Restriction map for
pF1KE0340
(Circular) (Six-base) MAPSORT of: pF1KE0340.seq Check: 8437 from: 1 to: 4634
pF1KE0340 4634 bp
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AccI GT'mk_AC
Cuts at: 644 1672 644
Size: 1028 3606
Acc65I G'GTAC_C
Cuts at: 1650 1650
Size: 4634
AclI AA'CG_TT
Cuts at: 4474 4474
Size: 4634
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 499 2459 2891 3638 3906 190
Size: 309 1960 432 747 268 918
Fragments arranged by size:
1960 918 747 432 309 268
AflIII A'CryG_T
Cuts at: 1138 1986 3090 4067 1138
Size: 848 1104 977 1705
Fragments arranged by size:
1705 1104 977 848
AgeI A'CCGG_T
Cuts at: 3022 3022
Size: 4634
AleI CACnn'nnGTG
Cuts at: 1143 1143
Size: 4634
AlwNI CAG_nnn'CTG
Cuts at: 771 822 1939 3506 771
Size: 51 1117 1567 1899
Fragments arranged by size:
1899 1567 1117 51
ApaLI G'TGCA_C
Cuts at: 3404 3404
Size: 4634
ApoI r'AATT_y
Cuts at: 667 967 1638 3987 4243 667
Size: 300 671 2349 256 1058
Fragments arranged by size:
2349 1058 671 300 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4634
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4634
AvaI C'yCGr_G
Cuts at: 1384 1654 1384
Size: 270 4364
BamHI G'GATC_C
Cuts at: 1659 1659
Size: 4634
BanI G'GyrC_C
Cuts at: 1650 2064 2312 2347 1650
Size: 414 248 35 3937
Fragments arranged by size:
3937 414 248 35
BanII G_rGCy'C
Cuts at: 984 1463 1648 2678 984
Size: 479 185 1030 2940
Fragments arranged by size:
2940 1030 479 185
BbeI G_GCGC'C
Cuts at: 2068 2316 2068
Size: 248 4386
BbsI GAAGACnn'nnnn_
Cuts at: 1106 1106
Size: 4634
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4600
BciVI GTATCCnnnnn_n'
Cuts at: 2532 3293 4634 2532
Size: 761 1341 2532
Fragments arranged by size:
2532 1341 761
BclI T'GATC_A
Cuts at: 842 2154 842
Size: 1312 3322
BglI GCCn_nnn'nGGC
Cuts at: 527 1342 527
Size: 815 3819
BglII A'GATC_T
Cuts at: 2149 4186 2149
Size: 2037 2597
BlpI GC'TnA_GC
Cuts at: 1746 1746
Size: 4634
Bme1580I G_kGCm'C
Cuts at: 2259 2352 3408 2259
Size: 93 1056 3485
Fragments arranged by size:
3485 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 575 1846 1927 2262 4406 575
Size: 1271 81 335 2144 803
Fragments arranged by size:
2144 1271 803 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3001 3001
Size: 4634
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 284 659 1391 284
Size: 375 732 3527
Fragments arranged by size:
3527 732 375
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 353 1810 2689 3181 3479 353
Size: 1457 879 492 298 1508
Fragments arranged by size:
1508 1457 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4338 4338
Size: 4634
BsaAI yAC'GTr
Cuts at: 1141 1145 1987 1141
Size: 4 842 3788
Fragments arranged by size:
3788 842 4
BsaBI GATnn'nnATC
Cuts at: 6 1447 2148 4185 6
Size: 1441 701 2037 455
Fragments arranged by size:
2037 1441 701 455
BsaHI Gr'CG_yC
Cuts at: 2065 2313 3015 4511 2065
Size: 248 702 1496 2188
Fragments arranged by size:
2188 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1823 2344 3022 3296 3443 3854 1823
Size: 521 678 274 147 411 2603
Fragments arranged by size:
2603 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 818 848 818
Size: 30 4604
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 268 268
Size: 4634
BseYI C'CCAG_C
Cuts at: 253 880 2059 2817 3394 3890 253
Size: 627 1179 758 577 496 997
Fragments arranged by size:
1179 997 758 627 577 496
BsiEI CG_ry'CG
Cuts at: 104 2176 2222 3430 104
Size: 2072 46 1208 1308
Fragments arranged by size:
2072 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 407 1648 2426 2616 3408 407
Size: 1241 778 190 792 1633
Fragments arranged by size:
1633 1241 792 778 190
BsiWI C'GTAC_G
Cuts at: 1141 1141
Size: 4634
BsmI GAATG_Cn'
Cuts at: 503 875 979 503
Size: 372 104 4158
Fragments arranged by size:
4158 372 104
BsmBI CGTCTCn'nnnn_
Cuts at: 694 694
Size: 4634
Bsp1286I G_dGCh'C
Cuts at: 407 984 1463 1648 2259 2352 2426 2616 2678
Size: 577 479 185 611 93 74 190 62
Cuts at: 2678 3408 407
Size: 730 1633
Fragments arranged by size:
1633 730 611 577 479 190 185 93
74 62
BspEI T'CCGG_A
Cuts at: 3854 3854
Size: 4634
BspHI T'CATG_A
Cuts at: 1310 3810 1310
Size: 2500 2134
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1151 1684 1898 2200 2581 1151
Size: 533 214 302 381 3204
Fragments arranged by size:
3204 533 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2926 2980 4464 4629 50
Size: 2876 54 1484 165 55
Fragments arranged by size:
2876 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2546 2546
Size: 4634
BsrFI r'CCGG_y
Cuts at: 2632 2813 3022 2632
Size: 181 209 4244
Fragments arranged by size:
4244 209 181
BssSI C'ACGA_G
Cuts at: 983 2905 3263 983
Size: 1922 358 2354
Fragments arranged by size:
2354 1922 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2011 2011
Size: 4634
BstBI TT'CG_AA
Cuts at: 2995 2995
Size: 4634
BstYI r'GATC_y
Cuts at: 1 576 1205 1659 2126 2149 2484 2730 3742
Size: 575 629 454 467 23 335 246 1012
Cuts at: 3742 3828 3840 4186 1
Size: 86 12 346 449
Fragments arranged by size:
1012 629 575 467 454 449 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1175 1175
Size: 4634
BtgI C'CryG_G
Cuts at: 159 159
Size: 4634
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2584 2765 2928 2584
Size: 181 163 4290
Fragments arranged by size:
4290 181 163
BtsI GCAGTG_nn'
Cuts at: 1877 1877
Size: 4634
DraI TTT'AAA
Cuts at: 1633 1633
Size: 4634
DraIII CAC_nnn'GTG
Cuts at: 1053 1053
Size: 4634
DrdI GACnn_nn'nnGTC
Cuts at: 2340 3198 2340
Size: 858 3776
EaeI y'GGCC_r
Cuts at: 1147 1374 2219 2393 2784 2811 4156 1147
Size: 227 845 174 391 27 1345 1625
Fragments arranged by size:
1625 1345 845 391 227 174 27
EagI C'GGCC_G
Cuts at: 2219 2219
Size: 4634
EarI CTCTTCn'nnn_
Cuts at: 2657 2867 2657
Size: 210 4424
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2842 3152 3298 4444 2842
Size: 310 146 1146 3032
Fragments arranged by size:
3032 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1646 1646
Size: 4634
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 284 499 659 1391 2459 2891 3638 3906
Size: 94 215 160 732 1068 432 747 268
Cuts at: 3906 190
Size: 918
Fragments arranged by size:
1068 918 747 732 432 268 215 160
94
EcoNI CCTnn'n_nnAGG
Cuts at: 775 775
Size: 4634
EcoO109I rG'GnC_Cy
Cuts at: 731 1773 731
Size: 1042 3592
EcoRI G'AATT_C
Cuts at: 1638 1638
Size: 4634
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 574 967 2653 574
Size: 393 1686 2555
Fragments arranged by size:
2555 1686 393
FspI TGC'GCA
Cuts at: 2415 2415
Size: 4634
HaeII r_GCGC'y
Cuts at: 834 2068 2316 3338 4317 834
Size: 1234 248 1022 979 1151
Fragments arranged by size:
1234 1151 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 834 866 2476 2506 2508 2538 834
Size: 32 1610 30 2 30 2930
Fragments arranged by size:
2930 1610 32 30 30 2
HincII GTy'rAC
Cuts at: 1673 1673
Size: 4634
HindIII A'AGCT_T
Cuts at: 1245 1291 1245
Size: 46 4588
KasI G'GCGC_C
Cuts at: 2064 2312 2064
Size: 248 4386
KpnI G_GTAC'C
Cuts at: 1654 1654
Size: 4634
MfeI C'AATT_G
Cuts at: 1454 1454
Size: 4634
MluI A'CGCG_T
Cuts at: 4067 4067
Size: 4634
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 434 3305 3489 434
Size: 2871 184 1579
Fragments arranged by size:
2871 1579 184
MscI TGG'CCA
Cuts at: 1149 1376 2395 1149
Size: 227 1019 3388
Fragments arranged by size:
3388 1019 227
MslI CAynn'nnrTG
Cuts at: 1143 2623 2750 1143
Size: 1480 127 3027
Fragments arranged by size:
3027 1480 127
MspA1I CmG'CkG
Cuts at: 145 161 822 1745 2059 2419 3432 3677 145
Size: 16 661 923 314 360 1013 245 1102
Fragments arranged by size:
1102 1013 923 661 360 314 245 16
NaeI GCC'GGC
Cuts at: 2815 2815
Size: 4634
NarI GG'CG_CC
Cuts at: 2065 2313 2065
Size: 248 4386
NgoMIV G'CCGG_C
Cuts at: 2813 2813
Size: 4634
NspI r_CATG'y
Cuts at: 742 1416 1687 2187 3094 742
Size: 674 271 500 907 2282
Fragments arranged by size:
2282 907 674 500 271
PasI CC'CwG_GG
Cuts at: 1237 1237
Size: 4634
PciI A'CATG_T
Cuts at: 3090 3090
Size: 4634
PflMI CCAn_nnn'nTGG
Cuts at: 113 396 1156 113
Size: 283 760 3591
Fragments arranged by size:
3591 760 283
PfoI T'CCnGG_A
Cuts at: 300 300
Size: 4634
PmeI GTTT'AAAC
Cuts at: 1633 1633
Size: 4634
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3773 3805 3773
Size: 32 4602
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 168 200 168
Size: 32 4602
PstI C_TGCA'G
Cuts at: 858 1436 1578 1681 2366 858
Size: 578 142 103 685 3126
Fragments arranged by size:
3126 685 578 142 103
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4634
PvuII CAG'CTG
Cuts at: 822 2059 2419 822
Size: 1237 360 3037
Fragments arranged by size:
3037 1237 360
RsrII CG'GwC_CG
Cuts at: 2829 2829
Size: 4634
SacI G_AGCT'C
Cuts at: 1648 1648
Size: 4634
SacII CC_GC'GG
Cuts at: 162 162
Size: 4634
SalI G'TCGA_C
Cuts at: 1671 1671
Size: 4634
SapI GCTCTTCn'nnn_
Cuts at: 2657 2867 2657
Size: 210 4424
SbfI CC_TGCA'GG
Cuts at: 1681 1681
Size: 4634
ScaI AGT'ACT
Cuts at: 4151 4151
Size: 4634
SfcI C'TryA_G
Cuts at: 33 146 854 1260 1432 1574 1677 2362 3355
Size: 113 708 406 172 142 103 685 993
Cuts at: 3355 3546 33
Size: 191 1121
Fragments arranged by size:
1121 993 708 685 406 191 172 142
113 103
SfoI GGC'GCC
Cuts at: 2066 2314 2066
Size: 248 4386
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4634
SmaI CCC'GGG
Cuts at: 1656 1656
Size: 4634
SmlI C'TyrA_G
Cuts at: 368 1789 2704 3196 3458 368
Size: 1421 915 492 262 1544
Fragments arranged by size:
1544 1421 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4634
SphI G_CATG'C
Cuts at: 1687 2187 1687
Size: 500 4134
StyI C'CwwG_G
Cuts at: 112 1768 2122 112
Size: 1656 354 2624
Fragments arranged by size:
2624 1656 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 284 1022 2217 2350 2809 3018 284
Size: 738 1195 133 459 209 1900
Fragments arranged by size:
1900 1195 738 459 209 133
TatI w'GTAC_w
Cuts at: 4149 4149
Size: 4634
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1550 1582 4151 4183 1550
Size: 32 2569 32 2001
Fragments arranged by size:
2569 2001 32 32
Tth111I GACn'n_nGTC
Cuts at: 2431 2431
Size: 4634
XbaI T'CTAG_A
Cuts at: 1200 1665 1200
Size: 465 4169
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2129 2129
Size: 4634
XmaI C'CCGG_G
Cuts at: 1654 1654
Size: 4634
XmnI GAAnn'nnTTC
Cuts at: 668 947 1196 668
Size: 279 249 4106
Fragments arranged by size:
4106 279 249
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsiWI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PpiI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BfrBI BmgBI BmtI Bpu10I
BsgI BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI
EcoRV FseI FspAI HpaI NcoI NdeI NheI NotI
NruI NsiI PacI PmlI PpuMI PshAI PsiI PspOMI
PspXI SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI