Restriction map for
pF1KE0346
(Circular) (Six-base) MAPSORT of: pF1KE0346.seq Check: 8745 from: 1 to: 5018
pF1KE0346 5018 bp
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 579 579
Size: 5018
AatII G_ACGT'C
Cuts at: 202 866 202
Size: 664 4354
AccI GT'mk_AC
Cuts at: 2056 2056
Size: 5018
Acc65I G'GTAC_C
Cuts at: 2034 2034
Size: 5018
AclI AA'CG_TT
Cuts at: 4858 4858
Size: 5018
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 1501 2843 3275 4022 4290 190
Size: 1311 1342 432 747 268 918
Fragments arranged by size:
1342 1311 918 747 432 268
AfeI AGC'GCT
Cuts at: 777 1019 777
Size: 242 4776
AflIII A'CryG_T
Cuts at: 390 2370 3474 4451 390
Size: 1980 1104 977 957
Fragments arranged by size:
1980 1104 977 957
AgeI A'CCGG_T
Cuts at: 3406 3406
Size: 5018
AhdI GACnn_n'nnGTC
Cuts at: 1269 1269
Size: 5018
AleI CACnn'nnGTG
Cuts at: 1318 1318
Size: 5018
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 165 384 165
Size: 219 4799
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 523 555 523
Size: 32 4986
AlwNI CAG_nnn'CTG
Cuts at: 159 1287 2323 3890 159
Size: 1128 1036 1567 1287
Fragments arranged by size:
1567 1287 1128 1036
ApaLI G'TGCA_C
Cuts at: 1186 3788 1186
Size: 2602 2416
ApoI r'AATT_y
Cuts at: 379 2022 4371 4627 379
Size: 1643 2349 256 770
Fragments arranged by size:
2349 1643 770 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5018
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5018
AvaI C'yCGr_G
Cuts at: 113 264 2038 113
Size: 151 1774 3093
Fragments arranged by size:
3093 1774 151
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 413 446 413
Size: 33 4985
BamHI G'GATC_C
Cuts at: 2043 2043
Size: 5018
BanI G'GyrC_C
Cuts at: 286 496 591 949 987 1203 1216 1983 2034
Size: 210 95 358 38 216 13 767 51
Cuts at: 2034 2448 2696 2731 286
Size: 414 248 35 2573
Fragments arranged by size:
2573 767 414 358 248 216 210 95
51 38 35 13
BanII G_rGCy'C
Cuts at: 265 409 672 1163 1235 1417 1698 2032 3062
Size: 144 263 491 72 182 281 334 1030
Cuts at: 3062 265
Size: 2221
Fragments arranged by size:
2221 1030 491 334 281 263 182 144
72
BbeI G_GCGC'C
Cuts at: 500 2452 2700 500
Size: 1952 248 2818
Fragments arranged by size:
2818 1952 248
BbsI GAAGACnn'nnnn_
Cuts at: 1349 1724 1349
Size: 375 4643
BbvCI CC'TCA_GC
Cuts at: 1569 1569
Size: 5018
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 850 884 1693 1727 850
Size: 34 809 34 4141
Fragments arranged by size:
4141 809 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 441 2916 3677 5018 441
Size: 2475 761 1341 441
Fragments arranged by size:
2475 1341 761 441
BclI T'GATC_A
Cuts at: 2538 2538
Size: 5018
BglI GCCn_nnn'nGGC
Cuts at: 346 505 1249 346
Size: 159 744 4115
Fragments arranged by size:
4115 744 159
BglII A'GATC_T
Cuts at: 2533 4570 2533
Size: 2037 2981
BlpI GC'TnA_GC
Cuts at: 1108 1675 2130 1108
Size: 567 455 3996
Fragments arranged by size:
3996 567 455
Bme1580I G_kGCm'C
Cuts at: 390 596 992 1190 1219 1447 2643 2736 3792
Size: 206 396 198 29 228 1196 93 1056
Cuts at: 3792 390
Size: 1616
Fragments arranged by size:
1616 1196 1056 396 228 206 198 93
29
BmrI ACTGGGnnnn_n'
Cuts at: 1638 2230 2311 2646 4790 1638
Size: 592 81 335 2144 1866
Fragments arranged by size:
2144 1866 592 335 81
BmtI G_CTAG'C
Cuts at: 137 137
Size: 5018
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3385 3385
Size: 5018
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 199 537 792 861 1288 1550 1966 199
Size: 338 255 69 427 262 416 3251
Fragments arranged by size:
3251 427 416 338 262 255 69
Bpu10I CC'TnA_GC
Cuts at: 1466 1569 1759 1466
Size: 103 190 4725
Fragments arranged by size:
4725 190 103
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2194 3073 3565 3863 2194
Size: 879 492 298 3349
Fragments arranged by size:
3349 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 274 562 4722 274
Size: 288 4160 570
Fragments arranged by size:
4160 570 288
BsaAI yAC'GTr
Cuts at: 276 391 429 576 1923 2371 276
Size: 115 38 147 1347 448 2923
Fragments arranged by size:
2923 1347 448 147 115 38
BsaBI GATnn'nnATC
Cuts at: 6 2532 4569 6
Size: 2526 2037 455
Fragments arranged by size:
2526 2037 455
BsaHI Gr'CG_yC
Cuts at: 199 497 815 863 2449 2697 3399 4895 199
Size: 298 318 48 1586 248 702 1496 322
Fragments arranged by size:
1586 1496 702 322 318 298 248 48
BsaWI w'CCGG_w
Cuts at: 2207 2728 3406 3680 3827 4238 2207
Size: 521 678 274 147 411 2987
Fragments arranged by size:
2987 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 659 689 1523 1553 659
Size: 30 834 30 4124
Fragments arranged by size:
4124 834 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 466 898 1105 1225 1963 466
Size: 432 207 120 738 3521
Fragments arranged by size:
3521 738 432 207 120
BseYI C'CCAG_C
Cuts at: 758 1366 1380 1975 2443 3201 3778 4274 758
Size: 608 14 595 468 758 577 496 1502
Fragments arranged by size:
1502 758 608 595 577 496 468 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1600 1978 1600
Size: 378 4640
BsiEI CG_ry'CG
Cuts at: 104 2560 2606 3814 104
Size: 2456 46 1208 1308
Fragments arranged by size:
2456 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 281 323 1190 1417 2032 2810 3000 3792 281
Size: 42 867 227 615 778 190 792 1507
Fragments arranged by size:
1507 867 792 778 615 227 190 42
BsmBI CGTCTCn'nnnn_
Cuts at: 190 190
Size: 5018
Bsp1286I G_dGCh'C
Cuts at: 265 281 323 390 409 596 672 992 1163
Size: 16 42 67 19 187 76 320 171
Cuts at: 1163 1190 1219 1235 1417 1447 1698 2032 2643
Size: 27 29 16 182 30 251 334 611
Cuts at: 2643 2736 2810 3000 3062 3792 265
Size: 93 74 190 62 730 1491
Fragments arranged by size:
1491 730 611 334 320 251 190 187
182 171 93 76 74 67 62 42
30 29 27 19 16 16
BspEI T'CCGG_A
Cuts at: 4238 4238
Size: 5018
BspHI T'CATG_A
Cuts at: 4194 4194
Size: 5018
BspMI ACCTGCnnnn'nnnn_
Cuts at: 579 2068 2282 2584 2965 579
Size: 1489 214 302 381 2632
Fragments arranged by size:
2632 1489 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3310 3364 4848 5013 50
Size: 3260 54 1484 165 55
Fragments arranged by size:
3260 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 128 866 938 2930 128
Size: 738 72 1992 2216
Fragments arranged by size:
2216 1992 738 72
BsrFI r'CCGG_y
Cuts at: 823 3016 3197 3406 823
Size: 2193 181 209 2435
Fragments arranged by size:
2435 2193 209 181
BsrGI T'GTAC_A
Cuts at: 1449 1449
Size: 5018
BssSI C'ACGA_G
Cuts at: 3289 3647 3289
Size: 358 4660
BstAPI GCAn_nnn'nTGC
Cuts at: 949 1965 2395 949
Size: 1016 430 3572
Fragments arranged by size:
3572 1016 430
BstBI TT'CG_AA
Cuts at: 3379 3379
Size: 5018
BstXI CCAn_nnnn'nTGG
Cuts at: 303 303
Size: 5018
BstYI r'GATC_y
Cuts at: 1 1730 2043 2510 2533 2868 3114 4126 4212
Size: 1729 313 467 23 335 246 1012 86
Cuts at: 4212 4224 4570 1
Size: 12 346 449
Fragments arranged by size:
1729 1012 467 449 346 335 313 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1492 1492
Size: 5018
BtgI C'CryG_G
Cuts at: 107 290 434 503 898 1145 1736 107
Size: 183 144 69 395 247 591 3389
Fragments arranged by size:
3389 591 395 247 183 144 69
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 906 2968 3149 3312 906
Size: 2062 181 163 2612
Fragments arranged by size:
2612 2062 181 163
BtsI GCAGTG_nn'
Cuts at: 202 827 1252 1541 1853 2261 202
Size: 625 425 289 312 408 2959
Fragments arranged by size:
2959 625 425 408 312 289
DraI TTT'AAA
Cuts at: 2017 2017
Size: 5018
DraIII CAC_nnn'GTG
Cuts at: 1923 1923
Size: 5018
DrdI GACnn_nn'nnGTC
Cuts at: 2724 3582 2724
Size: 858 4160
EaeI y'GGCC_r
Cuts at: 293 338 704 821 917 1148 1322 2001 2603
Size: 45 366 117 96 231 174 679 602
Cuts at: 2603 2777 3168 3195 4540 293
Size: 174 391 27 1345 771
Fragments arranged by size:
1345 771 679 602 391 366 231 174
174 117 96 45 27
EagI C'GGCC_G
Cuts at: 2603 2603
Size: 5018
EarI CTCTTCn'nnn_
Cuts at: 242 733 1123 1168 3041 3251 242
Size: 491 390 45 1873 210 2009
Fragments arranged by size:
2009 1873 491 390 210 45
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3226 3536 3682 4828 3226
Size: 310 146 1146 3416
Fragments arranged by size:
3416 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1415 2030 1415
Size: 615 4403
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 199 537 792 861 1288 1501 1550 1966
Size: 9 338 255 69 427 213 49 416
Cuts at: 1966 2843 3275 4022 4290 190
Size: 877 432 747 268 918
Fragments arranged by size:
918 877 747 432 427 416 338 268
255 213 69 49 9
EcoNI CCTnn'n_nnAGG
Cuts at: 1469 1469
Size: 5018
EcoO109I rG'GnC_Cy
Cuts at: 1496 1995 2157 1496
Size: 499 162 4357
Fragments arranged by size:
4357 499 162
EcoRI G'AATT_C
Cuts at: 2022 2022
Size: 5018
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3037 3037
Size: 5018
FspI TGC'GCA
Cuts at: 2799 2799
Size: 5018
HaeII r_GCGC'y
Cuts at: 213 500 779 1021 1552 2452 2700 3722 4701
Size: 287 279 242 531 900 248 1022 979
Cuts at: 4701 213
Size: 530
Fragments arranged by size:
1022 979 900 531 530 287 279 248
242
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 997 1029 1255 1287 2860 2890 2892 2922 997
Size: 32 226 32 1573 30 2 30 3093
Fragments arranged by size:
3093 1573 226 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2057 2057
Size: 5018
KasI G'GCGC_C
Cuts at: 496 2448 2696 496
Size: 1952 248 2818
Fragments arranged by size:
2818 1952 248
KpnI G_GTAC'C
Cuts at: 2038 2038
Size: 5018
MluI A'CGCG_T
Cuts at: 4451 4451
Size: 5018
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3689 3873 3689
Size: 184 4834
MscI TGG'CCA
Cuts at: 295 919 1150 1324 2003 2779 295
Size: 624 231 174 679 776 2534
Fragments arranged by size:
2534 776 679 624 231 174
MslI CAynn'nnrTG
Cuts at: 147 1318 3007 3134 147
Size: 1171 1689 127 2031
Fragments arranged by size:
2031 1689 1171 127
MspA1I CmG'CkG
Cuts at: 177 343 946 1041 1075 1437 1890 2129 2443
Size: 166 603 95 34 362 453 239 314
Cuts at: 2443 2803 3816 4061 177
Size: 360 1013 245 1134
Fragments arranged by size:
1134 1013 603 453 362 360 314 245
239 166 95 34
NaeI GCC'GGC
Cuts at: 825 3199 825
Size: 2374 2644
NarI GG'CG_CC
Cuts at: 497 2449 2697 497
Size: 1952 248 2818
Fragments arranged by size:
2818 1952 248
NcoI C'CATG_G
Cuts at: 107 434 1145 1736 107
Size: 327 711 591 3389
Fragments arranged by size:
3389 711 591 327
NdeI CA'TA_TG
Cuts at: 2006 2006
Size: 5018
NgoMIV G'CCGG_C
Cuts at: 823 3197 823
Size: 2374 2644
NheI G'CTAG_C
Cuts at: 133 133
Size: 5018
NspI r_CATG'y
Cuts at: 2071 2571 3478 2071
Size: 500 907 3611
Fragments arranged by size:
3611 907 500
PasI CC'CwG_GG
Cuts at: 1934 1934
Size: 5018
PciI A'CATG_T
Cuts at: 3474 3474
Size: 5018
PmeI GTTT'AAAC
Cuts at: 2017 2017
Size: 5018
PmlI CAC'GTG
Cuts at: 391 576 391
Size: 185 4833
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4157 4189 4157
Size: 32 4986
PpuMI rG'GwC_Cy
Cuts at: 1995 1995
Size: 5018
PstI C_TGCA'G
Cuts at: 2065 2750 2065
Size: 685 4333
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5018
PvuII CAG'CTG
Cuts at: 946 1075 1437 2443 2803 946
Size: 129 362 1006 360 3161
Fragments arranged by size:
3161 1006 362 360 129
RsrII CG'GwC_CG
Cuts at: 3213 3213
Size: 5018
SacI G_AGCT'C
Cuts at: 1417 2032 1417
Size: 615 4403
SalI G'TCGA_C
Cuts at: 2055 2055
Size: 5018
SapI GCTCTTCn'nnn_
Cuts at: 1168 3041 3251 1168
Size: 1873 210 2935
Fragments arranged by size:
2935 1873 210
SbfI CC_TGCA'GG
Cuts at: 2065 2065
Size: 5018
ScaI AGT'ACT
Cuts at: 4535 4535
Size: 5018
SfcI C'TryA_G
Cuts at: 33 1047 1432 2061 2746 3739 3930 33
Size: 1014 385 629 685 993 191 1121
Fragments arranged by size:
1121 1014 993 685 629 385 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 346 346
Size: 5018
SfoI GGC'GCC
Cuts at: 498 2450 2698 498
Size: 1952 248 2818
Fragments arranged by size:
2818 1952 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5018
SmaI CCC'GGG
Cuts at: 115 2040 115
Size: 1925 3093
SmlI C'TyrA_G
Cuts at: 2173 3088 3580 3842 2173
Size: 915 492 262 3349
Fragments arranged by size:
3349 915 492 262
SnaBI TAC'GTA
Cuts at: 429 429
Size: 5018
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5018
SphI G_CATG'C
Cuts at: 2071 2571 2071
Size: 500 4518
StyI C'CwwG_G
Cuts at: 107 296 434 1145 1293 1736 2152 2506 107
Size: 189 138 711 148 443 416 354 2619
Fragments arranged by size:
2619 711 443 416 354 189 148 138
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 238 1800 2601 2734 3193 3402 238
Size: 1562 801 133 459 209 1854
Fragments arranged by size:
1854 1562 801 459 209 133
TatI w'GTAC_w
Cuts at: 1449 4533 1449
Size: 3084 1934
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 659 691 1523 1555 1832 1864 4535 4567 659
Size: 32 832 32 277 32 2671 32 1110
Fragments arranged by size:
2671 1110 832 277 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2815 2815
Size: 5018
XbaI T'CTAG_A
Cuts at: 2049 2049
Size: 5018
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 135 1920 1941 2513 135
Size: 1785 21 572 2640
Fragments arranged by size:
2640 1785 572 21
XmaI C'CCGG_G
Cuts at: 113 2038 113
Size: 1925 3093
ZraI GAC'GTC
Cuts at: 200 864 200
Size: 664 4354
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NspI PasI PciI PmeI PmlI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AflII ApaI AscI AvrII BfrBI BmgBI BsiWI BsmI
BssHII BstEII BstZ17I ClaI CspCI EcoRV FseI FspAI
HindIII HpaI MfeI NotI NruI NsiI PacI PflMI
PfoI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SgrAI SrfI SspI StuI SwaI XhoI XmnI