Restriction map for
pF1KE0352
(Circular) (Six-base) MAPSORT of: pF1KE0352.seq Check: 2255 from: 1 to: 4703
pF1KE0352 4703 bp
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AccI GT'mk_AC
Cuts at: 1118 1431 1741 1118
Size: 313 310 4080
Fragments arranged by size:
4080 313 310
Acc65I G'GTAC_C
Cuts at: 1719 1719
Size: 4703
AclI AA'CG_TT
Cuts at: 4543 4543
Size: 4703
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 1030 2528 2960 3707 3975 175
Size: 855 1498 432 747 268 903
Fragments arranged by size:
1498 903 855 747 432 268
AflIII A'CryG_T
Cuts at: 485 836 2055 3159 4136 485
Size: 351 1219 1104 977 1052
Fragments arranged by size:
1219 1104 1052 977 351
AgeI A'CCGG_T
Cuts at: 3091 3091
Size: 4703
AleI CACnn'nnGTG
Cuts at: 1024 1024
Size: 4703
AlwNI CAG_nnn'CTG
Cuts at: 2008 3575 2008
Size: 1567 3136
ApaLI G'TGCA_C
Cuts at: 3473 3473
Size: 4703
ApoI r'AATT_y
Cuts at: 265 448 1707 4056 4312 265
Size: 183 1259 2349 256 656
Fragments arranged by size:
2349 1259 656 256 183
AseI AT'TA_AT
Cuts at: 20 20
Size: 4703
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4703
AvaI C'yCGr_G
Cuts at: 136 380 1525 1723 136
Size: 244 1145 198 3116
Fragments arranged by size:
3116 1145 244 198
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1266 1299 1266
Size: 33 4670
BamHI G'GATC_C
Cuts at: 1728 1728
Size: 4703
BanI G'GyrC_C
Cuts at: 216 1719 2133 2381 2416 216
Size: 1503 414 248 35 2503
Fragments arranged by size:
2503 1503 414 248 35
BanII G_rGCy'C
Cuts at: 618 1717 2747 618
Size: 1099 1030 2574
Fragments arranged by size:
2574 1099 1030
BbeI G_GCGC'C
Cuts at: 220 2137 2385 220
Size: 1917 248 2538
Fragments arranged by size:
2538 1917 248
BbvCI CC'TCA_GC
Cuts at: 680 680
Size: 4703
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 114 148 1246 1280 114
Size: 34 1098 34 3537
Fragments arranged by size:
3537 1098 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 839 2601 3362 4703 839
Size: 1762 761 1341 839
Fragments arranged by size:
1762 1341 839 761
BclI T'GATC_A
Cuts at: 2223 2223
Size: 4703
BglI GCCn_nnn'nGGC
Cuts at: 613 613
Size: 4703
BglII A'GATC_T
Cuts at: 289 2218 4255 289
Size: 1929 2037 737
Fragments arranged by size:
2037 1929 737
BlpI GC'TnA_GC
Cuts at: 1815 1815
Size: 4703
Bme1580I G_kGCm'C
Cuts at: 609 2328 2421 3477 609
Size: 1719 93 1056 1835
Fragments arranged by size:
1835 1719 1056 93
BmgBI CAC'GTC
Cuts at: 1352 1352
Size: 4703
BmrI ACTGGGnnnn_n'
Cuts at: 822 1915 1996 2331 4475 822
Size: 1093 81 335 2144 1050
Fragments arranged by size:
2144 1093 1050 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 207 1429 3070 207
Size: 1222 1641 1840
Fragments arranged by size:
1840 1641 1222
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 707 707
Size: 4703
Bpu10I CC'TnA_GC
Cuts at: 518 680 518
Size: 162 4541
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1879 2758 3250 3548 1879
Size: 879 492 298 3034
Fragments arranged by size:
3034 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4407 4407
Size: 4703
BsaAI yAC'GTr
Cuts at: 2056 2056
Size: 4703
BsaBI GATnn'nnATC
Cuts at: 6 2217 4254 6
Size: 2211 2037 455
Fragments arranged by size:
2211 2037 455
BsaHI Gr'CG_yC
Cuts at: 179 217 456 2134 2382 3084 4580 179
Size: 38 239 1678 248 702 1496 302
Fragments arranged by size:
1678 1496 702 302 248 239 38
BsaWI w'CCGG_w
Cuts at: 1341 1892 2413 3091 3365 3512 3923 1341
Size: 551 521 678 274 147 411 2121
Fragments arranged by size:
2121 678 551 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 680 710 1100 1130 1336 1366 680
Size: 30 390 30 206 30 4017
Fragments arranged by size:
4017 390 206 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 163 163
Size: 4703
BseYI C'CCAG_C
Cuts at: 910 2128 2886 3463 3959 910
Size: 1218 758 577 496 1654
Fragments arranged by size:
1654 1218 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2245 2291 3499 104
Size: 2141 46 1208 1308
Fragments arranged by size:
2141 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 519 1149 1259 1717 2495 2685 3477 519
Size: 630 110 458 778 190 792 1745
Fragments arranged by size:
1745 792 778 630 458 190 110
Bsp1286I G_dGCh'C
Cuts at: 519 609 618 1149 1259 1717 2328 2421 2495
Size: 90 9 531 110 458 611 93 74
Cuts at: 2495 2685 2747 3477 519
Size: 190 62 730 1745
Fragments arranged by size:
1745 730 611 531 458 190 110 93
90 74 62 9
BspEI T'CCGG_A
Cuts at: 1341 3923 1341
Size: 2582 2121
BspHI T'CATG_A
Cuts at: 3879 3879
Size: 4703
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1753 1967 2269 2650 1753
Size: 214 302 381 3806
Fragments arranged by size:
3806 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2995 3049 4533 4698 50
Size: 2945 54 1484 165 55
Fragments arranged by size:
2945 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2615 2615
Size: 4703
BsrFI r'CCGG_y
Cuts at: 2701 2882 3091 2701
Size: 181 209 4313
Fragments arranged by size:
4313 209 181
BsrGI T'GTAC_A
Cuts at: 559 559
Size: 4703
BssSI C'ACGA_G
Cuts at: 2974 3332 2974
Size: 358 4345
BstAPI GCAn_nnn'nTGC
Cuts at: 1082 2080 1082
Size: 998 3705
BstBI TT'CG_AA
Cuts at: 3064 3064
Size: 4703
BstYI r'GATC_y
Cuts at: 1 289 848 1728 2195 2218 2553 2799 3811
Size: 288 559 880 467 23 335 246 1012
Cuts at: 3811 3897 3909 4255 1
Size: 86 12 346 449
Fragments arranged by size:
1012 880 559 467 449 346 335 288
246 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 139 2653 2834 2997 139
Size: 2514 181 163 1845
Fragments arranged by size:
2514 1845 181 163
BtsI GCAGTG_nn'
Cuts at: 701 1946 701
Size: 1245 3458
ClaI AT'CG_AT
Cuts at: 1319 1319
Size: 4703
DraI TTT'AAA
Cuts at: 1702 1702
Size: 4703
DraIII CAC_nnn'GTG
Cuts at: 604 604
Size: 4703
DrdI GACnn_nn'nnGTC
Cuts at: 2409 3267 2409
Size: 858 3845
EaeI y'GGCC_r
Cuts at: 200 282 929 1390 2288 2462 2853 2880 4225
Size: 82 647 461 898 174 391 27 1345
Cuts at: 4225 200
Size: 678
Fragments arranged by size:
1345 898 678 647 461 391 174 82
27
EagI C'GGCC_G
Cuts at: 2288 2288
Size: 4703
EarI CTCTTCn'nnn_
Cuts at: 860 1006 2726 2936 860
Size: 146 1720 210 2627
Fragments arranged by size:
2627 1720 210 146
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2911 3221 3367 4513 2911
Size: 310 146 1146 3101
Fragments arranged by size:
3101 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1715 1715
Size: 4703
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 707 1030 2528 2960 3707 3975 175
Size: 532 323 1498 432 747 268 903
Fragments arranged by size:
1498 903 747 532 432 323 268
EcoNI CCTnn'n_nnAGG
Cuts at: 1420 1420
Size: 4703
EcoO109I rG'GnC_Cy
Cuts at: 1842 1842
Size: 4703
EcoRI G'AATT_C
Cuts at: 1707 1707
Size: 4703
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 210 2722 210
Size: 2512 2191
FspI TGC'GCA
Cuts at: 2484 2484
Size: 4703
HaeII r_GCGC'y
Cuts at: 220 2137 2385 3407 4386 220
Size: 1917 248 1022 979 537
Fragments arranged by size:
1917 1022 979 537 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1100 1132 2545 2575 2577 2607 1100
Size: 32 1413 30 2 30 3196
Fragments arranged by size:
3196 1413 32 30 30 2
HincII GTy'rAC
Cuts at: 1742 1742
Size: 4703
KasI G'GCGC_C
Cuts at: 216 2133 2381 216
Size: 1917 248 2538
Fragments arranged by size:
2538 1917 248
KpnI G_GTAC'C
Cuts at: 1723 1723
Size: 4703
MfeI C'AATT_G
Cuts at: 1112 1112
Size: 4703
MluI A'CGCG_T
Cuts at: 4136 4136
Size: 4703
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1331 3374 3558 1331
Size: 2043 184 2476
Fragments arranged by size:
2476 2043 184
MscI TGG'CCA
Cuts at: 202 1392 2464 202
Size: 1190 1072 2441
Fragments arranged by size:
2441 1190 1072
MslI CAynn'nnrTG
Cuts at: 112 1024 2692 2819 112
Size: 912 1668 127 1996
Fragments arranged by size:
1996 1668 912 127
MspA1I CmG'CkG
Cuts at: 132 340 583 626 749 1814 2128 2488 3501
Size: 208 243 43 123 1065 314 360 1013
Cuts at: 3501 3746 132
Size: 245 1089
Fragments arranged by size:
1089 1065 1013 360 314 245 243 208
123 43
NaeI GCC'GGC
Cuts at: 2884 2884
Size: 4703
NarI GG'CG_CC
Cuts at: 217 2134 2382 217
Size: 1917 248 2538
Fragments arranged by size:
2538 1917 248
NgoMIV G'CCGG_C
Cuts at: 2882 2882
Size: 4703
NspI r_CATG'y
Cuts at: 252 489 1756 2256 3163 252
Size: 237 1267 500 907 1792
Fragments arranged by size:
1792 1267 907 500 237
PasI CC'CwG_GG
Cuts at: 609 609
Size: 4703
PciI A'CATG_T
Cuts at: 485 3159 485
Size: 2674 2029
PfoI T'CCnGG_A
Cuts at: 785 785
Size: 4703
PmeI GTTT'AAAC
Cuts at: 1702 1702
Size: 4703
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3842 3874 3842
Size: 32 4671
PsiI TTA'TAA
Cuts at: 821 821
Size: 4703
PstI C_TGCA'G
Cuts at: 672 1154 1750 2435 672
Size: 482 596 685 2940
Fragments arranged by size:
2940 685 596 482
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4703
PvuII CAG'CTG
Cuts at: 340 2128 2488 340
Size: 1788 360 2555
Fragments arranged by size:
2555 1788 360
RsrII CG'GwC_CG
Cuts at: 2898 2898
Size: 4703
SacI G_AGCT'C
Cuts at: 1717 1717
Size: 4703
SalI G'TCGA_C
Cuts at: 1740 1740
Size: 4703
SapI GCTCTTCn'nnn_
Cuts at: 2726 2936 2726
Size: 210 4493
SbfI CC_TGCA'GG
Cuts at: 1750 1750
Size: 4703
ScaI AGT'ACT
Cuts at: 190 4220 190
Size: 4030 673
SfcI C'TryA_G
Cuts at: 33 330 668 1150 1746 2431 3424 3615 33
Size: 297 338 482 596 685 993 191 1121
Fragments arranged by size:
1121 993 685 596 482 338 297 191
SfoI GGC'GCC
Cuts at: 218 2135 2383 218
Size: 1917 248 2538
Fragments arranged by size:
2538 1917 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4703
SmaI CCC'GGG
Cuts at: 138 1725 138
Size: 1587 3116
SmlI C'TyrA_G
Cuts at: 1858 2773 3265 3527 1858
Size: 915 492 262 3034
Fragments arranged by size:
3034 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4703
SphI G_CATG'C
Cuts at: 1756 2256 1756
Size: 500 4203
StuI AGG'CCT
Cuts at: 1156 1156
Size: 4703
StyI C'CwwG_G
Cuts at: 164 212 404 533 1837 2191 164
Size: 48 192 129 1304 354 2676
Fragments arranged by size:
2676 1304 354 192 129 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 755 2286 2419 2878 3087 755
Size: 1531 133 459 209 2371
Fragments arranged by size:
2371 1531 459 209 133
TatI w'GTAC_w
Cuts at: 188 559 977 1550 4218 188
Size: 371 418 573 2668 673
Fragments arranged by size:
2668 673 573 418 371
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 680 712 906 938 1336 1368 4220 4252 680
Size: 32 194 32 398 32 2852 32 1131
Fragments arranged by size:
2852 1131 398 194 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1381 2500 1381
Size: 1119 3584
XbaI T'CTAG_A
Cuts at: 1734 1734
Size: 4703
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 783 2198 783
Size: 1415 3288
XmaI C'CCGG_G
Cuts at: 136 1723 136
Size: 1587 3116
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgZI BtsI
ClaI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PasI PciI
PfoI PmeI PpiI PsiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BbsI BfrBI BmtI BsgI BsiWI BsmI
BsmBI BssHII BstEII BstXI BstZ17I Bsu36I BtgI CspCI
EcoRV FseI FspAI HindIII HpaI NcoI NdeI NheI
NotI NruI NsiI PacI PflMI PmlI PpuMI PshAI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI