Restriction map for
pF1KE0348
(Circular) (Six-base) MAPSORT of: pF1KE0348.seq Check: 1377 from: 1 to: 4622
pF1KE0348 4622 bp
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 354 946 954 1079 354
Size: 592 8 125 3897
Fragments arranged by size:
3897 592 125 8
AccI GT'mk_AC
Cuts at: 1019 1502 1660 1019
Size: 483 158 3981
Fragments arranged by size:
3981 483 158
Acc65I G'GTAC_C
Cuts at: 1638 1638
Size: 4622
AclI AA'CG_TT
Cuts at: 4462 4462
Size: 4622
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 430 664 2447 2879 3626 3894 430
Size: 234 1783 432 747 268 1158
Fragments arranged by size:
1783 1158 747 432 268 234
AfeI AGC'GCT
Cuts at: 994 994
Size: 4622
AflIII A'CryG_T
Cuts at: 1974 3078 4055 1974
Size: 1104 977 2541
Fragments arranged by size:
2541 1104 977
AgeI A'CCGG_T
Cuts at: 3010 3010
Size: 4622
AlwNI CAG_nnn'CTG
Cuts at: 387 408 576 651 720 891 1927 3494 387
Size: 21 168 75 69 171 1036 1567 1515
Fragments arranged by size:
1567 1515 1036 171 168 75 69 21
ApaI G_GGCC'C
Cuts at: 1567 1567
Size: 4622
ApaLI G'TGCA_C
Cuts at: 1207 3392 1207
Size: 2185 2437
ApoI r'AATT_y
Cuts at: 1128 1626 3975 4231 1128
Size: 498 2349 256 1519
Fragments arranged by size:
2349 1519 498 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4622
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4622
AvaI C'yCGr_G
Cuts at: 700 1642 700
Size: 942 3680
BamHI G'GATC_C
Cuts at: 1647 1647
Size: 4622
BanI G'GyrC_C
Cuts at: 511 905 1120 1361 1638 2052 2300 2335 511
Size: 394 215 241 277 414 248 35 2798
Fragments arranged by size:
2798 414 394 277 248 241 215 35
BanII G_rGCy'C
Cuts at: 269 1567 1636 2666 269
Size: 1298 69 1030 2225
Fragments arranged by size:
2225 1298 1030 69
BbeI G_GCGC'C
Cuts at: 1365 2056 2304 1365
Size: 691 248 3683
Fragments arranged by size:
3683 691 248
BbvCI CC'TCA_GC
Cuts at: 757 757
Size: 4622
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1261 1295 90
Size: 34 1137 34 3417
Fragments arranged by size:
3417 1137 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2520 3281 4622 2520
Size: 761 1341 2520
Fragments arranged by size:
2520 1341 761
BclI T'GATC_A
Cuts at: 2142 2142
Size: 4622
BglII A'GATC_T
Cuts at: 2137 4174 2137
Size: 2037 2585
BlpI GC'TnA_GC
Cuts at: 569 787 1353 1734 569
Size: 218 566 381 3457
Fragments arranged by size:
3457 566 381 218
Bme1580I G_kGCm'C
Cuts at: 514 1211 1443 1567 2247 2340 3396 514
Size: 697 232 124 680 93 1056 1740
Fragments arranged by size:
1740 1056 697 680 232 124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1269 1278 1834 1915 2250 4394 1269
Size: 9 556 81 335 2144 1497
Fragments arranged by size:
2144 1497 556 335 81 9
BmtI G_CTAG'C
Cuts at: 124 124
Size: 4622
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2989 2989
Size: 4622
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 276 295 397 475 655 658 742 834 1475
Size: 19 102 78 180 3 84 92 641
Cuts at: 1475 276
Size: 3423
Fragments arranged by size:
3423 641 180 102 92 84 78 19
3
Bpu10I CC'TnA_GC
Cuts at: 757 757
Size: 4622
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 462 1798 2677 3169 3467 462
Size: 1336 879 492 298 1617
Fragments arranged by size:
1617 1336 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 294 607 718 1163 4326 294
Size: 313 111 445 3163 590
Fragments arranged by size:
3163 590 445 313 111
BsaAI yAC'GTr
Cuts at: 1975 1975
Size: 4622
BsaBI GATnn'nnATC
Cuts at: 6 2136 4173 6
Size: 2130 2037 455
Fragments arranged by size:
2130 2037 455
BsaHI Gr'CG_yC
Cuts at: 1362 1472 1499 2053 2301 3003 4499 1362
Size: 110 27 554 248 702 1496 1485
Fragments arranged by size:
1496 1485 702 554 248 110 27
BsaWI w'CCGG_w
Cuts at: 1811 2332 3010 3284 3431 3842 1811
Size: 521 678 274 147 411 2591
Fragments arranged by size:
2591 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 133 754 133
Size: 621 4001
BseYI C'CCAG_C
Cuts at: 116 359 507 1173 1177 1191 1567 2047 2805
Size: 243 148 666 4 14 376 480 758
Cuts at: 2805 3382 3878 116
Size: 577 496 860
Fragments arranged by size:
860 758 666 577 496 480 376 243
148 14 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 322 1001 322
Size: 679 3943
BsiEI CG_ry'CG
Cuts at: 104 2164 2210 3418 104
Size: 2060 46 1208 1308
Fragments arranged by size:
2060 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1187 1211 1636 2414 2604 3396 1187
Size: 24 425 778 190 792 2413
Fragments arranged by size:
2413 792 778 425 190 24
Bsp1286I G_dGCh'C
Cuts at: 269 514 1187 1211 1443 1567 1636 2247 2340
Size: 245 673 24 232 124 69 611 93
Cuts at: 2340 2414 2604 2666 3396 269
Size: 74 190 62 730 1495
Fragments arranged by size:
1495 730 673 611 245 232 190 124
93 74 69 62 24
BspEI T'CCGG_A
Cuts at: 3842 3842
Size: 4622
BspHI T'CATG_A
Cuts at: 3798 3798
Size: 4622
BspMI ACCTGCnnnn'nnnn_
Cuts at: 354 450 946 954 1079 1672 1886 2188 2569
Size: 96 496 8 125 593 214 302 381
Cuts at: 2569 354
Size: 2407
Fragments arranged by size:
2407 593 496 381 302 214 125 96
8
BsrBI CCG'CTC
Cuts at: 50 2914 2968 4452 4617 50
Size: 2864 54 1484 165 55
Fragments arranged by size:
2864 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1203 2534 1203
Size: 1331 3291
BsrFI r'CCGG_y
Cuts at: 2620 2801 3010 2620
Size: 181 209 4232
Fragments arranged by size:
4232 209 181
BssHII G'CGCG_C
Cuts at: 695 695
Size: 4622
BssSI C'ACGA_G
Cuts at: 1270 2893 3251 1270
Size: 1623 358 2641
Fragments arranged by size:
2641 1623 358
BstAPI GCAn_nnn'nTGC
Cuts at: 387 879 912 1999 387
Size: 492 33 1087 3010
Fragments arranged by size:
3010 1087 492 33
BstBI TT'CG_AA
Cuts at: 2983 2983
Size: 4622
BstXI CCAn_nnnn'nTGG
Cuts at: 168 636 997 168
Size: 468 361 3793
Fragments arranged by size:
3793 468 361
BstYI r'GATC_y
Cuts at: 1 542 986 1647 2114 2137 2472 2718 3730
Size: 541 444 661 467 23 335 246 1012
Cuts at: 3730 3816 3828 4174 1
Size: 86 12 346 449
Fragments arranged by size:
1012 661 541 467 449 444 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 284 284
Size: 4622
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2572 2753 2916 2572
Size: 181 163 4278
Fragments arranged by size:
4278 181 163
BtsI GCAGTG_nn'
Cuts at: 334 385 409 533 1086 1314 1576 1865 334
Size: 51 24 124 553 228 262 289 3091
Fragments arranged by size:
3091 553 289 262 228 124 51 24
DraI TTT'AAA
Cuts at: 1621 1621
Size: 4622
DraIII CAC_nnn'GTG
Cuts at: 404 912 1582 404
Size: 508 670 3444
Fragments arranged by size:
3444 670 508
DrdI GACnn_nn'nnGTC
Cuts at: 621 2328 3186 621
Size: 1707 858 2057
Fragments arranged by size:
2057 1707 858
EaeI y'GGCC_r
Cuts at: 228 326 746 2207 2381 2772 2799 4144 228
Size: 98 420 1461 174 391 27 1345 706
Fragments arranged by size:
1461 1345 706 420 391 174 98 27
EagI C'GGCC_G
Cuts at: 2207 2207
Size: 4622
EarI CTCTTCn'nnn_
Cuts at: 2645 2855 2645
Size: 210 4412
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 208 2830 3140 3286 4432 208
Size: 2622 310 146 1146 398
Fragments arranged by size:
2622 1146 398 310 146
EcoICRI GAG'CTC
Cuts at: 1634 1634
Size: 4622
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 276 295 397 430 475 655 658 664 742
Size: 19 102 33 45 180 3 6 78
Cuts at: 742 834 1475 2447 2879 3626 3894 276
Size: 92 641 972 432 747 268 1004
Fragments arranged by size:
1004 972 747 641 432 268 180 102
92 78 45 33 19 6 3
EcoNI CCTnn'n_nnAGG
Cuts at: 859 859
Size: 4622
EcoO109I rG'GnC_Cy
Cuts at: 280 717 1563 1564 1761 280
Size: 437 846 1 197 3141
Fragments arranged by size:
3141 846 437 197 1
EcoRI G'AATT_C
Cuts at: 1626 1626
Size: 4622
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 456 2641 456
Size: 2185 2437
FspI TGC'GCA
Cuts at: 2403 2403
Size: 4622
HaeII r_GCGC'y
Cuts at: 803 996 1110 1365 2056 2304 3326 4305 803
Size: 193 114 255 691 248 1022 979 1120
Fragments arranged by size:
1120 1022 979 691 255 248 193 114
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2464 2494 2496 2526 2464
Size: 30 2 30 4560
Fragments arranged by size:
4560 30 30 2
HincII GTy'rAC
Cuts at: 1661 1661
Size: 4622
KasI G'GCGC_C
Cuts at: 1361 2052 2300 1361
Size: 691 248 3683
Fragments arranged by size:
3683 691 248
KpnI G_GTAC'C
Cuts at: 1642 1642
Size: 4622
MluI A'CGCG_T
Cuts at: 4055 4055
Size: 4622
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 326 3293 3477 326
Size: 2967 184 1471
Fragments arranged by size:
2967 1471 184
MscI TGG'CCA
Cuts at: 328 748 2383 328
Size: 420 1635 2567
Fragments arranged by size:
2567 1635 420
MslI CAynn'nnrTG
Cuts at: 166 1030 2611 2738 166
Size: 864 1581 127 2050
Fragments arranged by size:
2050 1581 864 127
MspA1I CmG'CkG
Cuts at: 375 648 888 1137 1177 1349 1547 1733 2047
Size: 273 240 249 40 172 198 186 314
Cuts at: 2047 2407 3420 3665 375
Size: 360 1013 245 1332
Fragments arranged by size:
1332 1013 360 314 273 249 245 240
198 186 172 40
NaeI GCC'GGC
Cuts at: 2803 2803
Size: 4622
NarI GG'CG_CC
Cuts at: 1362 2053 2301 1362
Size: 691 248 3683
Fragments arranged by size:
3683 691 248
NgoMIV G'CCGG_C
Cuts at: 2801 2801
Size: 4622
NheI G'CTAG_C
Cuts at: 120 120
Size: 4622
NspI r_CATG'y
Cuts at: 1675 2175 3082 1675
Size: 500 907 3215
Fragments arranged by size:
3215 907 500
PciI A'CATG_T
Cuts at: 3078 3078
Size: 4622
PflMI CCAn_nnn'nTGG
Cuts at: 891 891
Size: 4622
PfoI T'CCnGG_A
Cuts at: 971 971
Size: 4622
PmeI GTTT'AAAC
Cuts at: 1621 1621
Size: 4622
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 952 984 3761 3793 952
Size: 32 2777 32 1781
Fragments arranged by size:
2777 1781 32 32
PshAI GACnn'nnGTC
Cuts at: 1424 1424
Size: 4622
PspOMI G'GGCC_C
Cuts at: 1563 1563
Size: 4622
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1306 1338 1306
Size: 32 4590
PstI C_TGCA'G
Cuts at: 134 392 863 1076 1385 1669 2354 134
Size: 258 471 213 309 284 685 2402
Fragments arranged by size:
2402 685 471 309 284 258 213
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4622
PvuII CAG'CTG
Cuts at: 375 648 888 1177 2047 2407 375
Size: 273 240 289 870 360 2590
Fragments arranged by size:
2590 870 360 289 273 240
RsrII CG'GwC_CG
Cuts at: 2817 2817
Size: 4622
SacI G_AGCT'C
Cuts at: 1636 1636
Size: 4622
SalI G'TCGA_C
Cuts at: 1659 1659
Size: 4622
SapI GCTCTTCn'nnn_
Cuts at: 2645 2855 2645
Size: 210 4412
SbfI CC_TGCA'GG
Cuts at: 863 1669 863
Size: 806 3816
ScaI AGT'ACT
Cuts at: 4139 4139
Size: 4622
SexAI A'CCwGG_T
Cuts at: 908 908
Size: 4622
SfcI C'TryA_G
Cuts at: 33 130 234 388 655 859 1072 1381 1665
Size: 97 104 154 267 204 213 309 284
Cuts at: 1665 2350 3343 3534 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 685 309 284 267 213 204
191 154 104 97
SfoI GGC'GCC
Cuts at: 1363 2054 2302 1363
Size: 691 248 3683
Fragments arranged by size:
3683 691 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4622
SmaI CCC'GGG
Cuts at: 1644 1644
Size: 4622
SmlI C'TyrA_G
Cuts at: 441 700 1777 2692 3184 3446 441
Size: 259 1077 915 492 262 1617
Fragments arranged by size:
1617 1077 915 492 262 259
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4622
SphI G_CATG'C
Cuts at: 1675 2175 1675
Size: 500 4122
StuI AGG'CCT
Cuts at: 1276 1276
Size: 4622
StyI C'CwwG_G
Cuts at: 1756 2110 1756
Size: 354 4268
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 373 571 2205 2338 2797 3006 373
Size: 198 1634 133 459 209 1989
Fragments arranged by size:
1989 1634 459 209 198 133
TatI w'GTAC_w
Cuts at: 1526 4137 1526
Size: 2611 2011
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 203 235 4139 4171 203
Size: 32 3904 32 654
Fragments arranged by size:
3904 654 32 32
Tth111I GACn'n_nGTC
Cuts at: 2419 2419
Size: 4622
XbaI T'CTAG_A
Cuts at: 1653 1653
Size: 4622
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 165 504 633 1450 2117 165
Size: 339 129 817 667 2670
Fragments arranged by size:
2670 817 667 339 129
XhoI C'TCGA_G
Cuts at: 700 700
Size: 4622
XmaI C'CCGG_G
Cuts at: 1642 1642
Size: 4622
XmnI GAAnn'nnTTC
Cuts at: 1060 1060
Size: 4622
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NheI NspI PciI
PflMI PfoI PmeI PpiI PshAI PspOMI PsrI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI AscI AvrII
BaeI BbsI BfrBI BglI BmgBI BsaXI BsiWI BsmI
BsmBI BsrGI BstEII BstZ17I BtgI ClaI CspCI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NotI
NruI NsiI PacI PasI PmlI PpuMI PsiI PspXI
SacII SanDI SfiI SgrAI SnaBI SrfI SspI SwaI
ZraI