Restriction map for
pF1KE0350
(Circular) (Six-base) MAPSORT of: pF1KE0350.seq Check: 549 from: 1 to: 5354
pF1KE0350 5354 bp
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AccI GT'mk_AC
Cuts at: 2047 2392 2047
Size: 345 5009
Acc65I G'GTAC_C
Cuts at: 2370 2370
Size: 5354
AclI AA'CG_TT
Cuts at: 5194 5194
Size: 5354
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 152 209 919 2005 3179 3611 4358 4626 152
Size: 57 710 1086 1174 432 747 268 880
Fragments arranged by size:
1174 1086 880 747 710 432 268 57
AflIII A'CryG_T
Cuts at: 1670 1748 2706 3810 4787 1670
Size: 78 958 1104 977 2237
Fragments arranged by size:
2237 1104 977 958 78
AgeI A'CCGG_T
Cuts at: 3742 3742
Size: 5354
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1047 1047
Size: 5354
AlwNI CAG_nnn'CTG
Cuts at: 2659 4226 2659
Size: 1567 3787
ApaLI G'TGCA_C
Cuts at: 4124 4124
Size: 5354
ApoI r'AATT_y
Cuts at: 840 1515 1896 1943 2180 2358 4707 4963 840
Size: 675 381 47 237 178 2349 256 1231
Fragments arranged by size:
2349 1231 675 381 256 237 178 47
AseI AT'TA_AT
Cuts at: 20 1301 20
Size: 1281 4073
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5354
AvaI C'yCGr_G
Cuts at: 2374 2374
Size: 5354
BamHI G'GATC_C
Cuts at: 2379 2379
Size: 5354
BanI G'GyrC_C
Cuts at: 893 2370 2784 3032 3067 893
Size: 1477 414 248 35 3180
Fragments arranged by size:
3180 1477 414 248 35
BanII G_rGCy'C
Cuts at: 1379 1842 2087 2368 3398 1379
Size: 463 245 281 1030 3335
Fragments arranged by size:
3335 1030 463 281 245
BbeI G_GCGC'C
Cuts at: 897 2788 3036 897
Size: 1891 248 3215
Fragments arranged by size:
3215 1891 248
BbsI GAAGACnn'nnnn_
Cuts at: 352 1291 1602 2112 352
Size: 939 311 510 3594
Fragments arranged by size:
3594 939 510 311
BbvCI CC'TCA_GC
Cuts at: 2327 2327
Size: 5354
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5320
BciVI GTATCCnnnnn_n'
Cuts at: 3252 4013 5354 3252
Size: 761 1341 3252
Fragments arranged by size:
3252 1341 761
BclI T'GATC_A
Cuts at: 2874 2874
Size: 5354
BfrBI ATG'CAT
Cuts at: 542 1158 542
Size: 616 4738
BglII A'GATC_T
Cuts at: 242 2869 4906 242
Size: 2627 2037 690
Fragments arranged by size:
2627 2037 690
BlpI GC'TnA_GC
Cuts at: 1373 2128 2466 1373
Size: 755 338 4261
Fragments arranged by size:
4261 755 338
Bme1580I G_kGCm'C
Cuts at: 537 2979 3072 4128 537
Size: 2442 93 1056 1763
Fragments arranged by size:
2442 1763 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2566 2647 2982 5126 2566
Size: 81 335 2144 2794
Fragments arranged by size:
2794 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3721 3721
Size: 5354
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 1564 1856 310
Size: 1254 292 3808
Fragments arranged by size:
3808 1254 292
Bpu10I CC'TnA_GC
Cuts at: 794 2327 794
Size: 1533 3821
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 848 1600 2245 2530 3409 3901 4199 848
Size: 752 645 285 879 492 298 2003
Fragments arranged by size:
2003 879 752 645 492 298 285
BsaI GGTCTCn'nnnn_
Cuts at: 5058 5058
Size: 5354
BsaAI yAC'GTr
Cuts at: 547 2707 547
Size: 2160 3194
BsaBI GATnn'nnATC
Cuts at: 6 2868 4905 6
Size: 2862 2037 455
Fragments arranged by size:
2862 2037 455
BsaHI Gr'CG_yC
Cuts at: 894 1203 2785 3033 3735 5231 894
Size: 309 1582 248 702 1496 1017
Fragments arranged by size:
1582 1496 1017 702 309 248
BsaWI w'CCGG_w
Cuts at: 2252 2543 3064 3742 4016 4163 4574 2252
Size: 291 521 678 274 147 411 3032
Fragments arranged by size:
3032 678 521 411 291 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 283 313 1345 1375 1660 1690 283
Size: 30 1032 30 285 30 3947
Fragments arranged by size:
3947 1032 285 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 653 900 1416 1668 2206 653
Size: 247 516 252 538 3801
Fragments arranged by size:
3801 538 516 252 247
BseYI C'CCAG_C
Cuts at: 126 2779 3537 4114 4610 126
Size: 2653 758 577 496 870
Fragments arranged by size:
2653 870 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 481 2096 481
Size: 1615 3739
BsiEI CG_ry'CG
Cuts at: 104 2896 2942 4150 104
Size: 2792 46 1208 1308
Fragments arranged by size:
2792 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1772 2134 2368 3146 3336 4128 1772
Size: 362 234 778 190 792 2998
Fragments arranged by size:
2998 792 778 362 234 190
BsmI GAATG_Cn'
Cuts at: 1861 1861
Size: 5354
Bsp1286I G_dGCh'C
Cuts at: 537 1379 1772 1842 2087 2134 2368 2979 3072
Size: 842 393 70 245 47 234 611 93
Cuts at: 3072 3146 3336 3398 4128 537
Size: 74 190 62 730 1763
Fragments arranged by size:
1763 842 730 611 393 245 234 190
93 74 70 62 47
BspEI T'CCGG_A
Cuts at: 2252 4574 2252
Size: 2322 3032
BspHI T'CATG_A
Cuts at: 261 1853 1865 2187 4530 261
Size: 1592 12 322 2343 1085
Fragments arranged by size:
2343 1592 1085 322 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2404 2618 2920 3301 2404
Size: 214 302 381 4457
Fragments arranged by size:
4457 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3646 3700 5184 5349 50
Size: 3596 54 1484 165 55
Fragments arranged by size:
3596 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 161 3266 161
Size: 3105 2249
BsrFI r'CCGG_y
Cuts at: 890 3352 3533 3742 890
Size: 2462 181 209 2502
Fragments arranged by size:
2502 2462 209 181
BssSI C'ACGA_G
Cuts at: 3625 3983 3625
Size: 358 4996
BstAPI GCAn_nnn'nTGC
Cuts at: 1454 2731 1454
Size: 1277 4077
BstBI TT'CG_AA
Cuts at: 3715 3715
Size: 5354
BstYI r'GATC_y
Cuts at: 1 242 1490 2379 2846 2869 3204 3450 4462
Size: 241 1248 889 467 23 335 246 1012
Cuts at: 4462 4548 4560 4906 1
Size: 86 12 346 449
Fragments arranged by size:
1248 1012 889 467 449 346 335 246
241 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1935 3304 3485 3648 1935
Size: 1369 181 163 3641
Fragments arranged by size:
3641 1369 181 163
BtsI GCAGTG_nn'
Cuts at: 1452 2179 2597 1452
Size: 727 418 4209
Fragments arranged by size:
4209 727 418
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1644 1679 1644
Size: 35 5319
DraI TTT'AAA
Cuts at: 1901 2353 1901
Size: 452 4902
DraIII CAC_nnn'GTG
Cuts at: 373 373
Size: 5354
DrdI GACnn_nn'nnGTC
Cuts at: 1096 3060 3918 1096
Size: 1964 858 2532
Fragments arranged by size:
2532 1964 858
EaeI y'GGCC_r
Cuts at: 1395 1665 2939 3113 3504 3531 4876 1395
Size: 270 1274 174 391 27 1345 1873
Fragments arranged by size:
1873 1345 1274 391 270 174 27
EagI C'GGCC_G
Cuts at: 2939 2939
Size: 5354
EarI CTCTTCn'nnn_
Cuts at: 128 3377 3587 128
Size: 3249 210 1895
Fragments arranged by size:
3249 1895 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3562 3872 4018 5164 3562
Size: 310 146 1146 3752
Fragments arranged by size:
3752 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2366 2366
Size: 5354
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 152 209 310 919 1564 1856 2005 3179 3611
Size: 57 101 609 645 292 149 1174 432
Cuts at: 3611 4358 4626 152
Size: 747 268 880
Fragments arranged by size:
1174 880 747 645 609 432 292 268
149 101 57
EcoNI CCTnn'n_nnAGG
Cuts at: 1351 1783 1351
Size: 432 4922
EcoO109I rG'GnC_Cy
Cuts at: 684 868 1272 2233 2493 684
Size: 184 404 961 260 3545
Fragments arranged by size:
3545 961 404 260 184
EcoRI G'AATT_C
Cuts at: 2358 2358
Size: 5354
EcoRV GAT'ATC
Cuts at: 1881 2250 1881
Size: 369 4985
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3373 3373
Size: 5354
FspI TGC'GCA
Cuts at: 160 3135 160
Size: 2975 2379
HaeII r_GCGC'y
Cuts at: 897 2788 3036 4058 5037 897
Size: 1891 248 1022 979 1214
Fragments arranged by size:
1891 1214 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 243 275 283 315 466 498 633 665 1636
Size: 32 8 32 151 32 135 32 971
Cuts at: 1636 1668 1867 1899 3196 3226 3228 3258 243
Size: 32 199 32 1297 30 2 30 2339
Fragments arranged by size:
2339 1297 971 199 151 135 32 32
32 32 32 32 30 30 8 2
HincII GTy'rAC
Cuts at: 2393 2393
Size: 5354
KasI G'GCGC_C
Cuts at: 893 2784 3032 893
Size: 1891 248 3215
Fragments arranged by size:
3215 1891 248
KpnI G_GTAC'C
Cuts at: 2374 2374
Size: 5354
MluI A'CGCG_T
Cuts at: 4787 4787
Size: 5354
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 451 1839 4025 4209 451
Size: 1388 2186 184 1596
Fragments arranged by size:
2186 1596 1388 184
MscI TGG'CCA
Cuts at: 1667 3115 1667
Size: 1448 3906
MslI CAynn'nnrTG
Cuts at: 404 1019 1163 1561 1765 1954 3343 3470 404
Size: 615 144 398 204 189 1389 127 2288
Fragments arranged by size:
2288 1389 615 398 204 189 144 127
MspA1I CmG'CkG
Cuts at: 130 2465 2779 3139 4152 4397 130
Size: 2335 314 360 1013 245 1087
Fragments arranged by size:
2335 1087 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3535 3535
Size: 5354
NarI GG'CG_CC
Cuts at: 894 2785 3033 894
Size: 1891 248 3215
Fragments arranged by size:
3215 1891 248
NgoMIV G'CCGG_C
Cuts at: 3533 3533
Size: 5354
NsiI A_TGCA'T
Cuts at: 544 1160 544
Size: 616 4738
NspI r_CATG'y
Cuts at: 561 1372 1674 1752 2407 2907 3814 561
Size: 811 302 78 655 500 907 2101
Fragments arranged by size:
2101 907 811 655 500 302 78
PciI A'CATG_T
Cuts at: 1670 1748 3810 1670
Size: 78 2062 3214
Fragments arranged by size:
3214 2062 78
PflMI CCAn_nnn'nTGG
Cuts at: 1531 1531
Size: 5354
PmeI GTTT'AAAC
Cuts at: 2353 2353
Size: 5354
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4493 4525 4493
Size: 32 5322
PpuMI rG'GwC_Cy
Cuts at: 684 868 1272 684
Size: 184 404 4766
Fragments arranged by size:
4766 404 184
PsiI TTA'TAA
Cuts at: 992 992
Size: 5354
PstI C_TGCA'G
Cuts at: 1000 2401 3086 1000
Size: 1401 685 3268
Fragments arranged by size:
3268 1401 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5354
PvuII CAG'CTG
Cuts at: 130 2779 3139 130
Size: 2649 360 2345
Fragments arranged by size:
2649 2345 360
RsrII CG'GwC_CG
Cuts at: 3549 3549
Size: 5354
SacI G_AGCT'C
Cuts at: 2368 2368
Size: 5354
SalI G'TCGA_C
Cuts at: 2391 2391
Size: 5354
SapI GCTCTTCn'nnn_
Cuts at: 3377 3587 3377
Size: 210 5144
SbfI CC_TGCA'GG
Cuts at: 2401 2401
Size: 5354
ScaI AGT'ACT
Cuts at: 1924 4871 1924
Size: 2947 2407
SexAI A'CCwGG_T
Cuts at: 1346 2299 1346
Size: 953 4401
SfcI C'TryA_G
Cuts at: 33 996 1101 1250 1918 2048 2397 3082 4075
Size: 963 105 149 668 130 349 685 993
Cuts at: 4075 4266 33
Size: 191 1121
Fragments arranged by size:
1121 993 963 685 668 349 191 149
130 105
SfoI GGC'GCC
Cuts at: 895 2786 3034 895
Size: 1891 248 3215
Fragments arranged by size:
3215 1891 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5354
SgrAI Cr'CCGG_yG
Cuts at: 890 890
Size: 5354
SmaI CCC'GGG
Cuts at: 2376 2376
Size: 5354
SmlI C'TyrA_G
Cuts at: 863 1615 2224 2509 3424 3916 4178 863
Size: 752 609 285 915 492 262 2039
Fragments arranged by size:
2039 915 752 609 492 285 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5354
SphI G_CATG'C
Cuts at: 1372 2407 2907 1372
Size: 1035 500 3819
Fragments arranged by size:
3819 1035 500
SspI AAT'ATT
Cuts at: 1312 1551 1312
Size: 239 5115
StyI C'CwwG_G
Cuts at: 336 2078 2488 2842 336
Size: 1742 410 354 2848
Fragments arranged by size:
2848 1742 410 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1027 1861 2937 3070 3529 3738 1027
Size: 834 1076 133 459 209 2643
Fragments arranged by size:
2643 1076 834 459 209 133
TatI w'GTAC_w
Cuts at: 1922 4869 1922
Size: 2947 2407
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 266 298 1660 1692 4871 4903 266
Size: 32 1362 32 3179 32 717
Fragments arranged by size:
3179 1362 717 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 297 3151 297
Size: 2854 2500
XbaI T'CTAG_A
Cuts at: 1986 2385 1986
Size: 399 4955
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2849 2849
Size: 5354
XmaI C'CCGG_G
Cuts at: 2374 2374
Size: 5354
XmnI GAAnn'nnTTC
Cuts at: 1552 1552
Size: 5354
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgZI BtsI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NsiI NspI PciI
PflMI PmeI PpiI PpuMI PsiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BglI BmgBI BmtI BsiWI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI PacI PasI PfoI PmlI PshAI PspOMI
PspXI PsrI SacII SanDI SfiI SnaBI SrfI StuI
SwaI XhoI ZraI