Restriction map for
pF1KE0337
(Circular) (Six-base) MAPSORT of: pF1KE0337.seq Check: 3353 from: 1 to: 4640
pF1KE0337 4640 bp
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AccI GT'mk_AC
Cuts at: 1678 1678
Size: 4640
Acc65I G'GTAC_C
Cuts at: 1656 1656
Size: 4640
AclI AA'CG_TT
Cuts at: 4480 4480
Size: 4640
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1474 2465 2897 3644 3912 1474
Size: 991 432 747 268 2202
Fragments arranged by size:
2202 991 747 432 268
AflIII A'CryG_T
Cuts at: 1992 3096 4073 1992
Size: 1104 977 2559
Fragments arranged by size:
2559 1104 977
AgeI A'CCGG_T
Cuts at: 3028 3028
Size: 4640
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 842 842
Size: 4640
AlwNI CAG_nnn'CTG
Cuts at: 588 639 1348 1945 3512 588
Size: 51 709 597 1567 1716
Fragments arranged by size:
1716 1567 709 597 51
ApaI G_GGCC'C
Cuts at: 577 1152 577
Size: 575 4065
ApaLI G'TGCA_C
Cuts at: 318 3410 318
Size: 3092 1548
ApoI r'AATT_y
Cuts at: 1644 3993 4249 1644
Size: 2349 256 2035
Fragments arranged by size:
2349 2035 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4640
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4640
AvaI C'yCGr_G
Cuts at: 401 452 983 1547 1660 401
Size: 51 531 564 113 3381
Fragments arranged by size:
3381 564 531 113 51
BamHI G'GATC_C
Cuts at: 1665 1665
Size: 4640
BanI G'GyrC_C
Cuts at: 120 233 390 485 1656 2070 2318 2353 120
Size: 113 157 95 1171 414 248 35 2407
Fragments arranged by size:
2407 1171 414 248 157 113 95 35
BanII G_rGCy'C
Cuts at: 577 1152 1219 1321 1388 1654 2684 577
Size: 575 67 102 67 266 1030 2533
Fragments arranged by size:
2533 1030 575 266 102 67 67
BbeI G_GCGC'C
Cuts at: 2074 2322 2074
Size: 248 4392
BbvCI CC'TCA_GC
Cuts at: 1186 1186
Size: 4640
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 974 1008 974
Size: 34 4606
BciVI GTATCCnnnnn_n'
Cuts at: 2538 3299 4640 2538
Size: 761 1341 2538
Fragments arranged by size:
2538 1341 761
BclI T'GATC_A
Cuts at: 2160 2160
Size: 4640
BglI GCCn_nnn'nGGC
Cuts at: 690 690
Size: 4640
BglII A'GATC_T
Cuts at: 1571 2155 4192 1571
Size: 584 2037 2019
Fragments arranged by size:
2037 2019 584
BlpI GC'TnA_GC
Cuts at: 1752 1752
Size: 4640
Bme1580I G_kGCm'C
Cuts at: 123 236 322 395 577 1152 1207 2265 2358
Size: 113 86 73 182 575 55 1058 93
Cuts at: 2358 3414 123
Size: 1056 1349
Fragments arranged by size:
1349 1058 1056 575 182 113 93 86
73 55
BmrI ACTGGGnnnn_n'
Cuts at: 1500 1852 1933 2268 4412 1500
Size: 352 81 335 2144 1728
Fragments arranged by size:
2144 1728 352 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 478 3007 478
Size: 2529 2111
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 439 1347 439
Size: 908 3732
Bpu10I CC'TnA_GC
Cuts at: 1186 1186
Size: 4640
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1483 1816 2695 3187 3485 1483
Size: 333 879 492 298 2638
Fragments arranged by size:
2638 879 492 333 298
BsaI GGTCTCn'nnnn_
Cuts at: 961 1006 4344 961
Size: 45 3338 1257
Fragments arranged by size:
3338 1257 45
BsaAI yAC'GTr
Cuts at: 303 1993 303
Size: 1690 2950
BsaBI GATnn'nnATC
Cuts at: 6 2154 4191 6
Size: 2148 2037 455
Fragments arranged by size:
2148 2037 455
BsaHI Gr'CG_yC
Cuts at: 383 746 2071 2319 3021 4517 383
Size: 363 1325 248 702 1496 506
Fragments arranged by size:
1496 1325 702 506 363 248
BsaWI w'CCGG_w
Cuts at: 997 1829 2350 3028 3302 3449 3860 997
Size: 832 521 678 274 147 411 1777
Fragments arranged by size:
1777 832 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 124 436 124
Size: 312 4328
BseYI C'CCAG_C
Cuts at: 238 394 749 1144 1200 1437 1534 1602 2065
Size: 156 355 395 56 237 97 68 463
Cuts at: 2065 2823 3400 3896 238
Size: 758 577 496 982
Fragments arranged by size:
982 758 577 496 463 395 355 237
156 97 68 56
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1575 1575
Size: 4640
BsiEI CG_ry'CG
Cuts at: 104 351 2182 2228 3436 104
Size: 247 1831 46 1208 1308
Fragments arranged by size:
1831 1308 1208 247 46
BsiHKAI G_wGCw'C
Cuts at: 322 1654 2432 2622 3414 322
Size: 1332 778 190 792 1548
Fragments arranged by size:
1548 1332 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 925 925
Size: 4640
Bsp1286I G_dGCh'C
Cuts at: 123 236 322 395 577 1152 1207 1219 1321
Size: 113 86 73 182 575 55 12 102
Cuts at: 1321 1388 1654 2265 2358 2432 2622 2684 3414
Size: 67 266 611 93 74 190 62 730
Cuts at: 3414 123
Size: 1349
Fragments arranged by size:
1349 730 611 575 266 190 182 113
102 93 86 74 73 67 62 55
12
BspEI T'CCGG_A
Cuts at: 3860 3860
Size: 4640
BspHI T'CATG_A
Cuts at: 1380 3816 1380
Size: 2436 2204
BspMI ACCTGCnnnn'nnnn_
Cuts at: 601 1127 1442 1690 1904 2206 2587 601
Size: 526 315 248 214 302 381 2654
Fragments arranged by size:
2654 526 381 315 302 248 214
BsrBI CCG'CTC
Cuts at: 50 2932 2986 4470 4635 50
Size: 2882 54 1484 165 55
Fragments arranged by size:
2882 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1614 2552 1614
Size: 938 3702
BsrFI r'CCGG_y
Cuts at: 346 350 475 603 2638 2819 3028 346
Size: 4 125 128 2035 181 209 1958
Fragments arranged by size:
2035 1958 209 181 128 125 4
BssSI C'ACGA_G
Cuts at: 1096 2911 3269 1096
Size: 1815 358 2467
Fragments arranged by size:
2467 1815 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1348 2017 1348
Size: 669 3971
BstBI TT'CG_AA
Cuts at: 3001 3001
Size: 4640
BstEII G'GTnAC_C
Cuts at: 426 426
Size: 4640
BstXI CCAn_nnnn'nTGG
Cuts at: 114 1113 1542 114
Size: 999 429 3212
Fragments arranged by size:
3212 999 429
BstYI r'GATC_y
Cuts at: 1 1571 1623 1665 2132 2155 2490 2736 3748
Size: 1570 52 42 467 23 335 246 1012
Cuts at: 3748 3834 3846 4192 1
Size: 86 12 346 449
Fragments arranged by size:
1570 1012 467 449 346 335 246 86
52 42 23 12
Bsu36I CC'TnA_GG
Cuts at: 503 503
Size: 4640
BtgI C'CryG_G
Cuts at: 113 124 431 671 843 1263 113
Size: 11 307 240 172 420 3490
Fragments arranged by size:
3490 420 307 240 172 11
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2590 2771 2934 2590
Size: 181 163 4296
Fragments arranged by size:
4296 181 163
BtsI GCAGTG_nn'
Cuts at: 947 1153 1337 1883 947
Size: 206 184 546 3704
Fragments arranged by size:
3704 546 206 184
ClaI AT'CG_AT
Cuts at: 540 540
Size: 4640
DraI TTT'AAA
Cuts at: 1639 1639
Size: 4640
DrdI GACnn_nn'nnGTC
Cuts at: 2346 3204 2346
Size: 858 3782
EaeI y'GGCC_r
Cuts at: 158 348 434 473 605 659 740 1066 1103
Size: 190 86 39 132 54 81 326 37
Cuts at: 1103 1448 2225 2399 2790 2817 4162 158
Size: 345 777 174 391 27 1345 636
Fragments arranged by size:
1345 777 636 391 345 326 190 174
132 86 81 54 39 37 27
EagI C'GGCC_G
Cuts at: 348 2225 348
Size: 1877 2763
EarI CTCTTCn'nnn_
Cuts at: 923 1237 2663 2873 923
Size: 314 1426 210 2690
Fragments arranged by size:
2690 1426 314 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 161 2848 3158 3304 4450 161
Size: 2687 310 146 1146 351
Fragments arranged by size:
2687 1146 351 310 146
EcoICRI GAG'CTC
Cuts at: 1652 1652
Size: 4640
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 439 1347 1474 2465 2897 3644 3912 439
Size: 908 127 991 432 747 268 1167
Fragments arranged by size:
1167 991 908 747 432 268 127
EcoNI CCTnn'n_nnAGG
Cuts at: 421 1408 421
Size: 987 3653
EcoO109I rG'GnC_Cy
Cuts at: 216 408 499 509 573 574 636 890 912
Size: 192 91 10 64 1 62 254 22
Cuts at: 912 1148 1149 1413 1557 1779 216
Size: 236 1 264 144 222 3077
Fragments arranged by size:
3077 264 254 236 222 192 144 91
64 62 22 10 1 1
EcoRI G'AATT_C
Cuts at: 1644 1644
Size: 4640
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1320 2659 1320
Size: 1339 3301
FspI TGC'GCA
Cuts at: 2421 2421
Size: 4640
HaeII r_GCGC'y
Cuts at: 756 2074 2322 3344 4323 756
Size: 1318 248 1022 979 1073
Fragments arranged by size:
1318 1073 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1563 1595 2482 2512 2514 2544 1563
Size: 32 887 30 2 30 3659
Fragments arranged by size:
3659 887 32 30 30 2
HincII GTy'rAC
Cuts at: 1679 1679
Size: 4640
KasI G'GCGC_C
Cuts at: 2070 2318 2070
Size: 248 4392
KpnI G_GTAC'C
Cuts at: 1660 1660
Size: 4640
MluI A'CGCG_T
Cuts at: 4073 4073
Size: 4640
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3311 3495 3311
Size: 184 4456
MscI TGG'CCA
Cuts at: 661 1068 1105 2401 661
Size: 407 37 1296 2900
Fragments arranged by size:
2900 1296 407 37
MslI CAynn'nnrTG
Cuts at: 112 469 976 2629 2756 112
Size: 357 507 1653 127 1996
Fragments arranged by size:
1996 1653 507 357 127
MspA1I CmG'CkG
Cuts at: 126 267 845 883 1392 1494 1519 1751 2065
Size: 141 578 38 509 102 25 232 314
Cuts at: 2065 2425 3438 3683 126
Size: 360 1013 245 1083
Fragments arranged by size:
1083 1013 578 509 360 314 245 232
141 102 38 25
NaeI GCC'GGC
Cuts at: 352 477 605 2821 352
Size: 125 128 2216 2171
Fragments arranged by size:
2216 2171 128 125
NarI GG'CG_CC
Cuts at: 2071 2319 2071
Size: 248 4392
NcoI C'CATG_G
Cuts at: 113 113
Size: 4640
NgoMIV G'CCGG_C
Cuts at: 350 475 603 2819 350
Size: 125 128 2216 2171
Fragments arranged by size:
2216 2171 128 125
NspI r_CATG'y
Cuts at: 375 1693 2193 3100 375
Size: 1318 500 907 1915
Fragments arranged by size:
1915 1318 907 500
PasI CC'CwG_GG
Cuts at: 1314 1314
Size: 4640
PciI A'CATG_T
Cuts at: 3096 3096
Size: 4640
PflMI CCAn_nnn'nTGG
Cuts at: 639 1101 639
Size: 462 4178
PmeI GTTT'AAAC
Cuts at: 1639 1639
Size: 4640
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3779 3811 3779
Size: 32 4608
PpuMI rG'GwC_Cy
Cuts at: 216 890 912 1413 1557 216
Size: 674 22 501 144 3299
Fragments arranged by size:
3299 674 501 144 22
PshAI GACnn'nnGTC
Cuts at: 937 937
Size: 4640
PspOMI G'GGCC_C
Cuts at: 573 1148 573
Size: 575 4065
PstI C_TGCA'G
Cuts at: 954 1344 1571 1687 2372 954
Size: 390 227 116 685 3222
Fragments arranged by size:
3222 685 390 227 116
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4640
PvuII CAG'CTG
Cuts at: 267 883 1494 1519 2065 2425 267
Size: 616 611 25 546 360 2482
Fragments arranged by size:
2482 616 611 546 360 25
RsrII CG'GwC_CG
Cuts at: 2835 2835
Size: 4640
SacI G_AGCT'C
Cuts at: 1654 1654
Size: 4640
SacII CC_GC'GG
Cuts at: 127 846 127
Size: 719 3921
SalI G'TCGA_C
Cuts at: 1677 1677
Size: 4640
SanDI GG'GwC_CC
Cuts at: 890 890
Size: 4640
SapI GCTCTTCn'nnn_
Cuts at: 2663 2873 2663
Size: 210 4430
SbfI CC_TGCA'GG
Cuts at: 1687 1687
Size: 4640
ScaI AGT'ACT
Cuts at: 4157 4157
Size: 4640
SfcI C'TryA_G
Cuts at: 33 950 1340 1567 1683 2368 3361 3552 33
Size: 917 390 227 116 685 993 191 1121
Fragments arranged by size:
1121 993 917 685 390 227 191 116
SfoI GGC'GCC
Cuts at: 2072 2320 2072
Size: 248 4392
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4640
SmaI CCC'GGG
Cuts at: 403 1549 1662 403
Size: 1146 113 3381
Fragments arranged by size:
3381 1146 113
SmlI C'TyrA_G
Cuts at: 1498 1795 2710 3202 3464 1498
Size: 297 915 492 262 2674
Fragments arranged by size:
2674 915 492 297 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4640
SphI G_CATG'C
Cuts at: 1693 2193 1693
Size: 500 4140
StyI C'CwwG_G
Cuts at: 113 489 1055 1169 1409 1774 2128 113
Size: 376 566 114 240 365 354 2625
Fragments arranged by size:
2625 566 376 365 354 240 114
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 811 1327 2223 2356 2815 3024 811
Size: 516 896 133 459 209 2427
Fragments arranged by size:
2427 896 516 459 209 133
TatI w'GTAC_w
Cuts at: 776 1292 4155 776
Size: 516 2863 1261
Fragments arranged by size:
2863 1261 516
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4157 4189 4157
Size: 32 4608
Tth111I GACn'n_nGTC
Cuts at: 1258 2437 1258
Size: 1179 3461
XbaI T'CTAG_A
Cuts at: 1671 1671
Size: 4640
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 255 654 669 912 1167 2135 255
Size: 399 15 243 255 968 2760
Fragments arranged by size:
2760 968 399 255 243 15
XmaI C'CCGG_G
Cuts at: 401 1547 1660 401
Size: 1146 113 3381
Fragments arranged by size:
3381 1146 113
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BbsI BfrBI BmgBI BmtI BsaXI BsiWI
BsmI BsrGI BssHII BstZ17I CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PfoI PmlI PsiI PspXI PsrI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI