Restriction map for
pF1KE0341
(Circular) (Six-base) MAPSORT of: pF1KE0341.seq Check: 1195 from: 1 to: 5165
pF1KE0341 5165 bp
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AatII G_ACGT'C
Cuts at: 1637 1637
Size: 5165
AccI GT'mk_AC
Cuts at: 2203 2203
Size: 5165
Acc65I G'GTAC_C
Cuts at: 1961 2181 1961
Size: 220 4945
AclI AA'CG_TT
Cuts at: 5005 5005
Size: 5165
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 423 994 2990 3422 4169 4437 423
Size: 571 1996 432 747 268 1151
Fragments arranged by size:
1996 1151 747 571 432 268
AfeI AGC'GCT
Cuts at: 179 179
Size: 5165
AflIII A'CryG_T
Cuts at: 2517 3621 4598 2517
Size: 1104 977 3084
Fragments arranged by size:
3084 1104 977
AgeI A'CCGG_T
Cuts at: 1649 3553 1649
Size: 1904 3261
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1923 2044 1923
Size: 121 5044
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1729 1761 1729
Size: 32 5133
AlwNI CAG_nnn'CTG
Cuts at: 1536 2470 4037 1536
Size: 934 1567 2664
Fragments arranged by size:
2664 1567 934
ApaI G_GGCC'C
Cuts at: 227 294 227
Size: 67 5098
ApaLI G'TGCA_C
Cuts at: 3935 3935
Size: 5165
ApoI r'AATT_y
Cuts at: 1009 1491 1611 2169 4518 4774 1009
Size: 482 120 558 2349 256 1400
Fragments arranged by size:
2349 1400 558 482 256 120
AseI AT'TA_AT
Cuts at: 20 20
Size: 5165
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5165
AvaI C'yCGr_G
Cuts at: 144 842 943 1173 2185 144
Size: 698 101 230 1012 3124
Fragments arranged by size:
3124 1012 698 230 101
BamHI G'GATC_C
Cuts at: 2190 2190
Size: 5165
BanI G'GyrC_C
Cuts at: 186 329 1961 2054 2181 2595 2843 2878 186
Size: 143 1632 93 127 414 248 35 2473
Fragments arranged by size:
2473 1632 414 248 143 127 93 35
BanII G_rGCy'C
Cuts at: 227 294 521 1388 1445 1583 2159 2179 3209
Size: 67 227 867 57 138 576 20 1030
Cuts at: 3209 227
Size: 2183
Fragments arranged by size:
2183 1030 867 576 227 138 67 57
20
BbeI G_GCGC'C
Cuts at: 2058 2599 2847 2058
Size: 541 248 4376
Fragments arranged by size:
4376 541 248
BbsI GAAGACnn'nnnn_
Cuts at: 1629 1808 1629
Size: 179 4986
BciVI GTATCCnnnnn_n'
Cuts at: 903 3063 3824 5165 903
Size: 2160 761 1341 903
Fragments arranged by size:
2160 1341 903 761
BclI T'GATC_A
Cuts at: 2685 2685
Size: 5165
BfrBI ATG'CAT
Cuts at: 1183 1183
Size: 5165
BglII A'GATC_T
Cuts at: 2680 4717 2680
Size: 2037 3128
BlpI GC'TnA_GC
Cuts at: 415 832 2277 415
Size: 417 1445 3303
Fragments arranged by size:
3303 1445 417
Bme1580I G_kGCm'C
Cuts at: 191 227 294 1100 1664 1838 2790 2883 3939
Size: 36 67 806 564 174 952 93 1056
Cuts at: 3939 191
Size: 1417
Fragments arranged by size:
1417 1056 952 806 564 174 93 67
36
BmgBI CAC'GTC
Cuts at: 552 552
Size: 5165
BmrI ACTGGGnnnn_n'
Cuts at: 2377 2458 2793 4937 2377
Size: 81 335 2144 2605
Fragments arranged by size:
2605 2144 335 81
BmtI G_CTAG'C
Cuts at: 1217 1217
Size: 5165
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3532 3532
Size: 5165
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1366 1534 1567 1882 2080 2099 1366
Size: 168 33 315 198 19 4432
Fragments arranged by size:
4432 315 198 168 33 19
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 907 2341 3220 3712 4010 907
Size: 1434 879 492 298 2062
Fragments arranged by size:
2062 1434 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 872 4869 872
Size: 3997 1168
BsaAI yAC'GTr
Cuts at: 2518 2518
Size: 5165
BsaBI GATnn'nnATC
Cuts at: 6 2679 4716 6
Size: 2673 2037 455
Fragments arranged by size:
2673 2037 455
BsaHI Gr'CG_yC
Cuts at: 470 1634 2055 2596 2844 3546 5042 470
Size: 1164 421 541 248 702 1496 593
Fragments arranged by size:
1496 1164 702 593 541 421 248
BsaWI w'CCGG_w
Cuts at: 1280 1649 2354 2875 3553 3827 3974 4385 1280
Size: 369 705 521 678 274 147 411 2060
Fragments arranged by size:
2060 705 678 521 411 369 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1729 1759 1729
Size: 30 5135
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1531 2167 1531
Size: 636 4529
BseYI C'CCAG_C
Cuts at: 174 213 220 513 1291 1541 2590 3348 3925
Size: 39 7 293 778 250 1049 758 577
Cuts at: 3925 4421 174
Size: 496 918
Fragments arranged by size:
1049 918 778 758 577 496 293 250
39 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 651 1739 154
Size: 497 1088 3580
Fragments arranged by size:
3580 1088 497
BsiEI CG_ry'CG
Cuts at: 104 1832 2707 2753 3961 104
Size: 1728 875 46 1208 1308
Fragments arranged by size:
1728 1308 1208 875 46
BsiHKAI G_wGCw'C
Cuts at: 1352 1388 2159 2179 2957 3147 3939 1352
Size: 36 771 20 778 190 792 2578
Fragments arranged by size:
2578 792 778 771 190 36 20
BsmI GAATG_Cn'
Cuts at: 1183 1183
Size: 5165
Bsp1286I G_dGCh'C
Cuts at: 191 227 294 521 1100 1352 1388 1445 1583
Size: 36 67 227 579 252 36 57 138
Cuts at: 1583 1664 1838 2159 2179 2790 2883 2957 3147
Size: 81 174 321 20 611 93 74 190
Cuts at: 3147 3209 3939 191
Size: 62 730 1417
Fragments arranged by size:
1417 730 611 579 321 252 227 190
174 138 93 81 74 67 62 57
36 36 20
BspEI T'CCGG_A
Cuts at: 4385 4385
Size: 5165
BspHI T'CATG_A
Cuts at: 4341 4341
Size: 5165
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2215 2429 2731 3112 2215
Size: 214 302 381 4268
Fragments arranged by size:
4268 381 302 214
BsrBI CCG'CTC
Cuts at: 50 288 839 3457 3511 4995 5160 50
Size: 238 551 2618 54 1484 165 55
Fragments arranged by size:
2618 1484 551 238 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3077 3077
Size: 5165
BsrFI r'CCGG_y
Cuts at: 1337 1649 3163 3344 3553 1337
Size: 312 1514 181 209 2949
Fragments arranged by size:
2949 1514 312 209 181
BssHII G'CGCG_C
Cuts at: 258 258
Size: 5165
BssSI C'ACGA_G
Cuts at: 1981 3436 3794 1981
Size: 1455 358 3352
Fragments arranged by size:
3352 1455 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2542 2542
Size: 5165
BstBI TT'CG_AA
Cuts at: 3526 3526
Size: 5165
BstXI CCAn_nnnn'nTGG
Cuts at: 874 2080 874
Size: 1206 3959
BstYI r'GATC_y
Cuts at: 1 960 2190 2657 2680 3015 3261 4273 4359
Size: 959 1230 467 23 335 246 1012 86
Cuts at: 4359 4371 4717 1
Size: 12 346 449
Fragments arranged by size:
1230 1012 959 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 731 1966 731
Size: 1235 3930
BtgI C'CryG_G
Cuts at: 107 227 295 325 722 1305 1496 1778 107
Size: 120 68 30 397 583 191 282 3494
Fragments arranged by size:
3494 583 397 282 191 120 68 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 147 528 3115 3296 3459 147
Size: 381 2587 181 163 1853
Fragments arranged by size:
2587 1853 381 181 163
BtsI GCAGTG_nn'
Cuts at: 284 2408 284
Size: 2124 3041
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1485 1520 1485
Size: 35 5130
DraI TTT'AAA
Cuts at: 2164 2164
Size: 5165
DraIII CAC_nnn'GTG
Cuts at: 1272 1797 2013 1272
Size: 525 216 4424
Fragments arranged by size:
4424 525 216
DrdI GACnn_nn'nnGTC
Cuts at: 1633 2871 3729 1633
Size: 1238 858 3069
Fragments arranged by size:
3069 1238 858
EaeI y'GGCC_r
Cuts at: 158 585 965 1466 1781 2750 2924 3315 3342
Size: 427 380 501 315 969 174 391 27
Cuts at: 3342 4687 158
Size: 1345 636
Fragments arranged by size:
1345 969 636 501 427 391 380 315
174 27
EagI C'GGCC_G
Cuts at: 2750 2750
Size: 5165
EarI CTCTTCn'nnn_
Cuts at: 442 1588 3188 3398 442
Size: 1146 1600 210 2209
Fragments arranged by size:
2209 1600 1146 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 382 1738 3373 3683 3829 4975 382
Size: 1356 1635 310 146 1146 572
Fragments arranged by size:
1635 1356 1146 572 310 146
EcoICRI GAG'CTC
Cuts at: 1386 2157 2177 1386
Size: 771 20 4374
Fragments arranged by size:
4374 771 20
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 423 994 1366 1534 1567 1882 2080 2099 2990
Size: 571 372 168 33 315 198 19 891
Cuts at: 2990 3422 4169 4437 423
Size: 432 747 268 1151
Fragments arranged by size:
1151 891 747 571 432 372 315 268
198 168 33 19
EcoNI CCTnn'n_nnAGG
Cuts at: 1063 1483 1063
Size: 420 4745
EcoO109I rG'GnC_Cy
Cuts at: 139 291 2119 2304 139
Size: 152 1828 185 3000
Fragments arranged by size:
3000 1828 185 152
EcoRI G'AATT_C
Cuts at: 1491 2169 1491
Size: 678 4487
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1653 3184 1653
Size: 1531 3634
FspI TGC'GCA
Cuts at: 2946 2946
Size: 5165
HaeII r_GCGC'y
Cuts at: 181 221 1857 2058 2599 2847 3869 4848 181
Size: 40 1636 201 541 248 1022 979 498
Fragments arranged by size:
1636 1022 979 541 498 248 201 40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 391 423 703 735 750 782 1636 1668 3007
Size: 32 280 32 15 32 854 32 1339
Cuts at: 3007 3037 3039 3069 391
Size: 30 2 30 2487
Fragments arranged by size:
2487 1339 854 280 32 32 32 32
30 30 15 2
HincII GTy'rAC
Cuts at: 678 2204 678
Size: 1526 3639
HindIII A'AGCT_T
Cuts at: 1890 1890
Size: 5165
KasI G'GCGC_C
Cuts at: 2054 2595 2843 2054
Size: 541 248 4376
Fragments arranged by size:
4376 541 248
KpnI G_GTAC'C
Cuts at: 1965 2185 1965
Size: 220 4945
MluI A'CGCG_T
Cuts at: 4598 4598
Size: 5165
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 855 3836 4020 855
Size: 2981 184 2000
Fragments arranged by size:
2981 2000 184
MscI TGG'CCA
Cuts at: 160 587 967 2926 160
Size: 427 380 1959 2399
Fragments arranged by size:
2399 1959 427 380
MslI CAynn'nnrTG
Cuts at: 1093 1531 1594 3154 3281 1093
Size: 438 63 1560 127 2977
Fragments arranged by size:
2977 1560 438 127 63
MspA1I CmG'CkG
Cuts at: 121 229 297 327 1118 1203 1401 1545 2276
Size: 108 68 30 791 85 198 144 731
Cuts at: 2276 2590 2950 3963 4208 121
Size: 314 360 1013 245 1078
Fragments arranged by size:
1078 1013 791 731 360 314 245 198
144 108 85 68 30
NaeI GCC'GGC
Cuts at: 3346 3346
Size: 5165
NarI GG'CG_CC
Cuts at: 2055 2596 2844 2055
Size: 541 248 4376
Fragments arranged by size:
4376 541 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 5165
NdeI CA'TA_TG
Cuts at: 827 827
Size: 5165
NgoMIV G'CCGG_C
Cuts at: 3344 3344
Size: 5165
NheI G'CTAG_C
Cuts at: 1213 1213
Size: 5165
NsiI A_TGCA'T
Cuts at: 1185 1185
Size: 5165
NspI r_CATG'y
Cuts at: 2218 2718 3625 2218
Size: 500 907 3758
Fragments arranged by size:
3758 907 500
PciI A'CATG_T
Cuts at: 3621 3621
Size: 5165
PflMI CCAn_nnn'nTGG
Cuts at: 1272 1272
Size: 5165
PfoI T'CCnGG_A
Cuts at: 1016 1343 1859 1016
Size: 327 516 4322
Fragments arranged by size:
4322 516 327
PmeI GTTT'AAAC
Cuts at: 2164 2164
Size: 5165
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1729 1761 4304 4336 1729
Size: 32 2543 32 2558
Fragments arranged by size:
2558 2543 32 32
PpuMI rG'GwC_Cy
Cuts at: 139 139
Size: 5165
PspOMI G'GGCC_C
Cuts at: 223 290 223
Size: 67 5098
PspXI vC'TCGA_Gb
Cuts at: 943 943
Size: 5165
PstI C_TGCA'G
Cuts at: 239 257 2212 2897 239
Size: 18 1955 685 2507
Fragments arranged by size:
2507 1955 685 18
PvuI CG_AT'CG
Cuts at: 104 1832 104
Size: 1728 3437
PvuII CAG'CTG
Cuts at: 1401 1545 2590 2950 1401
Size: 144 1045 360 3616
Fragments arranged by size:
3616 1045 360 144
RsrII CG'GwC_CG
Cuts at: 3360 3360
Size: 5165
SacI G_AGCT'C
Cuts at: 1388 2159 2179 1388
Size: 771 20 4374
Fragments arranged by size:
4374 771 20
SacII CC_GC'GG
Cuts at: 230 298 328 230
Size: 68 30 5067
Fragments arranged by size:
5067 68 30
SalI G'TCGA_C
Cuts at: 2202 2202
Size: 5165
SanDI GG'GwC_CC
Cuts at: 139 139
Size: 5165
SapI GCTCTTCn'nnn_
Cuts at: 442 1588 3188 3398 442
Size: 1146 1600 210 2209
Fragments arranged by size:
2209 1600 1146 210
SbfI CC_TGCA'GG
Cuts at: 2212 2212
Size: 5165
ScaI AGT'ACT
Cuts at: 4682 4682
Size: 5165
SexAI A'CCwGG_T
Cuts at: 671 671
Size: 5165
SfcI C'TryA_G
Cuts at: 33 235 253 1713 2208 2893 3886 4077 33
Size: 202 18 1460 495 685 993 191 1121
Fragments arranged by size:
1460 1121 993 685 495 202 191 18
SfoI GGC'GCC
Cuts at: 2056 2597 2845 2056
Size: 541 248 4376
Fragments arranged by size:
4376 541 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5165
SmaI CCC'GGG
Cuts at: 146 844 2187 146
Size: 698 1343 3124
Fragments arranged by size:
3124 1343 698
SmlI C'TyrA_G
Cuts at: 922 943 2320 3235 3727 3989 922
Size: 21 1377 915 492 262 2098
Fragments arranged by size:
2098 1377 915 492 262 21
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5165
SphI G_CATG'C
Cuts at: 2218 2718 2218
Size: 500 4665
StuI AGG'CCT
Cuts at: 577 577
Size: 5165
StyI C'CwwG_G
Cuts at: 107 2299 2653 107
Size: 2192 354 2619
Fragments arranged by size:
2619 2192 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 188 484 584 796 880 2748 2881 3340 3549
Size: 296 100 212 84 1868 133 459 209
Cuts at: 3549 188
Size: 1804
Fragments arranged by size:
1868 1804 459 296 212 209 133 100
84
TatI w'GTAC_w
Cuts at: 530 4680 530
Size: 4150 1015
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4682 4714 4682
Size: 32 5133
Tth111I GACn'n_nGTC
Cuts at: 2962 2962
Size: 5165
XbaI T'CTAG_A
Cuts at: 2196 2196
Size: 5165
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1269 1533 2660 1269
Size: 264 1127 3774
Fragments arranged by size:
3774 1127 264
XhoI C'TCGA_G
Cuts at: 943 943
Size: 5165
XmaI C'CCGG_G
Cuts at: 144 842 2185 144
Size: 698 1343 3124
Fragments arranged by size:
3124 1343 698
XmnI GAAnn'nnTTC
Cuts at: 1012 1012
Size: 5165
ZraI GAC'GTC
Cuts at: 1635 1635
Size: 5165
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BciVI BclI
BfrBI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NsiI
NspI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AflII AhdI AleI AscI AvrII BaeI BbvCI
BcgI BglI Bpu10I BsiWI BsmBI BsrGI BstEII BstZ17I
ClaI EcoRV FseI FspAI HpaI MfeI NotI NruI
PacI PasI PmlI PshAI PsiI PsrI SfiI SgrAI
SnaBI SrfI SspI SwaI