Restriction map for
pF1KE0351
(Circular) (Six-base) MAPSORT of: pF1KE0351.seq Check: 3818 from: 1 to: 5567
pF1KE0351 5567 bp
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 993 2404 993
Size: 1411 4156
AccI GT'mk_AC
Cuts at: 262 2605 262
Size: 2343 3224
Acc65I G'GTAC_C
Cuts at: 821 1065 2583 821
Size: 244 1518 3805
Fragments arranged by size:
3805 1518 244
AclI AA'CG_TT
Cuts at: 5407 5407
Size: 5567
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 694 1042 1161 1600 2539 3392 3824 4571 4839
Size: 348 119 439 939 853 432 747 268
Cuts at: 4839 694
Size: 1422
Fragments arranged by size:
1422 939 853 747 439 432 348 268
119
AflIII A'CryG_T
Cuts at: 2919 4023 5000 2919
Size: 1104 977 3486
Fragments arranged by size:
3486 1104 977
AgeI A'CCGG_T
Cuts at: 3955 3955
Size: 5567
AleI CACnn'nnGTG
Cuts at: 631 631
Size: 5567
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 942 942
Size: 5567
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 502 534 502
Size: 32 5535
AlwNI CAG_nnn'CTG
Cuts at: 721 1032 1149 1440 2505 2872 4439 721
Size: 311 117 291 1065 367 1567 1849
Fragments arranged by size:
1849 1567 1065 367 311 291 117
ApaI G_GGCC'C
Cuts at: 2184 2184
Size: 5567
ApaLI G'TGCA_C
Cuts at: 946 4337 946
Size: 3391 2176
ApoI r'AATT_y
Cuts at: 2571 4920 5176 2571
Size: 2349 256 2962
Fragments arranged by size:
2962 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5567
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5567
AvaI C'yCGr_G
Cuts at: 581 1103 1271 1809 2183 2201 2272 2587 581
Size: 522 168 538 374 18 71 315 3561
Fragments arranged by size:
3561 538 522 374 315 168 71 18
BamHI G'GATC_C
Cuts at: 1649 2592 1649
Size: 943 4624
BanI G'GyrC_C
Cuts at: 770 821 1065 1099 2488 2583 2997 3245 3280
Size: 51 244 34 1389 95 414 248 35
Cuts at: 3280 770
Size: 3057
Fragments arranged by size:
3057 1389 414 248 244 95 51 35
34
BanII G_rGCy'C
Cuts at: 784 1610 1661 1799 2030 2184 2363 2581 3611
Size: 826 51 138 231 154 179 218 1030
Cuts at: 3611 784
Size: 2740
Fragments arranged by size:
2740 1030 826 231 218 179 154 138
51
BbeI G_GCGC'C
Cuts at: 774 3001 3249 774
Size: 2227 248 3092
Fragments arranged by size:
3092 2227 248
BbsI GAAGACnn'nnnn_
Cuts at: 2441 2441
Size: 5567
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2271 2305 2271
Size: 34 5533
BciVI GTATCCnnnnn_n'
Cuts at: 3465 4226 5567 3465
Size: 761 1341 3465
Fragments arranged by size:
3465 1341 761
BclI T'GATC_A
Cuts at: 3087 3087
Size: 5567
BglI GCCn_nnn'nGGC
Cuts at: 840 840
Size: 5567
BglII A'GATC_T
Cuts at: 3082 5119 3082
Size: 2037 3530
BlpI GC'TnA_GC
Cuts at: 376 763 1355 2679 376
Size: 387 592 1324 3264
Fragments arranged by size:
3264 1324 592 387
Bme1580I G_kGCm'C
Cuts at: 715 836 950 1104 2184 2556 3192 3285 4341
Size: 121 114 154 1080 372 636 93 1056
Cuts at: 4341 715
Size: 1941
Fragments arranged by size:
1941 1080 1056 636 372 154 121 114
93
BmrI ACTGGGnnnn_n'
Cuts at: 2779 2860 3195 5339 2779
Size: 81 335 2144 3007
Fragments arranged by size:
3007 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3934 3934
Size: 5567
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 391 676 682 768 1270 1318 1363 1423
Size: 177 285 6 86 502 48 45 60
Cuts at: 1423 1543 1707 1849 1909 1918 2347 214
Size: 120 164 142 60 9 429 3434
Fragments arranged by size:
3434 502 429 285 177 164 142 120
86 60 60 48 45 9 6
Bpu10I CC'TnA_GC
Cuts at: 988 1604 988
Size: 616 4951
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 898 1515 2743 3622 4114 4412 898
Size: 617 1228 879 492 298 2053
Fragments arranged by size:
2053 1228 879 617 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1040 5271 1040
Size: 4231 1336
BsaAI yAC'GTr
Cuts at: 246 1199 2920 246
Size: 953 1721 2893
Fragments arranged by size:
2893 1721 953
BsaBI GATnn'nnATC
Cuts at: 6 605 3081 5118 6
Size: 599 2476 2037 455
Fragments arranged by size:
2476 2037 599 455
BsaHI Gr'CG_yC
Cuts at: 771 2048 2998 3246 3948 5444 771
Size: 1277 950 248 702 1496 894
Fragments arranged by size:
1496 1277 950 894 702 248
BsaWI w'CCGG_w
Cuts at: 467 2525 2756 3277 3955 4229 4376 4787 467
Size: 2058 231 521 678 274 147 411 1247
Fragments arranged by size:
2058 1247 678 521 411 274 231 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 103 133 1684 1714 1822 1852 2058 2088 103
Size: 30 1551 30 108 30 206 30 3582
Fragments arranged by size:
3582 1551 206 108 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 514 517 792 949 988 1109 1659 1809 514
Size: 3 275 157 39 121 550 150 4272
Fragments arranged by size:
4272 550 275 157 150 121 39 3
BseYI C'CCAG_C
Cuts at: 212 817 1149 2005 2212 2992 3750 4327 4823
Size: 605 332 856 207 780 758 577 496
Cuts at: 4823 212
Size: 956
Fragments arranged by size:
956 856 780 758 605 577 496 332
207
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 989 1399 1924 2275 2494 989
Size: 410 525 351 219 4062
Fragments arranged by size:
4062 525 410 351 219
BsiEI CG_ry'CG
Cuts at: 104 266 3109 3155 4363 104
Size: 162 2843 46 1208 1308
Fragments arranged by size:
2843 1308 1208 162 46
BsiHKAI G_wGCw'C
Cuts at: 628 784 950 1280 1610 2363 2581 3359 3549
Size: 156 166 330 330 753 218 778 190
Cuts at: 3549 4341 628
Size: 792 1854
Fragments arranged by size:
1854 792 778 753 330 330 218 190
166 156
BsmI GAATG_Cn'
Cuts at: 2248 2248
Size: 5567
Bsp1286I G_dGCh'C
Cuts at: 628 715 784 836 950 1104 1280 1610 1661
Size: 87 69 52 114 154 176 330 51
Cuts at: 1661 1799 2030 2184 2363 2556 2581 3192 3285
Size: 138 231 154 179 193 25 611 93
Cuts at: 3285 3359 3549 3611 4341 628
Size: 74 190 62 730 1854
Fragments arranged by size:
1854 730 611 330 231 193 190 179
176 154 154 138 114 93 87 74
69 62 52 51 25
BspEI T'CCGG_A
Cuts at: 467 4787 467
Size: 4320 1247
BspHI T'CATG_A
Cuts at: 4743 4743
Size: 5567
BspMI ACCTGCnnnn'nnnn_
Cuts at: 175 215 993 1058 1718 2404 2617 2831 3133
Size: 40 778 65 660 686 213 214 302
Cuts at: 3133 3514 175
Size: 381 2228
Fragments arranged by size:
2228 778 686 660 381 302 214 213
65 40
BsrBI CCG'CTC
Cuts at: 50 767 3859 3913 5397 5562 50
Size: 717 3092 54 1484 165 55
Fragments arranged by size:
3092 1484 717 165 55 54
BsrDI GCAATG_nn'
Cuts at: 798 3479 798
Size: 2681 2886
BsrFI r'CCGG_y
Cuts at: 3565 3746 3955 3565
Size: 181 209 5177
Fragments arranged by size:
5177 209 181
BssSI C'ACGA_G
Cuts at: 1836 3838 4196 1836
Size: 2002 358 3207
Fragments arranged by size:
3207 2002 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1032 2944 1032
Size: 1912 3655
BstBI TT'CG_AA
Cuts at: 3928 3928
Size: 5567
BstYI r'GATC_y
Cuts at: 1 1649 1664 2379 2592 3059 3082 3417 3663
Size: 1648 15 715 213 467 23 335 246
Cuts at: 3663 4675 4761 4773 5119 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1648 1012 715 467 449 346 335 246
213 86 23 15 12
Bsu36I CC'TnA_GG
Cuts at: 775 838 932 2219 2506 775
Size: 63 94 1287 287 3836
Fragments arranged by size:
3836 1287 287 94 63
BtgI C'CryG_G
Cuts at: 107 107
Size: 5567
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3517 3698 3861 3517
Size: 181 163 5223
Fragments arranged by size:
5223 181 163
BtsI GCAGTG_nn'
Cuts at: 2152 2810 2152
Size: 658 4909
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1359 1394 1359
Size: 35 5532
DraI TTT'AAA
Cuts at: 1875 2566 1875
Size: 691 4876
DrdI GACnn_nn'nnGTC
Cuts at: 3273 4131 3273
Size: 858 4709
EaeI y'GGCC_r
Cuts at: 138 941 1010 1085 1125 1283 1419 2089 2370
Size: 803 69 75 40 158 136 670 281
Cuts at: 2370 3152 3326 3717 3744 5089 138
Size: 782 174 391 27 1345 616
Fragments arranged by size:
1345 803 782 670 616 391 281 174
158 136 75 69 40 27
EagI C'GGCC_G
Cuts at: 3152 3152
Size: 5567
EarI CTCTTCn'nnn_
Cuts at: 1615 2257 3590 3800 1615
Size: 642 1333 210 3382
Fragments arranged by size:
3382 1333 642 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3775 4085 4231 5377 3775
Size: 310 146 1146 3965
Fragments arranged by size:
3965 1146 310 146
EcoICRI GAG'CTC
Cuts at: 782 1608 2361 2579 782
Size: 826 753 218 3770
Fragments arranged by size:
3770 826 753 218
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 391 676 682 694 768 1042 1161 1270
Size: 177 285 6 12 74 274 119 109
Cuts at: 1270 1318 1363 1423 1543 1600 1707 1849 1909
Size: 48 45 60 120 57 107 142 60
Cuts at: 1909 1918 2347 2539 3392 3824 4571 4839 214
Size: 9 429 192 853 432 747 268 942
Fragments arranged by size:
942 853 747 432 429 285 274 268
192 177 142 120 119 109 107 74
60 60 57 48 45 12 9 6
EcoNI CCTnn'n_nnAGG
Cuts at: 1024 1054 1024
Size: 30 5537
EcoO109I rG'GnC_Cy
Cuts at: 189 680 842 1017 1029 1224 1245 1255 1589
Size: 491 162 175 12 195 21 10 334
Cuts at: 1589 1814 1895 1929 2277 2502 2706 189
Size: 225 81 34 348 225 204 3050
Fragments arranged by size:
3050 491 348 334 225 225 204 195
175 162 81 34 21 12 10
EcoRI G'AATT_C
Cuts at: 2571 2571
Size: 5567
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3586 3586
Size: 5567
FspI TGC'GCA
Cuts at: 3348 3348
Size: 5567
HaeII r_GCGC'y
Cuts at: 774 1320 1396 1861 2448 3001 3249 4271 5250
Size: 546 76 465 587 553 248 1022 979
Cuts at: 5250 774
Size: 1091
Fragments arranged by size:
1091 1022 979 587 553 546 465 248
76
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 103 135 379 411 3409 3439 3441 3471 103
Size: 32 244 32 2998 30 2 30 2199
Fragments arranged by size:
2998 2199 244 32 32 30 30 2
HincII GTy'rAC
Cuts at: 263 2606 263
Size: 2343 3224
KasI G'GCGC_C
Cuts at: 770 2997 3245 770
Size: 2227 248 3092
Fragments arranged by size:
3092 2227 248
KpnI G_GTAC'C
Cuts at: 825 1069 2587 825
Size: 244 1518 3805
Fragments arranged by size:
3805 1518 244
MluI A'CGCG_T
Cuts at: 5000 5000
Size: 5567
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2438 4238 4422 2438
Size: 1800 184 3583
Fragments arranged by size:
3583 1800 184
MscI TGG'CCA
Cuts at: 140 1012 1087 1285 2372 3328 140
Size: 872 75 198 1087 956 2379
Fragments arranged by size:
2379 1087 956 872 198 75
MslI CAynn'nnrTG
Cuts at: 631 3556 3683 631
Size: 2925 127 2515
Fragments arranged by size:
2925 2515 127
MspA1I CmG'CkG
Cuts at: 258 631 817 1038 1938 2013 2301 2678 2992
Size: 373 186 221 900 75 288 377 314
Cuts at: 2992 3352 4365 4610 258
Size: 360 1013 245 1215
Fragments arranged by size:
1215 1013 900 377 373 360 314 288
245 221 186 75
NaeI GCC'GGC
Cuts at: 3748 3748
Size: 5567
NarI GG'CG_CC
Cuts at: 771 2998 3246 771
Size: 2227 248 3092
Fragments arranged by size:
3092 2227 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 5567
NgoMIV G'CCGG_C
Cuts at: 3746 3746
Size: 5567
NspI r_CATG'y
Cuts at: 2620 3120 4027 2620
Size: 500 907 4160
Fragments arranged by size:
4160 907 500
PasI CC'CwG_GG
Cuts at: 492 492
Size: 5567
PciI A'CATG_T
Cuts at: 4023 4023
Size: 5567
PflMI CCAn_nnn'nTGG
Cuts at: 741 1134 1428 741
Size: 393 294 4880
Fragments arranged by size:
4880 393 294
PfoI T'CCnGG_A
Cuts at: 191 1652 1750 191
Size: 1461 98 4008
Fragments arranged by size:
4008 1461 98
PmeI GTTT'AAAC
Cuts at: 2566 2566
Size: 5567
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4706 4738 4706
Size: 32 5535
PpuMI rG'GwC_Cy
Cuts at: 189 680 1224 1245 1255 1814 189
Size: 491 544 21 10 559 3942
Fragments arranged by size:
3942 559 544 491 21 10
PshAI GACnn'nnGTC
Cuts at: 308 308
Size: 5567
PspOMI G'GGCC_C
Cuts at: 2180 2180
Size: 5567
PstI C_TGCA'G
Cuts at: 911 1037 1055 1847 2159 2501 2614 3299 911
Size: 126 18 792 312 342 113 685 3179
Fragments arranged by size:
3179 792 685 342 312 126 113 18
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5567
PvuII CAG'CTG
Cuts at: 258 817 1038 1938 2013 2992 3352 258
Size: 559 221 900 75 979 360 2473
Fragments arranged by size:
2473 979 900 559 360 221 75
RsrII CG'GwC_CG
Cuts at: 3762 3762
Size: 5567
SacI G_AGCT'C
Cuts at: 784 1610 2363 2581 784
Size: 826 753 218 3770
Fragments arranged by size:
3770 826 753 218
SalI G'TCGA_C
Cuts at: 261 2604 261
Size: 2343 3224
SanDI GG'GwC_CC
Cuts at: 1224 1224
Size: 5567
SapI GCTCTTCn'nnn_
Cuts at: 1615 3590 3800 1615
Size: 1975 210 3382
Fragments arranged by size:
3382 1975 210
SbfI CC_TGCA'GG
Cuts at: 1055 2501 2614 1055
Size: 1446 113 4008
Fragments arranged by size:
4008 1446 113
ScaI AGT'ACT
Cuts at: 5084 5084
Size: 5567
SfcI C'TryA_G
Cuts at: 33 253 907 1033 1051 1843 1953 2155 2497
Size: 220 654 126 18 792 110 202 342
Cuts at: 2497 2610 3295 4288 4479 33
Size: 113 685 993 191 1121
Fragments arranged by size:
1121 993 792 685 654 342 220 202
191 126 113 110 18
SfoI GGC'GCC
Cuts at: 772 2999 3247 772
Size: 2227 248 3092
Fragments arranged by size:
3092 2227 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5567
SmaI CCC'GGG
Cuts at: 1105 1273 2185 2203 2589 1105
Size: 168 912 18 386 4083
Fragments arranged by size:
4083 912 386 168 18
SmlI C'TyrA_G
Cuts at: 913 1494 2722 3637 4129 4391 913
Size: 581 1228 915 492 262 2089
Fragments arranged by size:
2089 1228 915 581 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5567
SphI G_CATG'C
Cuts at: 2620 3120 2620
Size: 500 5067
SrfI GCCC'GGGC
Cuts at: 1105 1105
Size: 5567
StuI AGG'CCT
Cuts at: 160 331 982 1834 2544 160
Size: 171 651 852 710 3183
Fragments arranged by size:
3183 852 710 651 171
StyI C'CwwG_G
Cuts at: 107 1013 2021 2236 2548 2701 3055 107
Size: 906 1008 215 312 153 354 2619
Fragments arranged by size:
2619 1008 906 354 312 215 153
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3150 3283 3742 3951 3150
Size: 133 459 209 4766
Fragments arranged by size:
4766 459 209 133
TatI w'GTAC_w
Cuts at: 2171 5082 2171
Size: 2911 2656
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 297 329 1216 1248 1822 1854 2058 2090 5084
Size: 32 887 32 574 32 204 32 2994
Cuts at: 5084 5116 297
Size: 32 748
Fragments arranged by size:
2994 887 748 574 204 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 397 3364 397
Size: 2967 2600
XbaI T'CTAG_A
Cuts at: 2532 2598 2532
Size: 66 5501
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1293 1377 3062 1293
Size: 84 1685 3798
Fragments arranged by size:
3798 1685 84
XmaI C'CCGG_G
Cuts at: 1103 1271 2183 2201 2587 1103
Size: 168 912 18 386 4083
Fragments arranged by size:
4083 912 386 168 18
XmnI GAAnn'nnTTC
Cuts at: 475 475
Size: 5567
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PspOMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SrfI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AscI AvrII BaeI BbvCI
BfrBI BmgBI BmtI BsiWI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I ClaI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PmlI PsiI PspXI PsrI SacII SexAI SfiI SgrAI
SnaBI SspI SwaI XhoI ZraI