C
Cuts at: 788 2880 788
Size: 2092 3763
BanI G'GyrC_C
Cuts at: 1849 2003 2871 3285 3533 3568 1849
Size: 154 868 414 248 35 4136
Fragments arranged by size:
4136 868 414 248 154 35
BanII G_rGCy'C
Cuts at: 1033 1727 2072 2869 3899 1033
Size: 694 345 797 1030 2989
Fragments arranged by size:
2989 1030 797 694 345
BbeI G_GCGC'C
Cuts at: 3289 3537 3289
Size: 248 5607
BbsI GAAGACnn'nnnn_
Cuts at: 275 1131 1508 275
Size: 856 377 4622
Fragments arranged by size:
4622 856 377
BciVI GTATCCnnnnn_n'
Cuts at: 865 1192 3753 4514 5855 865
Size: 327 2561 761 1341 865
Fragments arranged by size:
2561 1341 865 761 327
BclI T'GATC_A
Cuts at: 2630 3375 2630
Size: 745 5110
BglII A'GATC_T
Cuts at: 678 1057 2687 3370 5407 678
Size: 379 1630 683 2037 1126
Fragments arranged by size:
2037 1630 1126 683 379
BlpI GC'TnA_GC
Cuts at: 2967 2967
Size: 5855
Bme1580I G_kGCm'C
Cuts at: 1033 1076 3480 3573 4629 1033
Size: 43 2404 93 1056 2259
Fragments arranged by size:
2404 2259 1056 93 43
BmrI ACTGGGnnnn_n'
Cuts at: 943 1027 1940 2389 2841 3067 3148 3483 5627
Size: 84 913 449 452 226 81 335 2144
Cuts at: 5627 943
Size: 1171
Fragments arranged by size:
2144 1171 913 452 449 335 226 84
81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2826 4222 2826
Size: 1396 4459
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2033 2821 2033
Size: 788 5067
Bpu10I CC'TnA_GC
Cuts at: 1381 2794 1381
Size: 1413 4442
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3031 3910 4402 4700 3031
Size: 879 492 298 4186
Fragments arranged by size:
4186 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 485 1534 1794 5559 485
Size: 1049 260 3765 781
Fragments arranged by size:
3765 1049 781 260
BsaAI yAC'GTr
Cuts at: 2172 2771 3208 2172
Size: 599 437 4819
Fragments arranged by size:
4819 599 437
BsaBI GATnn'nnATC
Cuts at: 6 3369 5406 6
Size: 3363 2037 455
Fragments arranged by size:
3363 2037 455
BsaHI Gr'CG_yC
Cuts at: 201 3286 3534 4236 5732 201
Size: 3085 248 702 1496 324
Fragments arranged by size:
3085 1496 702 324 248
BsaWI w'CCGG_w
Cuts at: 402 3044 3565 4243 4517 4664 5075 402
Size: 2642 521 678 274 147 411 1182
Fragments arranged by size:
2642 1182 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 902 932 902
Size: 30 5825
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 368 652 655 1524 368
Size: 284 3 869 4699
Fragments arranged by size:
4699 869 284 3
BseYI C'CCAG_C
Cuts at: 2256 2665 3280 4038 4615 5111 2256
Size: 409 615 758 577 496 3000
Fragments arranged by size:
3000 758 615 577 496 409
BsiEI CG_ry'CG
Cuts at: 104 3397 3443 4651 104
Size: 3293 46 1208 1308
Fragments arranged by size:
3293 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1076 1310 1718 1727 2869 3647 3837 4629 1076
Size: 234 408 9 1142 778 190 792 2302
Fragments arranged by size:
2302 1142 792 778 408 234 190 9
BsmI GAATG_Cn'
Cuts at: 969 1695 969
Size: 726 5129
Bsp1286I G_dGCh'C
Cuts at: 1033 1076 1310 1718 1727 2072 2869 3480 3573
Size: 43 234 408 9 345 797 611 93
Cuts at: 3573 3647 3837 3899 4629 1033
Size: 74 190 62 730 2259
Fragments arranged by size:
2259 797 730 611 408 345 234 190
93 74 62 43 9
BspEI T'CCGG_A
Cuts at: 402 5075 402
Size: 4673 1182
BspHI T'CATG_A
Cuts at: 2189 5031 2189
Size: 2842 3013
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2905 3119 3421 3802 2905
Size: 214 302 381 4958
Fragments arranged by size:
4958 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4147 4201 5685 5850 50
Size: 4097 54 1484 165 55
Fragments arranged by size:
4097 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2819 3767 2819
Size: 948 4907
BsrFI r'CCGG_y
Cuts at: 3853 4034 4243 3853
Size: 181 209 5465
Fragments arranged by size:
5465 209 181
BssSI C'ACGA_G
Cuts at: 4126 4484 4126
Size: 358 5497
BstAPI GCAn_nnn'nTGC
Cuts at: 1252 1377 1680 2595 3232 1252
Size: 125 303 915 637 3875
Fragments arranged by size:
3875 915 637 303 125
BstBI TT'CG_AA
Cuts at: 4216 4216
Size: 5855
BstXI CCAn_nnnn'nTGG
Cuts at: 1296 1296
Size: 5855
BstYI r'GATC_y
Cuts at: 1 678 788 1057 1609 1934 2390 2687 2880
Size: 677 110 269 552 325 456 297 193
Cuts at: 2880 3347 3370 3705 3951 4963 5049 5061 5407
Size: 467 23 335 246 1012 86 12 346
Cuts at: 5407 1
Size: 449
Fragments arranged by size:
1012 677 552 467 456 449 346 335
325 297 269 246 193 110 86 23
12
BtgI C'CryG_G
Cuts at: 107 2034 107
Size: 1927 3928
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3805 3986 4149 3805
Size: 181 163 5511
Fragments arranged by size:
5511 181 163
BtsI GCAGTG_nn'
Cuts at: 125 1253 3098 125
Size: 1128 1845 2882
Fragments arranged by size:
2882 1845 1128
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2289 2324 2289
Size: 35 5820
DraI TTT'AAA
Cuts at: 2854 2854
Size: 5855
DrdI GACnn_nn'nnGTC
Cuts at: 2836 3561 4419 2836
Size: 725 858 4272
Fragments arranged by size:
4272 858 725
EaeI y'GGCC_r
Cuts at: 1522 2037 2559 3440 3614 4005 4032 5377 1522
Size: 515 522 881 174 391 27 1345 2000
Fragments arranged by size:
2000 1345 881 522 515 391 174 27
EagI C'GGCC_G
Cuts at: 3440 3440
Size: 5855
EarI CTCTTCn'nnn_
Cuts at: 365 542 689 1630 3878 4088 365
Size: 177 147 941 2248 210 2132
Fragments arranged by size:
2248 2132 941 210 177 147
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4063 4373 4519 5665 4063
Size: 310 146 1146 4253
Fragments arranged by size:
4253 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1725 2867 1725
Size: 1142 4713
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 517 563 2033 2516 2688 2821 3680 4112 4859
Size: 46 1470 483 172 133 859 432 747
Cuts at: 4859 5127 517
Size: 268 1245
Fragments arranged by size:
1470 1245 859 747 483 432 268 172
133 46
EcoO109I rG'GnC_Cy
Cuts at: 1120 2994 1120
Size: 1874 3981
EcoRI G'AATT_C
Cuts at: 2859 2859
Size: 5855
EcoRV GAT'ATC
Cuts at: 720 720
Size: 5855
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 549 1344 3874 549
Size: 795 2530 2530
Fragments arranged by size:
2530 2530 795
FspI TGC'GCA
Cuts at: 3636 3636
Size: 5855
HaeII r_GCGC'y
Cuts at: 2127 3289 3537 4559 5538 2127
Size: 1162 248 1022 979 2444
Fragments arranged by size:
2444 1162 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 523 555 631 663 2099 2131 2346 2378 3697
Size: 32 76 32 1436 32 215 32 1319
Cuts at: 3697 3727 3729 3759 523
Size: 30 2 30 2619
Fragments arranged by size:
2619 1436 1319 215 76 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2894 2894
Size: 5855
HindIII A'AGCT_T
Cuts at: 1399 1399
Size: 5855
KasI G'GCGC_C
Cuts at: 3285 3533 3285
Size: 248 5607
KpnI G_GTAC'C
Cuts at: 2007 2875 2007
Size: 868 4987
MfeI C'AATT_G
Cuts at: 1707 1707
Size: 5855
MluI A'CGCG_T
Cuts at: 5288 5288
Size: 5855
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4526 4710 4526
Size: 184 5671
MscI TGG'CCA
Cuts at: 1524 2039 2561 3616 1524
Size: 515 522 1055 3763
Fragments arranged by size:
3763 1055 522 515
MslI CAynn'nnrTG
Cuts at: 782 1069 3844 3971 782
Size: 287 2775 127 2666
Fragments arranged by size:
2775 2666 287 127
MspA1I CmG'CkG
Cuts at: 1214 2966 3280 3640 4653 4898 1214
Size: 1752 314 360 1013 245 2171
Fragments arranged by size:
2171 1752 1013 360 314 245
NaeI GCC'GGC
Cuts at: 4036 4036
Size: 5855
NarI GG'CG_CC
Cuts at: 3286 3534 3286
Size: 248 5607
NcoI C'CATG_G
Cuts at: 107 107
Size: 5855
NgoMIV G'CCGG_C
Cuts at: 4034 4034
Size: 5855
NspI r_CATG'y
Cuts at: 802 2256 2908 3408 4315 802
Size: 1454 652 500 907 2342
Fragments arranged by size:
2342 1454 907 652 500
PasI CC'CwG_GG
Cuts at: 573 573
Size: 5855
PciI A'CATG_T
Cuts at: 4311 4311
Size: 5855
PflMI CCAn_nnn'nTGG
Cuts at: 933 2477 2831 933
Size: 1544 354 3957
Fragments arranged by size:
3957 1544 354
PfoI T'CCnGG_A
Cuts at: 2322 2322
Size: 5855
PmeI GTTT'AAAC
Cuts at: 2854 2854
Size: 5855
PmlI CAC'GTG
Cuts at: 2172 2172
Size: 5855
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4994 5026 4994
Size: 32 5823
PpuMI rG'GwC_Cy
Cuts at: 1120 1120
Size: 5855
PsiI TTA'TAA
Cuts at: 854 854
Size: 5855
PspOMI G'GGCC_C
Cuts at: 1029 1029
Size: 5855
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2140 2172 2140
Size: 32 5823
PstI C_TGCA'G
Cuts at: 1005 1767 2902 3587 1005
Size: 762 1135 685 3273
Fragments arranged by size:
3273 1135 762 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5855
PvuII CAG'CTG
Cuts at: 3280 3640 3280
Size: 360 5495
RsrII CG'GwC_CG
Cuts at: 4050 4050
Size: 5855
SacI G_AGCT'C
Cuts at: 1727 2869 1727
Size: 1142 4713
SalI G'TCGA_C
Cuts at: 2892 2892
Size: 5855
SapI GCTCTTCn'nnn_
Cuts at: 3878 4088 3878
Size: 210 5645
SbfI CC_TGCA'GG
Cuts at: 2902 2902
Size: 5855
ScaI AGT'ACT
Cuts at: 1150 5372 1150
Size: 4222 1633
SexAI A'CCwGG_T
Cuts at: 277 277
Size: 5855
SfcI C'TryA_G
Cuts at: 33 152 598 944 1001 1763 2459 2898 3583
Size: 119 446 346 57 762 696 439 685
Cuts at: 3583 4576 4767 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 762 696 685 446 439 346
191 119 57
SfoI GGC'GCC
Cuts at: 3287 3535 3287
Size: 248 5607
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5855
SmaI CCC'GGG
Cuts at: 2877 2877
Size: 5855
SmlI C'TyrA_G
Cuts at: 3010 3925 4417 4679 3010
Size: 915 492 262 4186
Fragments arranged by size:
4186 915 492 262
SnaBI TAC'GTA
Cuts at: 2771 2771
Size: 5855
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5855
SphI G_CATG'C
Cuts at: 2908 3408 2908
Size: 500 5355
StuI AGG'CCT
Cuts at: 2196 2196
Size: 5855
StyI C'CwwG_G
Cuts at: 107 1559 2402 2989 3343 107
Size: 1452 843 587 354 2619
Fragments arranged by size:
2619 1452 843 587 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2527 3438 3571 4030 4239 2527
Size: 911 133 459 209 4143
Fragments arranged by size:
4143 911 459 209 133
TatI w'GTAC_w
Cuts at: 1010 1148 2380 5370 1010
Size: 138 1232 2990 1495
Fragments arranged by size:
2990 1495 1232 138
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 698 730 2161 2193 2362 2394 5372 5404 698
Size: 32 1431 32 169 32 2978 32 1149
Fragments arranged by size:
2978 1431 1149 169 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3652 3652
Size: 5855
XbaI T'CTAG_A
Cuts at: 2886 2886
Size: 5855
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2673 2740 2796 3350 2673
Size: 67 56 554 5178
Fragments arranged by size:
5178 554 67 56
XmaI C'CCGG_G
Cuts at: 2875 2875
Size: 5855
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PsiI PspOMI PsrI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AloI AscI AvrII BaeI BbvCI
BcgI BfrBI BglI BmgBI BmtI BsgI BsiWI BsmBI
BsrGI BssHII BstEII BstZ17I Bsu36I ClaI DraIII EcoNI
FseI FspAI HpaI NdeI NheI NotI NruI NsiI
PacI PshAI PspXI SacII SanDI SfiI SgrAI SrfI
SspI SwaI XhoI XmnI ZraI