Restriction map for
pF1KE0336
(Circular) (Six-base) MAPSORT of: pF1KE0336.seq Check: 1141 from: 1 to: 4577
pF1KE0336 4577 bp
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1535 1535
Size: 4577
AatII G_ACGT'C
Cuts at: 1274 1274
Size: 4577
AccI GT'mk_AC
Cuts at: 959 1615 959
Size: 656 3921
Acc65I G'GTAC_C
Cuts at: 1593 1593
Size: 4577
AclI AA'CG_TT
Cuts at: 4417 4417
Size: 4577
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2402 2834 3581 3849 2402
Size: 432 747 268 3130
Fragments arranged by size:
3130 747 432 268
AflIII A'CryG_T
Cuts at: 1929 3033 4010 1929
Size: 1104 977 2496
Fragments arranged by size:
2496 1104 977
AgeI A'CCGG_T
Cuts at: 2965 2965
Size: 4577
AleI CACnn'nnGTG
Cuts at: 202 1483 202
Size: 1281 3296
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 847 879 847
Size: 32 4545
AlwNI CAG_nnn'CTG
Cuts at: 1088 1242 1340 1882 3449 1088
Size: 154 98 542 1567 2216
Fragments arranged by size:
2216 1567 542 154 98
ApaI G_GGCC'C
Cuts at: 618 618
Size: 4577
ApaLI G'TGCA_C
Cuts at: 3347 3347
Size: 4577
ApoI r'AATT_y
Cuts at: 1581 3930 4186 1581
Size: 2349 256 1972
Fragments arranged by size:
2349 1972 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4577
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4577
AvaI C'yCGr_G
Cuts at: 313 1597 313
Size: 1284 3293
BamHI G'GATC_C
Cuts at: 758 1602 758
Size: 844 3733
BanI G'GyrC_C
Cuts at: 1254 1593 2007 2255 2290 1254
Size: 339 414 248 35 3541
Fragments arranged by size:
3541 414 339 248 35
BanII G_rGCy'C
Cuts at: 618 735 1427 1591 2621 618
Size: 117 692 164 1030 2574
Fragments arranged by size:
2574 1030 692 164 117
BbeI G_GCGC'C
Cuts at: 2011 2259 2011
Size: 248 4329
BciVI GTATCCnnnnn_n'
Cuts at: 2475 3236 4577 2475
Size: 761 1341 2475
Fragments arranged by size:
2475 1341 761
BclI T'GATC_A
Cuts at: 2097 2097
Size: 4577
BglI GCCn_nnn'nGGC
Cuts at: 484 592 751 484
Size: 108 159 4310
Fragments arranged by size:
4310 159 108
BglII A'GATC_T
Cuts at: 2092 4129 2092
Size: 2037 2540
BlpI GC'TnA_GC
Cuts at: 1689 1689
Size: 4577
Bme1580I G_kGCm'C
Cuts at: 618 1259 2202 2295 3351 618
Size: 641 943 93 1056 1844
Fragments arranged by size:
1844 1056 943 641 93
BmgBI CAC'GTC
Cuts at: 1290 1290
Size: 4577
BmrI ACTGGGnnnn_n'
Cuts at: 879 1003 1789 1870 2205 4349 879
Size: 124 786 81 335 2144 1107
Fragments arranged by size:
2144 1107 786 335 124 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2944 2944
Size: 4577
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 283 324 1362 1570 283
Size: 41 1038 208 3290
Fragments arranged by size:
3290 1038 208 41
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 301 1753 2632 3124 3422 301
Size: 1452 879 492 298 1456
Fragments arranged by size:
1456 1452 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1145 4281 1145
Size: 3136 1441
BsaAI yAC'GTr
Cuts at: 357 1930 357
Size: 1573 3004
BsaBI GATnn'nnATC
Cuts at: 6 2091 4128 6
Size: 2085 2037 455
Fragments arranged by size:
2085 2037 455
BsaHI Gr'CG_yC
Cuts at: 1226 1271 2008 2256 2958 4454 1226
Size: 45 737 248 702 1496 1349
Fragments arranged by size:
1496 1349 737 702 248 45
BsaWI w'CCGG_w
Cuts at: 1766 2287 2965 3239 3386 3797 1766
Size: 521 678 274 147 411 2546
Fragments arranged by size:
2546 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 847 877 847
Size: 30 4547
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 223 422 223
Size: 199 4378
BseYI C'CCAG_C
Cuts at: 147 500 2002 2760 3337 3833 147
Size: 353 1502 758 577 496 891
Fragments arranged by size:
1502 891 758 577 496 353
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 522 1228 1264 1387 522
Size: 706 36 123 3712
Fragments arranged by size:
3712 706 123 36
BsiEI CG_ry'CG
Cuts at: 104 1567 2119 2165 3373 104
Size: 1463 552 46 1208 1308
Fragments arranged by size:
1463 1308 1208 552 46
BsiHKAI G_wGCw'C
Cuts at: 1591 2369 2559 3351 1591
Size: 778 190 792 2817
Fragments arranged by size:
2817 792 778 190
BsmI GAATG_Cn'
Cuts at: 1176 1176
Size: 4577
BsmBI CGTCTCn'nnnn_
Cuts at: 456 456
Size: 4577
Bsp1286I G_dGCh'C
Cuts at: 618 735 1259 1427 1591 2202 2295 2369 2559
Size: 117 524 168 164 611 93 74 190
Cuts at: 2559 2621 3351 618
Size: 62 730 1844
Fragments arranged by size:
1844 730 611 524 190 168 164 117
93 74 62
BspEI T'CCGG_A
Cuts at: 3797 3797
Size: 4577
BspHI T'CATG_A
Cuts at: 3753 3753
Size: 4577
BspMI ACCTGCnnnn'nnnn_
Cuts at: 230 248 1106 1535 1627 1841 2143 2524 230
Size: 18 858 429 92 214 302 381 2283
Fragments arranged by size:
2283 858 429 381 302 214 92 18
BsrBI CCG'CTC
Cuts at: 50 2869 2923 4407 4572 50
Size: 2819 54 1484 165 55
Fragments arranged by size:
2819 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1238 2489 1238
Size: 1251 3326
BsrFI r'CCGG_y
Cuts at: 2575 2756 2965 2575
Size: 181 209 4187
Fragments arranged by size:
4187 209 181
BsrGI T'GTAC_A
Cuts at: 1127 1127
Size: 4577
BssSI C'ACGA_G
Cuts at: 607 1162 2848 3206 607
Size: 555 1686 358 1978
Fragments arranged by size:
1978 1686 555 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1242 1954 1242
Size: 712 3865
BstBI TT'CG_AA
Cuts at: 2938 2938
Size: 4577
BstXI CCAn_nnnn'nTGG
Cuts at: 561 1470 561
Size: 909 3668
BstYI r'GATC_y
Cuts at: 1 758 1602 2069 2092 2427 2673 3685 3771
Size: 757 844 467 23 335 246 1012 86
Cuts at: 3771 3783 4129 1
Size: 12 346 449
Fragments arranged by size:
1012 844 757 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 347 479 915 1187 107
Size: 240 132 436 272 3497
Fragments arranged by size:
3497 436 272 240 132
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2527 2708 2871 2527
Size: 181 163 4233
Fragments arranged by size:
4233 181 163
BtsI GCAGTG_nn'
Cuts at: 338 547 1820 338
Size: 209 1273 3095
Fragments arranged by size:
3095 1273 209
DraI TTT'AAA
Cuts at: 1576 1576
Size: 4577
DraIII CAC_nnn'GTG
Cuts at: 1164 1164
Size: 4577
DrdI GACnn_nn'nnGTC
Cuts at: 1311 2283 3141 1311
Size: 972 858 2747
Fragments arranged by size:
2747 972 858
EaeI y'GGCC_r
Cuts at: 161 485 509 817 1460 1564 2162 2336 2727
Size: 324 24 308 643 104 598 174 391
Cuts at: 2727 2754 4099 161
Size: 27 1345 639
Fragments arranged by size:
1345 643 639 598 391 324 308 174
104 27 24
EagI C'GGCC_G
Cuts at: 1564 2162 1564
Size: 598 3979
EarI CTCTTCn'nnn_
Cuts at: 943 2600 2810 943
Size: 1657 210 2710
Fragments arranged by size:
2710 1657 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2785 3095 3241 4387 2785
Size: 310 146 1146 2975
Fragments arranged by size:
2975 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1589 1589
Size: 4577
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 283 324 1362 1570 2402 2834 3581 3849 283
Size: 41 1038 208 832 432 747 268 1011
Fragments arranged by size:
1038 1011 832 747 432 268 208 41
EcoNI CCTnn'n_nnAGG
Cuts at: 1408 1408
Size: 4577
EcoO109I rG'GnC_Cy
Cuts at: 875 1455 1716 875
Size: 580 261 3736
Fragments arranged by size:
3736 580 261
EcoRI G'AATT_C
Cuts at: 1581 1581
Size: 4577
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2596 2596
Size: 4577
FspI TGC'GCA
Cuts at: 2358 2358
Size: 4577
HaeII r_GCGC'y
Cuts at: 1340 2011 2259 3281 4260 1340
Size: 671 248 1022 979 1657
Fragments arranged by size:
1657 1022 979 671 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2419 2449 2451 2481 2419
Size: 30 2 30 4515
Fragments arranged by size:
4515 30 30 2
HincII GTy'rAC
Cuts at: 1332 1616 1332
Size: 284 4293
KasI G'GCGC_C
Cuts at: 2007 2255 2007
Size: 248 4329
KpnI G_GTAC'C
Cuts at: 1597 1597
Size: 4577
MluI A'CGCG_T
Cuts at: 4010 4010
Size: 4577
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 890 3248 3432 890
Size: 2358 184 2035
Fragments arranged by size:
2358 2035 184
MscI TGG'CCA
Cuts at: 487 1462 2338 487
Size: 975 876 2726
Fragments arranged by size:
2726 975 876
MslI CAynn'nnrTG
Cuts at: 202 649 1288 1405 1483 2566 2693 202
Size: 447 639 117 78 1083 127 2086
Fragments arranged by size:
2086 1083 639 447 127 117 78
MspA1I CmG'CkG
Cuts at: 349 885 917 1251 1688 2002 2362 3375 3620
Size: 536 32 334 437 314 360 1013 245
Cuts at: 3620 349
Size: 1306
Fragments arranged by size:
1306 1013 536 437 360 334 314 245
32
NaeI GCC'GGC
Cuts at: 2758 2758
Size: 4577
NarI GG'CG_CC
Cuts at: 2008 2256 2008
Size: 248 4329
NcoI C'CATG_G
Cuts at: 107 479 107
Size: 372 4205
NgoMIV G'CCGG_C
Cuts at: 2756 2756
Size: 4577
NspI r_CATG'y
Cuts at: 1386 1630 2130 3037 1386
Size: 244 500 907 2926
Fragments arranged by size:
2926 907 500 244
PasI CC'CwG_GG
Cuts at: 948 1420 948
Size: 472 4105
PciI A'CATG_T
Cuts at: 3033 3033
Size: 4577
PmeI GTTT'AAAC
Cuts at: 1576 1576
Size: 4577
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 847 879 3716 3748 847
Size: 32 2837 32 1676
Fragments arranged by size:
2837 1676 32 32
PpuMI rG'GwC_Cy
Cuts at: 875 1455 875
Size: 580 3997
PshAI GACnn'nnGTC
Cuts at: 1270 1270
Size: 4577
PspOMI G'GGCC_C
Cuts at: 614 614
Size: 4577
PstI C_TGCA'G
Cuts at: 227 931 1532 1624 2309 227
Size: 704 601 92 685 2495
Fragments arranged by size:
2495 704 685 601 92
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4577
PvuII CAG'CTG
Cuts at: 1251 2002 2362 1251
Size: 751 360 3466
Fragments arranged by size:
3466 751 360
RsrII CG'GwC_CG
Cuts at: 2772 2772
Size: 4577
SacI G_AGCT'C
Cuts at: 1591 1591
Size: 4577
SacII CC_GC'GG
Cuts at: 350 918 350
Size: 568 4009
SalI G'TCGA_C
Cuts at: 1614 1614
Size: 4577
SapI GCTCTTCn'nnn_
Cuts at: 943 2600 2810 943
Size: 1657 210 2710
Fragments arranged by size:
2710 1657 210
SbfI CC_TGCA'GG
Cuts at: 227 1624 227
Size: 1397 3180
ScaI AGT'ACT
Cuts at: 4094 4094
Size: 4577
SexAI A'CCwGG_T
Cuts at: 179 179
Size: 4577
SfcI C'TryA_G
Cuts at: 33 223 572 722 927 1437 1528 1620 2305
Size: 190 349 150 205 510 91 92 685
Cuts at: 2305 3298 3489 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 685 510 349 205 191 190
150 92 91
SfiI GGCCn_nnn'nGGCC
Cuts at: 484 592 484
Size: 108 4469
SfoI GGC'GCC
Cuts at: 2009 2257 2009
Size: 248 4329
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4577
SmaI CCC'GGG
Cuts at: 1599 1599
Size: 4577
SmlI C'TyrA_G
Cuts at: 280 1732 2647 3139 3401 280
Size: 1452 915 492 262 1456
Fragments arranged by size:
1456 1452 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4577
SphI G_CATG'C
Cuts at: 1386 1630 2130 1386
Size: 244 500 3833
Fragments arranged by size:
3833 500 244
StyI C'CwwG_G
Cuts at: 107 479 701 1711 2065 107
Size: 372 222 1010 354 2619
Fragments arranged by size:
2619 1010 372 354 222
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 492 2160 2293 2752 2961 492
Size: 1668 133 459 209 2108
Fragments arranged by size:
2108 1668 459 209 133
TatI w'GTAC_w
Cuts at: 1127 4092 1127
Size: 2965 1612
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 410 442 1148 1180 1439 1467 1471 1499 4094
Size: 32 706 32 259 28 4 28 2595
Cuts at: 4094 4126 410
Size: 32 861
Fragments arranged by size:
2595 861 706 259 32 32 32 28
28 4
Tth111I GACn'n_nGTC
Cuts at: 2374 2374
Size: 4577
XbaI T'CTAG_A
Cuts at: 1608 1608
Size: 4577
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 177 2072 177
Size: 1895 2682
XmaI C'CCGG_G
Cuts at: 1597 1597
Size: 4577
XmnI GAAnn'nnTTC
Cuts at: 717 717
Size: 4577
ZraI GAC'GTC
Cuts at: 1272 1272
Size: 4577
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PmeI PpiI PpuMI PshAI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AlfI AscI AvrII BaeI BbsI
BbvCI BcgI BfrBI BmtI Bpu10I BsiWI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PflMI PfoI PmlI PsiI PspXI PsrI SanDI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI