Restriction map for
pF1KE0355
(Circular) (Six-base) MAPSORT of: pF1KE0355.seq Check: 5438 from: 1 to: 5006
pF1KE0355 5006 bp
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 941 941
Size: 5006
AatII G_ACGT'C
Cuts at: 1276 1276
Size: 5006
AccI GT'mk_AC
Cuts at: 358 449 1364 2044 358
Size: 91 915 680 3320
Fragments arranged by size:
3320 915 680 91
Acc65I G'GTAC_C
Cuts at: 1910 2022 1910
Size: 112 4894
AclI AA'CG_TT
Cuts at: 4846 4846
Size: 5006
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 343 409 493 604 1351 2831 3263 4010 4278
Size: 66 84 111 747 1480 432 747 268
Cuts at: 4278 343
Size: 1071
Fragments arranged by size:
1480 1071 747 747 432 268 111 84
66
AflIII A'CryG_T
Cuts at: 2358 3462 4439 2358
Size: 1104 977 2925
Fragments arranged by size:
2925 1104 977
AgeI A'CCGG_T
Cuts at: 3394 3394
Size: 5006
AhdI GACnn_n'nnGTC
Cuts at: 1663 1663
Size: 5006
AleI CACnn'nnGTG
Cuts at: 336 443 336
Size: 107 4899
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1228 1260 1228
Size: 32 4974
AlwNI CAG_nnn'CTG
Cuts at: 342 582 969 1266 1329 2311 3878 342
Size: 240 387 297 63 982 1567 1470
Fragments arranged by size:
1567 1470 982 387 297 240 63
ApaI G_GGCC'C
Cuts at: 354 819 1781 354
Size: 465 962 3579
Fragments arranged by size:
3579 962 465
ApaLI G'TGCA_C
Cuts at: 1501 3776 1501
Size: 2275 2731
ApoI r'AATT_y
Cuts at: 2010 4359 4615 2010
Size: 2349 256 2401
Fragments arranged by size:
2401 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5006
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5006
AvaI C'yCGr_G
Cuts at: 203 490 701 1120 1511 1982 2026 203
Size: 287 211 419 391 471 44 3183
Fragments arranged by size:
3183 471 419 391 287 211 44
BamHI G'GATC_C
Cuts at: 2031 2031
Size: 5006
BanI G'GyrC_C
Cuts at: 1807 1910 2022 2436 2684 2719 1807
Size: 103 112 414 248 35 4094
Fragments arranged by size:
4094 414 248 112 103 35
BanII G_rGCy'C
Cuts at: 354 819 1127 1316 1512 1544 1655 1781 2020
Size: 465 308 189 196 32 111 126 239
Cuts at: 2020 3050 354
Size: 1030 2310
Fragments arranged by size:
2310 1030 465 308 239 196 189 126
111 32
BbeI G_GCGC'C
Cuts at: 2440 2688 2440
Size: 248 4758
BbsI GAAGACnn'nnnn_
Cuts at: 646 1339 1745 646
Size: 693 406 3907
Fragments arranged by size:
3907 693 406
BbvCI CC'TCA_GC
Cuts at: 1846 1862 1846
Size: 16 4990
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 765 799 765
Size: 34 4972
BciVI GTATCCnnnnn_n'
Cuts at: 1016 2904 3665 5006 1016
Size: 1888 761 1341 1016
Fragments arranged by size:
1888 1341 1016 761
BclI T'GATC_A
Cuts at: 2526 2526
Size: 5006
BglI GCCn_nnn'nGGC
Cuts at: 118 172 607 1516 1615 118
Size: 54 435 909 99 3509
Fragments arranged by size:
3509 909 435 99 54
BglII A'GATC_T
Cuts at: 494 2521 4558 494
Size: 2027 2037 942
Fragments arranged by size:
2037 2027 942
BlpI GC'TnA_GC
Cuts at: 937 2118 937
Size: 1181 3825
Bme1580I G_kGCm'C
Cuts at: 302 354 819 1505 1781 1959 2631 2724 3780
Size: 52 465 686 276 178 672 93 1056
Cuts at: 3780 302
Size: 1528
Fragments arranged by size:
1528 1056 686 672 465 276 178 93
52
BmrI ACTGGGnnnn_n'
Cuts at: 348 2218 2299 2634 4778 348
Size: 1870 81 335 2144 576
Fragments arranged by size:
2144 1870 576 335 81
BmtI G_CTAG'C
Cuts at: 331 331
Size: 5006
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 487 579 3373 487
Size: 92 2794 2120
Fragments arranged by size:
2794 2120 92
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 364 574 700 733 856 1276 1690 1714 1909
Size: 210 126 33 123 420 414 24 195
Cuts at: 1909 364
Size: 3461
Fragments arranged by size:
3461 420 414 210 195 126 123 33
24
Bpu10I CC'TnA_GC
Cuts at: 1525 1846 1862 1960 1525
Size: 321 16 98 4571
Fragments arranged by size:
4571 321 98 16
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 868 2182 3061 3553 3851 868
Size: 1314 879 492 298 2023
Fragments arranged by size:
2023 1314 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 930 1196 4710 930
Size: 266 3514 1226
Fragments arranged by size:
3514 1226 266
BsaAI yAC'GTr
Cuts at: 1968 2359 1968
Size: 391 4615
BsaBI GATnn'nnATC
Cuts at: 6 2520 4557 6
Size: 2514 2037 455
Fragments arranged by size:
2514 2037 455
BsaHI Gr'CG_yC
Cuts at: 547 1273 2437 2685 3387 4883 547
Size: 726 1164 248 702 1496 670
Fragments arranged by size:
1496 1164 726 702 670 248
BsaWI w'CCGG_w
Cuts at: 2195 2716 3394 3668 3815 4226 2195
Size: 521 678 274 147 411 2975
Fragments arranged by size:
2975 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 229 259 229
Size: 30 4976
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 243 571 721 745 1360 1579 1863 1906 1939
Size: 328 150 24 615 219 284 43 33
Cuts at: 1939 243
Size: 3310
Fragments arranged by size:
3310 615 328 284 219 150 43 33
24
BseYI C'CCAG_C
Cuts at: 286 422 622 1126 1316 2431 3189 3766 4262
Size: 136 200 504 190 1115 758 577 496
Cuts at: 4262 286
Size: 1030
Fragments arranged by size:
1115 1030 758 577 504 496 200 190
136
BsiEI CG_ry'CG
Cuts at: 104 122 365 435 959 2548 2594 3802 104
Size: 18 243 70 524 1589 46 1208 1308
Fragments arranged by size:
1589 1308 1208 524 243 70 46 18
BsiHKAI G_wGCw'C
Cuts at: 988 1505 1544 1655 2020 2798 2988 3780 988
Size: 517 39 111 365 778 190 792 2214
Fragments arranged by size:
2214 792 778 517 365 190 111 39
BsmI GAATG_Cn'
Cuts at: 1557 1557
Size: 5006
Bsp1286I G_dGCh'C
Cuts at: 302 354 819 988 1127 1316 1505 1512 1544
Size: 52 465 169 139 189 189 7 32
Cuts at: 1544 1655 1781 1959 2020 2631 2724 2798 2988
Size: 111 126 178 61 611 93 74 190
Cuts at: 2988 3050 3780 302
Size: 62 730 1528
Fragments arranged by size:
1528 730 611 465 190 189 189 178
169 139 126 111 93 74 62 61
52 32 7
BspEI T'CCGG_A
Cuts at: 4226 4226
Size: 5006
BspHI T'CATG_A
Cuts at: 4182 4182
Size: 5006
BspMI ACCTGCnnnn'nnnn_
Cuts at: 941 1826 2056 2270 2572 2953 941
Size: 885 230 214 302 381 2994
Fragments arranged by size:
2994 885 381 302 230 214
BsrBI CCG'CTC
Cuts at: 50 1281 1853 3298 3352 4836 5001 50
Size: 1231 572 1445 54 1484 165 55
Fragments arranged by size:
1484 1445 1231 572 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1101 2918 1101
Size: 1817 3189
BsrFI r'CCGG_y
Cuts at: 190 197 430 737 3004 3185 3394 190
Size: 7 233 307 2267 181 209 1802
Fragments arranged by size:
2267 1802 307 233 209 181 7
BsrGI T'GTAC_A
Cuts at: 992 1490 992
Size: 498 4508
BssSI C'ACGA_G
Cuts at: 910 1903 3277 3635 910
Size: 993 1374 358 2281
Fragments arranged by size:
2281 1374 993 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2383 2383
Size: 5006
BstBI TT'CG_AA
Cuts at: 1397 3367 1397
Size: 1970 3036
BstXI CCAn_nnnn'nTGG
Cuts at: 183 183
Size: 5006
BstYI r'GATC_y
Cuts at: 1 494 1349 2031 2498 2521 2856 3102 4114
Size: 493 855 682 467 23 335 246 1012
Cuts at: 4114 4200 4212 4558 1
Size: 86 12 346 449
Fragments arranged by size:
1012 855 682 493 467 449 346 335
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 450 450
Size: 5006
Bsu36I CC'TnA_GG
Cuts at: 226 970 1604 226
Size: 744 634 3628
Fragments arranged by size:
3628 744 634
BtgI C'CryG_G
Cuts at: 107 1138 1184 1296 1337 107
Size: 1031 46 112 41 3776
Fragments arranged by size:
3776 1031 112 46 41
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1773 2956 3137 3300 1773
Size: 1183 181 163 3479
Fragments arranged by size:
3479 1183 181 163
BtsI GCAGTG_nn'
Cuts at: 343 988 1945 2249 343
Size: 645 957 304 3100
Fragments arranged by size:
3100 957 645 304
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1551 1586 1551
Size: 35 4971
DraI TTT'AAA
Cuts at: 2005 2005
Size: 5006
DrdI GACnn_nn'nnGTC
Cuts at: 2712 3570 2712
Size: 858 4148
EaeI y'GGCC_r
Cuts at: 110 119 362 432 859 1994 2591 2765 3156
Size: 9 243 70 427 1135 597 174 391
Cuts at: 3156 3183 4528 110
Size: 27 1345 588
Fragments arranged by size:
1345 1135 597 588 427 391 243 174
70 27 9
EagI C'GGCC_G
Cuts at: 119 362 432 2591 119
Size: 243 70 2159 2534
Fragments arranged by size:
2534 2159 243 70
EarI CTCTTCn'nnn_
Cuts at: 3029 3239 3029
Size: 210 4796
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3214 3524 3670 4816 3214
Size: 310 146 1146 3404
Fragments arranged by size:
3404 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1542 1653 2018 1542
Size: 111 365 4530
Fragments arranged by size:
4530 365 111
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 343 364 409 493 574 604 700 733 856
Size: 21 45 84 81 30 96 33 123
Cuts at: 856 1276 1351 1690 1714 1909 2831 3263 4010
Size: 420 75 339 24 195 922 432 747
Cuts at: 4010 4278 343
Size: 268 1071
Fragments arranged by size:
1071 922 747 432 420 339 268 195
123 96 84 81 75 45 33 30
24 21
EcoNI CCTnn'n_nnAGG
Cuts at: 1996 1996
Size: 5006
EcoO109I rG'GnC_Cy
Cuts at: 350 816 900 1251 1778 2145 350
Size: 466 84 351 527 367 3211
Fragments arranged by size:
3211 527 466 367 351 84
EcoRI G'AATT_C
Cuts at: 2010 2010
Size: 5006
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3025 3025
Size: 5006
FspI TGC'GCA
Cuts at: 2787 2787
Size: 5006
HaeII r_GCGC'y
Cuts at: 155 738 1001 2440 2688 3710 4689 155
Size: 583 263 1439 248 1022 979 472
Fragments arranged by size:
1439 1022 979 583 472 263 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 642 674 1201 1233 1649 1681 2848 2878 2880
Size: 32 527 32 416 32 1167 30 2
Cuts at: 2880 2910 642
Size: 30 2738
Fragments arranged by size:
2738 1167 527 416 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 359 2045 359
Size: 1686 3320
HindIII A'AGCT_T
Cuts at: 160 370 505 160
Size: 210 135 4661
Fragments arranged by size:
4661 210 135
KasI G'GCGC_C
Cuts at: 2436 2684 2436
Size: 248 4758
KpnI G_GTAC'C
Cuts at: 1914 2026 1914
Size: 112 4894
MluI A'CGCG_T
Cuts at: 4439 4439
Size: 5006
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1197 1853 3677 3861 1197
Size: 656 1824 184 2342
Fragments arranged by size:
2342 1824 656 184
MscI TGG'CCA
Cuts at: 1996 2767 1996
Size: 771 4235
MslI CAynn'nnrTG
Cuts at: 336 443 2995 3122 336
Size: 107 2552 127 2220
Fragments arranged by size:
2552 2220 127 107
MspA1I CmG'CkG
Cuts at: 115 144 409 666 813 1140 1320 1329 1339
Size: 29 265 257 147 327 180 9 10
Cuts at: 1339 1414 1430 1467 1798 1866 2117 2431 2791
Size: 75 16 37 331 68 251 314 360
Cuts at: 2791 3804 4049 115
Size: 1013 245 1072
Fragments arranged by size:
1072 1013 360 331 327 314 265 257
251 245 180 147 75 68 37 29
16 10 9
NaeI GCC'GGC
Cuts at: 432 739 3187 432
Size: 307 2448 2251
Fragments arranged by size:
2448 2251 307
NarI GG'CG_CC
Cuts at: 2437 2685 2437
Size: 248 4758
NcoI C'CATG_G
Cuts at: 107 107
Size: 5006
NgoMIV G'CCGG_C
Cuts at: 430 737 3185 430
Size: 307 2448 2251
Fragments arranged by size:
2448 2251 307
NheI G'CTAG_C
Cuts at: 327 327
Size: 5006
NspI r_CATG'y
Cuts at: 1110 2059 2559 3466 1110
Size: 949 500 907 2650
Fragments arranged by size:
2650 949 907 500
PasI CC'CwG_GG
Cuts at: 1203 1203
Size: 5006
PciI A'CATG_T
Cuts at: 3462 3462
Size: 5006
PfoI T'CCnGG_A
Cuts at: 749 833 1667 749
Size: 84 834 4088
Fragments arranged by size:
4088 834 84
PmeI GTTT'AAAC
Cuts at: 2005 2005
Size: 5006
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4145 4177 4145
Size: 32 4974
PpuMI rG'GwC_Cy
Cuts at: 900 1251 900
Size: 351 4655
PspOMI G'GGCC_C
Cuts at: 350 815 1777 350
Size: 465 962 3579
Fragments arranged by size:
3579 962 465
PstI C_TGCA'G
Cuts at: 149 408 572 983 1325 1797 1952 2053 2738
Size: 259 164 411 342 472 155 101 685
Cuts at: 2738 149
Size: 2417
Fragments arranged by size:
2417 685 472 411 342 259 164 155
101
PvuI CG_AT'CG
Cuts at: 104 959 104
Size: 855 4151
PvuII CAG'CTG
Cuts at: 409 1320 1329 1414 1798 1866 2431 2791 409
Size: 911 9 85 384 68 565 360 2624
Fragments arranged by size:
2624 911 565 384 360 85 68 9
RsrII CG'GwC_CG
Cuts at: 3201 3201
Size: 5006
SacI G_AGCT'C
Cuts at: 1544 1655 2020 1544
Size: 111 365 4530
Fragments arranged by size:
4530 365 111
SacII CC_GC'GG
Cuts at: 1141 1340 1141
Size: 199 4807
SalI G'TCGA_C
Cuts at: 357 2043 357
Size: 1686 3320
SapI GCTCTTCn'nnn_
Cuts at: 3029 3239 3029
Size: 210 4796
SbfI CC_TGCA'GG
Cuts at: 2053 2053
Size: 5006
ScaI AGT'ACT
Cuts at: 4523 4523
Size: 5006
SfcI C'TryA_G
Cuts at: 33 145 404 568 661 979 1321 1545 1793
Size: 112 259 164 93 318 342 224 248
Cuts at: 1793 1948 2049 2734 3727 3918 33
Size: 155 101 685 993 191 1121
Fragments arranged by size:
1121 993 685 342 318 259 248 224
191 164 155 112 101 93
SfiI GGCCn_nnn'nGGCC
Cuts at: 118 1615 118
Size: 1497 3509
SfoI GGC'GCC
Cuts at: 2438 2686 2438
Size: 248 4758
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5006
SmaI CCC'GGG
Cuts at: 205 703 1513 1984 2028 205
Size: 498 810 471 44 3183
Fragments arranged by size:
3183 810 498 471 44
SmlI C'TyrA_G
Cuts at: 883 2161 3076 3568 3830 883
Size: 1278 915 492 262 2059
Fragments arranged by size:
2059 1278 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5006
SphI G_CATG'C
Cuts at: 1110 2059 2559 1110
Size: 949 500 3557
Fragments arranged by size:
3557 949 500
StuI AGG'CCT
Cuts at: 562 1403 1991 562
Size: 841 588 3577
Fragments arranged by size:
3577 841 588
StyI C'CwwG_G
Cuts at: 107 1421 1610 1997 2140 2494 107
Size: 1314 189 387 143 354 2619
Fragments arranged by size:
2619 1314 387 354 189 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2589 2722 3181 3390 2589
Size: 133 459 209 4205
Fragments arranged by size:
4205 459 209 133
TatI w'GTAC_w
Cuts at: 992 1490 4521 992
Size: 498 3031 1477
Fragments arranged by size:
3031 1477 498
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 229 261 1710 1742 4523 4555 229
Size: 32 1449 32 2781 32 680
Fragments arranged by size:
2781 1449 680 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2803 2803
Size: 5006
XbaI T'CTAG_A
Cuts at: 2037 2037
Size: 5006
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2501 2501
Size: 5006
XmaI C'CCGG_G
Cuts at: 203 701 1511 1982 2026 203
Size: 498 810 471 44 3183
Fragments arranged by size:
3183 810 498 471 44
ZraI GAC'GTC
Cuts at: 1274 1274
Size: 5006
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AleI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NspI PasI PciI PfoI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AlfI AscI AvrII BaeI BfrBI BmgBI
BsgI BsiWI BsmBI BssHII BstEII ClaI DraIII EcoRV
FseI FspAI HpaI MfeI NdeI NotI NruI NsiI
PacI PflMI PmlI PshAI PsiI PspXI PsrI SanDI
SexAI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI