Restriction map for
pF1KE0343
(Circular) (Six-base) MAPSORT of: pF1KE0343.seq Check: 7139 from: 1 to: 4928
pF1KE0343 4928 bp
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AccI GT'mk_AC
Cuts at: 305 341 1966 305
Size: 36 1625 3267
Fragments arranged by size:
3267 1625 36
Acc65I G'GTAC_C
Cuts at: 1202 1944 1202
Size: 742 4186
AclI AA'CG_TT
Cuts at: 4768 4768
Size: 4928
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2753 3185 3932 4200 2753
Size: 432 747 268 3481
Fragments arranged by size:
3481 747 432 268
AfeI AGC'GCT
Cuts at: 1885 1885
Size: 4928
AflIII A'CryG_T
Cuts at: 653 1293 2280 3384 4361 653
Size: 640 987 1104 977 1220
Fragments arranged by size:
1220 1104 987 977 640
AgeI A'CCGG_T
Cuts at: 3316 3316
Size: 4928
AhdI GACnn_n'nnGTC
Cuts at: 1845 1845
Size: 4928
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 986 989 986
Size: 3 4925
AlwNI CAG_nnn'CTG
Cuts at: 1024 1065 1356 1598 1620 2233 3800 1024
Size: 41 291 242 22 613 1567 2152
Fragments arranged by size:
2152 1567 613 291 242 41 22
ApaI G_GGCC'C
Cuts at: 1017 1017
Size: 4928
ApaLI G'TGCA_C
Cuts at: 3698 3698
Size: 4928
ApoI r'AATT_y
Cuts at: 1932 4281 4537 1932
Size: 2349 256 2323
Fragments arranged by size:
2349 2323 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4928
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4928
AvaI C'yCGr_G
Cuts at: 1447 1819 1948 1447
Size: 372 129 4427
Fragments arranged by size:
4427 372 129
BamHI G'GATC_C
Cuts at: 1953 1953
Size: 4928
BanI G'GyrC_C
Cuts at: 1202 1944 2358 2606 2641 1202
Size: 742 414 248 35 3489
Fragments arranged by size:
3489 742 414 248 35
BanII G_rGCy'C
Cuts at: 295 805 944 961 1017 1448 1538 1942 2972
Size: 510 139 17 56 431 90 404 1030
Cuts at: 2972 295
Size: 2251
Fragments arranged by size:
2251 1030 510 431 404 139 90 56
17
BbeI G_GCGC'C
Cuts at: 2362 2610 2362
Size: 248 4680
BbsI GAAGACnn'nnnn_
Cuts at: 1403 1403
Size: 4928
BciVI GTATCCnnnnn_n'
Cuts at: 2826 3587 4928 2826
Size: 761 1341 2826
Fragments arranged by size:
2826 1341 761
BclI T'GATC_A
Cuts at: 513 2448 513
Size: 1935 2993
BglI GCCn_nnn'nGGC
Cuts at: 565 928 565
Size: 363 4565
BglII A'GATC_T
Cuts at: 224 2443 4480 224
Size: 2219 2037 672
Fragments arranged by size:
2219 2037 672
BlpI GC'TnA_GC
Cuts at: 1346 1579 2040 1346
Size: 233 461 4234
Fragments arranged by size:
4234 461 233
Bme1580I G_kGCm'C
Cuts at: 1017 1127 1649 2553 2646 3702 1017
Size: 110 522 904 93 1056 2243
Fragments arranged by size:
2243 1056 904 522 110 93
BmgBI CAC'GTC
Cuts at: 117 183 117
Size: 66 4862
BmrI ACTGGGnnnn_n'
Cuts at: 180 518 1608 2140 2221 2556 4700 180
Size: 338 1090 532 81 335 2144 408
Fragments arranged by size:
2144 1090 532 408 338 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3295 3295
Size: 4928
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 229 830 958 1031 1402 1609 1846 229
Size: 601 128 73 371 207 237 3311
Fragments arranged by size:
3311 601 371 237 207 128 73
Bpu10I CC'TnA_GC
Cuts at: 1501 1501
Size: 4928
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 490 2104 2983 3475 3773 490
Size: 1614 879 492 298 1645
Fragments arranged by size:
1645 1614 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1686 4632 1686
Size: 2946 1982
BsaAI yAC'GTr
Cuts at: 656 1296 2281 656
Size: 640 985 3303
Fragments arranged by size:
3303 985 640
BsaBI GATnn'nnATC
Cuts at: 6 2442 4479 6
Size: 2436 2037 455
Fragments arranged by size:
2436 2037 455
BsaHI Gr'CG_yC
Cuts at: 520 2359 2607 3309 4805 520
Size: 1839 248 702 1496 643
Fragments arranged by size:
1839 1496 702 643 248
BsaWI w'CCGG_w
Cuts at: 1319 2117 2638 3316 3590 3737 4148 1319
Size: 798 521 678 274 147 411 2099
Fragments arranged by size:
2099 798 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1267 1297 1267
Size: 30 4898
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 226 784 907 1153 1347 1384 1447 1525 226
Size: 558 123 246 194 37 63 78 3629
Fragments arranged by size:
3629 558 246 194 123 78 63 37
BseYI C'CCAG_C
Cuts at: 191 195 810 1694 1811 1916 2353 3111 3688
Size: 4 615 884 117 105 437 758 577
Cuts at: 3688 4184 191
Size: 496 935
Fragments arranged by size:
935 884 758 615 577 496 437 117
105 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 418 816 1633 418
Size: 398 817 3713
Fragments arranged by size:
3713 817 398
BsiEI CG_ry'CG
Cuts at: 104 2470 2516 3724 104
Size: 2366 46 1208 1308
Fragments arranged by size:
2366 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1538 1942 2720 2910 3702 1538
Size: 404 778 190 792 2764
Fragments arranged by size:
2764 792 778 404 190
Bsp1286I G_dGCh'C
Cuts at: 295 805 944 961 1017 1127 1448 1538 1649
Size: 510 139 17 56 110 321 90 111
Cuts at: 1649 1942 2553 2646 2720 2910 2972 3702 295
Size: 293 611 93 74 190 62 730 1521
Fragments arranged by size:
1521 730 611 510 321 293 190 139
111 110 93 90 74 62 56 17
BspEI T'CCGG_A
Cuts at: 1319 4148 1319
Size: 2829 2099
BspHI T'CATG_A
Cuts at: 4104 4104
Size: 4928
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1978 2192 2494 2875 1978
Size: 214 302 381 4031
Fragments arranged by size:
4031 381 302 214
BsrBI CCG'CTC
Cuts at: 50 934 1005 3220 3274 4758 4923 50
Size: 884 71 2215 54 1484 165 55
Fragments arranged by size:
2215 1484 884 165 71 55 54
BsrDI GCAATG_nn'
Cuts at: 2840 2840
Size: 4928
BsrFI r'CCGG_y
Cuts at: 1740 2926 3107 3316 1740
Size: 1186 181 209 3352
Fragments arranged by size:
3352 1186 209 181
BsrGI T'GTAC_A
Cuts at: 602 602
Size: 4928
BssHII G'CGCG_C
Cuts at: 124 124
Size: 4928
BssSI C'ACGA_G
Cuts at: 3199 3557 3199
Size: 358 4570
BstAPI GCAn_nnn'nTGC
Cuts at: 1225 1356 1620 1644 2305 1225
Size: 131 264 24 661 3848
Fragments arranged by size:
3848 661 264 131 24
BstBI TT'CG_AA
Cuts at: 1521 3289 1521
Size: 1768 3160
BstXI CCAn_nnnn'nTGG
Cuts at: 561 1055 1666 561
Size: 494 611 3823
Fragments arranged by size:
3823 611 494
BstYI r'GATC_y
Cuts at: 1 224 501 1953 2420 2443 2778 3024 4036
Size: 223 277 1452 467 23 335 246 1012
Cuts at: 4036 4122 4134 4480 1
Size: 86 12 346 449
Fragments arranged by size:
1452 1012 467 449 346 335 277 246
223 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 911 1430 911
Size: 519 4409
BtgI C'CryG_G
Cuts at: 107 554 710 1835 1910 107
Size: 447 156 1125 75 3125
Fragments arranged by size:
3125 1125 447 156 75
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1527 2878 3059 3222 1527
Size: 1351 181 163 3233
Fragments arranged by size:
3233 1351 181 163
BtsI GCAGTG_nn'
Cuts at: 1357 1493 2171 1357
Size: 136 678 4114
Fragments arranged by size:
4114 678 136
DraI TTT'AAA
Cuts at: 1927 1927
Size: 4928
DrdI GACnn_nn'nnGTC
Cuts at: 1276 1678 2634 3492 1276
Size: 402 956 858 2712
Fragments arranged by size:
2712 956 858 402
EaeI y'GGCC_r
Cuts at: 626 1734 1784 1832 2513 2687 3078 3105 4450
Size: 1108 50 48 681 174 391 27 1345
Cuts at: 4450 626
Size: 1104
Fragments arranged by size:
1345 1108 1104 681 391 174 50 48
27
EagI C'GGCC_G
Cuts at: 2513 2513
Size: 4928
EarI CTCTTCn'nnn_
Cuts at: 396 1543 2951 3161 396
Size: 1147 1408 210 2163
Fragments arranged by size:
2163 1408 1147 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3136 3446 3592 4738 3136
Size: 310 146 1146 3326
Fragments arranged by size:
3326 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1536 1940 1536
Size: 404 4524
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 229 830 958 1031 1402 1609 1846 2753 3185
Size: 601 128 73 371 207 237 907 432
Cuts at: 3185 3932 4200 229
Size: 747 268 957
Fragments arranged by size:
957 907 747 601 432 371 268 237
207 128 73
EcoO109I rG'GnC_Cy
Cuts at: 348 435 531 621 677 743 838 1013 1014
Size: 87 96 90 56 66 95 175 1
Cuts at: 1014 1278 2067 348
Size: 264 789 3209
Fragments arranged by size:
3209 789 264 175 96 95 90 87
66 56 1
EcoRI G'AATT_C
Cuts at: 1932 1932
Size: 4928
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2947 2947
Size: 4928
FspI TGC'GCA
Cuts at: 2709 2709
Size: 4928
HaeII r_GCGC'y
Cuts at: 1175 1887 2362 2610 3632 4611 1175
Size: 712 475 248 1022 979 1492
Fragments arranged by size:
1492 1022 979 712 475 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 346 378 448 480 688 720 1012 1044 1831
Size: 32 70 32 208 32 292 32 787
Cuts at: 1831 1863 2770 2800 2802 2832 346
Size: 32 907 30 2 30 2442
Fragments arranged by size:
2442 907 787 292 208 70 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1967 1967
Size: 4928
KasI G'GCGC_C
Cuts at: 2358 2606 2358
Size: 248 4680
KpnI G_GTAC'C
Cuts at: 1206 1948 1206
Size: 742 4186
MluI A'CGCG_T
Cuts at: 4361 4361
Size: 4928
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 668 1289 3599 3783 668
Size: 621 2310 184 1813
Fragments arranged by size:
2310 1813 621 184
MscI TGG'CCA
Cuts at: 628 1736 1786 2689 628
Size: 1108 50 903 2867
Fragments arranged by size:
2867 1108 903 50
MslI CAynn'nnrTG
Cuts at: 1664 2917 3044 1664
Size: 1253 127 3548
Fragments arranged by size:
3548 1253 127
MspA1I CmG'CkG
Cuts at: 195 721 1474 1677 1782 2039 2353 2713 3726
Size: 526 753 203 105 257 314 360 1013
Cuts at: 3726 3971 195
Size: 245 1152
Fragments arranged by size:
1152 1013 753 526 360 314 257 245
203 105
NaeI GCC'GGC
Cuts at: 1742 3109 1742
Size: 1367 3561
NarI GG'CG_CC
Cuts at: 2359 2607 2359
Size: 248 4680
NcoI C'CATG_G
Cuts at: 107 554 710 1910 107
Size: 447 156 1200 3125
Fragments arranged by size:
3125 1200 447 156
NgoMIV G'CCGG_C
Cuts at: 1740 3107 1740
Size: 1367 3561
NspI r_CATG'y
Cuts at: 705 996 1645 1981 2481 3388 705
Size: 291 649 336 500 907 2245
Fragments arranged by size:
2245 907 649 500 336 291
PasI CC'CwG_GG
Cuts at: 864 1463 864
Size: 599 4329
PciI A'CATG_T
Cuts at: 3384 3384
Size: 4928
PflMI CCAn_nnn'nTGG
Cuts at: 170 170
Size: 4928
PfoI T'CCnGG_A
Cuts at: 487 952 1565 1625 487
Size: 465 613 60 3790
Fragments arranged by size:
3790 613 465 60
PmeI GTTT'AAAC
Cuts at: 1927 1927
Size: 4928
PmlI CAC'GTG
Cuts at: 656 1296 656
Size: 640 4288
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 442 474 4067 4099 442
Size: 32 3593 32 1271
Fragments arranged by size:
3593 1271 32 32
PpuMI rG'GwC_Cy
Cuts at: 435 531 621 743 1278 435
Size: 96 90 122 535 4085
Fragments arranged by size:
4085 535 122 96 90
PspOMI G'GGCC_C
Cuts at: 1013 1013
Size: 4928
PstI C_TGCA'G
Cuts at: 1187 1331 1368 1781 1975 2660 1187
Size: 144 37 413 194 685 3455
Fragments arranged by size:
3455 685 413 194 144 37
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4928
PvuII CAG'CTG
Cuts at: 195 1677 1782 2353 2713 195
Size: 1482 105 571 360 2410
Fragments arranged by size:
2410 1482 571 360 105
RsrII CG'GwC_CG
Cuts at: 3123 3123
Size: 4928
SacI G_AGCT'C
Cuts at: 1538 1942 1538
Size: 404 4524
SalI G'TCGA_C
Cuts at: 1965 1965
Size: 4928
SanDI GG'GwC_CC
Cuts at: 435 743 435
Size: 308 4620
SapI GCTCTTCn'nnn_
Cuts at: 1543 2951 3161 1543
Size: 1408 210 3310
Fragments arranged by size:
3310 1408 210
SbfI CC_TGCA'GG
Cuts at: 1975 1975
Size: 4928
ScaI AGT'ACT
Cuts at: 4445 4445
Size: 4928
SfcI C'TryA_G
Cuts at: 33 306 1183 1327 1364 1777 1971 2656 3649
Size: 273 877 144 37 413 194 685 993
Cuts at: 3649 3840 33
Size: 191 1121
Fragments arranged by size:
1121 993 877 685 413 273 194 191
144 37
SfiI GGCCn_nnn'nGGCC
Cuts at: 565 928 565
Size: 363 4565
SfoI GGC'GCC
Cuts at: 2360 2608 2360
Size: 248 4680
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4928
SmaI CCC'GGG
Cuts at: 1821 1950 1821
Size: 129 4799
SmlI C'TyrA_G
Cuts at: 505 2083 2998 3490 3752 505
Size: 1578 915 492 262 1681
Fragments arranged by size:
1681 1578 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4928
SphI G_CATG'C
Cuts at: 1981 2481 1981
Size: 500 4428
SrfI GCCC'GGGC
Cuts at: 1821 1821
Size: 4928
StuI AGG'CCT
Cuts at: 916 1113 916
Size: 197 4731
StyI C'CwwG_G
Cuts at: 107 554 710 926 944 1082 1910 2062 2416
Size: 447 156 216 18 138 828 152 354
Cuts at: 2416 107
Size: 2619
Fragments arranged by size:
2619 828 447 354 216 156 152 138
18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 856 2511 2644 3103 3312 856
Size: 1655 133 459 209 2472
Fragments arranged by size:
2472 1655 459 209 133
TatI w'GTAC_w
Cuts at: 602 1283 1436 4443 602
Size: 681 153 3007 1087
Fragments arranged by size:
3007 1087 681 153
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 645 677 4445 4477 645
Size: 32 3768 32 1096
Fragments arranged by size:
3768 1096 32 32
Tth111I GACn'n_nGTC
Cuts at: 2725 2725
Size: 4928
XbaI T'CTAG_A
Cuts at: 1422 1959 1422
Size: 537 4391
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 561 933 2423 114
Size: 447 372 1490 2619
Fragments arranged by size:
2619 1490 447 372
XmaI C'CCGG_G
Cuts at: 1819 1948 1819
Size: 129 4799
XmnI GAAnn'nnTTC
Cuts at: 457 640 457
Size: 183 4745
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SmaI SmlI SpeI SphI SrfI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AleI AloI AscI AvrII BaeI
BbvCI BcgI BfrBI BmtI BsiWI BsmI BsmBI BstEII
BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PshAI PsiI PspXI PsrI SacII SexAI SgrAI
SnaBI SspI SwaI XhoI ZraI