Restriction map for
pF1KE0354
(Circular) (Six-base) MAPSORT of: pF1KE0354.seq Check: 5454 from: 1 to: 5270
pF1KE0354 5270 bp
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2179 2179
Size: 5270
AatII G_ACGT'C
Cuts at: 563 563
Size: 5270
AccI GT'mk_AC
Cuts at: 2308 2308
Size: 5270
Acc65I G'GTAC_C
Cuts at: 2286 2286
Size: 5270
AclI AA'CG_TT
Cuts at: 5110 5110
Size: 5270
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 3095 3527 4274 4542 3095
Size: 432 747 268 3823
Fragments arranged by size:
3823 747 432 268
AflIII A'CryG_T
Cuts at: 395 884 1460 2622 3726 4703 395
Size: 489 576 1162 1104 977 962
Fragments arranged by size:
1162 1104 977 962 576 489
AgeI A'CCGG_T
Cuts at: 3658 3658
Size: 5270
AhdI GACnn_n'nnGTC
Cuts at: 1722 1722
Size: 5270
AleI CACnn'nnGTG
Cuts at: 394 931 1687 394
Size: 537 756 3977
Fragments arranged by size:
3977 756 537
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1051 1083 1051
Size: 32 5238
AlwNI CAG_nnn'CTG
Cuts at: 1782 1895 1908 2199 2575 4142 1782
Size: 113 13 291 376 1567 2910
Fragments arranged by size:
2910 1567 376 291 113 13
ApaI G_GGCC'C
Cuts at: 642 1147 1316 1351 1738 1860 642
Size: 505 169 35 387 122 4052
Fragments arranged by size:
4052 505 387 169 122 35
ApaLI G'TGCA_C
Cuts at: 1377 4040 1377
Size: 2663 2607
ApoI r'AATT_y
Cuts at: 2274 4623 4879 2274
Size: 2349 256 2665
Fragments arranged by size:
2665 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5270
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5270
AvaI C'yCGr_G
Cuts at: 2290 2290
Size: 5270
BamHI G'GATC_C
Cuts at: 236 2295 236
Size: 2059 3211
BanI G'GyrC_C
Cuts at: 173 823 1415 1614 2286 2700 2948 2983 173
Size: 650 592 199 672 414 248 35 2460
Fragments arranged by size:
2460 672 650 592 414 248 199 35
BanII G_rGCy'C
Cuts at: 642 1132 1147 1316 1351 1738 1860 1889 2284
Size: 490 15 169 35 387 122 29 395
Cuts at: 2284 3314 642
Size: 1030 2598
Fragments arranged by size:
2598 1030 490 395 387 169 122 35
29 15
BbeI G_GCGC'C
Cuts at: 177 827 1419 2704 2952 177
Size: 650 592 1285 248 2495
Fragments arranged by size:
2495 1285 650 592 248
BbsI GAAGACnn'nnnn_
Cuts at: 383 513 1656 383
Size: 130 1143 3997
Fragments arranged by size:
3997 1143 130
BbvCI CC'TCA_GC
Cuts at: 1555 2041 1555
Size: 486 4784
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 169 203 169
Size: 34 5236
BciVI GTATCCnnnnn_n'
Cuts at: 467 3168 3929 5270 467
Size: 2701 761 1341 467
Fragments arranged by size:
2701 1341 761 467
BclI T'GATC_A
Cuts at: 1706 2790 1706
Size: 1084 4186
BglII A'GATC_T
Cuts at: 1115 2785 4822 1115
Size: 1670 2037 1563
Fragments arranged by size:
2037 1670 1563
BlpI GC'TnA_GC
Cuts at: 356 1126 2382 356
Size: 770 1256 3244
Fragments arranged by size:
3244 1256 770
Bme1580I G_kGCm'C
Cuts at: 642 904 1147 1316 1344 1351 1381 1738 1860
Size: 262 243 169 28 7 30 357 122
Cuts at: 1860 2895 2988 4044 642
Size: 1035 93 1056 1868
Fragments arranged by size:
1868 1056 1035 357 262 243 169 122
93 30 28 7
BmgBI CAC'GTC
Cuts at: 396 1173 1572 1704 396
Size: 777 399 132 3962
Fragments arranged by size:
3962 777 399 132
BmrI ACTGGGnnnn_n'
Cuts at: 2482 2563 2898 5042 2482
Size: 81 335 2144 2710
Fragments arranged by size:
2710 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2031 3637 2031
Size: 1606 3664
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 670 1008 1297 1714 1771 1804 1808 1864 1996
Size: 338 289 417 57 33 4 56 132
Cuts at: 1996 670
Size: 3944
Fragments arranged by size:
3944 417 338 289 132 57 56 33
4
Bpu10I CC'TnA_GC
Cuts at: 1555 1888 2041 1555
Size: 333 153 4784
Fragments arranged by size:
4784 333 153
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2446 3325 3817 4115 2446
Size: 879 492 298 3601
Fragments arranged by size:
3601 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4974 4974
Size: 5270
BsaAI yAC'GTr
Cuts at: 1461 2623 1461
Size: 1162 4108
BsaBI GATnn'nnATC
Cuts at: 6 2784 4821 6
Size: 2778 2037 455
Fragments arranged by size:
2778 2037 455
BsaHI Gr'CG_yC
Cuts at: 174 518 560 824 1416 2701 2949 3651 5147
Size: 344 42 264 592 1285 248 702 1496
Cuts at: 5147 174
Size: 297
Fragments arranged by size:
1496 1285 702 592 344 297 264 248
42
BsaWI w'CCGG_w
Cuts at: 2459 2980 3658 3932 4079 4490 2459
Size: 521 678 274 147 411 3239
Fragments arranged by size:
3239 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 399 429 1744 1756 1774 1786 399
Size: 30 1315 12 18 12 3883
Fragments arranged by size:
3883 1315 30 18 12 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 166 1084 1303 1492 1807 166
Size: 918 219 189 315 3629
Fragments arranged by size:
3629 918 315 219 189
BseYI C'CCAG_C
Cuts at: 157 294 1007 1140 1147 1193 1325 1350 1485
Size: 137 713 133 7 46 132 25 135
Cuts at: 1485 1755 2695 3453 4030 4526 157
Size: 270 940 758 577 496 901
Fragments arranged by size:
940 901 758 713 577 496 270 137
135 133 132 46 25 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1132 1132
Size: 5270
BsiEI CG_ry'CG
Cuts at: 104 1545 1688 2812 2858 4066 104
Size: 1441 143 1124 46 1208 1308
Fragments arranged by size:
1441 1308 1208 1124 143 46
BsiHKAI G_wGCw'C
Cuts at: 752 1381 1624 2284 3062 3252 4044 752
Size: 629 243 660 778 190 792 1978
Fragments arranged by size:
1978 792 778 660 629 243 190
BsmI GAATG_Cn'
Cuts at: 2061 2061
Size: 5270
Bsp1286I G_dGCh'C
Cuts at: 642 752 904 1132 1147 1316 1344 1351 1381
Size: 110 152 228 15 169 28 7 30
Cuts at: 1381 1624 1738 1860 1889 2284 2895 2988 3062
Size: 243 114 122 29 395 611 93 74
Cuts at: 3062 3252 3314 4044 642
Size: 190 62 730 1868
Fragments arranged by size:
1868 730 611 395 243 228 190 169
152 122 114 110 93 74 62 30
29 28 15 7
BspEI T'CCGG_A
Cuts at: 4490 4490
Size: 5270
BspHI T'CATG_A
Cuts at: 4446 4446
Size: 5270
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1727 1751 2179 2320 2534 2836 3217 1727
Size: 24 428 141 214 302 381 3780
Fragments arranged by size:
3780 428 381 302 214 141 24
BsrBI CCG'CTC
Cuts at: 50 1547 2142 3562 3616 5100 5265 50
Size: 1497 595 1420 54 1484 165 55
Fragments arranged by size:
1497 1484 1420 595 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1835 3182 1835
Size: 1347 3923
BsrFI r'CCGG_y
Cuts at: 1409 1633 3268 3449 3658 1409
Size: 224 1635 181 209 3021
Fragments arranged by size:
3021 1635 224 209 181
BssHII G'CGCG_C
Cuts at: 1536 1536
Size: 5270
BssSI C'ACGA_G
Cuts at: 142 817 3541 3899 142
Size: 675 2724 358 1513
Fragments arranged by size:
2724 1513 675 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1908 2056 2180 2647 1908
Size: 148 124 467 4531
Fragments arranged by size:
4531 467 148 124
BstBI TT'CG_AA
Cuts at: 3631 3631
Size: 5270
BstEII G'GTnAC_C
Cuts at: 129 129
Size: 5270
BstYI r'GATC_y
Cuts at: 1 236 1115 2295 2762 2785 3120 3366 4378
Size: 235 879 1180 467 23 335 246 1012
Cuts at: 4378 4464 4476 4822 1
Size: 86 12 346 449
Fragments arranged by size:
1180 1012 879 467 449 346 335 246
235 86 23 12
BtgI C'CryG_G
Cuts at: 107 672 958 1688 107
Size: 565 286 730 3689
Fragments arranged by size:
3689 730 565 286
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1049 3220 3401 3564 1049
Size: 2171 181 163 2755
Fragments arranged by size:
2755 2171 181 163
BtsI GCAGTG_nn'
Cuts at: 126 827 1918 2184 2513 126
Size: 701 1091 266 329 2883
Fragments arranged by size:
2883 1091 701 329 266
DraI TTT'AAA
Cuts at: 2269 2269
Size: 5270
DraIII CAC_nnn'GTG
Cuts at: 912 1028 912
Size: 116 5154
DrdI GACnn_nn'nnGTC
Cuts at: 2976 3834 2976
Size: 858 4412
EaeI y'GGCC_r
Cuts at: 546 699 803 1685 2252 2855 3029 3420 3447
Size: 153 104 882 567 603 174 391 27
Cuts at: 3447 4792 546
Size: 1345 1024
Fragments arranged by size:
1345 1024 882 603 567 391 174 153
104 27
EagI C'GGCC_G
Cuts at: 1685 2855 1685
Size: 1170 4100
EarI CTCTTCn'nnn_
Cuts at: 857 3293 3503 857
Size: 2436 210 2624
Fragments arranged by size:
2624 2436 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3478 3788 3934 5080 3478
Size: 310 146 1146 3668
Fragments arranged by size:
3668 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2282 2282
Size: 5270
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 670 1008 1297 1714 1771 1804 1808 1864 1996
Size: 338 289 417 57 33 4 56 132
Cuts at: 1996 3095 3527 4274 4542 670
Size: 1099 432 747 268 1398
Fragments arranged by size:
1398 1099 747 432 417 338 289 268
132 57 56 33 4
EcoNI CCTnn'n_nnAGG
Cuts at: 652 805 874 652
Size: 153 69 5048
Fragments arranged by size:
5048 153 69
EcoO109I rG'GnC_Cy
Cuts at: 338 639 867 983 1144 1312 1313 1347 1431
Size: 301 228 116 161 168 1 34 84
Cuts at: 1431 1734 1735 1856 1892 2409 338
Size: 303 1 121 36 517 3199
Fragments arranged by size:
3199 517 303 301 228 168 161 121
116 84 36 34 1 1
EcoRI G'AATT_C
Cuts at: 2274 2274
Size: 5270
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3289 3289
Size: 5270
FspI TGC'GCA
Cuts at: 3051 3051
Size: 5270
HaeII r_GCGC'y
Cuts at: 177 827 1419 1562 1677 2704 2952 3974 4953
Size: 650 592 143 115 1027 248 1022 979
Cuts at: 4953 177
Size: 494
Fragments arranged by size:
1027 1022 979 650 592 494 248 143
115
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1239 1271 1708 1740 3112 3142 3144 3174 1239
Size: 32 437 32 1372 30 2 30 3335
Fragments arranged by size:
3335 1372 437 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2309 2309
Size: 5270
KasI G'GCGC_C
Cuts at: 173 823 1415 2700 2948 173
Size: 650 592 1285 248 2495
Fragments arranged by size:
2495 1285 650 592 248
KpnI G_GTAC'C
Cuts at: 2290 2290
Size: 5270
MluI A'CGCG_T
Cuts at: 884 4703 884
Size: 3819 1451
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 144 1742 3941 4125 144
Size: 1598 2199 184 1289
Fragments arranged by size:
2199 1598 1289 184
MscI TGG'CCA
Cuts at: 805 2254 3031 805
Size: 1449 777 3044
Fragments arranged by size:
3044 1449 777
MslI CAynn'nnrTG
Cuts at: 394 707 931 1687 2201 3259 3386 394
Size: 313 224 756 514 1058 127 2278
Fragments arranged by size:
2278 1058 756 514 313 224 127
MspA1I CmG'CkG
Cuts at: 294 551 624 998 1193 1583 1770 1905 2085
Size: 257 73 374 195 390 187 135 180
Cuts at: 2085 2381 2695 3055 4068 4313 294
Size: 296 314 360 1013 245 1251
Fragments arranged by size:
1251 1013 390 374 360 314 296 257
245 195 187 180 135 73
NaeI GCC'GGC
Cuts at: 1635 3451 1635
Size: 1816 3454
NarI GG'CG_CC
Cuts at: 174 824 1416 2701 2949 174
Size: 650 592 1285 248 2495
Fragments arranged by size:
2495 1285 650 592 248
NcoI C'CATG_G
Cuts at: 107 958 107
Size: 851 4419
NgoMIV G'CCGG_C
Cuts at: 1633 3449 1633
Size: 1816 3454
NspI r_CATG'y
Cuts at: 2323 2823 3730 2323
Size: 500 907 3863
Fragments arranged by size:
3863 907 500
PasI CC'CwG_GG
Cuts at: 1316 1316
Size: 5270
PciI A'CATG_T
Cuts at: 3726 3726
Size: 5270
PflMI CCAn_nnn'nTGG
Cuts at: 144 801 1608 2199 144
Size: 657 807 591 3215
Fragments arranged by size:
3215 807 657 591
PfoI T'CCnGG_A
Cuts at: 486 647 1973 486
Size: 161 1326 3783
Fragments arranged by size:
3783 1326 161
PmeI GTTT'AAAC
Cuts at: 2269 2269
Size: 5270
PmlI CAC'GTG
Cuts at: 1461 1461
Size: 5270
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 349 381 4409 4441 349
Size: 32 4028 32 1178
Fragments arranged by size:
4028 1178 32 32
PpuMI rG'GwC_Cy
Cuts at: 1892 1892
Size: 5270
PshAI GACnn'nnGTC
Cuts at: 398 398
Size: 5270
PspOMI G'GGCC_C
Cuts at: 638 1143 1312 1347 1734 1856 638
Size: 505 169 35 387 122 4052
Fragments arranged by size:
4052 505 387 169 122 35
PstI C_TGCA'G
Cuts at: 182 1338 1724 1913 2191 2317 3002 182
Size: 1156 386 189 278 126 685 2450
Fragments arranged by size:
2450 1156 685 386 278 189 126
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5270
PvuII CAG'CTG
Cuts at: 294 998 1770 1905 2085 2695 3055 294
Size: 704 772 135 180 610 360 2509
Fragments arranged by size:
2509 772 704 610 360 180 135
RsrII CG'GwC_CG
Cuts at: 3465 3465
Size: 5270
SacI G_AGCT'C
Cuts at: 2284 2284
Size: 5270
SalI G'TCGA_C
Cuts at: 2307 2307
Size: 5270
SapI GCTCTTCn'nnn_
Cuts at: 3293 3503 3293
Size: 210 5060
SbfI CC_TGCA'GG
Cuts at: 2317 2317
Size: 5270
ScaI AGT'ACT
Cuts at: 4787 4787
Size: 5270
SexAI A'CCwGG_T
Cuts at: 1607 1607
Size: 5270
SfcI C'TryA_G
Cuts at: 33 178 1334 1720 1909 2187 2313 2998 3991
Size: 145 1156 386 189 278 126 685 993
Cuts at: 3991 4182 33
Size: 191 1121
Fragments arranged by size:
1156 1121 993 685 386 278 191 189
145 126
SfoI GGC'GCC
Cuts at: 175 825 1417 2702 2950 175
Size: 650 592 1285 248 2495
Fragments arranged by size:
2495 1285 650 592 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5270
SgrAI Cr'CCGG_yG
Cuts at: 1633 1633
Size: 5270
SmaI CCC'GGG
Cuts at: 2292 2292
Size: 5270
SmlI C'TyrA_G
Cuts at: 2425 3340 3832 4094 2425
Size: 915 492 262 3601
Fragments arranged by size:
3601 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5270
SphI G_CATG'C
Cuts at: 2323 2823 2323
Size: 500 4770
StuI AGG'CCT
Cuts at: 658 1825 658
Size: 1167 4103
StyI C'CwwG_G
Cuts at: 107 279 806 929 958 1185 1427 2404 2758
Size: 172 527 123 29 227 242 977 354
Cuts at: 2758 107
Size: 2619
Fragments arranged by size:
2619 977 527 354 242 227 172 123
29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1185 2853 2986 3445 3654 1185
Size: 1668 133 459 209 2801
Fragments arranged by size:
2801 1668 459 209 133
TatI w'GTAC_w
Cuts at: 4785 4785
Size: 5270
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 874 906 4787 4819 874
Size: 32 3881 32 1325
Fragments arranged by size:
3881 1325 32 32
Tth111I GACn'n_nGTC
Cuts at: 3067 3067
Size: 5270
XbaI T'CTAG_A
Cuts at: 2301 2301
Size: 5270
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 141 2200 2765 141
Size: 2059 565 2646
Fragments arranged by size:
2646 2059 565
XmaI C'CCGG_G
Cuts at: 2290 2290
Size: 5270
XmnI GAAnn'nnTTC
Cuts at: 631 631
Size: 5270
ZraI GAC'GTC
Cuts at: 561 561
Size: 5270
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AleI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AlfI AscI AvrII BaeI BfrBI BglI
BmtI BsiWI BsmBI BsrGI BstXI BstZ17I Bsu36I ClaI
CspCI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PsiI PspXI PsrI
SacII SanDI SfiI SnaBI SrfI SspI SwaI XhoI