Restriction map for pF1KE0343
 (Circular) (Six-base) MAPSORT of: pF1KE0343.seq  Check: 7139  from: 1  to: 4928



pF1KE0343  4928 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:13  ..

AccI GT'mk_AC

Cuts at:    305     341    1966     305 
   Size:         36    1625    3267

  Fragments arranged by size: 

                3267    1625      36
 
Acc65I G'GTAC_C

Cuts at:   1202    1944    1202 
   Size:        742    4186
 
AclI AA'CG_TT

Cuts at:   4768    4768 
   Size:       4928
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   2753    3185    3932    4200    2753 
   Size:        432     747     268    3481

  Fragments arranged by size: 

                3481     747     432     268
 
AfeI AGC'GCT

Cuts at:   1885    1885 
   Size:       4928
 
AflIII A'CryG_T

Cuts at:    653    1293    2280    3384    4361     653 
   Size:        640     987    1104     977    1220

  Fragments arranged by size: 

                1220    1104     987     977     640
 
AgeI A'CCGG_T

Cuts at:   3316    3316 
   Size:       4928
 
AhdI GACnn_n'nnGTC

Cuts at:   1845    1845 
   Size:       4928
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    986     989     986 
   Size:          3    4925
 
AlwNI CAG_nnn'CTG

Cuts at:   1024    1065    1356    1598    1620    2233    3800    1024 
   Size:         41     291     242      22     613    1567    2152

  Fragments arranged by size: 

                2152    1567     613     291     242      41      22
 
ApaI G_GGCC'C

Cuts at:   1017    1017 
   Size:       4928
 
ApaLI G'TGCA_C

Cuts at:   3698    3698 
   Size:       4928
 
ApoI r'AATT_y

Cuts at:   1932    4281    4537    1932 
   Size:       2349     256    2323

  Fragments arranged by size: 

                2349    2323     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4928
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4928
 
AvaI C'yCGr_G

Cuts at:   1447    1819    1948    1447 
   Size:        372     129    4427

  Fragments arranged by size: 

                4427     372     129
 
BamHI G'GATC_C

Cuts at:   1953    1953 
   Size:       4928
 
BanI G'GyrC_C

Cuts at:   1202    1944    2358    2606    2641    1202 
   Size:        742     414     248      35    3489

  Fragments arranged by size: 

                3489     742     414     248      35
 
BanII G_rGCy'C

Cuts at:    295     805     944     961    1017    1448    1538    1942    2972 
   Size:        510     139      17      56     431      90     404    1030

Cuts at:   2972     295 
   Size:       2251

  Fragments arranged by size: 

                2251    1030     510     431     404     139      90      56
                  17
 
BbeI G_GCGC'C

Cuts at:   2362    2610    2362 
   Size:        248    4680
 
BbsI GAAGACnn'nnnn_

Cuts at:   1403    1403 
   Size:       4928
 
BciVI GTATCCnnnnn_n'

Cuts at:   2826    3587    4928    2826 
   Size:        761    1341    2826

  Fragments arranged by size: 

                2826    1341     761
 
BclI T'GATC_A

Cuts at:    513    2448     513 
   Size:       1935    2993
 
BglI GCCn_nnn'nGGC

Cuts at:    565     928     565 
   Size:        363    4565
 
BglII A'GATC_T

Cuts at:    224    2443    4480     224 
   Size:       2219    2037     672

  Fragments arranged by size: 

                2219    2037     672
 
BlpI GC'TnA_GC

Cuts at:   1346    1579    2040    1346 
   Size:        233     461    4234

  Fragments arranged by size: 

                4234     461     233
 
Bme1580I G_kGCm'C

Cuts at:   1017    1127    1649    2553    2646    3702    1017 
   Size:        110     522     904      93    1056    2243

  Fragments arranged by size: 

                2243    1056     904     522     110      93
 
BmgBI CAC'GTC

Cuts at:    117     183     117 
   Size:         66    4862
 
BmrI ACTGGGnnnn_n'

Cuts at:    180     518    1608    2140    2221    2556    4700     180 
   Size:        338    1090     532      81     335    2144     408

  Fragments arranged by size: 

                2144    1090     532     408     338     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3295    3295 
   Size:       4928
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    229     830     958    1031    1402    1609    1846     229 
   Size:        601     128      73     371     207     237    3311

  Fragments arranged by size: 

                3311     601     371     237     207     128      73
 
Bpu10I CC'TnA_GC

Cuts at:   1501    1501 
   Size:       4928
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    490    2104    2983    3475    3773     490 
   Size:       1614     879     492     298    1645

  Fragments arranged by size: 

                1645    1614     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   1686    4632    1686 
   Size:       2946    1982
 
BsaAI yAC'GTr

Cuts at:    656    1296    2281     656 
   Size:        640     985    3303

  Fragments arranged by size: 

                3303     985     640
 
BsaBI GATnn'nnATC

Cuts at:      6    2442    4479       6 
   Size:       2436    2037     455

  Fragments arranged by size: 

                2436    2037     455
 
BsaHI Gr'CG_yC

Cuts at:    520    2359    2607    3309    4805     520 
   Size:       1839     248     702    1496     643

  Fragments arranged by size: 

                1839    1496     702     643     248
 
BsaWI w'CCGG_w

Cuts at:   1319    2117    2638    3316    3590    3737    4148    1319 
   Size:        798     521     678     274     147     411    2099

  Fragments arranged by size: 

                2099     798     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1267    1297    1267 
   Size:         30    4898
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    226     784     907    1153    1347    1384    1447    1525     226 
   Size:        558     123     246     194      37      63      78    3629

  Fragments arranged by size: 

                3629     558     246     194     123      78      63      37
            
 
BseYI C'CCAG_C

Cuts at:    191     195     810    1694    1811    1916    2353    3111    3688 
   Size:          4     615     884     117     105     437     758     577

Cuts at:   3688    4184     191 
   Size:        496     935

  Fragments arranged by size: 

                 935     884     758     615     577     496     437     117
                 105       4
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    418     816    1633     418 
   Size:        398     817    3713

  Fragments arranged by size: 

                3713     817     398
 
BsiEI CG_ry'CG

Cuts at:    104    2470    2516    3724     104 
   Size:       2366      46    1208    1308

  Fragments arranged by size: 

                2366    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:   1538    1942    2720    2910    3702    1538 
   Size:        404     778     190     792    2764

  Fragments arranged by size: 

                2764     792     778     404     190
 
Bsp1286I G_dGCh'C

Cuts at:    295     805     944     961    1017    1127    1448    1538    1649 
   Size:        510     139      17      56     110     321      90     111

Cuts at:   1649    1942    2553    2646    2720    2910    2972    3702     295 
   Size:        293     611      93      74     190      62     730    1521

  Fragments arranged by size: 

                1521     730     611     510     321     293     190     139
                 111     110      93      90      74      62      56      17
            
 
BspEI T'CCGG_A

Cuts at:   1319    4148    1319 
   Size:       2829    2099
 
BspHI T'CATG_A

Cuts at:   4104    4104 
   Size:       4928
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1978    2192    2494    2875    1978 
   Size:        214     302     381    4031

  Fragments arranged by size: 

                4031     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     934    1005    3220    3274    4758    4923      50 
   Size:        884      71    2215      54    1484     165      55

  Fragments arranged by size: 

                2215    1484     884     165      71      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2840    2840 
   Size:       4928
 
BsrFI r'CCGG_y

Cuts at:   1740    2926    3107    3316    1740 
   Size:       1186     181     209    3352

  Fragments arranged by size: 

                3352    1186     209     181
 
BsrGI T'GTAC_A

Cuts at:    602     602 
   Size:       4928
 
BssHII G'CGCG_C

Cuts at:    124     124 
   Size:       4928
 
BssSI C'ACGA_G

Cuts at:   3199    3557    3199 
   Size:        358    4570
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1225    1356    1620    1644    2305    1225 
   Size:        131     264      24     661    3848

  Fragments arranged by size: 

                3848     661     264     131      24
 
BstBI TT'CG_AA

Cuts at:   1521    3289    1521 
   Size:       1768    3160
 
BstXI CCAn_nnnn'nTGG

Cuts at:    561    1055    1666     561 
   Size:        494     611    3823

  Fragments arranged by size: 

                3823     611     494
 
BstYI r'GATC_y

Cuts at:      1     224     501    1953    2420    2443    2778    3024    4036 
   Size:        223     277    1452     467      23     335     246    1012

Cuts at:   4036    4122    4134    4480       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1452    1012     467     449     346     335     277     246
                 223      86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:    911    1430     911 
   Size:        519    4409
 
BtgI C'CryG_G

Cuts at:    107     554     710    1835    1910     107 
   Size:        447     156    1125      75    3125

  Fragments arranged by size: 

                3125    1125     447     156      75
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1527    2878    3059    3222    1527 
   Size:       1351     181     163    3233

  Fragments arranged by size: 

                3233    1351     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1357    1493    2171    1357 
   Size:        136     678    4114

  Fragments arranged by size: 

                4114     678     136
 
DraI TTT'AAA

Cuts at:   1927    1927 
   Size:       4928
 
DrdI GACnn_nn'nnGTC

Cuts at:   1276    1678    2634    3492    1276 
   Size:        402     956     858    2712

  Fragments arranged by size: 

                2712     956     858     402
 
EaeI y'GGCC_r

Cuts at:    626    1734    1784    1832    2513    2687    3078    3105    4450 
   Size:       1108      50      48     681     174     391      27    1345

Cuts at:   4450     626 
   Size:       1104

  Fragments arranged by size: 

                1345    1108    1104     681     391     174      50      48
                  27
 
EagI C'GGCC_G

Cuts at:   2513    2513 
   Size:       4928
 
EarI CTCTTCn'nnn_

Cuts at:    396    1543    2951    3161     396 
   Size:       1147    1408     210    2163

  Fragments arranged by size: 

                2163    1408    1147     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   3136    3446    3592    4738    3136 
   Size:        310     146    1146    3326

  Fragments arranged by size: 

                3326    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1536    1940    1536 
   Size:        404    4524
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    229     830     958    1031    1402    1609    1846    2753    3185 
   Size:        601     128      73     371     207     237     907     432

Cuts at:   3185    3932    4200     229 
   Size:        747     268     957

  Fragments arranged by size: 

                 957     907     747     601     432     371     268     237
                 207     128      73
 
EcoO109I rG'GnC_Cy

Cuts at:    348     435     531     621     677     743     838    1013    1014 
   Size:         87      96      90      56      66      95     175       1

Cuts at:   1014    1278    2067     348 
   Size:        264     789    3209

  Fragments arranged by size: 

                3209     789     264     175      96      95      90      87
                  66      56       1
 
EcoRI G'AATT_C

Cuts at:   1932    1932 
   Size:       4928
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2947    2947 
   Size:       4928
 
FspI TGC'GCA

Cuts at:   2709    2709 
   Size:       4928
 
HaeII r_GCGC'y

Cuts at:   1175    1887    2362    2610    3632    4611    1175 
   Size:        712     475     248    1022     979    1492

  Fragments arranged by size: 

                1492    1022     979     712     475     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    346     378     448     480     688     720    1012    1044    1831 
   Size:         32      70      32     208      32     292      32     787

Cuts at:   1831    1863    2770    2800    2802    2832     346 
   Size:         32     907      30       2      30    2442

  Fragments arranged by size: 

                2442     907     787     292     208      70      32      32
                  32      32      32      30      30       2
 
HincII GTy'rAC

Cuts at:   1967    1967 
   Size:       4928
 
KasI G'GCGC_C

Cuts at:   2358    2606    2358 
   Size:        248    4680
 
KpnI G_GTAC'C

Cuts at:   1206    1948    1206 
   Size:        742    4186
 
MluI A'CGCG_T

Cuts at:   4361    4361 
   Size:       4928
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    668    1289    3599    3783     668 
   Size:        621    2310     184    1813

  Fragments arranged by size: 

                2310    1813     621     184
 
MscI TGG'CCA

Cuts at:    628    1736    1786    2689     628 
   Size:       1108      50     903    2867

  Fragments arranged by size: 

                2867    1108     903      50
 
MslI CAynn'nnrTG

Cuts at:   1664    2917    3044    1664 
   Size:       1253     127    3548

  Fragments arranged by size: 

                3548    1253     127
 
MspA1I CmG'CkG

Cuts at:    195     721    1474    1677    1782    2039    2353    2713    3726 
   Size:        526     753     203     105     257     314     360    1013

Cuts at:   3726    3971     195 
   Size:        245    1152

  Fragments arranged by size: 

                1152    1013     753     526     360     314     257     245
                 203     105
 
NaeI GCC'GGC

Cuts at:   1742    3109    1742 
   Size:       1367    3561
 
NarI GG'CG_CC

Cuts at:   2359    2607    2359 
   Size:        248    4680
 
NcoI C'CATG_G

Cuts at:    107     554     710    1910     107 
   Size:        447     156    1200    3125

  Fragments arranged by size: 

                3125    1200     447     156
 
NgoMIV G'CCGG_C

Cuts at:   1740    3107    1740 
   Size:       1367    3561
 
NspI r_CATG'y

Cuts at:    705     996    1645    1981    2481    3388     705 
   Size:        291     649     336     500     907    2245

  Fragments arranged by size: 

                2245     907     649     500     336     291
 
PasI CC'CwG_GG

Cuts at:    864    1463     864 
   Size:        599    4329
 
PciI A'CATG_T

Cuts at:   3384    3384 
   Size:       4928
 
PflMI CCAn_nnn'nTGG

Cuts at:    170     170 
   Size:       4928
 
PfoI T'CCnGG_A

Cuts at:    487     952    1565    1625     487 
   Size:        465     613      60    3790

  Fragments arranged by size: 

                3790     613     465      60
 
PmeI GTTT'AAAC

Cuts at:   1927    1927 
   Size:       4928
 
PmlI CAC'GTG

Cuts at:    656    1296     656 
   Size:        640    4288
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    442     474    4067    4099     442 
   Size:         32    3593      32    1271

  Fragments arranged by size: 

                3593    1271      32      32
 
PpuMI rG'GwC_Cy

Cuts at:    435     531     621     743    1278     435 
   Size:         96      90     122     535    4085

  Fragments arranged by size: 

                4085     535     122      96      90
 
PspOMI G'GGCC_C

Cuts at:   1013    1013 
   Size:       4928
 
PstI C_TGCA'G

Cuts at:   1187    1331    1368    1781    1975    2660    1187 
   Size:        144      37     413     194     685    3455

  Fragments arranged by size: 

                3455     685     413     194     144      37
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4928
 
PvuII CAG'CTG

Cuts at:    195    1677    1782    2353    2713     195 
   Size:       1482     105     571     360    2410

  Fragments arranged by size: 

                2410    1482     571     360     105
 
RsrII CG'GwC_CG

Cuts at:   3123    3123 
   Size:       4928
 
SacI G_AGCT'C

Cuts at:   1538    1942    1538 
   Size:        404    4524
 
SalI G'TCGA_C

Cuts at:   1965    1965 
   Size:       4928
 
SanDI GG'GwC_CC

Cuts at:    435     743     435 
   Size:        308    4620
 
SapI GCTCTTCn'nnn_

Cuts at:   1543    2951    3161    1543 
   Size:       1408     210    3310

  Fragments arranged by size: 

                3310    1408     210
 
SbfI CC_TGCA'GG

Cuts at:   1975    1975 
   Size:       4928
 
ScaI AGT'ACT

Cuts at:   4445    4445 
   Size:       4928
 
SfcI C'TryA_G

Cuts at:     33     306    1183    1327    1364    1777    1971    2656    3649 
   Size:        273     877     144      37     413     194     685     993

Cuts at:   3649    3840      33 
   Size:        191    1121

  Fragments arranged by size: 

                1121     993     877     685     413     273     194     191
                 144      37
 
SfiI GGCCn_nnn'nGGCC

Cuts at:    565     928     565 
   Size:        363    4565
 
SfoI GGC'GCC

Cuts at:   2360    2608    2360 
   Size:        248    4680
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4928
 
SmaI CCC'GGG

Cuts at:   1821    1950    1821 
   Size:        129    4799
 
SmlI C'TyrA_G

Cuts at:    505    2083    2998    3490    3752     505 
   Size:       1578     915     492     262    1681

  Fragments arranged by size: 

                1681    1578     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4928
 
SphI G_CATG'C

Cuts at:   1981    2481    1981 
   Size:        500    4428
 
SrfI GCCC'GGGC

Cuts at:   1821    1821 
   Size:       4928
 
StuI AGG'CCT

Cuts at:    916    1113     916 
   Size:        197    4731
 
StyI C'CwwG_G

Cuts at:    107     554     710     926     944    1082    1910    2062    2416 
   Size:        447     156     216      18     138     828     152     354

Cuts at:   2416     107 
   Size:       2619

  Fragments arranged by size: 

                2619     828     447     354     216     156     152     138
                  18
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    856    2511    2644    3103    3312     856 
   Size:       1655     133     459     209    2472

  Fragments arranged by size: 

                2472    1655     459     209     133
 
TatI w'GTAC_w

Cuts at:    602    1283    1436    4443     602 
   Size:        681     153    3007    1087

  Fragments arranged by size: 

                3007    1087     681     153
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    645     677    4445    4477     645 
   Size:         32    3768      32    1096

  Fragments arranged by size: 

                3768    1096      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   2725    2725 
   Size:       4928
 
XbaI T'CTAG_A

Cuts at:   1422    1959    1422 
   Size:        537    4391
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    114     561     933    2423     114 
   Size:        447     372    1490    2619

  Fragments arranged by size: 

                2619    1490     447     372
 
XmaI C'CCGG_G

Cuts at:   1819    1948    1819 
   Size:        129    4799
 
XmnI GAAnn'nnTTC

Cuts at:    457     640     457 
   Size:        183    4745
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI     AhdI
     AlfI    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI
    BamHI     BanI    BanII     BbeI     BbsI    BciVI     BclI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI     DraI     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI
     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     NspI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI   PspOMI     PstI     PvuI    PvuII    RsrII     SacI     SalI
    SanDI     SapI     SbfI     ScaI     SfcI     SfiI     SfoI     SgfI
     SmaI     SmlI     SpeI     SphI     SrfI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII    AflII     AleI     AloI     AscI    AvrII     BaeI
    BbvCI     BcgI    BfrBI     BmtI    BsiWI     BsmI    BsmBI   BstEII
  BstZ17I     ClaI    CspCI   DraIII    EcoNI    EcoRV     FseI    FspAI
  HindIII     HpaI     MfeI     NdeI     NheI     NotI     NruI     NsiI
     PacI    PshAI     PsiI    PspXI     PsrI    SacII    SexAI    SgrAI
    SnaBI     SspI     SwaI     XhoI     ZraI
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