Restriction map for
pF1KE0349
(Circular) (Six-base) MAPSORT of: pF1KE0349.seq Check: 5001 from: 1 to: 5267
pF1KE0349 5267 bp
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AccI GT'mk_AC
Cuts at: 2305 2305
Size: 5267
Acc65I G'GTAC_C
Cuts at: 2283 2283
Size: 5267
AclI AA'CG_TT
Cuts at: 5107 5107
Size: 5267
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 905 1885 1901 1928 3092 3524 4271 4539 905
Size: 980 16 27 1164 432 747 268 1633
Fragments arranged by size:
1633 1164 980 747 432 268 27 16
AfeI AGC'GCT
Cuts at: 1498 1498
Size: 5267
AflIII A'CryG_T
Cuts at: 758 2619 3723 4700 758
Size: 1861 1104 977 1325
Fragments arranged by size:
1861 1325 1104 977
AgeI A'CCGG_T
Cuts at: 3655 3655
Size: 5267
AlwNI CAG_nnn'CTG
Cuts at: 319 675 2572 4139 319
Size: 356 1897 1567 1447
Fragments arranged by size:
1897 1567 1447 356
ApaLI G'TGCA_C
Cuts at: 4037 4037
Size: 5267
ApoI r'AATT_y
Cuts at: 355 816 2234 2271 4620 4876 355
Size: 461 1418 37 2349 256 746
Fragments arranged by size:
2349 1418 746 461 256 37
AseI AT'TA_AT
Cuts at: 20 20
Size: 5267
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5267
AvaI C'yCGr_G
Cuts at: 1319 2287 1319
Size: 968 4299
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 921 954 921
Size: 33 5234
BamHI G'GATC_C
Cuts at: 2292 2292
Size: 5267
BanI G'GyrC_C
Cuts at: 262 544 1383 2283 2697 2945 2980 262
Size: 282 839 900 414 248 35 2549
Fragments arranged by size:
2549 900 839 414 282 248 35
BanII G_rGCy'C
Cuts at: 2281 3311 2281
Size: 1030 4237
BbeI G_GCGC'C
Cuts at: 2701 2949 2701
Size: 248 5019
BbsI GAAGACnn'nnnn_
Cuts at: 1889 1889
Size: 5267
BbvCI CC'TCA_GC
Cuts at: 2113 2113
Size: 5267
BciVI GTATCCnnnnn_n'
Cuts at: 214 1064 3165 3926 5267 214
Size: 850 2101 761 1341 214
Fragments arranged by size:
2101 1341 850 761 214
BclI T'GATC_A
Cuts at: 683 1367 2787 683
Size: 684 1420 3163
Fragments arranged by size:
3163 1420 684
BglI GCCn_nnn'nGGC
Cuts at: 145 145
Size: 5267
BglII A'GATC_T
Cuts at: 2782 4819 2782
Size: 2037 3230
BlpI GC'TnA_GC
Cuts at: 2379 2379
Size: 5267
Bme1580I G_kGCm'C
Cuts at: 549 696 1587 2892 2985 4041 549
Size: 147 891 1305 93 1056 1775
Fragments arranged by size:
1775 1305 1056 891 147 93
BmrI ACTGGGnnnn_n'
Cuts at: 1453 1945 2479 2560 2895 5039 1453
Size: 492 534 81 335 2144 1681
Fragments arranged by size:
2144 1681 534 492 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1797 3634 1797
Size: 1837 3430
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 341 341
Size: 5267
Bpu10I CC'TnA_GC
Cuts at: 2113 2113
Size: 5267
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2443 3322 3814 4112 2443
Size: 879 492 298 3598
Fragments arranged by size:
3598 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1840 2126 4971 1840
Size: 286 2845 2136
Fragments arranged by size:
2845 2136 286
BsaAI yAC'GTr
Cuts at: 2620 2620
Size: 5267
BsaBI GATnn'nnATC
Cuts at: 6 1916 2781 4818 6
Size: 1910 865 2037 455
Fragments arranged by size:
2037 1910 865 455
BsaHI Gr'CG_yC
Cuts at: 2698 2946 3648 5144 2698
Size: 248 702 1496 2821
Fragments arranged by size:
2821 1496 702 248
BsaWI w'CCGG_w
Cuts at: 413 631 2456 2977 3655 3929 4076 4487 413
Size: 218 1825 521 678 274 147 411 1193
Fragments arranged by size:
1825 1193 678 521 411 274 218 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 626 656 1695 1725 1984 2014 626
Size: 30 1039 30 259 30 3879
Fragments arranged by size:
3879 1039 259 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1954 2136 2161 1954
Size: 182 25 5060
Fragments arranged by size:
5060 182 25
BseYI C'CCAG_C
Cuts at: 915 2692 3450 4027 4523 915
Size: 1777 758 577 496 1659
Fragments arranged by size:
1777 1659 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 976 976
Size: 5267
BsiEI CG_ry'CG
Cuts at: 104 2809 2855 4063 104
Size: 2705 46 1208 1308
Fragments arranged by size:
2705 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 299 1112 2281 3059 3249 4041 299
Size: 813 1169 778 190 792 1525
Fragments arranged by size:
1525 1169 813 792 778 190
BsmI GAATG_Cn'
Cuts at: 1820 1820
Size: 5267
Bsp1286I G_dGCh'C
Cuts at: 299 549 696 1112 1587 2281 2892 2985 3059
Size: 250 147 416 475 694 611 93 74
Cuts at: 3059 3249 3311 4041 299
Size: 190 62 730 1525
Fragments arranged by size:
1525 730 694 611 475 416 250 190
147 93 74 62
BspEI T'CCGG_A
Cuts at: 4487 4487
Size: 5267
BspHI T'CATG_A
Cuts at: 680 4443 680
Size: 3763 1504
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1939 2317 2531 2833 3214 1939
Size: 378 214 302 381 3992
Fragments arranged by size:
3992 381 378 302 214
BsrBI CCG'CTC
Cuts at: 50 3559 3613 5097 5262 50
Size: 3509 54 1484 165 55
Fragments arranged by size:
3509 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 134 980 1509 3179 134
Size: 846 529 1670 2222
Fragments arranged by size:
2222 1670 846 529
BsrFI r'CCGG_y
Cuts at: 3265 3446 3655 3265
Size: 181 209 4877
Fragments arranged by size:
4877 209 181
BsrGI T'GTAC_A
Cuts at: 1289 1289
Size: 5267
BssSI C'ACGA_G
Cuts at: 3538 3896 3538
Size: 358 4909
BstAPI GCAn_nnn'nTGC
Cuts at: 136 675 1116 2644 136
Size: 539 441 1528 2759
Fragments arranged by size:
2759 1528 539 441
BstBI TT'CG_AA
Cuts at: 1731 3628 1731
Size: 1897 3370
BstEII G'GTnAC_C
Cuts at: 1926 1926
Size: 5267
BstYI r'GATC_y
Cuts at: 1 316 531 2292 2759 2782 3117 3363 4375
Size: 315 215 1761 467 23 335 246 1012
Cuts at: 4375 4461 4473 4819 1
Size: 86 12 346 449
Fragments arranged by size:
1761 1012 467 449 346 335 315 246
215 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1962 1962
Size: 5267
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3217 3398 3561 3217
Size: 181 163 4923
Fragments arranged by size:
4923 181 163
BtsI GCAGTG_nn'
Cuts at: 347 653 1110 1220 1839 2510 347
Size: 306 457 110 619 671 3104
Fragments arranged by size:
3104 671 619 457 306 110
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1376 1411 1376
Size: 35 5232
DraI TTT'AAA
Cuts at: 2100 2266 2100
Size: 166 5101
DrdI GACnn_nn'nnGTC
Cuts at: 2973 3831 2973
Size: 858 4409
EaeI y'GGCC_r
Cuts at: 462 1649 2852 3026 3417 3444 4789 462
Size: 1187 1203 174 391 27 1345 940
Fragments arranged by size:
1345 1203 1187 940 391 174 27
EagI C'GGCC_G
Cuts at: 2852 2852
Size: 5267
EarI CTCTTCn'nnn_
Cuts at: 2138 2168 3290 3500 2138
Size: 30 1122 210 3905
Fragments arranged by size:
3905 1122 210 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3475 3785 3931 5077 3475
Size: 310 146 1146 3665
Fragments arranged by size:
3665 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2279 2279
Size: 5267
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 341 905 1885 1901 1928 3092 3524 4271 4539
Size: 564 980 16 27 1164 432 747 268
Cuts at: 4539 341
Size: 1069
Fragments arranged by size:
1164 1069 980 747 564 432 268 27
16
EcoO109I rG'GnC_Cy
Cuts at: 963 1896 2406 963
Size: 933 510 3824
Fragments arranged by size:
3824 933 510
EcoRI G'AATT_C
Cuts at: 355 2271 355
Size: 1916 3351
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3286 3286
Size: 5267
FspI TGC'GCA
Cuts at: 3048 3048
Size: 5267
HaeII r_GCGC'y
Cuts at: 240 1500 2701 2949 3971 4950 240
Size: 1260 1201 248 1022 979 557
Fragments arranged by size:
1260 1201 1022 979 557 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1212 1231 1244 1263 1984 2016 3109 3139 3141
Size: 19 13 19 721 32 1093 30 2
Cuts at: 3141 3171 1212
Size: 30 3308
Fragments arranged by size:
3308 1093 721 32 30 30 19 19
13 2
HincII GTy'rAC
Cuts at: 1992 2306 1992
Size: 314 4953
KasI G'GCGC_C
Cuts at: 2697 2945 2697
Size: 248 5019
KpnI G_GTAC'C
Cuts at: 2287 2287
Size: 5267
MfeI C'AATT_G
Cuts at: 257 257
Size: 5267
MluI A'CGCG_T
Cuts at: 4700 4700
Size: 5267
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 553 833 892 3938 4122 553
Size: 280 59 3046 184 1698
Fragments arranged by size:
3046 1698 280 184 59
MscI TGG'CCA
Cuts at: 464 1651 3028 464
Size: 1187 1377 2703
Fragments arranged by size:
2703 1377 1187
MslI CAynn'nnrTG
Cuts at: 607 1120 3256 3383 607
Size: 513 2136 127 2491
Fragments arranged by size:
2491 2136 513 127
MspA1I CmG'CkG
Cuts at: 2378 2692 3052 4065 4310 2378
Size: 314 360 1013 245 3335
Fragments arranged by size:
3335 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3448 3448
Size: 5267
NarI GG'CG_CC
Cuts at: 2698 2946 2698
Size: 248 5019
NgoMIV G'CCGG_C
Cuts at: 3446 3446
Size: 5267
NspI r_CATG'y
Cuts at: 762 1119 2320 2820 3727 762
Size: 357 1201 500 907 2302
Fragments arranged by size:
2302 1201 907 500 357
PciI A'CATG_T
Cuts at: 758 3723 758
Size: 2965 2302
PflMI CCAn_nnn'nTGG
Cuts at: 1745 1745
Size: 5267
PfoI T'CCnGG_A
Cuts at: 557 1066 557
Size: 509 4758
PmeI GTTT'AAAC
Cuts at: 2266 2266
Size: 5267
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4406 4438 4406
Size: 32 5235
PpuMI rG'GwC_Cy
Cuts at: 963 963
Size: 5267
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2059 2091 2059
Size: 32 5235
PstI C_TGCA'G
Cuts at: 671 1936 2314 2999 671
Size: 1265 378 685 2939
Fragments arranged by size:
2939 1265 685 378
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5267
PvuII CAG'CTG
Cuts at: 2692 3052 2692
Size: 360 4907
RsrII CG'GwC_CG
Cuts at: 3462 3462
Size: 5267
SacI G_AGCT'C
Cuts at: 2281 2281
Size: 5267
SalI G'TCGA_C
Cuts at: 2304 2304
Size: 5267
SapI GCTCTTCn'nnn_
Cuts at: 3290 3500 3290
Size: 210 5057
SbfI CC_TGCA'GG
Cuts at: 2314 2314
Size: 5267
ScaI AGT'ACT
Cuts at: 4784 4784
Size: 5267
SexAI A'CCwGG_T
Cuts at: 1760 1760
Size: 5267
SfcI C'TryA_G
Cuts at: 33 667 1932 2310 2995 3988 4179 33
Size: 634 1265 378 685 993 191 1121
Fragments arranged by size:
1265 1121 993 685 634 378 191
SfoI GGC'GCC
Cuts at: 2699 2947 2699
Size: 248 5019
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5267
SmaI CCC'GGG
Cuts at: 1321 2289 1321
Size: 968 4299
SmlI C'TyrA_G
Cuts at: 2422 3337 3829 4091 2422
Size: 915 492 262 3598
Fragments arranged by size:
3598 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5267
SphI G_CATG'C
Cuts at: 1119 2320 2820 1119
Size: 1201 500 3566
Fragments arranged by size:
3566 1201 500
SspI AAT'ATT
Cuts at: 285 1152 2205 285
Size: 867 1053 3347
Fragments arranged by size:
3347 1053 867
StuI AGG'CCT
Cuts at: 638 638
Size: 5267
StyI C'CwwG_G
Cuts at: 886 2401 2755 886
Size: 1515 354 3398
Fragments arranged by size:
3398 1515 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1371 2014 2850 2983 3442 3651 1371
Size: 643 836 133 459 209 2987
Fragments arranged by size:
2987 836 643 459 209 133
TatI w'GTAC_w
Cuts at: 117 1289 4782 117
Size: 1172 3493 602
Fragments arranged by size:
3493 1172 602
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 326 358 1695 1727 4784 4816 326
Size: 32 1337 32 3057 32 777
Fragments arranged by size:
3057 1337 777 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 946 3064 946
Size: 2118 3149
XbaI T'CTAG_A
Cuts at: 2298 2298
Size: 5267
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 525 923 2762 525
Size: 398 1839 3030
Fragments arranged by size:
3030 1839 398
XmaI C'CCGG_G
Cuts at: 1319 2287 1319
Size: 968 4299
XmnI GAAnn'nnTTC
Cuts at: 881 1062 1727 881
Size: 181 665 4421
Fragments arranged by size:
4421 665 181
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI
PflMI PfoI PmeI PpiI PpuMI PsrI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BcgI BfrBI BmgBI BmtI BsiWI BsmBI
BssHII BstXI BstZ17I BtgI ClaI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PmlI PshAI PsiI PspOMI
PspXI SacII SanDI SfiI SgrAI SnaBI SrfI SwaI
XhoI ZraI