(Circular) (Six-base) MAPSORT of: pF1KE0343.seq Check: 7139 from: 1 to: 4928 pF1KE0343 4928 bp With 182 enzymes: SgfI * April 24, 2009 13:13 .. AccI GT'mk_AC Cuts at: 305 341 1966 305 Size: 36 1625 3267 Fragments arranged by size: 3267 1625 36 Acc65I G'GTAC_C Cuts at: 1202 1944 1202 Size: 742 4186 AclI AA'CG_TT Cuts at: 4768 4768 Size: 4928 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 2753 3185 3932 4200 2753 Size: 432 747 268 3481 Fragments arranged by size: 3481 747 432 268 AfeI AGC'GCT Cuts at: 1885 1885 Size: 4928 AflIII A'CryG_T Cuts at: 653 1293 2280 3384 4361 653 Size: 640 987 1104 977 1220 Fragments arranged by size: 1220 1104 987 977 640 AgeI A'CCGG_T Cuts at: 3316 3316 Size: 4928 AhdI GACnn_n'nnGTC Cuts at: 1845 1845 Size: 4928 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 986 989 986 Size: 3 4925 AlwNI CAG_nnn'CTG Cuts at: 1024 1065 1356 1598 1620 2233 3800 1024 Size: 41 291 242 22 613 1567 2152 Fragments arranged by size: 2152 1567 613 291 242 41 22 ApaI G_GGCC'C Cuts at: 1017 1017 Size: 4928 ApaLI G'TGCA_C Cuts at: 3698 3698 Size: 4928 ApoI r'AATT_y Cuts at: 1932 4281 4537 1932 Size: 2349 256 2323 Fragments arranged by size: 2349 2323 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4928 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4928 AvaI C'yCGr_G Cuts at: 1447 1819 1948 1447 Size: 372 129 4427 Fragments arranged by size: 4427 372 129 BamHI G'GATC_C Cuts at: 1953 1953 Size: 4928 BanI G'GyrC_C Cuts at: 1202 1944 2358 2606 2641 1202 Size: 742 414 248 35 3489 Fragments arranged by size: 3489 742 414 248 35 BanII G_rGCy'C Cuts at: 295 805 944 961 1017 1448 1538 1942 2972 Size: 510 139 17 56 431 90 404 1030 Cuts at: 2972 295 Size: 2251 Fragments arranged by size: 2251 1030 510 431 404 139 90 56 17 BbeI G_GCGC'C Cuts at: 2362 2610 2362 Size: 248 4680 BbsI GAAGACnn'nnnn_ Cuts at: 1403 1403 Size: 4928 BciVI GTATCCnnnnn_n' Cuts at: 2826 3587 4928 2826 Size: 761 1341 2826 Fragments arranged by size: 2826 1341 761 BclI T'GATC_A Cuts at: 513 2448 513 Size: 1935 2993 BglI GCCn_nnn'nGGC Cuts at: 565 928 565 Size: 363 4565 BglII A'GATC_T Cuts at: 224 2443 4480 224 Size: 2219 2037 672 Fragments arranged by size: 2219 2037 672 BlpI GC'TnA_GC Cuts at: 1346 1579 2040 1346 Size: 233 461 4234 Fragments arranged by size: 4234 461 233 Bme1580I G_kGCm'C Cuts at: 1017 1127 1649 2553 2646 3702 1017 Size: 110 522 904 93 1056 2243 Fragments arranged by size: 2243 1056 904 522 110 93 BmgBI CAC'GTC Cuts at: 117 183 117 Size: 66 4862 BmrI ACTGGGnnnn_n' Cuts at: 180 518 1608 2140 2221 2556 4700 180 Size: 338 1090 532 81 335 2144 408 Fragments arranged by size: 2144 1090 532 408 338 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3295 3295 Size: 4928 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 229 830 958 1031 1402 1609 1846 229 Size: 601 128 73 371 207 237 3311 Fragments arranged by size: 3311 601 371 237 207 128 73 Bpu10I CC'TnA_GC Cuts at: 1501 1501 Size: 4928 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 490 2104 2983 3475 3773 490 Size: 1614 879 492 298 1645 Fragments arranged by size: 1645 1614 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 1686 4632 1686 Size: 2946 1982 BsaAI yAC'GTr Cuts at: 656 1296 2281 656 Size: 640 985 3303 Fragments arranged by size: 3303 985 640 BsaBI GATnn'nnATC Cuts at: 6 2442 4479 6 Size: 2436 2037 455 Fragments arranged by size: 2436 2037 455 BsaHI Gr'CG_yC Cuts at: 520 2359 2607 3309 4805 520 Size: 1839 248 702 1496 643 Fragments arranged by size: 1839 1496 702 643 248 BsaWI w'CCGG_w Cuts at: 1319 2117 2638 3316 3590 3737 4148 1319 Size: 798 521 678 274 147 411 2099 Fragments arranged by size: 2099 798 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1267 1297 1267 Size: 30 4898 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 226 784 907 1153 1347 1384 1447 1525 226 Size: 558 123 246 194 37 63 78 3629 Fragments arranged by size: 3629 558 246 194 123 78 63 37 BseYI C'CCAG_C Cuts at: 191 195 810 1694 1811 1916 2353 3111 3688 Size: 4 615 884 117 105 437 758 577 Cuts at: 3688 4184 191 Size: 496 935 Fragments arranged by size: 935 884 758 615 577 496 437 117 105 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 418 816 1633 418 Size: 398 817 3713 Fragments arranged by size: 3713 817 398 BsiEI CG_ry'CG Cuts at: 104 2470 2516 3724 104 Size: 2366 46 1208 1308 Fragments arranged by size: 2366 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 1538 1942 2720 2910 3702 1538 Size: 404 778 190 792 2764 Fragments arranged by size: 2764 792 778 404 190 Bsp1286I G_dGCh'C Cuts at: 295 805 944 961 1017 1127 1448 1538 1649 Size: 510 139 17 56 110 321 90 111 Cuts at: 1649 1942 2553 2646 2720 2910 2972 3702 295 Size: 293 611 93 74 190 62 730 1521 Fragments arranged by size: 1521 730 611 510 321 293 190 139 111 110 93 90 74 62 56 17 BspEI T'CCGG_A Cuts at: 1319 4148 1319 Size: 2829 2099 BspHI T'CATG_A Cuts at: 4104 4104 Size: 4928 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1978 2192 2494 2875 1978 Size: 214 302 381 4031 Fragments arranged by size: 4031 381 302 214 BsrBI CCG'CTC Cuts at: 50 934 1005 3220 3274 4758 4923 50 Size: 884 71 2215 54 1484 165 55 Fragments arranged by size: 2215 1484 884 165 71 55 54 BsrDI GCAATG_nn' Cuts at: 2840 2840 Size: 4928 BsrFI r'CCGG_y Cuts at: 1740 2926 3107 3316 1740 Size: 1186 181 209 3352 Fragments arranged by size: 3352 1186 209 181 BsrGI T'GTAC_A Cuts at: 602 602 Size: 4928 BssHII G'CGCG_C Cuts at: 124 124 Size: 4928 BssSI C'ACGA_G Cuts at: 3199 3557 3199 Size: 358 4570 BstAPI GCAn_nnn'nTGC Cuts at: 1225 1356 1620 1644 2305 1225 Size: 131 264 24 661 3848 Fragments arranged by size: 3848 661 264 131 24 BstBI TT'CG_AA Cuts at: 1521 3289 1521 Size: 1768 3160 BstXI CCAn_nnnn'nTGG Cuts at: 561 1055 1666 561 Size: 494 611 3823 Fragments arranged by size: 3823 611 494 BstYI r'GATC_y Cuts at: 1 224 501 1953 2420 2443 2778 3024 4036 Size: 223 277 1452 467 23 335 246 1012 Cuts at: 4036 4122 4134 4480 1 Size: 86 12 346 449 Fragments arranged by size: 1452 1012 467 449 346 335 277 246 223 86 23 12 Bsu36I CC'TnA_GG Cuts at: 911 1430 911 Size: 519 4409 BtgI C'CryG_G Cuts at: 107 554 710 1835 1910 107 Size: 447 156 1125 75 3125 Fragments arranged by size: 3125 1125 447 156 75 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1527 2878 3059 3222 1527 Size: 1351 181 163 3233 Fragments arranged by size: 3233 1351 181 163 BtsI GCAGTG_nn' Cuts at: 1357 1493 2171 1357 Size: 136 678 4114 Fragments arranged by size: 4114 678 136 DraI TTT'AAA Cuts at: 1927 1927 Size: 4928 DrdI GACnn_nn'nnGTC Cuts at: 1276 1678 2634 3492 1276 Size: 402 956 858 2712 Fragments arranged by size: 2712 956 858 402 EaeI y'GGCC_r Cuts at: 626 1734 1784 1832 2513 2687 3078 3105 4450 Size: 1108 50 48 681 174 391 27 1345 Cuts at: 4450 626 Size: 1104 Fragments arranged by size: 1345 1108 1104 681 391 174 50 48 27 EagI C'GGCC_G Cuts at: 2513 2513 Size: 4928 EarI CTCTTCn'nnn_ Cuts at: 396 1543 2951 3161 396 Size: 1147 1408 210 2163 Fragments arranged by size: 2163 1408 1147 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 3136 3446 3592 4738 3136 Size: 310 146 1146 3326 Fragments arranged by size: 3326 1146 310 146 EcoICRI GAG'CTC Cuts at: 1536 1940 1536 Size: 404 4524 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 229 830 958 1031 1402 1609 1846 2753 3185 Size: 601 128 73 371 207 237 907 432 Cuts at: 3185 3932 4200 229 Size: 747 268 957 Fragments arranged by size: 957 907 747 601 432 371 268 237 207 128 73 EcoO109I rG'GnC_Cy Cuts at: 348 435 531 621 677 743 838 1013 1014 Size: 87 96 90 56 66 95 175 1 Cuts at: 1014 1278 2067 348 Size: 264 789 3209 Fragments arranged by size: 3209 789 264 175 96 95 90 87 66 56 1 EcoRI G'AATT_C Cuts at: 1932 1932 Size: 4928 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2947 2947 Size: 4928 FspI TGC'GCA Cuts at: 2709 2709 Size: 4928 HaeII r_GCGC'y Cuts at: 1175 1887 2362 2610 3632 4611 1175 Size: 712 475 248 1022 979 1492 Fragments arranged by size: 1492 1022 979 712 475 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 346 378 448 480 688 720 1012 1044 1831 Size: 32 70 32 208 32 292 32 787 Cuts at: 1831 1863 2770 2800 2802 2832 346 Size: 32 907 30 2 30 2442 Fragments arranged by size: 2442 907 787 292 208 70 32 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 1967 1967 Size: 4928 KasI G'GCGC_C Cuts at: 2358 2606 2358 Size: 248 4680 KpnI G_GTAC'C Cuts at: 1206 1948 1206 Size: 742 4186 MluI A'CGCG_T Cuts at: 4361 4361 Size: 4928 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 668 1289 3599 3783 668 Size: 621 2310 184 1813 Fragments arranged by size: 2310 1813 621 184 MscI TGG'CCA Cuts at: 628 1736 1786 2689 628 Size: 1108 50 903 2867 Fragments arranged by size: 2867 1108 903 50 MslI CAynn'nnrTG Cuts at: 1664 2917 3044 1664 Size: 1253 127 3548 Fragments arranged by size: 3548 1253 127 MspA1I CmG'CkG Cuts at: 195 721 1474 1677 1782 2039 2353 2713 3726 Size: 526 753 203 105 257 314 360 1013 Cuts at: 3726 3971 195 Size: 245 1152 Fragments arranged by size: 1152 1013 753 526 360 314 257 245 203 105 NaeI GCC'GGC Cuts at: 1742 3109 1742 Size: 1367 3561 NarI GG'CG_CC Cuts at: 2359 2607 2359 Size: 248 4680 NcoI C'CATG_G Cuts at: 107 554 710 1910 107 Size: 447 156 1200 3125 Fragments arranged by size: 3125 1200 447 156 NgoMIV G'CCGG_C Cuts at: 1740 3107 1740 Size: 1367 3561 NspI r_CATG'y Cuts at: 705 996 1645 1981 2481 3388 705 Size: 291 649 336 500 907 2245 Fragments arranged by size: 2245 907 649 500 336 291 PasI CC'CwG_GG Cuts at: 864 1463 864 Size: 599 4329 PciI A'CATG_T Cuts at: 3384 3384 Size: 4928 PflMI CCAn_nnn'nTGG Cuts at: 170 170 Size: 4928 PfoI T'CCnGG_A Cuts at: 487 952 1565 1625 487 Size: 465 613 60 3790 Fragments arranged by size: 3790 613 465 60 PmeI GTTT'AAAC Cuts at: 1927 1927 Size: 4928 PmlI CAC'GTG Cuts at: 656 1296 656 Size: 640 4288 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 442 474 4067 4099 442 Size: 32 3593 32 1271 Fragments arranged by size: 3593 1271 32 32 PpuMI rG'GwC_Cy Cuts at: 435 531 621 743 1278 435 Size: 96 90 122 535 4085 Fragments arranged by size: 4085 535 122 96 90 PspOMI G'GGCC_C Cuts at: 1013 1013 Size: 4928 PstI C_TGCA'G Cuts at: 1187 1331 1368 1781 1975 2660 1187 Size: 144 37 413 194 685 3455 Fragments arranged by size: 3455 685 413 194 144 37 PvuI CG_AT'CG Cuts at: 104 104 Size: 4928 PvuII CAG'CTG Cuts at: 195 1677 1782 2353 2713 195 Size: 1482 105 571 360 2410 Fragments arranged by size: 2410 1482 571 360 105 RsrII CG'GwC_CG Cuts at: 3123 3123 Size: 4928 SacI G_AGCT'C Cuts at: 1538 1942 1538 Size: 404 4524 SalI G'TCGA_C Cuts at: 1965 1965 Size: 4928 SanDI GG'GwC_CC Cuts at: 435 743 435 Size: 308 4620 SapI GCTCTTCn'nnn_ Cuts at: 1543 2951 3161 1543 Size: 1408 210 3310 Fragments arranged by size: 3310 1408 210 SbfI CC_TGCA'GG Cuts at: 1975 1975 Size: 4928 ScaI AGT'ACT Cuts at: 4445 4445 Size: 4928 SfcI C'TryA_G Cuts at: 33 306 1183 1327 1364 1777 1971 2656 3649 Size: 273 877 144 37 413 194 685 993 Cuts at: 3649 3840 33 Size: 191 1121 Fragments arranged by size: 1121 993 877 685 413 273 194 191 144 37 SfiI GGCCn_nnn'nGGCC Cuts at: 565 928 565 Size: 363 4565 SfoI GGC'GCC Cuts at: 2360 2608 2360 Size: 248 4680 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4928 SmaI CCC'GGG Cuts at: 1821 1950 1821 Size: 129 4799 SmlI C'TyrA_G Cuts at: 505 2083 2998 3490 3752 505 Size: 1578 915 492 262 1681 Fragments arranged by size: 1681 1578 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4928 SphI G_CATG'C Cuts at: 1981 2481 1981 Size: 500 4428 SrfI GCCC'GGGC Cuts at: 1821 1821 Size: 4928 StuI AGG'CCT Cuts at: 916 1113 916 Size: 197 4731 StyI C'CwwG_G Cuts at: 107 554 710 926 944 1082 1910 2062 2416 Size: 447 156 216 18 138 828 152 354 Cuts at: 2416 107 Size: 2619 Fragments arranged by size: 2619 828 447 354 216 156 152 138 18 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 856 2511 2644 3103 3312 856 Size: 1655 133 459 209 2472 Fragments arranged by size: 2472 1655 459 209 133 TatI w'GTAC_w Cuts at: 602 1283 1436 4443 602 Size: 681 153 3007 1087 Fragments arranged by size: 3007 1087 681 153 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 645 677 4445 4477 645 Size: 32 3768 32 1096 Fragments arranged by size: 3768 1096 32 32 Tth111I GACn'n_nGTC Cuts at: 2725 2725 Size: 4928 XbaI T'CTAG_A Cuts at: 1422 1959 1422 Size: 537 4391 XcmI CCAnnnn_n'nnnnTGG Cuts at: 114 561 933 2423 114 Size: 447 372 1490 2619 Fragments arranged by size: 2619 1490 447 372 XmaI C'CCGG_G Cuts at: 1819 1948 1819 Size: 129 4799 XmnI GAAnn'nnTTC Cuts at: 457 640 457 Size: 183 4745 Enzymes that do cut: AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI SrfI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AflII AleI AloI AscI AvrII BaeI BbvCI BcgI BfrBI BmtI BsiWI BsmI BsmBI BstEII BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PshAI PsiI PspXI PsrI SacII SexAI SgrAI SnaBI SspI SwaI XhoI ZraI