Restriction map for
pF1KE0342
(Circular) (Six-base) MAPSORT of: pF1KE0342.seq Check: 9357 from: 1 to: 4988
pF1KE0342 4988 bp
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 675 957 675
Size: 282 4706
AatII G_ACGT'C
Cuts at: 374 374
Size: 4988
AccI GT'mk_AC
Cuts at: 2026 2026
Size: 4988
Acc65I G'GTAC_C
Cuts at: 2004 2004
Size: 4988
AclI AA'CG_TT
Cuts at: 4828 4828
Size: 4988
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 348 1162 2813 3245 3992 4260 348
Size: 814 1651 432 747 268 1076
Fragments arranged by size:
1651 1076 814 747 432 268
AfeI AGC'GCT
Cuts at: 462 462
Size: 4988
AflIII A'CryG_T
Cuts at: 913 1421 2340 3444 4421 913
Size: 508 919 1104 977 1480
Fragments arranged by size:
1480 1104 977 919 508
AgeI A'CCGG_T
Cuts at: 1277 3376 1277
Size: 2099 2889
AleI CACnn'nnGTG
Cuts at: 1594 1594
Size: 4988
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 716 716
Size: 4988
AlwNI CAG_nnn'CTG
Cuts at: 690 1116 1504 2293 3860 690
Size: 426 388 789 1567 1818
Fragments arranged by size:
1818 1567 789 426 388
ApaI G_GGCC'C
Cuts at: 136 1392 1907 136
Size: 1256 515 3217
Fragments arranged by size:
3217 1256 515
ApaLI G'TGCA_C
Cuts at: 943 3758 943
Size: 2815 2173
ApoI r'AATT_y
Cuts at: 1992 4341 4597 1992
Size: 2349 256 2383
Fragments arranged by size:
2383 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4988
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4988
AvaI C'yCGr_G
Cuts at: 277 748 1517 1727 2008 277
Size: 471 769 210 281 3257
Fragments arranged by size:
3257 769 471 281 210
BamHI G'GATC_C
Cuts at: 2013 2013
Size: 4988
BanI G'GyrC_C
Cuts at: 198 499 1432 1438 1584 2004 2418 2666 2701
Size: 301 933 6 146 420 414 248 35
Cuts at: 2701 198
Size: 2485
Fragments arranged by size:
2485 933 420 414 301 248 146 35
6
BanII G_rGCy'C
Cuts at: 136 284 1123 1392 1767 1907 2002 3032 136
Size: 148 839 269 375 140 95 1030 2092
Fragments arranged by size:
2092 1030 839 375 269 148 140 95
BbeI G_GCGC'C
Cuts at: 2422 2670 2422
Size: 248 4740
BbsI GAAGACnn'nnnn_
Cuts at: 1150 1150
Size: 4988
BbvCI CC'TCA_GC
Cuts at: 272 272
Size: 4988
BciVI GTATCCnnnnn_n'
Cuts at: 2886 3647 4988 2886
Size: 761 1341 2886
Fragments arranged by size:
2886 1341 761
BclI T'GATC_A
Cuts at: 2508 2508
Size: 4988
BglI GCCn_nnn'nGGC
Cuts at: 1247 1247
Size: 4988
BglII A'GATC_T
Cuts at: 320 2503 4540 320
Size: 2183 2037 768
Fragments arranged by size:
2183 2037 768
BlpI GC'TnA_GC
Cuts at: 650 1117 2100 650
Size: 467 983 3538
Fragments arranged by size:
3538 983 467
Bme1580I G_kGCm'C
Cuts at: 136 177 203 504 644 947 1392 1589 1907
Size: 41 26 301 140 303 445 197 318
Cuts at: 1907 2613 2706 3762 136
Size: 706 93 1056 1362
Fragments arranged by size:
1362 1056 706 445 318 303 301 197
140 93 41 26
BmgBI CAC'GTC
Cuts at: 618 914 1422 618
Size: 296 508 4184
Fragments arranged by size:
4184 508 296
BmrI ACTGGGnnnn_n'
Cuts at: 1395 1973 2200 2281 2616 4760 1395
Size: 578 227 81 335 2144 1623
Fragments arranged by size:
2144 1623 578 335 227 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3355 3355
Size: 4988
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 244 352 694 808 1279 1901 244
Size: 108 342 114 471 622 3331
Fragments arranged by size:
3331 622 471 342 114 108
Bpu10I CC'TnA_GC
Cuts at: 178 272 178
Size: 94 4894
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 679 2164 3043 3535 3833 679
Size: 1485 879 492 298 1834
Fragments arranged by size:
1834 1485 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4692 4692
Size: 4988
BsaAI yAC'GTr
Cuts at: 2341 2341
Size: 4988
BsaBI GATnn'nnATC
Cuts at: 6 1855 2502 4539 6
Size: 1849 647 2037 455
Fragments arranged by size:
2037 1849 647 455
BsaHI Gr'CG_yC
Cuts at: 371 851 1514 2419 2667 3369 4865 371
Size: 480 663 905 248 702 1496 494
Fragments arranged by size:
1496 905 702 663 494 480 248
BsaWI w'CCGG_w
Cuts at: 995 1277 1313 1679 2177 2698 3376 3650 3797
Size: 282 36 366 498 521 678 274 147
Cuts at: 3797 4208 995
Size: 411 1775
Fragments arranged by size:
1775 678 521 498 411 366 282 274
147 36
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 241 582 1141 1231 241
Size: 341 559 90 3998
Fragments arranged by size:
3998 559 341 90
BseYI C'CCAG_C
Cuts at: 247 809 1270 1613 2413 3171 3748 4244 247
Size: 562 461 343 800 758 577 496 991
Fragments arranged by size:
991 800 758 577 562 496 461 343
BsiEI CG_ry'CG
Cuts at: 104 1801 2530 2576 3784 104
Size: 1697 729 46 1208 1308
Fragments arranged by size:
1697 1308 1208 729 46
BsiHKAI G_wGCw'C
Cuts at: 721 947 1367 1880 2002 2780 2970 3762 721
Size: 226 420 513 122 778 190 792 1947
Fragments arranged by size:
1947 792 778 513 420 226 190 122
BsmI GAATG_Cn'
Cuts at: 1656 1787 1656
Size: 131 4857
BsmBI CGTCTCn'nnnn_
Cuts at: 198 1021 1719 198
Size: 823 698 3467
Fragments arranged by size:
3467 823 698
Bsp1286I G_dGCh'C
Cuts at: 136 177 203 284 504 644 721 947 1123
Size: 41 26 81 220 140 77 226 176
Cuts at: 1123 1367 1392 1589 1767 1880 1907 2002 2613
Size: 244 25 197 178 113 27 95 611
Cuts at: 2613 2706 2780 2970 3032 3762 136
Size: 93 74 190 62 730 1362
Fragments arranged by size:
1362 730 611 244 226 220 197 190
178 176 140 113 95 93 81 77
74 62 41 27 26 25
BspEI T'CCGG_A
Cuts at: 1313 1679 4208 1313
Size: 366 2529 2093
Fragments arranged by size:
2529 2093 366
BspHI T'CATG_A
Cuts at: 4164 4164
Size: 4988
BspMI ACCTGCnnnn'nnnn_
Cuts at: 675 957 1207 1304 2038 2252 2554 2935 675
Size: 282 250 97 734 214 302 381 2728
Fragments arranged by size:
2728 734 381 302 282 250 214 97
BsrBI CCG'CTC
Cuts at: 50 663 3280 3334 4818 4983 50
Size: 613 2617 54 1484 165 55
Fragments arranged by size:
2617 1484 613 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1091 2900 1091
Size: 1809 3179
BsrFI r'CCGG_y
Cuts at: 1277 1435 1800 2986 3167 3376 1277
Size: 158 365 1186 181 209 2889
Fragments arranged by size:
2889 1186 365 209 181 158
BssSI C'ACGA_G
Cuts at: 605 1288 1621 3259 3617 605
Size: 683 333 1638 358 1976
Fragments arranged by size:
1976 1638 683 358 333
BstAPI GCAn_nnn'nTGC
Cuts at: 345 690 2365 345
Size: 345 1675 2968
Fragments arranged by size:
2968 1675 345
BstBI TT'CG_AA
Cuts at: 3349 3349
Size: 4988
BstEII G'GTnAC_C
Cuts at: 621 621
Size: 4988
BstXI CCAn_nnnn'nTGG
Cuts at: 969 1449 969
Size: 480 4508
BstYI r'GATC_y
Cuts at: 1 320 1316 2013 2480 2503 2838 3084 4096
Size: 319 996 697 467 23 335 246 1012
Cuts at: 4096 4182 4194 4540 1
Size: 86 12 346 449
Fragments arranged by size:
1012 996 697 467 449 346 335 319
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1124 1696 1124
Size: 572 4416
BtgI C'CryG_G
Cuts at: 143 557 878 1085 1736 143
Size: 414 321 207 651 3395
Fragments arranged by size:
3395 651 414 321 207
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2938 3119 3282 2938
Size: 181 163 4644
Fragments arranged by size:
4644 181 163
BtsI GCAGTG_nn'
Cuts at: 177 1560 1823 2231 177
Size: 1383 263 408 2934
Fragments arranged by size:
2934 1383 408 263
DraI TTT'AAA
Cuts at: 327 1987 327
Size: 1660 3328
DraIII CAC_nnn'GTG
Cuts at: 526 1623 1645 526
Size: 1097 22 3869
Fragments arranged by size:
3869 1097 22
DrdI GACnn_nn'nnGTC
Cuts at: 2694 3552 2694
Size: 858 4130
EaeI y'GGCC_r
Cuts at: 156 560 611 1088 1625 1673 1798 2573 2747
Size: 404 51 477 537 48 125 775 174
Cuts at: 2747 3138 3165 4510 156
Size: 391 27 1345 634
Fragments arranged by size:
1345 775 634 537 477 404 391 174
125 51 48 27
EagI C'GGCC_G
Cuts at: 1798 2573 1798
Size: 775 4213
EarI CTCTTCn'nnn_
Cuts at: 3011 3221 3011
Size: 210 4778
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3196 3506 3652 4798 3196
Size: 310 146 1146 3386
Fragments arranged by size:
3386 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2000 2000
Size: 4988
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 244 348 352 694 808 1162 1279 1901 2813
Size: 104 4 342 114 354 117 622 912
Cuts at: 2813 3245 3992 4260 244
Size: 432 747 268 972
Fragments arranged by size:
972 912 747 622 432 354 342 268
117 114 104 4
EcoNI CCTnn'n_nnAGG
Cuts at: 226 226
Size: 4988
EcoO109I rG'GnC_Cy
Cuts at: 132 268 480 1248 1293 1578 1903 1904 1936
Size: 136 212 768 45 285 325 1 32
Cuts at: 1936 1967 2127 132
Size: 31 160 2993
Fragments arranged by size:
2993 768 325 285 212 160 136 45
32 31 1
EcoRI G'AATT_C
Cuts at: 1992 1992
Size: 4988
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3007 3007
Size: 4988
FspI TGC'GCA
Cuts at: 1063 2769 1063
Size: 1706 3282
FspAI rTGC'GCAy
Cuts at: 1063 1063
Size: 4988
HaeII r_GCGC'y
Cuts at: 248 381 464 2422 2670 3692 4671 248
Size: 133 83 1958 248 1022 979 565
Fragments arranged by size:
1958 1022 979 565 248 133 83
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 807 839 2830 2860 2862 2892 807
Size: 32 1991 30 2 30 2903
Fragments arranged by size:
2903 1991 32 30 30 2
HincII GTy'rAC
Cuts at: 2027 2027
Size: 4988
KasI G'GCGC_C
Cuts at: 2418 2666 2418
Size: 248 4740
KpnI G_GTAC'C
Cuts at: 2008 2008
Size: 4988
MfeI C'AATT_G
Cuts at: 1393 1393
Size: 4988
MluI A'CGCG_T
Cuts at: 4421 4421
Size: 4988
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3659 3843 3659
Size: 184 4804
MscI TGG'CCA
Cuts at: 158 562 1090 1627 1675 2749 158
Size: 404 528 537 48 1074 2397
Fragments arranged by size:
2397 1074 537 528 404 48
MslI CAynn'nnrTG
Cuts at: 1594 1883 2977 3104 1594
Size: 289 1094 127 3478
Fragments arranged by size:
3478 1094 289 127
MspA1I CmG'CkG
Cuts at: 672 786 1053 2099 2413 2773 3786 4031 672
Size: 114 267 1046 314 360 1013 245 1629
Fragments arranged by size:
1629 1046 1013 360 314 267 245 114
NaeI GCC'GGC
Cuts at: 3169 3169
Size: 4988
NarI GG'CG_CC
Cuts at: 2419 2667 2419
Size: 248 4740
NcoI C'CATG_G
Cuts at: 143 557 1085 1736 143
Size: 414 528 651 3395
Fragments arranged by size:
3395 651 528 414
NgoMIV G'CCGG_C
Cuts at: 3167 3167
Size: 4988
NspI r_CATG'y
Cuts at: 1267 2041 2541 3448 1267
Size: 774 500 907 2807
Fragments arranged by size:
2807 907 774 500
PciI A'CATG_T
Cuts at: 3444 3444
Size: 4988
PflMI CCAn_nnn'nTGG
Cuts at: 1086 1086
Size: 4988
PfoI T'CCnGG_A
Cuts at: 983 1379 1631 983
Size: 396 252 4340
Fragments arranged by size:
4340 396 252
PmeI GTTT'AAAC
Cuts at: 1987 1987
Size: 4988
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4127 4159 4127
Size: 32 4956
PpuMI rG'GwC_Cy
Cuts at: 1293 1578 1936 1967 1293
Size: 285 358 31 4314
Fragments arranged by size:
4314 358 285 31
PspOMI G'GGCC_C
Cuts at: 132 1388 1903 132
Size: 1256 515 3217
Fragments arranged by size:
3217 1256 515
PspXI vC'TCGA_Gb
Cuts at: 748 748
Size: 4988
PstI C_TGCA'G
Cuts at: 210 341 404 468 695 1762 2035 2720 210
Size: 131 63 64 227 1067 273 685 2478
Fragments arranged by size:
2478 1067 685 273 227 131 64 63
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4988
PvuII CAG'CTG
Cuts at: 672 1053 2413 2773 672
Size: 381 1360 360 2887
Fragments arranged by size:
2887 1360 381 360
RsrII CG'GwC_CG
Cuts at: 3183 3183
Size: 4988
SacI G_AGCT'C
Cuts at: 2002 2002
Size: 4988
SalI G'TCGA_C
Cuts at: 2025 2025
Size: 4988
SapI GCTCTTCn'nnn_
Cuts at: 3011 3221 3011
Size: 210 4778
SbfI CC_TGCA'GG
Cuts at: 2035 2035
Size: 4988
ScaI AGT'ACT
Cuts at: 4505 4505
Size: 4988
SexAI A'CCwGG_T
Cuts at: 1580 1580
Size: 4988
SfcI C'TryA_G
Cuts at: 33 206 257 285 337 400 464 691 1758
Size: 173 51 28 52 63 64 227 1067
Cuts at: 1758 2031 2716 3709 3900 33
Size: 273 685 993 191 1121
Fragments arranged by size:
1121 1067 993 685 273 227 191 173
64 63 52 51 28
SfoI GGC'GCC
Cuts at: 2420 2668 2420
Size: 248 4740
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4988
SmaI CCC'GGG
Cuts at: 279 1519 1729 2010 279
Size: 1240 210 281 3257
Fragments arranged by size:
3257 1240 281 210
SmlI C'TyrA_G
Cuts at: 658 748 2143 3058 3550 3812 658
Size: 90 1395 915 492 262 1834
Fragments arranged by size:
1834 1395 915 492 262 90
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4988
SphI G_CATG'C
Cuts at: 2041 2541 2041
Size: 500 4488
SrfI GCCC'GGGC
Cuts at: 279 279
Size: 4988
StuI AGG'CCT
Cuts at: 775 979 775
Size: 204 4784
StyI C'CwwG_G
Cuts at: 143 545 557 704 1085 1661 1736 2122 2476
Size: 402 12 147 381 576 75 386 354
Cuts at: 2476 143
Size: 2655
Fragments arranged by size:
2655 576 402 386 381 354 147 75
12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2571 2704 3163 3372 2571
Size: 133 459 209 4187
Fragments arranged by size:
4187 459 209 133
TatI w'GTAC_w
Cuts at: 899 4503 899
Size: 3604 1384
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4505 4537 4505
Size: 32 4956
Tth111I GACn'n_nGTC
Cuts at: 2785 2785
Size: 4988
XbaI T'CTAG_A
Cuts at: 2019 2019
Size: 4988
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 966 1668 2483 966
Size: 702 815 3471
Fragments arranged by size:
3471 815 702
XhoI C'TCGA_G
Cuts at: 748 748
Size: 4988
XmaI C'CCGG_G
Cuts at: 277 1517 1727 2008 277
Size: 1240 210 281 3257
Fragments arranged by size:
3257 1240 281 210
XmnI GAAnn'nnTTC
Cuts at: 979 979
Size: 4988
ZraI GAC'GTC
Cuts at: 372 372
Size: 4988
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII
Hin4I HincII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PfoI PmeI PpiI PpuMI PspOMI PspXI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SrfI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AhdI AloI AscI AvrII BaeI BcgI BfrBI
BmtI BsaXI BsgI BsiWI BsrGI BssHII BstZ17I ClaI
CspCI EcoRV FseI HindIII HpaI NdeI NheI NotI
NruI NsiI PacI PasI PmlI PshAI PsiI PsrI
SacII SanDI SfiI SgrAI SnaBI SspI SwaI